Изучение генов устойчивости к бурой ржавчине с помощью молекулярных маркеров у мягкой пшеницы (Triticum aestivum L.) Молекулалық маркерлер арқылы жұмсақ бидайда (Triticum aestivum L.) қоңыр татқа төзімді гендерін зерттеу
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Keywords:
бурая ржавчина, SCAR, STS, маркеры, устойчивость, ген, ДНК, пшеница. қоңыр тат ауруы, STS маркерлер, төзімділік, ДНҚ, бидайAbstract
С помощью молекулярных SCAR и STS-маркеров Iag95, Lr28, csLV34, Sr39, VENTRIUP-LN2, WMS382 проведен скрининг сортообразцов и линий местной селекции на устойчивость к бурой ржавчине по генам Lr26,Lr28, Lr34, Lr35, Lr37 и Lr50. У 9 сортообразцов и диких видов - T. timopheevii и T. kiharae по специфическим продуктам амплификации ДНК было обнаружено наличие гена Lr26. У 19 сортообразцов, в том числе и линий имели ген Lr34, ген Lr35 был выявлен только у дикого вида Ae. kotschyi, а ген Lr37 был обнаружен в интрогрессивной линии л-344 и у диких видов Ae. ventricosa, T. timopheevii, T. kiharae. Ген Lr50 был обнаружен у 21 из изучаемых 50 образцов пшеницы Алғаш рет SCAR жəне STS молекулалық маркерлерді Iag95, Lr28, csLV34, Sr39, VENTRIUP-LN2, WMS382 қолдану арқылы жергілікті селекция сортүлгілеріне жəне линияларға қоңыр тат ауруына төзімді Lr26, Lr28, Lr34, Lr35, Lr37 жəне Lr50 гендеріне скрининг жүргізілді. ДНҚ амплификациясы нəтижесінде түзілген өнім бойынша 9 сортүлгіде жəне жабайы түрлерде - T. timopheevii, T. kiharae Lr26 гені анықталды. 19 сортүлгі мен линияларда Lr34 гені, Lr35 гені жабайы түр – Ae. kotschyi, Lr37 гені интрогрессивті линия л-344 пен жабайы түрлер – Ae. ventricosa, T. timopheevii, T. kiharae идентификацияланды. Зерттеліп отырған 50 сортүлгілерінің 21 үлгісінде Lr50 гені табылды.References
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7. Lagudah E.S., McFadden H., Singh R.P., Huerta-Espino J., Bariana H.S., Spielmeyer W. Molecular genetic
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8. Gold J, Harder D, Townley-Smith F. Development of molecular marker for rust resistance genes Sr39 and Lr35 in wheat breeding lines. http://www.ejbiotechnology.cl/content/vol2/issue1/full/1/index.html. – 2002. P. 624-631.
9. Helguera M, Khan IA, Kolmer J, Lijavetzky D, Zhong-qi L, Dubcovsky J. PCR assays for the Lr37-Yr17-
Sr38 cluster of rust resistance genes and their use to develop isogenic hard red spring wheat lines // Crop Science. –2003. – Vol. 43. – P. 1839-1847.
10. Brown-Guedira GL, Singh S, Fritz AK. Performance and mapping of leaf rust resistance transferred to wheat
from Triticum timopheevii ssp. Armeniacum // Phytopathology. – 2003. – Vol. 93. – No. 7. – P. 784-789
11. Moreno-Sevilla B., Baenziger P.S. The 1BL/1RS translocation: Agronomic performance of F3-derived lines from a
winter wheat cross // Crop Sci. – 1995. – V. 35. – P.1051-1055.
12. Blaszczyk L, Chelkowski J, Korzun V, Kraic J, Ordon F, Ovesná J, Purnhauser L, Tar M, Vida G. Verification of
STS markers for leaf rust resistance genes of wheat by seven European laboratories // Cell. Mol. Biol. Lett. –
symbols for wheat. Proceedings of the 11th International Wheat Genetics Symposium. Brisbane, Australia. – 2008. P.15–32.
2. Kuchel H., Ye G., Fox R., Jefferies S. Genetic and economic analysis of a targeted marker-assisted wheat breeding
strategy // Molecular Breeding. - 2005. – V.16. P. 67–78.
3. Singh R.P., William H.M., Huerta-Espino J., Rosewarne G. Wheat rust in Asia: meeting the challenges with old and
new technologies. "New directions for a diverse planet". Proceedings of the 4th International Crop Science Congress,
26 Sep – 1 Oct. Brisbane, Australia. Published on CDROM. Web site www.cropscience.org.au. – 2004. P. 27-54.
4. Edwards K., Jonstone C., Thompson C.A. Simple and rspid method for the preparation of plant genomic DNA for
PCR analysis // Nucl. Acids Res. – 1991. – Vol. 19. – No. 6. – P. 1349.
5. Mago R., Spielmeyer W., Lawrence G.J., Lagudah E.S., Ellis J.G., Pryor A. Identification and mapping of molecular
markers linked to rust resistance genes located on chromosome 1RS of rye using wheat-rye translocation lines // Theor.
Appl. Genet. – 2002. – Vol. 104. – P.1317-1324.
6. Naik S., Gill K.S., Prakasa Rao V.S., Gupta V.S., Tamhankar S.A., Putjar S., Gill B.S., Ranjekar P.K.. Identification
of a STS marker linked to the Aegilops speltoides-derived leaf rust resistance gene Lr28 in wheat // Theor. Appl. Genet.– 1998. – Vol. 97. – P. 535-540.
7. Lagudah E.S., McFadden H., Singh R.P., Huerta-Espino J., Bariana H.S., Spielmeyer W. Molecular genetic
characterization of the Lr34/Yr18 slow rusting resistance gene region in wheat // Theor. Appl. Genet. – 2006. – Vol.
114. – P. 21-30.
8. Gold J, Harder D, Townley-Smith F. Development of molecular marker for rust resistance genes Sr39 and Lr35 in wheat breeding lines. http://www.ejbiotechnology.cl/content/vol2/issue1/full/1/index.html. – 2002. P. 624-631.
9. Helguera M, Khan IA, Kolmer J, Lijavetzky D, Zhong-qi L, Dubcovsky J. PCR assays for the Lr37-Yr17-
Sr38 cluster of rust resistance genes and their use to develop isogenic hard red spring wheat lines // Crop Science. –2003. – Vol. 43. – P. 1839-1847.
10. Brown-Guedira GL, Singh S, Fritz AK. Performance and mapping of leaf rust resistance transferred to wheat
from Triticum timopheevii ssp. Armeniacum // Phytopathology. – 2003. – Vol. 93. – No. 7. – P. 784-789
11. Moreno-Sevilla B., Baenziger P.S. The 1BL/1RS translocation: Agronomic performance of F3-derived lines from a
winter wheat cross // Crop Sci. – 1995. – V. 35. – P.1051-1055.
12. Blaszczyk L, Chelkowski J, Korzun V, Kraic J, Ordon F, Ovesná J, Purnhauser L, Tar M, Vida G. Verification of
STS markers for leaf rust resistance genes of wheat by seven European laboratories // Cell. Mol. Biol. Lett. –
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Tokubayeva, A. A., & Shulembaeva, K. K. (2015). Изучение генов устойчивости к бурой ржавчине с помощью молекулярных маркеров у мягкой пшеницы (Triticum aestivum L.) Молекулалық маркерлер арқылы жұмсақ бидайда (Triticum aestivum L.) қоңыр татқа төзімді гендерін зерттеу. Experimental Biology, 59(3/1), 188–192. Retrieved from https://bb.kaznu.kz/index.php/biology/article/view/686
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