Study of physiological and biochemical characteristics of pure cultures of lactic acid bacteria
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Keywords:
Lactic acid bacteria, peptidoglycan hydrolases, substrate specificity, proteolysis, method of renaturing polyacrylamide gel electrophoresisAbstract
Bacteria synthesize PG hydrolases which are capable of cleaving the covalent bonds of own PG and play an important role in modeling PG, required for bacterial growth and division. The aim of the present work was to study the physiological and biochemical characteristics of pure cultures of lactic acid bacteria isolated from food and microflora of clinically healthy person, on an example of the main PG hydrolases by.References
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Bacteriology. – 1998. - 5947–5953 Vol. 180, No. 22
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Lactobacillus casei BL23 // Journal of Molecular Microbiology and Biotechnology. – 2010. – V.19. – P.231–241.
2 Vollmer W., Joris B., Charlier P., Foster S. Bacterial peptidoglycan (murein) hydrolases // FEMS Microbiol Rev. - .2008. – V. 32. – P. 259–286.
3 Donovan D.M. Bacteriophage and peptidoglycan degrading enzymes with antimicrobial applications // Recent PatBiotechnol. – 2007/ - V.1. – P. 113-122.
4 Regulski, K., Courtin, P., Meyrand, M., Claes, I. J., Lebeer, S., Vanderleyden, J., Hols, P., Guillot, A., and Chapot-
Chartier, M. P. Analysis of the peptidoglycan hydrolase complement of Lactobacillus casei and characterization of the
major gamma-D-glutamyl-L-lysyl-endopeptidase // PLoS One 7, e32301. - 2012.
5 Corthesy B., Gaskins H.R., Mercenier A. Cross-talk between probiotic bacteria and the host immune system // J Nutr.– 2007. - V. 137. - P. 781S–790S
6 Kairova M. and Moldagulova A. Isolation and molecular identification of lactic acid bacteria from horsemeat and
homemade sour cream // J. World academy of Science, Engineering and Technology.Dubai. - 2013. – № 73. - P. 1220-1225
7 Lepeuple A.S., Van Gemert E., Chapot-Chartier M.P. Analysis of the bacteriolytic enzymes of the autolytic
Lactococcus lactis subsp. cremoris strain AM2 by renaturing polyacrylamide gel electrophoresis: identification of a
prophage encoded enzyme // Appl Environ Microbiol. - 1998. – V. – 64. – P. 4142–4148.
8 Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4 // Nature
(London). - 1970. – V. 227. – P. 680–685.
9 Claes I., Schoofs G., Regulski K., Courtin P., Chapot-Chartier M.-P., et al. Genetic and biochemical characterization
of the cell wall hydrolase activity of the major secreted protein of Lactobacillus rhamnosus GG // Plos One;doi. – 2012.- 10.1371/ journal.pone.0031588.
10 O.J. Kang, S. Laberge, R.E. Simard. Detection and Localization of a Peptidoglycan Hydrolase in Lactobacillus
delbrueckiisubsp. Bulgaricus // Journal of Dairy Science. - 2003. – V. 86. – P. 96–104
11 Lebeer S., Vanderleyden J., De Keersmaecker S. Genes and molecules of Lactobacillus supporting probiotic action //
Microbiol Mol Biol Rev. - 2008. – V. 72. – P. 728-764.
12 G. Buist, G. Venema, and J. Kok. Autolysis of Lactococcus lactis Is Influenced by Proteolysis. // Journal of
Bacteriology. – 1998. - 5947–5953 Vol. 180, No. 22
13 Yan F., Polk D.B. Probiotic bacterium prevents cytokine-induced apoptosis in intestinal epithelial cells. // Journal of
Biological Chemistry. – 2002. – V. 277. – P. 50959–50965.
14 Bäuerl C., Pérez-Martínez G., Yan F., Polk D.B., Monedero V. Functional analysis of the p40 and p75 proteins from
Lactobacillus casei BL23 // Journal of Molecular Microbiology and Biotechnology. – 2010. – V.19. – P.231–241.
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Shaikhin, S. M., Moldagulova, A. K., Kazhybaev, A. K., & Bekenova, E. E. (2015). Study of physiological and biochemical characteristics of pure cultures of lactic acid bacteria. Experimental Biology, 59(3/1), 291–301. Retrieved from https://bb.kaznu.kz/index.php/biology/article/view/783
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Physical and chemical biology and nanotechnologies
