Тәжірибелік созылмалы қабыну кезіндегі миелоидық супрессорлық жасушалар
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Кілттік сөздер:
Созылмалы қабыну, MDSC, ағынды цитометрия, эрлих ісігі, адъювант артритАннотация
Миелоидты супрессорлық жасушалар (MDSC) тышқандарда CD11b және Gr-1 жасушалық маркерлерінің экспрессиясы бойынша анықталған, цитотоксикалық Т-лимфоциттер мен NK-жасушалардың ісікке қарсы белсенділіктерін төмендетуге қабілетті, миеломоноцитарлық бағыттағы ( нейтрофилдер және моноциттер) гемопоэтикалық бастапқы өзіндік гетерогенді популяция. Біз MDSC феноменін ісік процессінде бірегей болмайды, қайта оның алдында болатын және онкогенезді белгілі деңгейде белсендендіретін созылмалы қабыну патогенезімен байланысты деп жорамалдаймыз. Жұмыстың мақсаты болып созылмалы қабынудың өзіндік классикалық моделі тышқандарындағы адъювант артриттің даму динамикасындағы MDSC субпопуляциясын цитофлуориметриялық зерттеу. Негізгі әдіс ретінде, СВА линиялы тышқандардағы Gr-1+CD11b MDSC деңгейінің жоғарлаумен жақсы арақатынаста болатын тері асты Эрлих ісігінің өсіу моделінде бекітілген MDSC-ді фенотипикалық бағалауға жарамды ағынды цитометрия әдісі қолданылды. Біз толық адъювант Фрейндпен әсер етілген, CD-1 линиялы тышқандардың артқы аяқтарындағы созылмалы қабынудың дамуының 2- аптасының соңына қарай, көкбаурындағы екі MDSC фракцияларының (M және G ) екі еседен көп жоғарлауы болатынын және ол 4-аптаның соңына дейін сақталатынын анықтадық. Бұдан басқа әртүрлі зерттеу мерзімінде CD184, CD195 және CD62L молекулаларын экспрессиялайтын CD11b+Gr-1+ жасушалар үлесі айтарлықтай жоғарлағаны белгілі болды. Бұл қабыну процесіне MDSC қатысатынын және олардың қабыну ошағына осы молекулалар көмегімен жылжитынын көрсетеді.
Библиографиялық сілтемелер
Әдебиеттер
1 Prasad S., Sung B., Aggarwal B.B. Age-associated chronic diseases require age-old medicine: Role of chronic inflammation // Prev Med.- 2012.- N54.– P.29–37. DOI:10.1016/j.ypmed.2011.11.011
2 Yu B.P., Chung H.Y. The inflammatory process in aging // Rev Clin Gerontol.- 2006.- N16.– P.179–187. DOI: 10.1017/S0959259807002110
3 Lavrovsky Y, Chatterjee B, Clark R.A., Roy A.K. Role of redox-regulated transcription factors in inflammation, aging and age-related diseases // Exp Gerontol.- 2000.- N35.– P.521–532. DOI:10.1016/S0531-5565(00)00118-2
4 Rahman I. Oxidative stress, chromatin remodeling and gene transcription in inflammation and chronic lung diseases // J Biochem Mol Biol.- 2003.- N36.– P.95–109. DIO: 10.5483/BMBRep.2003.36.1.095
5 Aggarwal B.B. Nuclear factor-kB: the enemy within // Cancer Cell.- 2004 - N6.– P.203–208. DIO:10.1016/j.ccr.2004.09.003
6 Roy S., Bagchi D., Raychandhuri S.P. Chronic inflammation Molecular pathophysiology, nutritional and therapeutic interventions. – USA: CRC Press, Talor@Francis Group L.L.C.- 2013.- 472 p.
