Миелоидные супрессорные клетки при экспериментальном хроническом воспалении
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Ключевые слова:
Хроническое воспаление, MDSC, проточная цитометрия, карциномы Эрлиха, адъювант артритАннотация
Миелоидные супрессорные клетки (MDSC) представляют собой гетерогенную популяцию гемопоэтических предшественников миеломоноцитарного ряда, идентифицированную у мышей по экспрессии клеточных маркеров CD11b и Gr-1, обладающих способностью угнетать противоопухолевую активность NK-клеток и цитотоксических Т-лимфоцитов. Мы предположили, что феномен MDSC не является прерогативой опухолевого процесса и связан с патогенезом хронического воспаления, которое предшествует и, в известной степени, индуцирует онкогенез. Целью работы явилось цитофлуориметрическое исследование различных субпопуляций MDSC у мышей в динамике развития адъювантного артрита, представляющего собой классическую модель экспериментального хронического воспаления. Мы установили, что уже к концу 2-й недели развития хронического воспаления в задней конечности мышей линии CD-1, индуцированного полным адъювантом Фрейнда, происходило более чем двукратное увеличение уровня обеих фракций MDSC (M и G) в селезенке, которое сохранилось к концу 4-й недели. Кроме того, было обнаружено значительное увеличение доли CD11b+Gr-1+ MDSC, экспрессирующих молекулы адгезии/хемотаксиса CD184, CD195 и CD62L, на разных сроках исследования. Таким образом, можно заключить, что хроническое воспаление индуцирует повышение уровня MDSC и их способности мигрировать в очаг воспаления.
Библиографические ссылки
Әдебиеттер
1 Prasad S., Sung B., Aggarwal B.B. Age-associated chronic diseases require age-old medicine: Role of chronic inflammation // Prev Med.- 2012.- N54.– P.29–37. DOI:10.1016/j.ypmed.2011.11.011
2 Yu B.P., Chung H.Y. The inflammatory process in aging // Rev Clin Gerontol.- 2006.- N16.– P.179–187. DOI: 10.1017/S0959259807002110
3 Lavrovsky Y, Chatterjee B, Clark R.A., Roy A.K. Role of redox-regulated transcription factors in inflammation, aging and age-related diseases // Exp Gerontol.- 2000.- N35.– P.521–532. DOI:10.1016/S0531-5565(00)00118-2
4 Rahman I. Oxidative stress, chromatin remodeling and gene transcription in inflammation and chronic lung diseases // J Biochem Mol Biol.- 2003.- N36.– P.95–109. DIO: 10.5483/BMBRep.2003.36.1.095
5 Aggarwal B.B. Nuclear factor-kB: the enemy within // Cancer Cell.- 2004 - N6.– P.203–208. DIO:10.1016/j.ccr.2004.09.003
6 Roy S., Bagchi D., Raychandhuri S.P. Chronic inflammation Molecular pathophysiology, nutritional and therapeutic interventions. – USA: CRC Press, Talor@Francis Group L.L.C.- 2013.- 472 p.
7 Zeyda M., Farmer D., Todoric J., Aszmann O., Speiser M., Györi G., Zlabinger G.J., Stulnig T.M. Human adipose tissue macrophages are of an anti-inflammatory phenotype but capable of excessive pro-inflammatory mediator production // Int J Obes.- 2007.- N31.– P.1420–1428. DIO: 10.1038/sj.ijo.0803632
8 Wolf A.M., Wolf D., Rumpold H., Enrich B., Tilg H. Adiponectin induces the anti-inflammatory cytokines IL-10 and IL-1RA in human leukocytes // Biochem Biophys Res Com.- 2004.- N323.– P.630–635. DIO: 10.1016/j.bbrc.2004.08.145
9 Lan H.Y. Diverse roles of TGF-β/Smads in renal fibrosis and Inflammation// Int J Biol Sci.- 2011.- N7.– P.1056-1067.DIO: 10.7150/ijbs.7.1056
10 Han G., Li F., Singh T.P., Wolf P., Wang X.J. The Pro-inflammatory Role of TGFβ1: A Paradox? // Int J Biol S.- 2012.- N8(2).– P.228-235. DIO: 10.7150/ijbs.8.228
11 Gabrilovich D.I., Nagaraj S. Myeloid-derived suppressor cells as regulators of the immune system // Nature Rev Immunol.- 2009.- N9.– P.162-174. DIO: 10.1038/nri2506
12 Greten T.F., Manns M.P., Korangy F. Myeloid derived suppressor cells in human diseases // Intern Immunopharm.- 2011.- N11.- P.802–807. DIO: 10.1016/j.intimp.2011.01.003
13 Ostrand-Rosenberg S., Sinha P. Myeloid-derived suppressor cells: Linking inflammation and cancer // J Immunol.- 2009.- N182.- P.4499–4506. DOI: 10.4049/jimmunol.0802740
