Study of high molecular weight hyaluronan influence on functional activity of human T regulatory cells
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Keywords:
T-regulatory cells, immunomagnetic cell separation, flow cytofluorimetry, hyaluronan, interleukin-10, MTT-test.Abstract
The work is devoted to study the extracellular matrix component, high molecular weight hyaluronan, on the suppressor activity of CD4+CD25+FoxP3+ Treg cells, which were obtained from healthy human peripheral blood. Hyaluronan has been established to increase suppressor activity of Treg cells in part of donors; in other individuals it caused a decrease in this parameter. Hyaluronan did not influence on the production of IL-10 by Treg cells. In its turn suppressor activity has not been associated with IL-10 production by Treg cells. Some mechanisms of hyaluronan infulence on the suppression by Treg cells are discussed. Experimental approaches worked out in this study, can be useful for investigation of the impaired Treg-dependent immunosupression in pathologic states as autoimmunity and cancer.References
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interleukin 2 production//J. Exp. Med.-1998.-No.2.-P.287–296.
Immunology.-2006.-Vol.18.-No.7.-P. 991-1000.
2 Baatar D., Olkhanud P., Sumitomo K., Taub D., Gress R., Biragyn A. Human peripheral blood t regulatory cells (Tregs)
functionally primed CCR4+ Tregs and unprimed CCR4+ Tregs, regulate effector T cells using FasL//J.Immunol.-2007.-No.178.-
P.4891-4900.
3 Liu W., Putnam A.L., Xu-yu Z., Szot G.L., Lee M.R., Zhu S., Gottlieb P.A., Kapranov P., Gingeras T.R., Fazekas de St Groth
B., Clayberger C., Soper D.M., Ziegler S.F., Bluestone J.A. CD127 expression inversely correlates with FoxP3 and suppressive function
of human CD4+ Treg cells//JEM. – 2006.-No3. – P.1701-1711.
4 Asano M., Toda M., Sakaguchi N., Sakaguchi S. Autoimmune disease as a consequence of developmental abnormality of a T
cell subpopulation//J. Exp. Med.-1996.-Vol.106.-P.387-396.
5 Cools N., Ponsaerts P., van Tendeloo V.F.I., Berneman Z.N. Regulatory T cells and human disease//Clin. Develop. Immunol.
– 2007. – Vol.2007. – P.1-11.
6 Yang Z.-Z., Novak A.J., Stenson M.J., Witzig T.E., Ansell S.M. Intratumoral CD4+CD25+ regulatory T-cell–mediated suppression
of infiltratingCD4+ T cells in B-cell non-Hodgkin lymphoma//Blood. -2006.- No.9(107). – P.3639-3646.
7 Железникова Г.Ф. Регуляторные Т-лимфоциты в иммунном ответе на инфекцию//Ж. инфектол. – 2011. – №1(3). – C.6-13.
8 Sakaguchi S., Wing K., Onishi Y., Prieto-Martin P., Yamaguchi T. Regulatory T cells: how do they suppress immune responses?//
Int. Immunol. – 2009. – Vol.21. – P.1105-1111.
9 Adair-Kirka T.L., Senior R.M. Fragments of extracellular matrix as mediators of inflammation//Int. J. Biochem. Cell.
Biol.-2008.-N40 (6-7).-P.1101–1110.
10 Bollyky P., Falk B.A., Wu R.P., Buckner J.H., Wight T.N., Nepom G.T. Intact extracellular matrix and the maintenance of
immune tolerance: High molecular weight hyaluronan promotes persistence of induced CD4+CD25+ regulatory T cells//J. Leukocyte
Biol. – 2009. – Vol.86. – P.1-6.
1 Sherman L., Sleeman J., Herrlich P., Ponta H. Hyaluronate receptors: Key players in growth, differentiation, migration and
tumor progression//Curr. Opin. Cell Biol.-1994.-No.6.-P.726-733.
12 Bollyky P.L., Lord J. D., Masewicz S.A., Evanko S.P., Buckner J.H., Wight T.N., Nepom G.T. Cutting edge: High molecular
weight hyaluronan promotes the suppressive effects of CD4+CD25+ regulatory T cells//J. Immunol.-2007.-No.179.-P.744–747.
13 Bollyky P.L., Falk B.A., Long S.A., Preisinger A., Braun K.R., Wu R.P., Evanko S.P., Buckner J.H., Wight T.N., Nepom
G.T. CD44 costimulation promotes FoxP3 regulatory T cell persistence and function via production of IL-2, IL-10, and TGF-β//J.
Immunol.-2009.-No.183.-P.2232–2241.
14 Purе E., Cuff C.A. A crucial role for CD44 in inflammation//Trends Mol. Med.-2001.-No.5(7).-P.213-221.
15 Ponta H., Sherman L., Herrlich P.A. CD44: from adhesion molecules to signalling regulators//Nature Rev.-2003.-Vol.4.-
P.33-45.
16 Carmichael J., DeGraff W.G., Gazdar A.F., Minna J.D. Mitchell J.B. Evaluation of a tetrazolium-based semiautomated colorimetric
assay: assessment of chemosensitivity testing//Cancer Res.-1987.-Vol.47.-P.936-942.
17 Azze R.O., Olivares M.N., Rodríguez J.C.M. Use of poly-l-lysine precoating in an ELISA for the detection of antibodies
against serogroup C Neisseria meningitidis capsular polysaccharide//Biotecnol. Aplicada.-1999.-Vol.16.-No.3.-P.173-175.
18 Pierson W., Liston A. A new role for interleukin-10 in immune regulation// Immunol. Cell Biol.-2010.-Vol.88.-P.769–770.
19 Stern J.B., Smith K.A. Interleukin-2 induction of T-cell G1 progression and c-myb expression//Science.- 1986.-Vol.233.-
P.203–206. http://en.wikipedia.org/wiki/Digital_object_identifier
20 Thornton A.M., Shevach E.M. CD4+CD25+Immunoregulatory T cells suppress polyclonal T cell activation in vitro by inhibiting
interleukin 2 production//J. Exp. Med.-1998.-No.2.-P.287–296.
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Abramova, V. A., Abdolla, N., Perfilieva, Y. V., Ostapchuk, E. O., & Belyaev, N. N. (2015). Study of high molecular weight hyaluronan influence on functional activity of human T regulatory cells. Experimental Biology, 58(2), 129–136. Retrieved from https://bb.kaznu.kz/index.php/biology/article/view/553
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HUMAN and ANIMAL PHYSIOLOGY