7 Zeyda M., Farmer D., Todoric J., Aszmann O., Speiser M., Györi G., Zlabinger G.J., Stulnig T.M. Human adipose tissue macrophages are of an anti-inflammatory phenotype but capable of excessive pro-inflammatory mediator production // Int J Obes.- 2007.- N31.– P.1420–1428. DIO: 10.1038/sj.ijo.0803632
8 Wolf A.M., Wolf D., Rumpold H., Enrich B., Tilg H. Adiponectin induces the anti-inflammatory cytokines IL-10 and IL-1RA in human leukocytes // Biochem Biophys Res Com.- 2004.- N323.– P.630–635. DIO: 10.1016/j.bbrc.2004.08.145
9 Lan H.Y. Diverse roles of TGF-β/Smads in renal fibrosis and Inflammation// Int J Biol Sci.- 2011.- N7.– P.1056-1067.DIO: 10.7150/ijbs.7.1056
10 Han G., Li F., Singh T.P., Wolf P., Wang X.J. The Pro-inflammatory Role of TGFβ1: A Paradox? // Int J Biol S.- 2012.- N8(2).– P.228-235. DIO: 10.7150/ijbs.8.228
11 Gabrilovich D.I., Nagaraj S. Myeloid-derived suppressor cells as regulators of the immune system // Nature Rev Immunol.- 2009.- N9.– P.162-174. DIO: 10.1038/nri2506
12 Greten T.F., Manns M.P., Korangy F. Myeloid derived suppressor cells in human diseases // Intern Immunopharm.- 2011.- N11.- P.802–807. DIO: 10.1016/j.intimp.2011.01.003
13 Ostrand-Rosenberg S., Sinha P. Myeloid-derived suppressor cells: Linking inflammation and cancer // J Immunol.- 2009.- N182.- P.4499–4506. DOI: 10.4049/jimmunol.0802740
14 Peranzoni E., Zilio S., Marigo I., Dolcetti L., Zanovello P., Mandruzzato S., Bronte V. Myeloid-derived suppressor cell heterogeneity and subset definition // Curr Opin Immunol.- 2010.- N22.- P.238–244. DIO: 10.1016/j.coi.2010.01.021
15 Obregón-Henao A., Henao-Tamayo M., Orme I.M., Ordway D.J. Gr1intCD11b+ Myeloid-derived suppressor cells in Mycobacterium tuberculosis Infection // PLOS ONE.- 2013.- N8.- Issue 11 e80669. DIO: 10.1371/journal.pone.0080669
16 Chen S., Akbar S.M., Abe M., Hiasa Y., Onji M. Immunosuppressive functions of hepatic myeloid-derived suppressor cells of normal mice and in a murine model of chronic hepatitis B virus // Clin Exp Immunol.- 2011.- N166.- P.134–142. DIO: 10.1111/j.1365-2249.2011.04445.x
17 Xia S.h., Sha H., Yang L., Ji Y., Ostrand-Rosenberg S., Qi L. Gr-1 CD11b Myeloid-derived suppressor cells suppress inflammation and promote insulin sensitivity in obesity // J Biol Chem.- 2011.- N286(26).- P.23591–23599. DIO: 10.1074/jbc.M111.237123
18 Goni O., Alcaide P., Fresno M. Immunosuppression during acute Trypanosoma cruzi infection: involvement of Ly6G (Gr1(+)) CD11b(+) immature myeloid suppressor cells // Int Immunol.- 2002.- N14(10).- P.1125-1131. DIO: 10.1093/intimm/dxf076
19 Mencacci A., Montagnoli C., Bacci A., Cenci E., Pitzurra L., Spreca A., Kopf M., Sharpe A.H., Romani L. CD80+Gr-1+ myeloid cells inhibit development of antifungal Th1 immunity in mice with candidiasis // J Immunol.- 2002.- N169.- P.3180-3189. DIO: 10.4049/jimmunol.169.6.3180
20 European Convention for the Protection of Vertebrate Animals used for Experimental and Other Scientific Purposes // CETS 123. Strasbourg.- 1986.- 11 p.
