ПОИСК ДНК-МАРКЕРОВ УСТОЙЧИВОСТИ ЯЧМЕНЯ К СТЕБЛЕВОЙ РЖАВЧИНЕ / АРПАНЫҢ САБАҚТЫҢ ТАТЫНА ТӨЗІМДІЛІГІНІҢ ДНҚ-МАРКЕРЛЕРІН ІЗДЕУ
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Кілттік сөздер:
ячмень, стеблевая ржавчина, ДНК-маркеры, устойчивость, урожайность, арпа, сабақтың таты, ДНҚ-маркерлер, төзімділік, өнімділік.Аннотация
Қазақстанның жаздық арпасының 96 сорттары мен линияларынан құралған коллекцияның сабақтың татына төзімділігі Биологиялық қауіпсіздік проблемалары институтында (Жамбыл ауданы) далалық инфекциялық фонда текерілді. Арпа үлгілерінің төзімділігі бойынша инфекциялық фонда алынған нәтижелер Қазақстанның жеті әртүрлі аумақтарында (2009-2014) зерттелген коллекцияның өнімділігін бағалау арқылы талданды. Жалпы саны 81 ДНҚ-маркерлері қолданылған микросателлитті талдау негізінде сабақтың татына төзімділікпен статистикалық тығыз байланысқан 9 маркер анықталды. Жаздық арпаның сабақтың татына төзімді линиялары жекелеген аймақтардағы арпа селекциясы бойынша жобалар үшін таңдалып алынды. Алынған нәтижелер Қазақстандағы арпаның төзімді және өнімді сорттарын шығаруға бағытталған селекциялық зерттеулердің тиімділігін арттыру үшін қолданылуы мүмкін. Коллекция из 96 сортов и линий ярового ячменя из Казахстана изучена на устойчивость к стеблевой ржавчине на инфекционном фоне в полевых условиях Института проблем биологической безопасности (Жамбылская область). Полученные данные по устойчивости ячменя на инфекционном фоне проанализированы в связи с оценкой урожайности изученной коллекции в естественных условиях семи различных регионов Казахстана (2009-2014 гг.). В результате микросателитного анализа коллекции с использованием 81 ДНК-маркера, идентифицировано 9 SSR-маркеров, статистически достоверно связанных с устойчивостью к стеблевой ржавчине. Отобраны устойчивые линии ячменя с целью использования в различных региональных проектах по селекции ячменя. Полученные данные могут быть использованы для повышения эффективности селекционных исследований по созданию устойчивых и продуктивных сортов ячменя.Библиографиялық сілтемелер
ЛИТЕРАТУРА
1 Комитет по статистике Министерства национальной экономики РК. http://www.kps.kz/astana/company/komitet_po_statistike_ministerstva_natsionalnoy_ekonomiki_rk
2 Turuspekov Y., Sariev B., Chudinov V., Sereda G., Tokhetova L., Ortaev A., Tsygankov V., Doszhanov M., Volis S., Abugalieva S. Genotype x environment interaction patterns for grain yield of spring barley in different regions of Kazakhstan // Генетика. – 2013, том 49, № 2. – С.196-205.
3 Койшибаев М. Болезни зерновых культур.- Алматы: «Бастау», 2002. - 367 с.
4 Рсалиев Ш.С. Устойчивость пшеницы и ячменя к ржавчинным болезням в Казахстане // «Современное состояние и перспективы развития генетики и селекции зерновых культур в Казахстане». – Алматы. – 2012. – С.96-100.
5 Moscou M.J., Lauter N., Steffenson B., Wise R.P. Quantitative and qualitative stem rust resistance factors in barley are associated with transcriptional suppression of defense regulons // PLoS Genet. – 2011. – Vol. 7. - N7. – P. e1002208.
7. Steffenson B.J., Jin Y. Resistance to race ttks of Puccinia graminis f. sp. tritici in barley // Phytopathology. – 2006. – Vol. 96. – P. 5110.
8. Ballini E., Lauter N., Wise R. Prospects for advancing defense to cereal rusts through genetical genomics // Front Plant Sci. – 2013. – Vol. 1. ¬¬– №4. – P.117.
9. Zhou H., Steffenson B.J., Muehlbauer G., Wanyera R., Njau P., Ndeda S. Association mapping of stem rust race TTKSK resistance in US barley breeding germplasm // Theoretical and Applied Genetics. –2014. –Vol.127. –N.6. –P.1293-304.
