Изучение физиолого-биохимических характеристик чистых культур молочнокислых бактерий. Сүт қышқылды бактериялардың таза культурасының физиолого-биохимиялық қасиеттерін зерттеу
Ключевые слова:
Молочнокислые бактерии, пептидогликангидролазы, субстратная специфичность, Сүт қышқылды бактериялар, пептидогликангидролазалар, субстрат ерекшелігі, протеолиз,Аннотация
Молочнокислые бактерии синтезируют пептидогликангидролазы (PG гидролазы), которые способны расщеплять ковалентные связи собственных PG и играют важную роль в моделировании PG, необходимом для роста бактерий и деления. Целью настоящей работы было изучение физиолого-биохимических характеристик чистых культур молочнокислых бактерий, выделенных из продуктов питания и микрофлоры организма клинически здорового человека, на примере основных PG гидролаз методом ренатурации в полиакриламидном геле. Бактериялар, PG өзіндік ковалентті байланыстарын ажырататын, бактерияның өсуіне жəне бөлінуіне қажетті PG модельдеуінде негізгі рөл атқаратын пептидогликангидролазаларын (PG гидролазалар) синтездейді.Бұл жұмыстың мақсаты полиакриламидті гелінде ренатурация əдісімен клиникалық сау адам организміндегі микрофлорарадан жəне тағам өнімдерінен сүтқышқылды бактериялардың таза культураларының физиолого-биохимиялық қасиеттерін зерттеуБиблиографические ссылки
1 Vollmer W., Blanot D., de Pedro M.A. Peptidoglycan structure and architecture // FEMS Microbiol Rev. - 2008. – V 32. – P. 149–167.
2 Vollmer W., Joris B., Charlier P., Foster S. Bacterial peptidoglycan (murein) hydrolases // FEMS Microbiol Rev. - .2008. – V. 32. – P. 259–286.
3 Donovan D.M. Bacteriophage and peptidoglycan degrading enzymes with antimicrobial applications // Recent PatBiotechnol. – 2007/ - V.1. – P. 113-122.
4 Regulski, K., Courtin, P., Meyrand, M., Claes, I. J., Lebeer, S., Vanderleyden, J., Hols, P., Guillot, A., and Chapot-
Chartier, M. P. Analysis of the peptidoglycan hydrolase complement of Lactobacillus casei and characterization of the
major gamma-D-glutamyl-L-lysyl-endopeptidase // PLoS One 7, e32301. - 2012.
5 Corthesy B., Gaskins H.R., Mercenier A. Cross-talk between probiotic bacteria and the host immune system // J Nutr.– 2007. - V. 137. - P. 781S–790S
6 Kairova M. and Moldagulova A. Isolation and molecular identification of lactic acid bacteria from horsemeat and
homemade sour cream // J. World academy of Science, Engineering and Technology.Dubai. - 2013. – № 73. - P. 1220-1225
7 Lepeuple A.S., Van Gemert E., Chapot-Chartier M.P. Analysis of the bacteriolytic enzymes of the autolytic
Lactococcus lactis subsp. cremoris strain AM2 by renaturing polyacrylamide gel electrophoresis: identification of a
prophage encoded enzyme // Appl Environ Microbiol. - 1998. – V. – 64. – P. 4142–4148.
8 Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4 // Nature
(London). - 1970. – V. 227. – P. 680–685.
9 Claes I., Schoofs G., Regulski K., Courtin P., Chapot-Chartier M.-P., et al. Genetic and biochemical characterization
of the cell wall hydrolase activity of the major secreted protein of Lactobacillus rhamnosus GG // Plos One;doi. – 2012.- 10.1371/ journal.pone.0031588.
10 O.J. Kang, S. Laberge, R.E. Simard. Detection and Localization of a Peptidoglycan Hydrolase in Lactobacillus
delbrueckiisubsp. Bulgaricus // Journal of Dairy Science. - 2003. – V. 86. – P. 96–104
11 Lebeer S., Vanderleyden J., De Keersmaecker S. Genes and molecules of Lactobacillus supporting probiotic action //
Microbiol Mol Biol Rev. - 2008. – V. 72. – P. 728-764.
