САЙТЫ СВЯЗЫВАНИЯ ИНТРОННЫХ microRNA С 5'UTR, CDS И 3'UTR mRNA ГЕНОВ, УЧАСТВУЮЩИХ В РАЗВИТИИ РАКА ТОЛСТОЙ КИШКИ ЧЕЛОВЕКА

Авторы

  • О. А. Берилло Казахский национальный университет имени аль-Фараби
  • В. А. Хайленко Казахский национальный университет имени аль-Фараби
  • А. Т. Иващенко Казахский национальный университет имени аль-Фараби

Ключевые слова:

гибридизация, miRNA, mRNA, ісік ауруы, гетерогенділік

Аннотация

Установлены сайты гибридизации 686 интронных miRNA с mRNA 54 генов, которые принимают участие в развитии рака толстой кишки человека. Выявлено разное соотношение сайтов связывания изученных miRNA с 5'UTR, CDS и 3'UTR mRNA каждого гена. Установлена значительная гетерогенность функциональных участков mRNA по числу сайтов связывания и по плотности расположения этих сайтов с miRNA. Найдены miRNA, обладающие высокой селективностью к mRNA некоторых генов. Бұл жұмыста 686 интронды miRNA-дың адамның тоқ ішек ісік ауруының дамуына қатысатын 54 геннің mRNA-мен гибридизация сайттары анықталды. Зерттелген miRNA-дың əрбір ген mRNA-ның 5'UTR, CDS жəне 3'UTR-мен байланыс сайттарының əртүрлі арақатынасы айқындалған. RNA функциональды бөліктерінің miRNA- мен байланыстыру сайттарының орналасу тығыздығына байланысты жəне бұл сайттардың саны бойынша гетерогенділік анықталды. Кейбір miRNA гендердің mRNA-на сұрыптаушылықпен ерекшеленеді.

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БИОТЕХНОЛОГИЯ, БИОХИМИЯ И ФИЗИОЛОГИЯ РАСТЕНИЙ

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