21 Subiza J.L.,Vinuela J.E., Rodriguez R., Gil J., Figueredo M.A., De la Concha E.G. Development of splenic natural suppressor (NS) cells in Ehrlich tumor-bearing mice // Int J Cancer.- 1989.- N44.- P307-314. DIO: 10.1002/ijc.2910440220
References
1 Prasad S, Sung B, Aggarwal BB (2012) Age-associated chronic diseases require age-old medicine: Role of chronic inflammation, Prev Med, 54: S29–S37. DOI:10.1016/j.ypmed.2011.11.011
2 Yu BP, Chung HY (2006) The inflammatory process in aging, Rev Clin Gerontol, 16:. 179–187. DOI: 10.1017/S0959259807002110
3 Lavrovsky Y, Chatterjee B, Clark RA, Roy AK (2000) Role of redox-regulated transcription factors in inflammation, aging and age-related diseases, Exp Gerontol, 35: 521–532. DOI:10.1016/S0531-5565(00)00118-2
4 Rahman I (2003) Oxidative stress, chromatin remodeling and gene transcription in inflammation and chronic lung diseases,J Biochem Mol Biol, 36: 95–109. DIO: 10.5483/BMBRep.2003.36.1.095
5 Aggarwal BB (2004) Nuclear factor-kB: the enemy within, Cancer Cell, 6: 203–208. DIO:10.1016/j.ccr.2004.09.003
6 Roy S, Bagchi D, Raychandhuri SP (2013) Chronic inflammation Molecular pathophysiology, nutritional and therapeutic interventions, CRC Press, Talor@Francis Group L.L.C., USA. ISBN:978-1-4398-7211-6
7 Zeyda M, Farmer D, Todoric J, Aszmann O, Speiser M, Györi G, Zlabinger GJ, Stulnig TM (2007) Human adipose tissue macrophages are of an anti-inflammatory phenotype but capable of excessive pro-inflammatory mediator production,Int J Obes, 31: 1420–1428. DIO: 10.1038/sj.ijo.0803632
8 Wolf AM, Wolf D, Rumpold H, Enrich B, Tilg H (2004) Adiponectin induces the anti-inflammatory cytokines IL-10 and IL-1RA in human leukocytes, Biochem Biophys Res Com, 323: 630–635. DIO: 10.1016/j.bbrc.2004.08.145
9 Lan HY (2011) Diverse roles of TGF-β/Smads in renal fibrosis and Inflammation, Int J Biol Sci, 7: 1056-1067.DIO: 10.7150/ijbs.7.1056
10 Han G, Li F, Singh TP, Wolf P, Wang XJ (2012) The Pro-inflammatory Role of TGFβ1: A Paradox?, Int J Biol S, 8(2):228-235. DIO: 10.7150/ijbs.8.228
11 Gabrilovich DI, Nagaraj S (2009) Myeloid-derived suppressor cells as regulators of the immune system, Nature Rev Immunol, 9:162-174. DIO: 10.1038/nri2506
12 Greten TF, Manns MP, Korangy F (2011) Myeloid derived suppressor cells in human diseases, Intern Immunopharm, 11: 802–807. DIO: 10.1016/j.intimp.2011.01.003
13 Ostrand-Rosenberg S, Sinha P (2009) Myeloid-derived suppressor cells: Linking inflammation and cancer, J Immunol, 182: 4499–4506. DOI: 10.4049/jimmunol.0802740
14 Peranzoni E, Zilio S, Marigo I, Dolcetti L, Zanovello P, Mandruzzato S, Bronte V (2010) Myeloid-derived suppressor cell heterogeneity and subset definition, Curr Opin Immunol, 22: 238–244. DIO: 10.1016/j.coi.2010.01.021
15 Obregón-Henao A, Henao-Tamayo M, Orme IM, Ordway DJ (2013) Gr1intCD11b+ Myeloid-derived suppressor cells in Mycobacterium tuberculosis Infection, PLOS ONE, 8: Issue 11 e80669. DIO: 10.1371/journal.pone.0080669
16 Chen S, Akbar SM, Abe M, Hiasa Y, Onji M (2011) Immunosuppressive functions of hepatic myeloid-derived suppressor cells of normal mice and in a murine model of chronic hepatitis B virus, Clin Exp Immunol, 166:134–142. DIO: 10.1111/j.1365-2249.2011.04445.x
17 Xia Sh, Sha H, Yang L, Ji Y, Ostrand-Rosenberg S, Qi L (2011) Gr-1 CD11b Myeloid-derived suppressor cells suppress inflammation and promote insulin sensitivity in obesity, J Biol Chem, 286 (26): 23591–23599. DIO: 10.1074/jbc.M111.237123
18 Goni O, Alcaide P, Fresno M (2002) Immunosuppression during acute Trypanosoma cruzi infection: involvement of Ly6G (Gr1(+)) CD11b(+) immature myeloid suppressor cells, Int Immunol, 14(10):1125-1131. DIO: 10.1093/intimm/dxf076
19 Mencacci A, Montagnoli C, Bacci A, Cenci E, Pitzurra L, Spreca A, Kopf M, Sharpe AH, Romani L (2002) CD80+Gr-1+ myeloid cells inhibit development of antifungal Th1 immunity in mice with candidiasis, J Immunol, 169: 3180-3189. DIO: 10.4049/jimmunol.169.6.3180
20 European Convention for the Protection of Vertebrate Animals used for Experimental and Other Scientific Purposes (1986) European Treaty Series – No: 123, Strasbourg.
