THE SEARCH OF DNA MARKERS ASSOCIATED WITH BARLEY RESISTANCE TO STEM RUST

Authors

  • Y. K. Turuspekov Институт биологии и биотехнологии растений КН МОН РК, г. Алматы, Казахстан
  • Sh. S. Rsaliev Институт проблем биологической безопасности КН МОН РК, п.г.т. Гвардейский, Джамбульская область, Казахстан
  • A. S. Rsaliev Институт проблем биологической безопасности КН МОН РК, п.г.т. Гвардейский, Джамбульская область, Казахстан
  • K. A. Yermekbaev Институт биологии и биотехнологии растений КН МОН РК, г. Алматы, Казахстан
  • S. I. Abugalieva Институт биологии и биотехнологии растений КН МОН РК, г. Алматы, Казахстан
        127 78

Keywords:

barley, stem rust, DNA markers, resistance, yield.

Abstract

The collection of 96 cultivars and promising lines of spring barley from Kazakhstan was studied for resistance to stem rust by using artificially infected field conditions of the Research Institute of problems for Biological Safety (Zhambul region). Obtained results of barley resistance at infected conditions were studied in relationship to grain yield recorded at 7 different locations of Kazakhstan during the period from 2009 to 2014. In addition, the results of 81 microsatellite markers screened for all 96 barley accessions were allowed to identify 9 DNA markers that statistically wise reliably associated with resistance to stem rust. A number of stem rust resistant lines for stem rust were identified for different regional barley breeding programs. The results of this work can be successfully used in studies associated with improved and efficient breeding for disease resistance and higher grain yield of barley in Kazakhstan.