14 Peranzoni E., Zilio S., Marigo I., Dolcetti L., Zanovello P., Mandruzzato S., Bronte V. Myeloid-derived suppressor cell heterogeneity and subset definition // Curr Opin Immunol.- 2010.- N22.- P.238–244. DIO: 10.1016/j.coi.2010.01.021
15 Obregón-Henao A., Henao-Tamayo M., Orme I.M., Ordway D.J. Gr1intCD11b+ Myeloid-derived suppressor cells in Mycobacterium tuberculosis Infection // PLOS ONE.- 2013.- N8.- Issue 11 e80669. DIO: 10.1371/journal.pone.0080669
16 Chen S., Akbar S.M., Abe M., Hiasa Y., Onji M. Immunosuppressive functions of hepatic myeloid-derived suppressor cells of normal mice and in a murine model of chronic hepatitis B virus // Clin Exp Immunol.- 2011.- N166.- P.134–142. DIO: 10.1111/j.1365-2249.2011.04445.x
17 Xia S.h., Sha H., Yang L., Ji Y., Ostrand-Rosenberg S., Qi L. Gr-1 CD11b Myeloid-derived suppressor cells suppress inflammation and promote insulin sensitivity in obesity // J Biol Chem.- 2011.- N286(26).- P.23591–23599. DIO: 10.1074/jbc.M111.237123
18 Goni O., Alcaide P., Fresno M. Immunosuppression during acute Trypanosoma cruzi infection: involvement of Ly6G (Gr1(+)) CD11b(+) immature myeloid suppressor cells // Int Immunol.- 2002.- N14(10).- P.1125-1131. DIO: 10.1093/intimm/dxf076
19 Mencacci A., Montagnoli C., Bacci A., Cenci E., Pitzurra L., Spreca A., Kopf M., Sharpe A.H., Romani L. CD80+Gr-1+ myeloid cells inhibit development of antifungal Th1 immunity in mice with candidiasis // J Immunol.- 2002.- N169.- P.3180-3189. DIO: 10.4049/jimmunol.169.6.3180
20 European Convention for the Protection of Vertebrate Animals used for Experimental and Other Scientific Purposes // CETS 123. Strasbourg.- 1986.- 11 p.
21 Subiza J.L.,Vinuela J.E., Rodriguez R., Gil J., Figueredo M.A., De la Concha E.G. Development of splenic natural suppressor (NS) cells in Ehrlich tumor-bearing mice // Int J Cancer.- 1989.- N44.- P307-314. DIO: 10.1002/ijc.2910440220
References
1 Prasad S, Sung B, Aggarwal BB (2012) Age-associated chronic diseases require age-old medicine: Role of chronic inflammation, Prev Med, 54: S29–S37. DOI:10.1016/j.ypmed.2011.11.011
2 Yu BP, Chung HY (2006) The inflammatory process in aging, Rev Clin Gerontol, 16:. 179–187. DOI: 10.1017/S0959259807002110
3 Lavrovsky Y, Chatterjee B, Clark RA, Roy AK (2000) Role of redox-regulated transcription factors in inflammation, aging and age-related diseases, Exp Gerontol, 35: 521–532. DOI:10.1016/S0531-5565(00)00118-2
4 Rahman I (2003) Oxidative stress, chromatin remodeling and gene transcription in inflammation and chronic lung diseases,J Biochem Mol Biol, 36: 95–109. DIO: 10.5483/BMBRep.2003.36.1.095
5 Aggarwal BB (2004) Nuclear factor-kB: the enemy within, Cancer Cell, 6: 203–208. DIO:10.1016/j.ccr.2004.09.003
6 Roy S, Bagchi D, Raychandhuri SP (2013) Chronic inflammation Molecular pathophysiology, nutritional and therapeutic interventions, CRC Press, Talor@Francis Group L.L.C., USA. ISBN:978-1-4398-7211-6
7 Zeyda M, Farmer D, Todoric J, Aszmann O, Speiser M, Györi G, Zlabinger GJ, Stulnig TM (2007) Human adipose tissue macrophages are of an anti-inflammatory phenotype but capable of excessive pro-inflammatory mediator production,Int J Obes, 31: 1420–1428. DIO: 10.1038/sj.ijo.0803632
8 Wolf AM, Wolf D, Rumpold H, Enrich B, Tilg H (2004) Adiponectin induces the anti-inflammatory cytokines IL-10 and IL-1RA in human leukocytes, Biochem Biophys Res Com, 323: 630–635. DIO: 10.1016/j.bbrc.2004.08.145
9 Lan HY (2011) Diverse roles of TGF-β/Smads in renal fibrosis and Inflammation, Int J Biol Sci, 7: 1056-1067.DIO: 10.7150/ijbs.7.1056
10 Han G, Li F, Singh TP, Wolf P, Wang XJ (2012) The Pro-inflammatory Role of TGFβ1: A Paradox?, Int J Biol S, 8(2):228-235. DIO: 10.7150/ijbs.8.228
11 Gabrilovich DI, Nagaraj S (2009) Myeloid-derived suppressor cells as regulators of the immune system, Nature Rev Immunol, 9:162-174. DIO: 10.1038/nri2506
