microRNAs and genes associated with metabolic syndrome

Abstract

It was created the database of genes and miRNAs, involved in the development of metabolic syndrome. 118 mRNA of genes bind with 94 miRNAs with the value of ΔG/ΔGm more than 90%. For 48 miRNAs binding sites are located in the CDS, 19 miRNA - 5'UTR, 43 miRNA - 3'UTR. Some miRNAs have polysites: miR-466 - four in mRNA of the CD36 gene, nine mRNA in mRNA of JAK2 gene, miR-619-5p - three mRNA of LDLR gene, miR-3960 - three mRNA CEBPA gene. miR-3960 binds to mRNA of four target genes, miR-466 - six, miR-1273f, miR-5095 - seven, miR-1285-5p - eight, miR-5096 - nine, miR-5585-3p - ten, miR -1273g-3p, miR-619-5p - 13 target genes. Of the 42 miRNA, the expression of 33 miRNA rises and nine miRNA decreases in metabolic syndrome. Some miRNAs have a large number of target genes: miR-185-3p, miR-378g have 11 target genes, miR-197-3p, miR-378d - ten, miR-150-3p, miR-378a-3p, miR- 378i - nine target genes, miR-145-3p - eight, miR-197-5p, miR-378b - seven target genes.

It was created the database of genes and miRNAs, involved in the development of metabolic syndrome. 118 mRNA of genes bind with 94 miRNAs with the value of ΔG/ΔGm more than 90%. For 48 miRNAs binding sites are located in the CDS, 19 miRNA - in the 5'UTR, 43 miRNA - in the 3'UTR. Some miRNAs have polysites: miR-466 - four sites with mRNA of the CD36 gene, nine sites with mRNA of JAK2 gene, miR-619-5p - three sites with mRNA of LDLR gene, miR-3960 - three sites with mRNA of CEBPA gene. Some miRNAs have multiple target genes involved in the development of metabolic syndrome. miR-3960 binds to mRNA of four target genes, miR-466 – six genes, miR-1273f, miR-5095 – seven genes, miR-1285-5p – eight genes, miR-5096 – nine genes, miR-5585-3p – ten genes, miR -1273g-3p, miR-619-5p - 13 target genes. Of the 42 miRNA, participating in the development of metabolic syndrome, the expression of 33 miRNA rises and nine miRNA decreases in metabolic syndrome. Some miRNAs have a large number of target genes: miR-185-3p, miR-378g have 11 target genes, miR-197-3p, miR-378d – ten genes, miR-150-3p, miR-378a-3p, miR- 378i – nine genes, miR-145-3p – eight genes, miR-197-5p, miR-378b - seven target genes. Some miRNA have a greater free energy of binding with mRNA of several genes. miR-378a-5p consists of 22 nt. It binds to mRNA of COL7A1 gene with the free binding energy equal to -119 kJ/mole, which is 98% of the maximum free binding energy. miR-197-5p consist of 23 nt. It binds with mRNA FAM212B, FMN2, C22orf46, PRDM1, ZFP36L1, MAP3K10, TNRC18 genes with the free binding energy equal to -119 kJ/mole, which is 90% of the maximum binding free energy. The miR-378 family have binding sites in the 3'UTR, 5'UTR and CDS of mRNAs of target genes. miR-378a-3p with a length of 21nt have 9 binding sites in mRNA of target genes, six sites of which are located in the 3'UTR, two sites are located in the CDS, one site is located in the 5'UTR. miR-378a-3p has a length of 22 nt and binds to mRNA of four target genes, three of which are located in the CDS, one is located in the 3'UTR. miR-378b with a length of 19 nt has seven target genes, miR-378d – ten genes, miR-378e – six genes, miR-378f – five genes, miR-378g – 11 genes, miR-378h – three genes, miR-378i – nine genes, miR-378j - two target genes.