10. Dracatos P., Singh D., Fetch T. Jr, Park R. Resistance to Puccinia graminis f. sp. avenae in Barley Is Associated with the Rpg5 Locus // Phytopathology. – 2015. – Vol.105. –N.4. –P.490-494.
11. Brueggeman R., Rostoks N., Kudrna D., Kilian A., Han F., Chen J., Druka A., Steffenson B., Kleinhofs A. The barley stem rustresistance gene Rpg1 is a novel disease-resistance gene with homology to receptor kinases // PNAS. –2002. –Vol.99. –P.9328-9333.
12. Druka A., KudrnaD., Han F., KilianA., Steffenson A., Frisch D., Tomkins J., Wing R., Kleinhofs A. Physical mapping of the barley stem rust resistance gene rpg4 // Molecular and General Genetics. – 2000. – Vol.264. – P. 283-290.
13. Turuspekov Y., Sariev B., Abugalieva A., Chudinov V., Sereda G., Tokhetova L., Ortaev A., Tsygankov V., Yespanov A., Blake T., Sato K., Abugalieva S. Comparative genetic and phenotypic variation of USA and Kazakhstan barley accessions // Book of Abstracts of EUCARPIA Cereals Section – ITMI Joint Conference. U.Lohwasser & A.Börner (eds.), Wernigerode 2014a, P.188.
14. Turuspekov Ye., Rsaliev S., Rsaliev A., Tokhetova L., Chudinov V., Sereda G., Ortaev A., Sariev B., Abugalieva S. Resistance to stem rust in spring barley from Kazakhstan // 1st International Workshop on Barley Leaf Diseases, Salsomaggiore Terme, 03-06 June 2014b. P.75.
15. Туруспеков Е.К. Состояние и перспективы развития молекулярной генетики ячменя в Казахстане // Вестник КазНУ. Серия биологическая. - 2010. - №3(45). – С. 90-96.
16. Методы заражения растений и учета результатов в селекции // Гешеле Э.Э. Основы фитопатологической оценки в селекции растений. – Москва: Колос, 1978. – С.129-159.
17. Stakman E.C., Levine M.N. The determination of biologic forms of Puccinia graminis on Triticum spp. // Minn. Agr. Exp. St. Technol. Bull. – 1922. – N.8 – P.38-41.
18. Peterson R.F., Campbell A.B., Hannah A.E. A diagrammatic scale for estimating rust intensity on leaves and stems of cereals // Canadian Journal of Research. – 1948. – Vol.26. – P.496-500.
19. Delaporta S.L., Wood J., Hicks J.B. A plant DNA minipreparation. Version II // Plant Mol. Biol. Rep. – 1983. – V.4. – P. 19-21.
20. Абугалиева С.И., Туруспеков Е.К. ДНК-маркеры в генетике и селекции зерновых культур: научно-методические рекомендации. – Алматы: «I see real», ISBN 9965-9272-4-3. 2009. – 84 с.
REFERENCES
1. Committee on statistics of the Ministry of National economics of Republic of Kazakhstan. http://www.kps.kz/astana/company/komitet_po_statistike_ministerstva_natsionalnoy_ekonomiki_rk
2. Turuspekov Y., Sariev B., Chudinov V., Sereda G., Tokhetova L., Ortaev A., Tsygankov V., Doszhanov M., Volis S., Abugalieva S. Genotype x environment interaction patterns for grain yield of spring barley in different regions of Kazakhstan. Genetika, 2013, vol. 49, no.2. pp. 196-205.
3. Koyshibaev M. Diseases of cereal crops, Almaty, Bastau, 2002. 367 p.
4. Rsaliev Sh.S. Resistance of wheat and barley to rust diseases in Kazakhstan. Proc. of the republican seminar “Current state and prospects of development of genetics and selection of grain crops in Kazakhstan. Almaty, 2012, pp. 96-100.
5. Steffenson B. J., Jin Y., Rossnagel B. G., Rasmussen J. B., Kao K. Genetics of multiple disease resistance in a doubled-haploid population of barley. Plant Breeding, 1995, vol.114, pp. 50-54.
6. Moscou M.J., Lauter N., Steffenson B., Wise R.P. Quantitative and qualitative stem rust resistance factors in barley are associated with transcriptional suppression of defense regulons. PLoS Genet, 2011, vol. 7, no.7, pp. e1002208.
7. Steffenson B.J., Jin Y. Resistance to race ttks of Puccinia graminis f. sp. tritici in barley. Phytopathology, 2006, vol. 96, pp. 5110.
8. Ballini E., Lauter N., Wise R. Prospects for advancing defense to cereal rusts through genetical genomics. Frontiers in Plant Science, 2013, vol. 1, no.4, pp. 117.
