СВЯЗЫВАНИЕ МЕЖГЕННЫХ microRNA ЧЕЛОВЕКА С САЙТАМИ mRNA ГЕНОВ, УЧАСТВУЮЩИХ В РАЗВИТИИ РАКА ТОЛСТОЙ КИШКИ
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Keywords:
miRNA, cancer,Abstract
Binding sites of 784 intergenic miRNAs with mRNAs of 54 genes involving in colorectal cancer development were established. Significant difference of miRNA binding sites between 5’UTR, CDS and 3'UTR of mRNA was revealed. Average density of sites for 5'UTR of 40 genes is 33,1 s/l and it is 6,5, 4,7 and 3,8 times more than in 3'UTR, CDS and whole mRNA respectively. Many of intergenic miRNA bind to several mRNA in some sites. For 40 target genes of ig-miRNAs length ratio was, on the average, 4,4%, 59,9% and 29,4% for 5'UTR, CDS and 3'UTR respectively, the proportion of sites interacting with ig-miRNA was 22,4%, 49,2% and 28,4% for with 5'UTR, CDS and 3'UTR respectively. It follows from our research results that miRNAs might regulate gene expression by interaction to 3'UTR and affect on 5'UTR and CDS too.References
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6. Bracken C.P., Szubert J.M., Mercer T.R., Dinger M.E. et al.. Global analysis of the mammalian RNA degradome reveals widespread miRNA-dependent and miRNA-independent endonucleolytic cleavage // Nucleic Acids Research.- 2011.- V.39.- P.5658–5668.
7. Orom U.A., Nielsen F.C., Lund A.H. MicroRNA-10a binds the 5’UTR of ribosomal protein mRNAs and enhances their translation // Molecular Cell.- 2008.- V.30.- P.460-471.
8. Tsai N.P., Lin Y.L., Wei L.N. MicroRNA mir-346 targets the 5'-untranslated region of receptor-interacting protein 140 (RIP140) mRNA and up-regulates its protein expression // Biochem J..- 2009.- V.424.- P.411-418.
9. Moretti F., Thermann R., Hentze M.W. Mechanism of translational regulation by miR-2 from sites in the 5′ untranslated region or the open reading frame // RNA.- 2010.- V.16.- P.2493-25.
10. Ajay S.S., Athey B.D., Lee I. Unified translation repression mechanism for microRNAs and upstream AUGs // BMC Genomics.- 2010.- V.11.- P. 155-165.
11. Ying S.Y., Chang C.P., Lin S.L. Intron-mediated RNA interference, intronic microRNAs, and applications // Methods Mol Biol.- 2010.- V.629.- P.205-237.
12. Feber A., Xi L., Pennathur A., Gooding W.E., Bandla S. et al. MicroRNA prognostic signature for nodal metastases and survival in esophageal adenocarcinoma // Ann. Thorac. Surg..- 2011.- V.91.- P.1523-1530.
13. Gao C., Zhang Z., Liu W., Xiao S., Gu W., Lu H Reduced microRNA-218 expression is Associated with high nuclear factor kappa B activation in gastric cancer // Cancer.- 2010.- V.116.- P.41-49.
14. Li X., Zhang Y., Zhang H., Liu X. miRNA-223 promotes gastric cancer invasion and metastasis by targeting tumor suppressor EPB41L3 // Mol Cancer Res..- 2011.- V.9.- P.824–833.
15. Zhang J., Guo H., Zhang H., Wang H., Qian G. et al. Putative tumor suppressor miR-145 inhibits colon cancer cell growth by targeting oncogene friend leukemia virus integration 1 gene // Cancer.- 2011.- Vol.117.- No.1.- P.86-95.
16. Bishop J.A., Benjamin H., Cholakh H., Chajut A., Clark D.P., Westra W.H. Accurate classification of non–small cell lung carcinoma using a novel MicroRNA-based approach // Clin. Cancer Res..- 2010.- V.16.- Р.610-619.
17. Galardi S., Mercatelli N., Farace M.G., Ciafre S.A. NF-kB and c-Jun induce the expression of the oncogenic miR-221 and miR-222 in prostate carcinoma and glioblastoma cells // Nucleic Acids Research.- 2011.- V.39.- Р.3892–3902.
18. Fu S.W., Chen L., Man Y.-G.. miRNA biomarkers in breast cancer detection and management // J. Cancer.- 2011.- V.2.-Р.116-122.
19. Zhang Y., Li M., Wang H. Profiling of 95 microRNAs in pancreatic cancer cell lines and surgical specimens by real time PCR analysis // World J. Surg..- 2009.- V.33.- Р.698–709.
20. Corney D.C., Nikitin A.Y. MicroRNA and ovarian cancer // Histol Histopathol..- 2008.- V.23.- Р.1161–1169.
21. Rachagani S., Kumar S., Batra S.K. MicroRNA in pancreatic cancer: pathological, diagnostic and therapeutic implications // Cancer Letters.- 2010.- V.292.- P.8-16.
22. Moussay E., Wang K., Cho J.-H., van Moer K., Pierson S., Paggetti J. et al. MicroRNA as biomarkers and regulators in Bcell chronic lymphocytic leukemia // PNAS.- 2011.- V.108.- No. 16., P. 6573-6578.
23. Hu Y., Correa A.M., Hoque A. et al.. Prognostic significance of differentially expressed miRNAs in esophageal cancer // Intern. J. Cancer.- 2010.- V.128.- No.1.- P.132-43.
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Исабекова, А. С., Хайленко, В. А., & Иващенко, А. Т. (2017). СВЯЗЫВАНИЕ МЕЖГЕННЫХ microRNA ЧЕЛОВЕКА С САЙТАМИ mRNA ГЕНОВ, УЧАСТВУЮЩИХ В РАЗВИТИИ РАКА ТОЛСТОЙ КИШКИ. Experimental Biology, 56(4), 307–312. Retrieved from https://bb.kaznu.kz/index.php/biology/article/view/736
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