ХАРАКТЕРИСТИКА И АМПЛИФИКАЦИЯ ГЕНА АЛЬФА-ФЕТОПРОТЕИНА ЧЕЛОВЕКА
63 47
Keywords:
pre-mRNA, cDNA of HAFP by RT – PCR,Abstract
The latent signals of splice site according to rules of processing of pre-mRNA in nucleus of plant cells was revealed. Optimal vector for cloning cDNA of HAFP and computer modeling of recombinant constructions design containing his-tag on the C - end of protein has been carried out. Amplification cDNA of HAFP by RT – PCR has been carried out.References
1 Abelev G. Alpha-fetoprotein: 25 years of study // Tumor Biol. 1989. V. 10. P. 63-74.
2 Torres J.M., Darracq N., Uriel J. Membrane proteins from lymphoblastoid cells showing crossaffinity for alpha-fetoprotein and albumin. Isolation and characterization // Biochim.Biophys. Acta. 1992.
v.1159. P. 60-66.
3 Nunez E.A. Biological role of alpha-fetoprotein in the endocrinological field: data and hypotheses. // Tumor Biol. 1994. v. 15. P. 63-72.
4 Mizejewski GJ Alpha-fetoprotein structure and function: relevance to isoforms, epitopes, and conformational variants // Experimental biology and medicine (Maywood, N.J.). 2001. v 226 (5): 377–408.
5 Аймбетов Р.С., Полимбетова Н.С., Бисенбаев А.К., Искаков Б.К. Амплификация гена альфа- фетопротеина человека для экспрессии в трансгенных растениях// Вестник КазНУ. Серия биолог. -
2008. - №1(36). - С. 89-90.
6 Brown J. W. S. and Simpson C. G. Splice site selection in plant pre-mRNA splicing . // Annual Review of Plant Physiology and Plant Molecular Biology. 1998.v.49: 77-95 .
7 Punchapat S., Norene B. and Hugh S. M. A plant signal peptide–hepatitis B surface antigen fusion protein with enhanced stability and immunogenicity expressed in plant cells // Proc Natl Acad Sci U S A.,
2003. v.100 (5). 2209 –2214.
8 Akbergenov R., Zhanybekova S., Polimbetova N., Madin K., Hohn T., Iskakov B. Complementary interaction between the central domain of 18S rRNA and the 5’ untranslated region of mRNA enhances
translation efficiency in plants // In: “Cell-Free Protein Expression”, Ed. J.R. Swartz, Springer-Verlag. - 2003. - P. 199-208.
2 Torres J.M., Darracq N., Uriel J. Membrane proteins from lymphoblastoid cells showing crossaffinity for alpha-fetoprotein and albumin. Isolation and characterization // Biochim.Biophys. Acta. 1992.
v.1159. P. 60-66.
3 Nunez E.A. Biological role of alpha-fetoprotein in the endocrinological field: data and hypotheses. // Tumor Biol. 1994. v. 15. P. 63-72.
4 Mizejewski GJ Alpha-fetoprotein structure and function: relevance to isoforms, epitopes, and conformational variants // Experimental biology and medicine (Maywood, N.J.). 2001. v 226 (5): 377–408.
5 Аймбетов Р.С., Полимбетова Н.С., Бисенбаев А.К., Искаков Б.К. Амплификация гена альфа- фетопротеина человека для экспрессии в трансгенных растениях// Вестник КазНУ. Серия биолог. -
2008. - №1(36). - С. 89-90.
6 Brown J. W. S. and Simpson C. G. Splice site selection in plant pre-mRNA splicing . // Annual Review of Plant Physiology and Plant Molecular Biology. 1998.v.49: 77-95 .
7 Punchapat S., Norene B. and Hugh S. M. A plant signal peptide–hepatitis B surface antigen fusion protein with enhanced stability and immunogenicity expressed in plant cells // Proc Natl Acad Sci U S A.,
2003. v.100 (5). 2209 –2214.
8 Akbergenov R., Zhanybekova S., Polimbetova N., Madin K., Hohn T., Iskakov B. Complementary interaction between the central domain of 18S rRNA and the 5’ untranslated region of mRNA enhances
translation efficiency in plants // In: “Cell-Free Protein Expression”, Ed. J.R. Swartz, Springer-Verlag. - 2003. - P. 199-208.
Downloads
How to Cite
Аймбетов, Р. С., Полимбетова, Л. П., Станбекова, Г. С., Бисенбаев, А. К., & Искаков, Б. К. (2009). ХАРАКТЕРИСТИКА И АМПЛИФИКАЦИЯ ГЕНА АЛЬФА-ФЕТОПРОТЕИНА ЧЕЛОВЕКА. Experimental Biology, 42(3), 45–50. Retrieved from https://bb.kaznu.kz/index.php/biology/article/view/710
Issue
Section
GENETICS and MOLECULAR BIOLOGY
