Cloning of lactobacilli peptidoglycan hydrolases genes
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Keywords:
peptidoglycan hydrolase, cloning, lactobacilli,Abstract
Peptidoglycanhydrolases play an important role in Lactobacilli cells division and at the same time, have a significant effect on the immune status of the colonized organisms. Genes encoding peptidoglycanhydrolases of Lactobacillus rhamnosus GG p40 and p75 and their homologues are the objects of interest for cloning to enhance their expression in the homo- and heterologous background. The sequences of p40 and p75 homologues from strains Lactobacillus rhamnosus BSR and Lactobacillus casei BI005 were found using specific primers, and products of their amplification were cloned into the pGEM-T vector. The resulting plasmid constructions will be used for further manipulations to study the peptidoglycanhydrolases genes expression in E.coli cells.References
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2 Lebeer S., Vanderleyden J., De Keersmaecker S.C.J. Genes and molecules of lactobacilli supporting probiotic action // Microbiology and Molecular Biology Reviews. – 2008. – V 72. – Issue 4. – P. 728–764.
3 Bäuerl C., Pérez-Martínez G., Yan F., Polk D.B., Monedero V. Functional analysis of the p40 and p75 proteins from Lactobacillus casei BL23 // Journal of Molecular Microbiology and Biotechnology. – 2010. – V.19. – P.231–241.
4 Kibbe W.A. OligoCalc: an online oligonucleotide properties calculator // Nucl. Acids Res.– 2007. – Vol. 35. –
5 Claes I.J.J, Schoofs G., Regulski K., Courtin P., Chapot-Chartier M., Rolain P., Hols P., von Ossowski I., Reunanen J., de Vos W.V., Palva A., Vanderleyden J., De Keersmaecker S.C.J., Lebeer S. Genetic and biochemical characterization of the cell wall hydrolase activity of the major secreted protein of Lactobacillus rhamnosus GG // PLoS One. – 2012. – V. 7. – Issue 2. – e31588.
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7 Green M., Sambrook J. Molecular cloning. A laboratory manual // Cold Spring Harbor Laboratory Press. – 2012. – P. 2028.
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Li, K. G., Moldagulova, A. K., Bekenova, E. E., Kazybaev, A. K., Urazova, M. S., Shajhin, S. M., & Almagambetov, K. H. (2015). Cloning of lactobacilli peptidoglycan hydrolases genes. Experimental Biology, 60(1), 38–41. Retrieved from https://bb.kaznu.kz/index.php/biology/article/view/52
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Bioinformatics, Genomics and Proteomics. Physical-chemical Biology
