Associations of miRNAs with mRNAs of genes participating in the development of non-small cell lung cancer
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Keywords:
miRNA, mRNA, gene, non-small cell lung cancer, subtypes.Abstract
The binding of miRNAs with mRNAs of genes involved in the development of various subtypes of human lung cancer was studied. A computer program predicted miRNAs binding sites with genes mRNAs with high reliability was developed. The program calculates the ratio of ΔG/ΔGm, the reliability value, the location of the microRNA binding site in the 5'-untranslated region (5'UTR), in the protein coding portion (CDS) or in the 3'untranslated region (3'UTR). A search of miRNAs target genes was calculated with the MirTarget program. It was created a database of genes involved in the development of lung squamous cell carcinoma and large cell carcinoma. It were identified miRNAs which effectively bind to mRNAs of target gene. It were established associations of specific miRNAs with mRNAs of genes increased and reduced-expressed in squamous cell lung cancer. Among the miRNAs involved in the associations with mRNAs, the largest number of target genes was found for unique miRNAs: miR-1273g-3p, miR-1273h-5p, miR-1273f, miR-1322, miR-1285-5p, miR-619-5p. The obtained results of the effect of miRNAs on the expression of genes involved in oncogenesis may serve as a basis for the development of early diagnosis methods of lung cancer subtypes.References
Литература
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1) Travis W., Brambilla E., Mueller-Hermelink H., Harris C. World health organization classification of tumors. Pathology and genetics: tumors of the lung, pleura, thymus and heart // World health organization. - Geneva, 2004. - P. 341.
2) Тажединов И. Cовременные аспекты развития ядерной медицины в онкологии Казахстана // Общие вопросы диагностики и лечения в онкологии. Материалы Съезда онкологов и радиологов Казахстана. - Алматы, 2014. - 143с.
3) Koyi H., Hillerdal G., Branden E. A prospective study of a total material of lung cancer from a county in Sweden 1997-1999: gender, symptoms, type, stage, and smoking habits // Lung Cancer. - 2002. - Vol. 36. - P. 39.
4) Visbal A.L., Williams B.A., Nichols F.C. et al. Gender differences in non-small-cell lung cancer survival: an analysis of 4,618 patients diagnosed between 1997 and 2002 // The Annals of Thoracic Surgery. - 2004. - Vol. 78. - P. 209–215.
5) Li Q., Li X., Guo Zh., et al. MicroRNA-574-5p was pivotal for TLR9 signaling enhanced tumor progression via down-regulating checkpoint suppressor 1 in human lung cancer // Plos One. - 2012. - Vol. 7. - e48278.
6) Ivashchenko А., Berillo О., Pyrkova A., Niyazova R., Atambayeva S. Binding sites of mir-1273 family on the mRNA of target genes // Biomed Research International. - 2014. - Vol. 2014. - P. 620530.
7) Ivashchenko A., Berillo O., Pyrkova A., Niyazova R., Atambayeva Sh. The properties of binding sites of miR-619-5p, miR-5095, miR-5096 and miR-5585-3p in the mRNAs of human genes // Biomed Research International. - 2014. - Vol. 2014. - P. 720715.
8) Ivashchenko A., Berillo O., Pyrkova A., Niyazova R., Atambayeva Sh. MiR-3960 binding sites with mRNA of human genes // Bioinformation. - 2014. - Vol. 10(7). - P. 423-427.
9) Cheng Q., Yi B., Wang A., Jiang X. Exploring and exploiting the fundamental role of microRNAs in tumor pathogenesis // Onco Targets Ther. - 2013. - Vol. 6. - P. 1675-1684.
10) Guz M., Rivero-Muller A., Okon E., et al. MicroRNAs-role in lung cancer // Disease Markers. - 2014. - Vol. 2014. - P. 218169.
11) Gao Y., Gao F., Ma J.L., et al. The potential clinical applications and prospects of microRNAs in lung cancer // Journal of Onco Targets and Therapy. - 2014. - Vol. 7. - P. 901-906.
12) Waters P.S., Dwyer R.M., Brougham C., et al. Impact of tumour epithelial subtype on circulating microRNAs in breast cancer patients // Plos one. - 2014. - Vol. 9(3). - e90605.
13) Zhang X., Wei J., Zhou L., et al. A functional BRCA1 coding sequence genetic variant contributes to risk of esophageal squamous cell carcinoma // Carcinogenesis. - 2013. - Vol. 34(10). - P. 2309-2313.
14) Chan W.L., Yuo C.Y., Yang W.K., Hung S.Y., Chang Y.S., Chiu C.C., Yeh K.T., Huang H.D., Chang J.G. Transcribed pseudogene ψPPM1K generates endogenous siRNA to suppress oncogenic cell growth in hepatocellular carcinoma // Nucleic Acids Research. - 2013. - Vol. 41(6). - P. 3734-3747.
15) Li X., Chen Y.T., Josson S., Mukhopadhyay N.K., Kim J., Freeman M.R., et al. MicroRNA-185 and 342 inhibit tumorigenicity and induce apoptosis through blockade of the SREBP metabolic pathway in prostate cancer cells // PLoS One. - 2013. - Vol. 8. - No. 8.- e70987.
16) He L., Thomson J.M., Hemann M.T., Hernando-Monge E., Mu D., Goodson S., Powers S., Cordon-Cardo C., Lowe S.W., Hannon G.J., et al. A microRNA polycistron as a potential human oncogene // Nature. - 2005. - Vol. 435. - P. 828-833.
17) Griffiths-Jones S., Enright A.J., Farazi T.A. et al. MicroRNA research // The 2008 Collection booklet: Exiqon. - 2008. - P. 36.
18) Berillo O., Regnier M., Ivashchenko A.T. miRAFinder and GeneAFinder scripts: large-scale searching for microRNA and related information in indexed literature abstracts // Bioinformation. - 2014. - Vol. 10(8). - P. 539-543.
19) Berillo O., Regnier M., Ivashchenko A.T. TmiRUSite and TmiROSite scripts: searching for mRNA fragments with microRNA binding sites and their encoded for amino acid sequences // Bioinformation. - 2014. - Vol. 10(7). - P. 472-473.
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How to Cite
Niyazova, R. Y., Atambayeva, S. A., Pyrkova, A. Y., & Ivashchenko, A. T. (2016). Associations of miRNAs with mRNAs of genes participating in the development of non-small cell lung cancer. Experimental Biology, 65(3), 142–148. Retrieved from https://bb.kaznu.kz/index.php/biology/article/view/1110
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МOLECULAR BIOLOGY AND GENETICS
