Multiple binding sites miRNA with mRNA of human genes
Keywords:
miRNA, mRNA, nucleotides, binding sites, target genes,Abstract
We searched the structural organization of binding sites miR-3960, miR-3620-5p and miR-8072 in the mRNA of human genes. Identified sites mRNA in which through 1-5 nucleotides several binding sites of miR-3960 are located. The nucleotide sequences of these sites in the CDS encode polyalanine or polyproline. In 5’UTR and 3’UTR structural organization of miR binding sites identical to that in the CDS. Shown that miR-3960, miR-3620-5p and miR-8072 can bind with genes involved in cell cycle regulation and apoptosis. Discusses the properties of miR-3960, miR-3620-5p and miR-8072 and their role in the development of cancer.References
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2 Ivashchenko A.T., Issabekova AS, Berillo O.A. miR-1279, miR-548j, miR-548m, and miR-548d-5p Binding Sites in CDSs of Paralogous and Orthologous PTPN12, MSH6, and ZEB1 Genes // Biomed Res Int.: Mol.Biol. – 2013. – Vol. 2013. – P. 1-10.
3 Berillo O., Régnier M., Ivashchenko A. Binding of intronic miRNAs with mRNAs of genes coding intronic microRNAs and proteins participating in tumourigenesis // Computers in Biology and Medicine. – 2013. – Vol.43. – Р. 1374-1381.
4 Kool E.T. Hydrogen bonding, base stacking, and steric effects in DNA replication // Annu Rev Biophys Biomol Struct. – 2001.– Vol.30. – P. 22.
5 Leontis N.B., Stombaugh J., Westhof E. The non-Watson-Crick base pairs and their associated isostericity matrices // Nucleic Acids Res. – 2002. – Vol. 30. – P. 3497–3531.
6 Ниязова Р.Е., Иващенко А.Т., Берилло О.А. и др. Особенности сайтов связывания miR-3960, miR-3620-5p и miR-8072 с mRNA генов человека // Вестник КазНУ серия биологическая. – 2013. – T. 3/1, № 59. – С. 281-285.
7 Hu R. A Runx2/miR-3960/miR-2861 regulatory feedback loop during mouse osteoblast differentiation // J Biol Chem. – 2011.– Vol. 286. – P. 12328–12339.
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