9. Zhou H., Steffenson B.J., Muehlbauer G., Wanyera R., Njau P., Ndeda S. Association mapping of stem rust race TTKSK resistance in US barley breeding germplasm. Theoretical and Applied Genetics, 2014, vol.127, no.6, pp. 1293-1304.
10. Dracatos P., Singh D., Fetch T. Jr, Park R. Resistance to Puccinia graminis f. sp. avenae in Barley Is Associated with the Rpg5 Locus. Phytopathology, 2015, vol. 105, no.4, pp.490-494.
11. Brueggeman R., Rostoks N., Kudrna D., Kilian A., Han F., Chen J., Druka A., Steffenson B., Kleinhofs A. The barley stem rustresistance gene Rpg1 is a novel disease-resistance gene with homology to receptor kinases. PNAS, 2002, vol. 99, pp. 9328-9333.
12. Druka A., Kudrna D., Han F., KilianA., Steffenson A., Frisch D., Tomkins J., Wing R., Kleinhofs A. Physical mapping of the barley stem rust resistance gene rpg4. Molecular and General Genetics, 2000, vol.264, pp. 283-290.
13. Turuspekov Y., Sariev B., Abugalieva A., Chudinov V., Sereda G., Tokhetova L., Ortaev A., Tsygankov V., Yespanov A., Blake T., Sato K., Abugalieva S. Comparative genetic and phenotypic variation of USA and Kazakhstan barley accessions // Book of Abstracts of EUCARPIA Cereals Section – ITMI Joint Conference. U.Lohwasser & A.Börner (eds.), Wernigerode 2014a, P.188.
14. Turuspekov Ye., Rsaliev S., Rsaliev A., Tokhetova L., Chudinov V., Sereda G., Ortaev A., Sariev B., Abugalieva S. Resistance to stem rust in spring barley from Kazakhstan. 1st International Workshop on Barley Leaf Diseases, Salsomaggiore Terme, 03-06 June 2014b. P.75.
15. Turuspekov Y.K. State and prospects of development of molecular genetics of barley in Kazakhstan. Bulletin of KazNU. Biology. - 2010. - №3(45). – С. 90-96.
16. Plant infection methods and value of its results in plant breeding. Geshele E.E. Bases of a phytopathologic assessment in plant breeding. Moscow, Kolos, 1978, pp. 129-159.
17. Stakman E.C., Levine M.N. The determination of biologic forms of Puccinia graminis on Triticum spp. Minnesota agricultural experiment station bulletin, 1922, vol.8, pp. 38-41.
18. Peterson R.F., Campbell A.B., Hannah A.E. A diagrammatic scale for estimating rust intensity on leaves and stems of cereals. Canadian Journal of Research, 1948, vol.26, pp. 496-500.
19. Delaporta S.L., Wood J., Hicks J.B. A plant DNA minipreparation. Version II. Plant Molecular Biology Reporter, 1983, vol.4, pp. 19-21.
20. Abugalieva S.I., Turuspekov Ye.K. DNA markers in genetics and breeding of cereal crops: methodical recommendations. Almaty, I see real, 2009, 84 p.
1 Комитет по статистике Министерства национальной экономики РК. http://www.kps.kz/astana/company/komitet_po_statistike_ministerstva_natsionalnoy_ekonomiki_rk
2 Turuspekov Y., Sariev B., Chudinov V., Sereda G., Tokhetova L., Ortaev A., Tsygankov V., Doszhanov M., Volis S., Abugalieva S. Genotype x environment interaction patterns for grain yield of spring barley in different regions of Kazakhstan // Генетика. – 2013, том 49, № 2. – С.196-205.
3 Койшибаев М. Болезни зерновых культур.- Алматы: «Бастау», 2002. - 367 с.
4 Рсалиев Ш.С. Устойчивость пшеницы и ячменя к ржавчинным болезням в Казахстане // «Современное состояние и перспективы развития генетики и селекции зерновых культур в Казахстане». – Алматы. – 2012. – С.96-100.
5 Moscou M.J., Lauter N., Steffenson B., Wise R.P. Quantitative and qualitative stem rust resistance factors in barley are associated with transcriptional suppression of defense regulons // PLoS Genet. – 2011. – Vol. 7. - N7. – P. e1002208.
7. Steffenson B.J., Jin Y. Resistance to race ttks of Puccinia graminis f. sp. tritici in barley // Phytopathology. – 2006. – Vol. 96. – P. 5110.
