Construction of recombinant Saccharomyces cerevisiae strains containing cellobiohydrolase gene of Lentinula edodes into the chromosomal HO locus.
Keywords:
cellobiohydrolase, Lentinula edodes, Saccharomyces cerevisiae, genome integration, α-factor,Abstract
Using genetic engineering techniques the pHO-GAPDH-α-cel7A-myc-6His-KanMX4-HO integrated construction, including cellobiohydrolase gene cel7A of the Lentinula edodes with the signal sequence of yeast α-factor, Mycepitope and 6xHis tail have been designed. The new stable yeast strain efficiently expressing recombinant cellobiohydrolase CEL7A of the fungus Lentinula edodes have been obtained. Chromosomal integration of the cel7A gene at the HO locus was confirmed by PCR verification. Keywords:References
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фермента // Известия НАН РК. Серия биологическая и медицинская. - 2012. - №3(291). - С. 39-46.
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2 Teeri T.T. Crystalline cellulose degradation: new insight into the function of cellobiohydrolases // Trends in Biotechnology.– 1997. – V. 15. – P. 160-167.
3 Bhat M.K. Cellulases and related enzymes in biotechnology // Biotechnol. Adv. – 2000. – Vol.18. – P.355-383.
4 Lynd L.R., van Zyl W.H., McBride J. E., Laser M. Consolidated bioprocessing of cellulosic biomass: an update //Current Opinion in Biotechnology. -2005. -Vol.16. - № 5. – P. 577–583.
5 Hong J., Tamaki H., Akiba S., Yamamoto K., Kumagai H. Cloning of a gene encoding a highly stable endo-b-1,4-β-
glucanase from Aspergillus niger and its expression in yeast // J. Biosci. Bioeng. – 2001. –Vol.92. - P. 434-441.
6 Voth W.P., Richards J.D., Shaw J.M., Stillman D.J. Yeast vectors for integration at the HO locus // Nucleic Acids
Res. -2001. –Vol.29. - №12. – P. 354-363.
7 MichaelisS., and Herskowitz I., et al.The a-factor pheromone of Saccharomyces cerevisiae is essential for mating //Mol. Cell Biol. -1988. - Vol.8. - № 3. –Р.1309-18.
8 Strazdis J.R., MacKay V.L. Induction of yeast mating pheromone a-factor by alpha cells // Nature. - 1983. –Vol. 305.- № 5934. –Р.543-5.
9 Taipakova S.M., Smailov B.B., Stanbekova G.E, Bissenbaev A.K. Cloning and expression of Lentinula edodes cellobiohydrolase gene in E. Coli and characterization of recombinant enzyme // Journal of Cell and Molecular biology. -2011. – Vol. 9. - №1. –P.53-63.
10 Бисенбаев А.К. Тайпакова С.М. Клонирование и экспрессия кДНК целлобиогидролазы СEL7A гриба Lentinula edodes в про- и эукариотических системах и изучение физико-химических свойств рекомбинантного
фермента // Известия НАН РК. Серия биологическая и медицинская. - 2012. - №3(291). - С. 39-46.
How to Cite
Taipakova, S. M., Smekenov, I. T., & Bissenbaev, A. K. (2015). Construction of recombinant Saccharomyces cerevisiae strains containing cellobiohydrolase gene of Lentinula edodes into the chromosomal HO locus. Experimental Biology, 60(2), 352–355. Retrieved from https://bb.kaznu.kz/index.php/biology/article/view/971
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