21 Subiza JL,Vinuela JE, Rodriguez R, Gil J, Figueredo MA, De la Concha EG (1989) Development of splenic natural suppressor (NS) cells in Ehrlich tumor-bearing mice, Int J Cancer, 44:307-314. DIO: 10.1002/ijc.2910440220
1 Prasad S., Sung B., Aggarwal B.B. Age-associated chronic diseases require age-old medicine: Role of chronic inflammation // Prev Med.- 2012.- N54.– P.29–37. DOI:10.1016/j.ypmed.2011.11.011
2 Yu B.P., Chung H.Y. The inflammatory process in aging // Rev Clin Gerontol.- 2006.- N16.– P.179–187. DOI: 10.1017/S0959259807002110
3 Lavrovsky Y, Chatterjee B, Clark R.A., Roy A.K. Role of redox-regulated transcription factors in inflammation, aging and age-related diseases // Exp Gerontol.- 2000.- N35.– P.521–532. DOI:10.1016/S0531-5565(00)00118-2
4 Rahman I. Oxidative stress, chromatin remodeling and gene transcription in inflammation and chronic lung diseases // J Biochem Mol Biol.- 2003.- N36.– P.95–109. DIO: 10.5483/BMBRep.2003.36.1.095
5 Aggarwal B.B. Nuclear factor-kB: the enemy within // Cancer Cell.- 2004 - N6.– P.203–208. DIO:10.1016/j.ccr.2004.09.003
6 Roy S., Bagchi D., Raychandhuri S.P. Chronic inflammation Molecular pathophysiology, nutritional and therapeutic interventions. – USA: CRC Press, Talor@Francis Group L.L.C.- 2013.- 472 p.
7 Zeyda M., Farmer D., Todoric J., Aszmann O., Speiser M., Györi G., Zlabinger G.J., Stulnig T.M. Human adipose tissue macrophages are of an anti-inflammatory phenotype but capable of excessive pro-inflammatory mediator production // Int J Obes.- 2007.- N31.– P.1420–1428. DIO: 10.1038/sj.ijo.0803632
8 Wolf A.M., Wolf D., Rumpold H., Enrich B., Tilg H. Adiponectin induces the anti-inflammatory cytokines IL-10 and IL-1RA in human leukocytes // Biochem Biophys Res Com.- 2004.- N323.– P.630–635. DIO: 10.1016/j.bbrc.2004.08.145
9 Lan H.Y. Diverse roles of TGF-β/Smads in renal fibrosis and Inflammation// Int J Biol Sci.- 2011.- N7.– P.1056-1067.DIO: 10.7150/ijbs.7.1056
10 Han G., Li F., Singh T.P., Wolf P., Wang X.J. The Pro-inflammatory Role of TGFβ1: A Paradox? // Int J Biol S.- 2012.- N8(2).– P.228-235. DIO: 10.7150/ijbs.8.228
11 Gabrilovich D.I., Nagaraj S. Myeloid-derived suppressor cells as regulators of the immune system // Nature Rev Immunol.- 2009.- N9.– P.162-174. DIO: 10.1038/nri2506
12 Greten T.F., Manns M.P., Korangy F. Myeloid derived suppressor cells in human diseases // Intern Immunopharm.- 2011.- N11.- P.802–807. DIO: 10.1016/j.intimp.2011.01.003
13 Ostrand-Rosenberg S., Sinha P. Myeloid-derived suppressor cells: Linking inflammation and cancer // J Immunol.- 2009.- N182.- P.4499–4506. DOI: 10.4049/jimmunol.0802740
14 Peranzoni E., Zilio S., Marigo I., Dolcetti L., Zanovello P., Mandruzzato S., Bronte V. Myeloid-derived suppressor cell heterogeneity and subset definition // Curr Opin Immunol.- 2010.- N22.- P.238–244. DIO: 10.1016/j.coi.2010.01.021
15 Obregón-Henao A., Henao-Tamayo M., Orme I.M., Ordway D.J. Gr1intCD11b+ Myeloid-derived suppressor cells in Mycobacterium tuberculosis Infection // PLOS ONE.- 2013.- N8.- Issue 11 e80669. DIO: 10.1371/journal.pone.0080669
