Isolation and characterization of ribosomal protein S6 kinase gene’s cDNA in Triticum aestivum
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Keywords:
miRNA, mRNA, miR396, растение.miRNA, өсімдікAbstract
In present work cDNA of Triticum aestivum gene encoding homologue of ribosomal protein S6 kinase (S6K) was isolated. Analysis of TaS6K protein revealed conservative elements in kinase domain characteristic of all S6 kinases:activation loop, hydrophobic and turn motives. It was shown that Ser-466 in C-terminal HM-motif of TaS6K is a putative site of phosphorylation by T.aestivum TOR kinase. The phosphorylation of Ser-466 TaS6K is inhibited in presence of TOR-specific inhibitor pp242 in aleuronic layer of wheat grain. Also a higher level of activated TaS6K in aleuronic cells treated with gibberellin compared to abscisic acid was observed as an indication of possible participation of TOR signaling pathway in gibberellin-activated processes during germination of wheat grain.References
1. Ding Y., Tao Y., Zhu C. Emerging roles of microRNAs in the mediation of drought stress response in plants // J. Exp. Bot. -2013. - Vol. 64. - P. 3077-3086.
2. Hwang D-G, Park J.H., Lim J.Y., Kim D., Choi Y., et al. The hot pepper (Capsicum annuum) microRNA transcriptome reveals
novel and conserved targets: a foundation for understanding microRNA functional roles in hot pepper // PLoS ONE. - 2013. - Vol. 8.
- e64238. - doi:10.1371/journal.pone.0064238.
3. Minorsky P.V. Syncytium formation by cyst nematodes: involvement of a microRNA // Plant Physiol. - 2012. - Vol. 159. - P. 1-2.
4. Hwang E.-W., Shin S.-J., Kwon H.-B. Identification of microRNAs and their putative targets that respond to drought stress in
Solanum tuberosum // J. Korean Soc. Appl. Biol. Chem. - 2011. - Vol. 54. - P. 317-324.
5. Zhang Y., Yin Z., Feng X., Shen F. Differential expression of microRNAs between 21A genetic male sterile line and its
maintainer line in cotton (Gossypium hirsutum L.) // J. of Plant Studies. - 2014. - Vol. 3. - P. 13-27.
6. Xia R., Zhu H., An Y.-G., Beers E.P., Liu Z. Apple miRNAs and tasiRNAs with novel regulatory networks // Genome Biol. -
2012. - Vol.13. - P. 47.
7. Haga N., Kato K., Murase M., Araki S., Kubo M., Demura T., Suzuki K., Muller I., Voss U., Jurgens G. et al. R1R2R3-Myb
proteins positively regulate cytokinesis through activation of KNOLLE transcription in Arabidopsis thaliana // Development. - 2007.
- Vol. 134. - P. 1101-1110.
8. Kim J.H. and Kende H.A. Transcriptional coactivator, AtGIF1, is involved in regulating leaf growth and morphology in
Arabidopsis // Proc. Natl. Acad. Sci. USA. - 2004. - Vol. 101. - P. 13374-13379.
9. Kim J.H. and Lee B.H. Growth-regulating factor 4 of Arabidopsis thaliana is required for development of leaves, cotyledons, and
shoot apical meristem // J. Plant Biol. - 2006. - Vol. 49. - P.463-468.
10. Kim J.H., Choi D., Kende H. The AtGRF family of putative transcription factors is involved in leaf and cotyledon growth in
Arabidopsis // Plant J. - 2003. - Vol. 36. - P. 94-104.
11. Horiguchi G., Kim G.T. and Tsukaya H. The transcription factor AtGRF5 and the transcription coactivator AN3 regulate cell
proliferation in leaf primordia of Arabidopsis thaliana // Plant J. - 2005. - Vol. 43. - P. 68-78.
12. Rodriguez R.E., Mecchia M.A., Debernardi J.M., Schommer C., Weigel D., Palatnik J.F. Control of cell proliferation in
Arabidopsis thaliana by microRNA miR396 // Development. - 2010. - Vol.137. - P. 103-112.
