microRNAs and genes associated with the development of atherosclerosis

Abstract

It was created a database of 212 genes and 39 microRNAs involved in the development of atherosclerosis. Only miR-103a-3p has binding site in mRNA of ADAMTS7 gene with the value of ΔG/ΔGm more than 90%. 39 miRNAs have 118 binding sites in the mRNA genes with the value of ΔG/ΔGm from 90 to 100%. IRS2 gene mRNA contains 17 binding sites for miRNAs. mRNA of LMNA, MTHFR, F11R, LDLR genes have a number of miRNAs binding sites equal to 14, 11, 10 and 9, respectively. miR-619-5p and miR-5096 have completely complementary binding sites in the mRNA of ADAM17 and IL18 genes. miR-619-5p has binding sites in mRNAs of 11 genes. miR-466 has multiple binding sites in the mRNA of five genes. miR-1322 has multiple binding sites in the mRNA of PDE4D, RTN3 genes, and miR-574-5p interacted with multiple sites in the mRNA PPARA gene. miRNAs binding sites in the mRNA of IL18, IL10, LDLR, BRCA1, F11R genes have a value of ΔG/ΔGm equal to 98 - 100%. mRNAs of CDKN1C, SIRT1, TGFB1, APH1B genes contain binding sites with the interaction energy of 127 - 136 kJ/mole. mRNA of CDKN1C gene contains 35 binding sites for miR-762 in CDS.

It was created a database of 212 genes and 39 microRNAs involved in the development of atherosclerosis. Only miR-103a-3p has binding site in a protein-coding region of ADAMTS7 gene mRNA in with the value of ΔG/ΔGm more than 90%. 39 miRNAs involved in atherosclerosis have 118 binding sites with the value of ΔG/ΔGm from 90 to 100%. miR-145-3p and miR-663a have eight target genes with the value of ΔG/ΔGm equal to 91-92%. It was revealed 49 target genes associated with the development of atherosclerosis, which have one miRNA binding sites with the ΔG/ΔGm value more than 90%. Ten sites are located in the 5'UTRs, 20 sites - in the CDSs and 19 sites - in the 3'UTRs. miR-1273g-3p with mRNA of ALOX15 gene (98%), miR-3653 with the mRNA of HMGB1 gene (98%) and miR-1273g-3p with mRNA of TFPI gene (98%) have the maximum ΔG/ΔGm value. 93 target genes mRNAs have multiple sites for miRNAs with the ΔG/ΔGm value not less than 90%. mRNA of IRS2 gene contain binding sites of miR-1227-5p, miR-4466, miR-1268a, miR-1268b, miR-3913-5p, miR-4488, miR-1181, miR-4258, miR-3960, miR-7977, miR-5703, miR-4468, miR-7704, miR-4279, miR-6806-5p, miR-6071, miR-3665 miRNAs with the value of ΔG/ΔGm from 90 to 96%, which indicated the strong dependence of gene expression from miRNAs. mRNA of LMNA, MTHFR, F11R, LDLR genes have a number of miRNAs binding sites equal to 14, 11, 10 and 9, respectively. miR-619-5p and miR-5096 have completely complementary binding sites (the value of ΔG/ΔGm equal to 100%) in ADAM17 and IL18 genes mRNAs, respectively. The unique miR-619-5p has binding sites in the mRNAs of TNFSF10, PPARA, PNPLA3, MTHFR, LDLR, ITGA2, IL18, IL10, BRCA1, ADAM33, ADAM17 genes. The unique miR-5096 has binding sites in the mRNAs of TNC, PPARA, ITGA2, IL18, IL10, BRCA1, ANGPT2 genes. The unique miR-1273g-3p bind only with mRNA of NLRP3, ICAM genes. miR-466 has multiple binding sites in the mRNA of TNFSF4, PPARGC1A, PLA2G7, NOS1AP, ICAM1 genes. miR-1322 has multiple binding sites in the mRNA of PDE4D, RTN3 genes, and miR-574-5p bind with multiple sites in the PPARA gene mRNA. miRNAs binding sites in the mRNA IL18, IL10, LDLR, BRCA1, F11R genes have the highest ΔG/ΔGm value equal to 98 - 100%. mRNA of CDKN1C, SIRT1, TGFB1, APH1B genes contain binding sites with high interaction energy equal to 127 - 136 kJ/mole. CDKN1C gene mRNA contains 35 binding sites in the CDS, which certainly makes a special interest in this association mRNA and miR-762. The role of miRNAs and genes in the development of atherosclerosis was studied.