16 Chen S., Akbar S.M., Abe M., Hiasa Y., Onji M. Immunosuppressive functions of hepatic myeloid-derived suppressor cells of normal mice and in a murine model of chronic hepatitis B virus // Clin Exp Immunol.- 2011.- N166.- P.134–142. DIO: 10.1111/j.1365-2249.2011.04445.x
17 Xia S.h., Sha H., Yang L., Ji Y., Ostrand-Rosenberg S., Qi L. Gr-1 CD11b Myeloid-derived suppressor cells suppress inflammation and promote insulin sensitivity in obesity // J Biol Chem.- 2011.- N286(26).- P.23591–23599. DIO: 10.1074/jbc.M111.237123
18 Goni O., Alcaide P., Fresno M. Immunosuppression during acute Trypanosoma cruzi infection: involvement of Ly6G (Gr1(+)) CD11b(+) immature myeloid suppressor cells // Int Immunol.- 2002.- N14(10).- P.1125-1131. DIO: 10.1093/intimm/dxf076
19 Mencacci A., Montagnoli C., Bacci A., Cenci E., Pitzurra L., Spreca A., Kopf M., Sharpe A.H., Romani L. CD80+Gr-1+ myeloid cells inhibit development of antifungal Th1 immunity in mice with candidiasis // J Immunol.- 2002.- N169.- P.3180-3189. DIO: 10.4049/jimmunol.169.6.3180
20 European Convention for the Protection of Vertebrate Animals used for Experimental and Other Scientific Purposes // CETS 123. Strasbourg.- 1986.- 11 p.
21 Subiza J.L.,Vinuela J.E., Rodriguez R., Gil J., Figueredo M.A., De la Concha E.G. Development of splenic natural suppressor (NS) cells in Ehrlich tumor-bearing mice // Int J Cancer.- 1989.- N44.- P307-314. DIO: 10.1002/ijc.2910440220
References
1 Prasad S, Sung B, Aggarwal BB (2012) Age-associated chronic diseases require age-old medicine: Role of chronic inflammation, Prev Med, 54: S29–S37. DOI:10.1016/j.ypmed.2011.11.011
2 Yu BP, Chung HY (2006) The inflammatory process in aging, Rev Clin Gerontol, 16:. 179–187. DOI: 10.1017/S0959259807002110
3 Lavrovsky Y, Chatterjee B, Clark RA, Roy AK (2000) Role of redox-regulated transcription factors in inflammation, aging and age-related diseases, Exp Gerontol, 35: 521–532. DOI:10.1016/S0531-5565(00)00118-2
4 Rahman I (2003) Oxidative stress, chromatin remodeling and gene transcription in inflammation and chronic lung diseases,J Biochem Mol Biol, 36: 95–109. DIO: 10.5483/BMBRep.2003.36.1.095
5 Aggarwal BB (2004) Nuclear factor-kB: the enemy within, Cancer Cell, 6: 203–208. DIO:10.1016/j.ccr.2004.09.003
6 Roy S, Bagchi D, Raychandhuri SP (2013) Chronic inflammation Molecular pathophysiology, nutritional and therapeutic interventions, CRC Press, Talor@Francis Group L.L.C., USA. ISBN:978-1-4398-7211-6
7 Zeyda M, Farmer D, Todoric J, Aszmann O, Speiser M, Györi G, Zlabinger GJ, Stulnig TM (2007) Human adipose tissue macrophages are of an anti-inflammatory phenotype but capable of excessive pro-inflammatory mediator production,Int J Obes, 31: 1420–1428. DIO: 10.1038/sj.ijo.0803632
8 Wolf AM, Wolf D, Rumpold H, Enrich B, Tilg H (2004) Adiponectin induces the anti-inflammatory cytokines IL-10 and IL-1RA in human leukocytes, Biochem Biophys Res Com, 323: 630–635. DIO: 10.1016/j.bbrc.2004.08.145
