Идентификация генов устойчивости к бурой ржавчине Lr26, Lr28, Lr34, Lr37 с помощью STS и SCAR маркеров у мягкой пшеницы (Triticum aestivum L.).SCAR және STS маркерлері арқылы жұмсақ бидайда қоңыр татқа төзімді LR26, LR28, LR34, LR37 гендерін идентификация
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Ключевые слова:
бурая ржавчина, SCAR, STS маркеры, устойчивость, ген, ДНК, пшеница. қоңыр тат ауруы, STS маркерлер, төзімділік, ДНҚ, бидай.Аннотация
С помощью молекулярных SCAR и STS-маркеров Iag95, Lr28, csLV34, VENTRIUP-LN2 проведен скрининг сортообразцов и линий местной селекции на устойчивость к бурой ржавчине по генам Lr26, Lr28, Lr34 и Lr37.У 9 сортообразцов и диких видов - Tr. timopheevii и Tr. kiharae по специфическим продуктам амплификации ДНК было обнаружено наличие гена Lr26. 19 сортообразцов, в том числе и линий имели ген Lr34, а ген Lr37 был обнаружен в интрогрессивной линии л-344 и у диких видов Ae. ventricosa, Tr. timopheevii, Tr. kiharae. Маркеры Lr28-01, Lr28-02, тесно сцепленные с геном Lr28, амплифицировали специфический фрагмент 378 п.н. у всех образцов. Выявленные ДНК маркеры Lr28-01, Lr28-02 не позволяют надежно идентифицировать образцы пшеницы, имеющие гены возрастной устойчивости Lr28, так как наличие этого гена обнаружено у всех нами изученных сортообразцов, и наряду с этим у высоковосприимчивого сорта Тhatcher. Lr26 гені жұмсақ бидайдың 9 сорттары мен бидай үлгілерінде және Tr. timopheevii мен Tr. kiharae жабайы түрлерінде идентификацияланған. Алғаш рет Iag95, csLV34, VENTRIUP-LN2 молекулалық маркерлерді қолдану арқылы жергілікті селекция сорттарының көп бөлігінде жоғары эффективті Lr26, Lr34 және Lr37 гендері идентификацияланды.Библиографические ссылки
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5 Cherukuri D.P., Gupta S.K., Charpe A., Koul S., Prabhu K.V., Singh R.B., Haq Q.M.R., Chauhan S.V.S. Identification of a
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9 Mago R., Spielmeyer W., Lawrence G.J., Lagudah E.S., Ellis J.G., Pryor A. Identification and mapping of molecular markers
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104: – P.1317-1324.
10 Naik S., Gill K.S., Prakasa Rao V.S., Gupta V.S., Tamhankar S.A., Putjar S., Gill B.S., Ranjekar P.K.. Identification of a STS
marker linked to the Aegilops speltoides-derived leaf rust resistance gene Lr28 in wheat // Theor. Appl. Genet. – 1998. – V. 97. – P. 535-
540.
11 Lagudah E.S., McFadden H., Singh R.P., Huerta-Espino J., Bariana H.S., Spielmeyer W. Molecular genetic characterization
of the Lr34/Yr18 slow rusting resistance gene region in wheat // Theor. Appl. Genet. – 2006. V. 114. – P. 21-30.
12 Singh R.P., Huerta-Espino J. Effect of leaf rust resistance gene Lr34 on components of slow rusting at seven growth stages in
wheat // In: Euphytica. – 2003. – V. 129. – P.371-376.
13 Rajaram S., Mann C.H.E., Ortiz-Ferrara G., Mujeeb-Kazi A. Adaptation, stability and high yield potential of certain 1B/1R
CIMMYT wheats. In S Sakamoto, ed., Proc. 6th Int. Wheat Genetics Symp. – Kyoto, 1983. – P. 613-621.
14 Gul’tyaeva E.I., Kanyuka I.A., Alpat’eva N.V., Baranova O.A., Dmitriev A.P., Pavlyushin VA. Molecular approaches in
identifying leaf rust resistance genes in russian wheat varieties // Russian Agricultural Sciences. – 2009. – V. 35(5). – 316–319
15 Moreno-Sevilla B., Baenziger P.S. The 1BL/1RS translocation: Agronomic performance of F3-derived lines from a winter
wheat cross // Crop Sci. – 1995. – V. 35.- P.1051-1055.
16 McIntosh R.A., Miller T.E., Chapman V. Cytogenetical studies in wheat. XII. Lr28 for esistance to Puccinia recondita and
Sr34 for resistance to P. graminis tritici. Z. Pflanzenzuchtg. – 1982. – V. 89. – P. 295-306.
17 Dyck P.L., Samborski D.J., Anderson R.G. Inheritance of adult-plant leaf rust resistance derived from the common wheat
varieties Exchange and Frontana // In: Canadian Journal of Genetics and Cytology. – 1966. –V. 8. – P. 665-671.
18 Singh RP, Rajaram S. Genetics of adult plant resistance of leaf rust in Frontana and CIMMYT wheat // Genome. – 1992. – V.
5. – P. 24-31.
aestivum L.) cultivars using molecular markers // Russian Journal of Genetics. – 2006. – V.42(5). – P. 546–554.
2 McIntosh R.A., Yamazaki Y., Dubcovsky J., Rogers W.J., Morris C.F., Somers D.J. et al. Catalogue of gene symbols for wheat.Proceedings of the 11th International Wheat Genetics Symposium. Brisbane, Australia. – 2008.