12 G. Buist, G. Venema, and J. Kok. Autolysis of Lactococcus lactis Is Influenced by Proteolysis. // Journal of
Bacteriology. – 1998. - 5947–5953 Vol. 180, No. 22
13 Yan F., Polk D.B. Probiotic bacterium prevents cytokine-induced apoptosis in intestinal epithelial cells. // Journal of
Biological Chemistry. – 2002. – V. 277. – P. 50959–50965.
14 Bäuerl C., Pérez-Martínez G., Yan F., Polk D.B., Monedero V. Functional analysis of the p40 and p75 proteins from
Lactobacillus casei BL23 // Journal of Molecular Microbiology and Biotechnology. – 2010. – V.19. – P.231–241.
2 Vollmer W., Joris B., Charlier P., Foster S. Bacterial peptidoglycan (murein) hydrolases // FEMS Microbiol Rev. - .2008. – V. 32. – P. 259–286.
3 Donovan D.M. Bacteriophage and peptidoglycan degrading enzymes with antimicrobial applications // Recent PatBiotechnol. – 2007/ - V.1. – P. 113-122.
4 Regulski, K., Courtin, P., Meyrand, M., Claes, I. J., Lebeer, S., Vanderleyden, J., Hols, P., Guillot, A., and Chapot-
Chartier, M. P. Analysis of the peptidoglycan hydrolase complement of Lactobacillus casei and characterization of the
major gamma-D-glutamyl-L-lysyl-endopeptidase // PLoS One 7, e32301. - 2012.
5 Corthesy B., Gaskins H.R., Mercenier A. Cross-talk between probiotic bacteria and the host immune system // J Nutr.– 2007. - V. 137. - P. 781S–790S
6 Kairova M. and Moldagulova A. Isolation and molecular identification of lactic acid bacteria from horsemeat and
homemade sour cream // J. World academy of Science, Engineering and Technology.Dubai. - 2013. – № 73. - P. 1220-1225
7 Lepeuple A.S., Van Gemert E., Chapot-Chartier M.P. Analysis of the bacteriolytic enzymes of the autolytic
Lactococcus lactis subsp. cremoris strain AM2 by renaturing polyacrylamide gel electrophoresis: identification of a
prophage encoded enzyme // Appl Environ Microbiol. - 1998. – V. – 64. – P. 4142–4148.
8 Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4 // Nature
(London). - 1970. – V. 227. – P. 680–685.
9 Claes I., Schoofs G., Regulski K., Courtin P., Chapot-Chartier M.-P., et al. Genetic and biochemical characterization
of the cell wall hydrolase activity of the major secreted protein of Lactobacillus rhamnosus GG // Plos One;doi. – 2012.- 10.1371/ journal.pone.0031588.
10 O.J. Kang, S. Laberge, R.E. Simard. Detection and Localization of a Peptidoglycan Hydrolase in Lactobacillus
delbrueckiisubsp. Bulgaricus // Journal of Dairy Science. - 2003. – V. 86. – P. 96–104
11 Lebeer S., Vanderleyden J., De Keersmaecker S. Genes and molecules of Lactobacillus supporting probiotic action //
Microbiol Mol Biol Rev. - 2008. – V. 72. – P. 728-764.
12 G. Buist, G. Venema, and J. Kok. Autolysis of Lactococcus lactis Is Influenced by Proteolysis. // Journal of
Bacteriology. – 1998. - 5947–5953 Vol. 180, No. 22
13 Yan F., Polk D.B. Probiotic bacterium prevents cytokine-induced apoptosis in intestinal epithelial cells. // Journal of
Biological Chemistry. – 2002. – V. 277. – P. 50959–50965.
14 Bäuerl C., Pérez-Martínez G., Yan F., Polk D.B., Monedero V. Functional analysis of the p40 and p75 proteins from
Lactobacillus casei BL23 // Journal of Molecular Microbiology and Biotechnology. – 2010. – V.19. – P.231–241.
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Физико-химическая биология и нанотехнологии