References

ЛИТЕРАТУРА

1 Комитет по статистике Министерства национальной экономики РК. http://www.kps.kz/astana/company/komitet_po_statistike_ministerstva_natsionalnoy_ekonomiki_rk
2 Turuspekov Y., Sariev B., Chudinov V., Sereda G., Tokhetova L., Ortaev A., Tsygankov V., Doszhanov M., Volis S., Abugalieva S. Genotype x environment interaction patterns for grain yield of spring barley in different regions of Kazakhstan // Генетика. – 2013, том 49, № 2. – С.196-205.
3 Койшибаев М. Болезни зерновых культур.- Алматы: «Бастау», 2002. - 367 с.
4 Рсалиев Ш.С. Устойчивость пшеницы и ячменя к ржавчинным болезням в Казахстане // «Современное состояние и перспективы развития генетики и селекции зерновых культур в Казахстане». – Алматы. – 2012. – С.96-100.
5 Moscou M.J., Lauter N., Steffenson B., Wise R.P. Quantitative and qualitative stem rust resistance factors in barley are associated with transcriptional suppression of defense regulons // PLoS Genet. – 2011. – Vol. 7. - N7. – P. e1002208.
7. Steffenson B.J., Jin Y. Resistance to race ttks of Puccinia graminis f. sp. tritici in barley // Phytopathology. – 2006. – Vol. 96. – P. 5110.
8. Ballini E., Lauter N., Wise R. Prospects for advancing defense to cereal rusts through genetical genomics // Front Plant Sci. – 2013. – Vol. 1. ¬¬– №4. – P.117.
9. Zhou H., Steffenson B.J., Muehlbauer G., Wanyera R., Njau P., Ndeda S. Association mapping of stem rust race TTKSK resistance in US barley breeding germplasm // Theoretical and Applied Genetics. –2014. –Vol.127. –N.6. –P.1293-304.
10. Dracatos P., Singh D., Fetch T. Jr, Park R. Resistance to Puccinia graminis f. sp. avenae in Barley Is Associated with the Rpg5 Locus // Phytopathology. – 2015. – Vol.105. –N.4. –P.490-494.
11. Brueggeman R., Rostoks N., Kudrna D., Kilian A., Han F., Chen J., Druka A., Steffenson B., Kleinhofs A. The barley stem rustresistance gene Rpg1 is a novel disease-resistance gene with homology to receptor kinases // PNAS. –2002. –Vol.99. –P.9328-9333.
12. Druka A., KudrnaD., Han F., KilianA., Steffenson A., Frisch D., Tomkins J., Wing R., Kleinhofs A. Physical mapping of the barley stem rust resistance gene rpg4 // Molecular and General Genetics. – 2000. – Vol.264. – P. 283-290.
13. Turuspekov Y., Sariev B., Abugalieva A., Chudinov V., Sereda G., Tokhetova L., Ortaev A., Tsygankov V., Yespanov A., Blake T., Sato K., Abugalieva S. Comparative genetic and phenotypic variation of USA and Kazakhstan barley accessions // Book of Abstracts of EUCARPIA Cereals Section – ITMI Joint Conference. U.Lohwasser & A.Börner (eds.), Wernigerode 2014a, P.188.
14. Turuspekov Ye., Rsaliev S., Rsaliev A., Tokhetova L., Chudinov V., Sereda G., Ortaev A., Sariev B., Abugalieva S. Resistance to stem rust in spring barley from Kazakhstan // 1st International Workshop on Barley Leaf Diseases, Salsomaggiore Terme, 03-06 June 2014b. P.75.
15. Туруспеков Е.К. Состояние и перспективы развития молекулярной генетики ячменя в Казахстане // Вестник КазНУ. Серия биологическая. - 2010. - №3(45). – С. 90-96.
16. Методы заражения растений и учета результатов в селекции // Гешеле Э.Э. Основы фитопатологической оценки в селекции растений. – Москва: Колос, 1978. – С.129-159.
17. Stakman E.C., Levine M.N. The determination of biologic forms of Puccinia graminis on Triticum spp. // Minn. Agr. Exp. St. Technol. Bull. – 1922. – N.8 – P.38-41.
18. Peterson R.F., Campbell A.B., Hannah A.E. A diagrammatic scale for estimating rust intensity on leaves and stems of cereals // Canadian Journal of Research. – 1948. – Vol.26. – P.496-500.
19. Delaporta S.L., Wood J., Hicks J.B. A plant DNA minipreparation. Version II // Plant Mol. Biol. Rep. – 1983. – V.4. – P. 19-21.
20. Абугалиева С.И., Туруспеков Е.К. ДНК-маркеры в генетике и селекции зерновых культур: научно-методические рекомендации. – Алматы: «I see real», ISBN 9965-9272-4-3. 2009. – 84 с.