3 McIntosh R.A., Yamazaki Y., Rogers W.J., Morris C.F., Devos K.M. Catalogue of gene symbols for wheat (http://www.shigen.
nig.ac.jp/wheat/komugi/genes/ download.jsp). – 2010.
4 Riar A.K., Kaur S., Dhaliwal H.S., Singh K., Chhuneja P. Introgression of a leaf rust resistance gene from Aegilops caudate
to bread wheat // Journal of Genetics. – 2012. – V.91(2). – P. 155-161.
5 Cherukuri D.P., Gupta S.K., Charpe A., Koul S., Prabhu K.V., Singh R.B., Haq Q.M.R., Chauhan S.V.S. Identification of a
molecular marker linked to an Agropyron elongatum-derived gene Lr19 for leaf rust resistance in wheat // Plant Breed. – 2003. – V.122.
– P. 204–208.
6 Landjeva S., Korzun V., Burner A. Molecular markers: actual and potential contributions to wheat genome haracterization and
breeding // Euphytica. – 2007. – V.156. – P. 271–296.
Kuchel H., Ye G., Fox R., Jefferies S. Genetic and economic analysis of a targeted marker-assisted wheat breeding strategy //
Molecular Breeding. – 2005. – V.16. P. 67–78.
7 Singh R.P., William H.M., Huerta-Espino J., Rosewarne G. Wheat rust in Asia: meeting the challenges with old and new
technologies. “New directions for a diverse planet”. Proceedings of the 4th International Crop Science Congress, 26 Sep – 1 Oct.
Brisbane, Australia. Published on CDROM. Web site www.cropscience.org.au. – 2004.
8 Edwards K., Jonstone C., Thompson C.A. Simple and rspid method for the preparation of plant genomic DNA for PCR
analysis // Nucl. Acids Res. – 1991. – V. 19(6). – P. 1349.
9 Mago R., Spielmeyer W., Lawrence G.J., Lagudah E.S., Ellis J.G., Pryor A. Identification and mapping of molecular markers
linked to rust resistance genes located on chromosome 1RS of rye using wheat-rye translocation lines // Theor. Appl. Genet. – 2002. – V.
104: – P.1317-1324.
10 Naik S., Gill K.S., Prakasa Rao V.S., Gupta V.S., Tamhankar S.A., Putjar S., Gill B.S., Ranjekar P.K.. Identification of a STS
marker linked to the Aegilops speltoides-derived leaf rust resistance gene Lr28 in wheat // Theor. Appl. Genet. – 1998. – V. 97. – P. 535-
540.
11 Lagudah E.S., McFadden H., Singh R.P., Huerta-Espino J., Bariana H.S., Spielmeyer W. Molecular genetic characterization
of the Lr34/Yr18 slow rusting resistance gene region in wheat // Theor. Appl. Genet. – 2006. V. 114. – P. 21-30.
12 Singh R.P., Huerta-Espino J. Effect of leaf rust resistance gene Lr34 on components of slow rusting at seven growth stages in
wheat // In: Euphytica. – 2003. – V. 129. – P.371-376.
13 Rajaram S., Mann C.H.E., Ortiz-Ferrara G., Mujeeb-Kazi A. Adaptation, stability and high yield potential of certain 1B/1R
CIMMYT wheats. In S Sakamoto, ed., Proc. 6th Int. Wheat Genetics Symp. – Kyoto, 1983. – P. 613-621.
14 Gul’tyaeva E.I., Kanyuka I.A., Alpat’eva N.V., Baranova O.A., Dmitriev A.P., Pavlyushin VA. Molecular approaches in
identifying leaf rust resistance genes in russian wheat varieties // Russian Agricultural Sciences. – 2009. – V. 35(5). – 316–319
15 Moreno-Sevilla B., Baenziger P.S. The 1BL/1RS translocation: Agronomic performance of F3-derived lines from a winter
wheat cross // Crop Sci. – 1995. – V. 35.- P.1051-1055.
16 McIntosh R.A., Miller T.E., Chapman V. Cytogenetical studies in wheat. XII. Lr28 for esistance to Puccinia recondita and
Sr34 for resistance to P. graminis tritici. Z. Pflanzenzuchtg. – 1982. – V. 89. – P. 295-306.
17 Dyck P.L., Samborski D.J., Anderson R.G. Inheritance of adult-plant leaf rust resistance derived from the common wheat
varieties Exchange and Frontana // In: Canadian Journal of Genetics and Cytology. – 1966. –V. 8. – P. 665-671.
18 Singh RP, Rajaram S. Genetics of adult plant resistance of leaf rust in Frontana and CIMMYT wheat // Genome. – 1992. – V.
5. – P. 24-31.
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Как цитировать
Tokubayeva A. А., & Shulembaeva, K. K. (2015). Идентификация генов устойчивости к бурой ржавчине Lr26, Lr28, Lr34, Lr37 с помощью STS и SCAR маркеров у мягкой пшеницы (Triticum aestivum L.).SCAR және STS маркерлері арқылы жұмсақ бидайда қоңыр татқа төзімді LR26, LR28, LR34, LR37 гендерін идентификация. Вестник КазНУ. Серия биологическая, 57(1), 158–164. извлечено от https://bb.kaznu.kz/index.php/biology/article/view/227
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