8. Ballini E., Lauter N., Wise R. Prospects for advancing defense to cereal rusts through genetical genomics // Front Plant Sci. – 2013. – Vol. 1. ¬¬– №4. – P.117.
9. Zhou H., Steffenson B.J., Muehlbauer G., Wanyera R., Njau P., Ndeda S. Association mapping of stem rust race TTKSK resistance in US barley breeding germplasm // Theoretical and Applied Genetics. –2014. –Vol.127. –N.6. –P.1293-304.
10. Dracatos P., Singh D., Fetch T. Jr, Park R. Resistance to Puccinia graminis f. sp. avenae in Barley Is Associated with the Rpg5 Locus // Phytopathology. – 2015. – Vol.105. –N.4. –P.490-494.
11. Brueggeman R., Rostoks N., Kudrna D., Kilian A., Han F., Chen J., Druka A., Steffenson B., Kleinhofs A. The barley stem rustresistance gene Rpg1 is a novel disease-resistance gene with homology to receptor kinases // PNAS. –2002. –Vol.99. –P.9328-9333.
12. Druka A., KudrnaD., Han F., KilianA., Steffenson A., Frisch D., Tomkins J., Wing R., Kleinhofs A. Physical mapping of the barley stem rust resistance gene rpg4 // Molecular and General Genetics. – 2000. – Vol.264. – P. 283-290.
13. Turuspekov Y., Sariev B., Abugalieva A., Chudinov V., Sereda G., Tokhetova L., Ortaev A., Tsygankov V., Yespanov A., Blake T., Sato K., Abugalieva S. Comparative genetic and phenotypic variation of USA and Kazakhstan barley accessions // Book of Abstracts of EUCARPIA Cereals Section – ITMI Joint Conference. U.Lohwasser & A.Börner (eds.), Wernigerode 2014a, P.188.
14. Turuspekov Ye., Rsaliev S., Rsaliev A., Tokhetova L., Chudinov V., Sereda G., Ortaev A., Sariev B., Abugalieva S. Resistance to stem rust in spring barley from Kazakhstan // 1st International Workshop on Barley Leaf Diseases, Salsomaggiore Terme, 03-06 June 2014b. P.75.
15. Туруспеков Е.К. Состояние и перспективы развития молекулярной генетики ячменя в Казахстане // Вестник КазНУ. Серия биологическая. - 2010. - №3(45). – С. 90-96.
16. Методы заражения растений и учета результатов в селекции // Гешеле Э.Э. Основы фитопатологической оценки в селекции растений. – Москва: Колос, 1978. – С.129-159.
17. Stakman E.C., Levine M.N. The determination of biologic forms of Puccinia graminis on Triticum spp. // Minn. Agr. Exp. St. Technol. Bull. – 1922. – N.8 – P.38-41.
18. Peterson R.F., Campbell A.B., Hannah A.E. A diagrammatic scale for estimating rust intensity on leaves and stems of cereals // Canadian Journal of Research. – 1948. – Vol.26. – P.496-500.
19. Delaporta S.L., Wood J., Hicks J.B. A plant DNA minipreparation. Version II // Plant Mol. Biol. Rep. – 1983. – V.4. – P. 19-21.
20. Абугалиева С.И., Туруспеков Е.К. ДНК-маркеры в генетике и селекции зерновых культур: научно-методические рекомендации. – Алматы: «I see real», ISBN 9965-9272-4-3. 2009. – 84 с.
REFERENCES
1. Committee on statistics of the Ministry of National economics of Republic of Kazakhstan. http://www.kps.kz/astana/company/komitet_po_statistike_ministerstva_natsionalnoy_ekonomiki_rk
2. Turuspekov Y., Sariev B., Chudinov V., Sereda G., Tokhetova L., Ortaev A., Tsygankov V., Doszhanov M., Volis S., Abugalieva S. Genotype x environment interaction patterns for grain yield of spring barley in different regions of Kazakhstan. Genetika, 2013, vol. 49, no.2. pp. 196-205.
3. Koyshibaev M. Diseases of cereal crops, Almaty, Bastau, 2002. 367 p.
4. Rsaliev Sh.S. Resistance of wheat and barley to rust diseases in Kazakhstan. Proc. of the republican seminar “Current state and prospects of development of genetics and selection of grain crops in Kazakhstan. Almaty, 2012, pp. 96-100.
5. Steffenson B. J., Jin Y., Rossnagel B. G., Rasmussen J. B., Kao K. Genetics of multiple disease resistance in a doubled-haploid population of barley. Plant Breeding, 1995, vol.114, pp. 50-54.