REFERENCES

1. Committee on statistics of the Ministry of National economics of Republic of Kazakhstan. http://www.kps.kz/astana/company/komitet_po_statistike_ministerstva_natsionalnoy_ekonomiki_rk
2. Turuspekov Y., Sariev B., Chudinov V., Sereda G., Tokhetova L., Ortaev A., Tsygankov V., Doszhanov M., Volis S., Abugalieva S. Genotype x environment interaction patterns for grain yield of spring barley in different regions of Kazakhstan. Genetika, 2013, vol. 49, no.2. pp. 196-205.
3. Koyshibaev M. Diseases of cereal crops, Almaty, Bastau, 2002. 367 p.
4. Rsaliev Sh.S. Resistance of wheat and barley to rust diseases in Kazakhstan. Proc. of the republican seminar “Current state and prospects of development of genetics and selection of grain crops in Kazakhstan. Almaty, 2012, pp. 96-100.
5. Steffenson B. J., Jin Y., Rossnagel B. G., Rasmussen J. B., Kao K. Genetics of multiple disease resistance in a doubled-haploid population of barley. Plant Breeding, 1995, vol.114, pp. 50-54.
6. Moscou M.J., Lauter N., Steffenson B., Wise R.P. Quantitative and qualitative stem rust resistance factors in barley are associated with transcriptional suppression of defense regulons. PLoS Genet, 2011, vol. 7, no.7, pp. e1002208.
7. Steffenson B.J., Jin Y. Resistance to race ttks of Puccinia graminis f. sp. tritici in barley. Phytopathology, 2006, vol. 96, pp. 5110.
8. Ballini E., Lauter N., Wise R. Prospects for advancing defense to cereal rusts through genetical genomics. Frontiers in Plant Science, 2013, vol. 1, no.4, pp. 117.
9. Zhou H., Steffenson B.J., Muehlbauer G., Wanyera R., Njau P., Ndeda S. Association mapping of stem rust race TTKSK resistance in US barley breeding germplasm. Theoretical and Applied Genetics, 2014, vol.127, no.6, pp. 1293-1304.
10. Dracatos P., Singh D., Fetch T. Jr, Park R. Resistance to Puccinia graminis f. sp. avenae in Barley Is Associated with the Rpg5 Locus. Phytopathology, 2015, vol. 105, no.4, pp.490-494.
11. Brueggeman R., Rostoks N., Kudrna D., Kilian A., Han F., Chen J., Druka A., Steffenson B., Kleinhofs A. The barley stem rustresistance gene Rpg1 is a novel disease-resistance gene with homology to receptor kinases. PNAS, 2002, vol. 99, pp. 9328-9333.
12. Druka A., Kudrna D., Han F., KilianA., Steffenson A., Frisch D., Tomkins J., Wing R., Kleinhofs A. Physical mapping of the barley stem rust resistance gene rpg4. Molecular and General Genetics, 2000, vol.264, pp. 283-290.
13. Turuspekov Y., Sariev B., Abugalieva A., Chudinov V., Sereda G., Tokhetova L., Ortaev A., Tsygankov V., Yespanov A., Blake T., Sato K., Abugalieva S. Comparative genetic and phenotypic variation of USA and Kazakhstan barley accessions // Book of Abstracts of EUCARPIA Cereals Section – ITMI Joint Conference. U.Lohwasser & A.Börner (eds.), Wernigerode 2014a, P.188.
14. Turuspekov Ye., Rsaliev S., Rsaliev A., Tokhetova L., Chudinov V., Sereda G., Ortaev A., Sariev B., Abugalieva S. Resistance to stem rust in spring barley from Kazakhstan. 1st International Workshop on Barley Leaf Diseases, Salsomaggiore Terme, 03-06 June 2014b. P.75.
15. Turuspekov Y.K. State and prospects of development of molecular genetics of barley in Kazakhstan. Bulletin of KazNU. Biology. - 2010. - №3(45). – С. 90-96.
16. Plant infection methods and value of its results in plant breeding. Geshele E.E. Bases of a phytopathologic assessment in plant breeding. Moscow, Kolos, 1978, pp. 129-159.
17. Stakman E.C., Levine M.N. The determination of biologic forms of Puccinia graminis on Triticum spp. Minnesota agricultural experiment station bulletin, 1922, vol.8, pp. 38-41.
18. Peterson R.F., Campbell A.B., Hannah A.E. A diagrammatic scale for estimating rust intensity on leaves and stems of cereals. Canadian Journal of Research, 1948, vol.26, pp. 496-500.
19. Delaporta S.L., Wood J., Hicks J.B. A plant DNA minipreparation. Version II. Plant Molecular Biology Reporter, 1983, vol.4, pp. 19-21.
20. Abugalieva S.I., Turuspekov Ye.K. DNA markers in genetics and breeding of cereal crops: methodical recommendations. Almaty, I see real, 2009, 84 p.

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How to Cite

Turuspekov, Y. K., Rsaliev, S. S., Rsaliev, A. S., Yermekbaev, K. A., & Abugalieva, S. I. (2016). THE SEARCH OF DNA MARKERS ASSOCIATED WITH BARLEY RESISTANCE TO STEM RUST. Experimental Biology, 65(3), 162–169. Retrieved from https://bb.kaznu.kz/index.php/biology/article/view/1112

Issue

Vol. 65 No. 3 (2015): KazNU Bulletin. Biology series

Section

МOLECULAR BIOLOGY AND GENETICS

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