13. Liu D., Song Y., Chen Z. and Yu D. Ectopic expression of miR396 suppresses GRF target gene expression and alters leaf growth
in Arabidopsis // Physiol. Plant. - 2009.- Vol. 136. - P. 223-236.
14. Palatnik J.F., Allen E., Wu X., Schommer C., Schwab R., Carrington J.C., Weigel, D. Control of leaf morphogenesis by
microRNAs // Nature. - 2003. - Vol. 425. - P. 257-263.
2. Hwang D-G, Park J.H., Lim J.Y., Kim D., Choi Y., et al. The hot pepper (Capsicum annuum) microRNA transcriptome reveals
novel and conserved targets: a foundation for understanding microRNA functional roles in hot pepper // PLoS ONE. - 2013. - Vol. 8.
- e64238. - doi:10.1371/journal.pone.0064238.
3. Minorsky P.V. Syncytium formation by cyst nematodes: involvement of a microRNA // Plant Physiol. - 2012. - Vol. 159. - P. 1-2.
4. Hwang E.-W., Shin S.-J., Kwon H.-B. Identification of microRNAs and their putative targets that respond to drought stress in
Solanum tuberosum // J. Korean Soc. Appl. Biol. Chem. - 2011. - Vol. 54. - P. 317-324.
5. Zhang Y., Yin Z., Feng X., Shen F. Differential expression of microRNAs between 21A genetic male sterile line and its
maintainer line in cotton (Gossypium hirsutum L.) // J. of Plant Studies. - 2014. - Vol. 3. - P. 13-27.
6. Xia R., Zhu H., An Y.-G., Beers E.P., Liu Z. Apple miRNAs and tasiRNAs with novel regulatory networks // Genome Biol. -
2012. - Vol.13. - P. 47.
7. Haga N., Kato K., Murase M., Araki S., Kubo M., Demura T., Suzuki K., Muller I., Voss U., Jurgens G. et al. R1R2R3-Myb
proteins positively regulate cytokinesis through activation of KNOLLE transcription in Arabidopsis thaliana // Development. - 2007.
- Vol. 134. - P. 1101-1110.
8. Kim J.H. and Kende H.A. Transcriptional coactivator, AtGIF1, is involved in regulating leaf growth and morphology in
Arabidopsis // Proc. Natl. Acad. Sci. USA. - 2004. - Vol. 101. - P. 13374-13379.
9. Kim J.H. and Lee B.H. Growth-regulating factor 4 of Arabidopsis thaliana is required for development of leaves, cotyledons, and
shoot apical meristem // J. Plant Biol. - 2006. - Vol. 49. - P.463-468.
10. Kim J.H., Choi D., Kende H. The AtGRF family of putative transcription factors is involved in leaf and cotyledon growth in
Arabidopsis // Plant J. - 2003. - Vol. 36. - P. 94-104.
11. Horiguchi G., Kim G.T. and Tsukaya H. The transcription factor AtGRF5 and the transcription coactivator AN3 regulate cell
proliferation in leaf primordia of Arabidopsis thaliana // Plant J. - 2005. - Vol. 43. - P. 68-78.
12. Rodriguez R.E., Mecchia M.A., Debernardi J.M., Schommer C., Weigel D., Palatnik J.F. Control of cell proliferation in
Arabidopsis thaliana by microRNA miR396 // Development. - 2010. - Vol.137. - P. 103-112.
13. Liu D., Song Y., Chen Z. and Yu D. Ectopic expression of miR396 suppresses GRF target gene expression and alters leaf growth
in Arabidopsis // Physiol. Plant. - 2009.- Vol. 136. - P. 223-236.
14. Palatnik J.F., Allen E., Wu X., Schommer C., Schwab R., Carrington J.C., Weigel, D. Control of leaf morphogenesis by
microRNAs // Nature. - 2003. - Vol. 425. - P. 257-263.
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Sagaidak, A. I., Pinsky, I. V., Ivashchenko, A. T., Regnier, M., & Bari, A. A. (2015). Isolation and characterization of ribosomal protein S6 kinase gene’s cDNA in Triticum aestivum. Experimental Biology, 59(3/1), 289–291. Retrieved from https://bb.kaznu.kz/index.php/biology/article/view/778
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Physical and chemical biology and nanotechnologies