6. Moscou M.J., Lauter N., Steffenson B., Wise R.P. Quantitative and qualitative stem rust resistance factors in barley are associated with transcriptional suppression of defense regulons. PLoS Genet, 2011, vol. 7, no.7, pp. e1002208.
7. Steffenson B.J., Jin Y. Resistance to race ttks of Puccinia graminis f. sp. tritici in barley. Phytopathology, 2006, vol. 96, pp. 5110.
8. Ballini E., Lauter N., Wise R. Prospects for advancing defense to cereal rusts through genetical genomics. Frontiers in Plant Science, 2013, vol. 1, no.4, pp. 117.
9. Zhou H., Steffenson B.J., Muehlbauer G., Wanyera R., Njau P., Ndeda S. Association mapping of stem rust race TTKSK resistance in US barley breeding germplasm. Theoretical and Applied Genetics, 2014, vol.127, no.6, pp. 1293-1304.
10. Dracatos P., Singh D., Fetch T. Jr, Park R. Resistance to Puccinia graminis f. sp. avenae in Barley Is Associated with the Rpg5 Locus. Phytopathology, 2015, vol. 105, no.4, pp.490-494.
11. Brueggeman R., Rostoks N., Kudrna D., Kilian A., Han F., Chen J., Druka A., Steffenson B., Kleinhofs A. The barley stem rustresistance gene Rpg1 is a novel disease-resistance gene with homology to receptor kinases. PNAS, 2002, vol. 99, pp. 9328-9333.
12. Druka A., Kudrna D., Han F., KilianA., Steffenson A., Frisch D., Tomkins J., Wing R., Kleinhofs A. Physical mapping of the barley stem rust resistance gene rpg4. Molecular and General Genetics, 2000, vol.264, pp. 283-290.
13. Turuspekov Y., Sariev B., Abugalieva A., Chudinov V., Sereda G., Tokhetova L., Ortaev A., Tsygankov V., Yespanov A., Blake T., Sato K., Abugalieva S. Comparative genetic and phenotypic variation of USA and Kazakhstan barley accessions // Book of Abstracts of EUCARPIA Cereals Section – ITMI Joint Conference. U.Lohwasser & A.Börner (eds.), Wernigerode 2014a, P.188.
14. Turuspekov Ye., Rsaliev S., Rsaliev A., Tokhetova L., Chudinov V., Sereda G., Ortaev A., Sariev B., Abugalieva S. Resistance to stem rust in spring barley from Kazakhstan. 1st International Workshop on Barley Leaf Diseases, Salsomaggiore Terme, 03-06 June 2014b. P.75.
15. Turuspekov Y.K. State and prospects of development of molecular genetics of barley in Kazakhstan. Bulletin of KazNU. Biology. - 2010. - №3(45). – С. 90-96.
16. Plant infection methods and value of its results in plant breeding. Geshele E.E. Bases of a phytopathologic assessment in plant breeding. Moscow, Kolos, 1978, pp. 129-159.
17. Stakman E.C., Levine M.N. The determination of biologic forms of Puccinia graminis on Triticum spp. Minnesota agricultural experiment station bulletin, 1922, vol.8, pp. 38-41.
18. Peterson R.F., Campbell A.B., Hannah A.E. A diagrammatic scale for estimating rust intensity on leaves and stems of cereals. Canadian Journal of Research, 1948, vol.26, pp. 496-500.
19. Delaporta S.L., Wood J., Hicks J.B. A plant DNA minipreparation. Version II. Plant Molecular Biology Reporter, 1983, vol.4, pp. 19-21.
20. Abugalieva S.I., Turuspekov Ye.K. DNA markers in genetics and breeding of cereal crops: methodical recommendations. Almaty, I see real, 2009, 84 p.
Жүктелулер
Как цитировать
Turuspekov, Y. K., Rsaliev, S. S., Rsaliev, A. S., Yermekbaev, K. A., & Abugalieva, S. I. (2016). ПОИСК ДНК-МАРКЕРОВ УСТОЙЧИВОСТИ ЯЧМЕНЯ К СТЕБЛЕВОЙ РЖАВЧИНЕ / АРПАНЫҢ САБАҚТЫҢ ТАТЫНА ТӨЗІМДІЛІГІНІҢ ДНҚ-МАРКЕРЛЕРІН ІЗДЕУ. ҚазҰУ Хабаршысы. Биология сериясы, 65(3), 162–169. вилучено із https://bb.kaznu.kz/index.php/biology/article/view/1112
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МОЛЕКУЛЯРНАЯ БИОЛОГИЯ И ГЕНЕТИКА