9 Lan HY (2011) Diverse roles of TGF-β/Smads in renal fibrosis and Inflammation, Int J Biol Sci, 7: 1056-1067.DIO: 10.7150/ijbs.7.1056
10 Han G, Li F, Singh TP, Wolf P, Wang XJ (2012) The Pro-inflammatory Role of TGFβ1: A Paradox?, Int J Biol S, 8(2):228-235. DIO: 10.7150/ijbs.8.228
11 Gabrilovich DI, Nagaraj S (2009) Myeloid-derived suppressor cells as regulators of the immune system, Nature Rev Immunol, 9:162-174. DIO: 10.1038/nri2506
12 Greten TF, Manns MP, Korangy F (2011) Myeloid derived suppressor cells in human diseases, Intern Immunopharm, 11: 802–807. DIO: 10.1016/j.intimp.2011.01.003
13 Ostrand-Rosenberg S, Sinha P (2009) Myeloid-derived suppressor cells: Linking inflammation and cancer, J Immunol, 182: 4499–4506. DOI: 10.4049/jimmunol.0802740
14 Peranzoni E, Zilio S, Marigo I, Dolcetti L, Zanovello P, Mandruzzato S, Bronte V (2010) Myeloid-derived suppressor cell heterogeneity and subset definition, Curr Opin Immunol, 22: 238–244. DIO: 10.1016/j.coi.2010.01.021
15 Obregón-Henao A, Henao-Tamayo M, Orme IM, Ordway DJ (2013) Gr1intCD11b+ Myeloid-derived suppressor cells in Mycobacterium tuberculosis Infection, PLOS ONE, 8: Issue 11 e80669. DIO: 10.1371/journal.pone.0080669
16 Chen S, Akbar SM, Abe M, Hiasa Y, Onji M (2011) Immunosuppressive functions of hepatic myeloid-derived suppressor cells of normal mice and in a murine model of chronic hepatitis B virus, Clin Exp Immunol, 166:134–142. DIO: 10.1111/j.1365-2249.2011.04445.x
17 Xia Sh, Sha H, Yang L, Ji Y, Ostrand-Rosenberg S, Qi L (2011) Gr-1 CD11b Myeloid-derived suppressor cells suppress inflammation and promote insulin sensitivity in obesity, J Biol Chem, 286 (26): 23591–23599. DIO: 10.1074/jbc.M111.237123
18 Goni O, Alcaide P, Fresno M (2002) Immunosuppression during acute Trypanosoma cruzi infection: involvement of Ly6G (Gr1(+)) CD11b(+) immature myeloid suppressor cells, Int Immunol, 14(10):1125-1131. DIO: 10.1093/intimm/dxf076
19 Mencacci A, Montagnoli C, Bacci A, Cenci E, Pitzurra L, Spreca A, Kopf M, Sharpe AH, Romani L (2002) CD80+Gr-1+ myeloid cells inhibit development of antifungal Th1 immunity in mice with candidiasis, J Immunol, 169: 3180-3189. DIO: 10.4049/jimmunol.169.6.3180
20 European Convention for the Protection of Vertebrate Animals used for Experimental and Other Scientific Purposes (1986) European Treaty Series – No: 123, Strasbourg.
21 Subiza JL,Vinuela JE, Rodriguez R, Gil J, Figueredo MA, De la Concha EG (1989) Development of splenic natural suppressor (NS) cells in Ehrlich tumor-bearing mice, Int J Cancer, 44:307-314. DIO: 10.1002/ijc.2910440220
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Abdolla, N., Perfilyeva, Y. V., Ostapchuk, Y. O., Oskolchenko, I. A., Krasnoshtanov, V. K., Severova, E. A., Pominova, N. M., Denisov, Y. D., & Belyaev, N. N. (2016). Тәжірибелік созылмалы қабыну кезіндегі миелоидық супрессорлық жасушалар. ҚазҰУ Хабаршысы. Биология сериясы, 67(2), 178–186. вилучено із https://bb.kaznu.kz/index.php/biology/article/view/1193
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