12 Greten TF, Manns MP, Korangy F (2011) Myeloid derived suppressor cells in human diseases, Intern Immunopharm, 11: 802–807. DIO: 10.1016/j.intimp.2011.01.003
13 Ostrand-Rosenberg S, Sinha P (2009) Myeloid-derived suppressor cells: Linking inflammation and cancer, J Immunol, 182: 4499–4506. DOI: 10.4049/jimmunol.0802740
14 Peranzoni E, Zilio S, Marigo I, Dolcetti L, Zanovello P, Mandruzzato S, Bronte V (2010) Myeloid-derived suppressor cell heterogeneity and subset definition, Curr Opin Immunol, 22: 238–244. DIO: 10.1016/j.coi.2010.01.021
15 Obregón-Henao A, Henao-Tamayo M, Orme IM, Ordway DJ (2013) Gr1intCD11b+ Myeloid-derived suppressor cells in Mycobacterium tuberculosis Infection, PLOS ONE, 8: Issue 11 e80669. DIO: 10.1371/journal.pone.0080669
16 Chen S, Akbar SM, Abe M, Hiasa Y, Onji M (2011) Immunosuppressive functions of hepatic myeloid-derived suppressor cells of normal mice and in a murine model of chronic hepatitis B virus, Clin Exp Immunol, 166:134–142. DIO: 10.1111/j.1365-2249.2011.04445.x
17 Xia Sh, Sha H, Yang L, Ji Y, Ostrand-Rosenberg S, Qi L (2011) Gr-1 CD11b Myeloid-derived suppressor cells suppress inflammation and promote insulin sensitivity in obesity, J Biol Chem, 286 (26): 23591–23599. DIO: 10.1074/jbc.M111.237123
18 Goni O, Alcaide P, Fresno M (2002) Immunosuppression during acute Trypanosoma cruzi infection: involvement of Ly6G (Gr1(+)) CD11b(+) immature myeloid suppressor cells, Int Immunol, 14(10):1125-1131. DIO: 10.1093/intimm/dxf076
19 Mencacci A, Montagnoli C, Bacci A, Cenci E, Pitzurra L, Spreca A, Kopf M, Sharpe AH, Romani L (2002) CD80+Gr-1+ myeloid cells inhibit development of antifungal Th1 immunity in mice with candidiasis, J Immunol, 169: 3180-3189. DIO: 10.4049/jimmunol.169.6.3180
20 European Convention for the Protection of Vertebrate Animals used for Experimental and Other Scientific Purposes (1986) European Treaty Series – No: 123, Strasbourg.
21 Subiza JL,Vinuela JE, Rodriguez R, Gil J, Figueredo MA, De la Concha EG (1989) Development of splenic natural suppressor (NS) cells in Ehrlich tumor-bearing mice, Int J Cancer, 44:307-314. DIO: 10.1002/ijc.2910440220
1 Prasad S., Sung B., Aggarwal B.B. Age-associated chronic diseases require age-old medicine: Role of chronic inflammation // Prev Med.- 2012.- N54.– P.29–37. DOI:10.1016/j.ypmed.2011.11.011
2 Yu B.P., Chung H.Y. The inflammatory process in aging // Rev Clin Gerontol.- 2006.- N16.– P.179–187. DOI: 10.1017/S0959259807002110
3 Lavrovsky Y, Chatterjee B, Clark R.A., Roy A.K. Role of redox-regulated transcription factors in inflammation, aging and age-related diseases // Exp Gerontol.- 2000.- N35.– P.521–532. DOI:10.1016/S0531-5565(00)00118-2
4 Rahman I. Oxidative stress, chromatin remodeling and gene transcription in inflammation and chronic lung diseases // J Biochem Mol Biol.- 2003.- N36.– P.95–109. DIO: 10.5483/BMBRep.2003.36.1.095
5 Aggarwal B.B. Nuclear factor-kB: the enemy within // Cancer Cell.- 2004 - N6.– P.203–208. DIO:10.1016/j.ccr.2004.09.003
6 Roy S., Bagchi D., Raychandhuri S.P. Chronic inflammation Molecular pathophysiology, nutritional and therapeutic interventions. – USA: CRC Press, Talor@Francis Group L.L.C.- 2013.- 472 p.
7 Zeyda M., Farmer D., Todoric J., Aszmann O., Speiser M., Györi G., Zlabinger G.J., Stulnig T.M. Human adipose tissue macrophages are of an anti-inflammatory phenotype but capable of excessive pro-inflammatory mediator production // Int J Obes.- 2007.- N31.– P.1420–1428. DIO: 10.1038/sj.ijo.0803632
8 Wolf A.M., Wolf D., Rumpold H., Enrich B., Tilg H. Adiponectin induces the anti-inflammatory cytokines IL-10 and IL-1RA in human leukocytes // Biochem Biophys Res Com.- 2004.- N323.– P.630–635. DIO: 10.1016/j.bbrc.2004.08.145
9 Lan H.Y. Diverse roles of TGF-β/Smads in renal fibrosis and Inflammation// Int J Biol Sci.- 2011.- N7.– P.1056-1067.DIO: 10.7150/ijbs.7.1056
10 Han G., Li F., Singh T.P., Wolf P., Wang X.J. The Pro-inflammatory Role of TGFβ1: A Paradox? // Int J Biol S.- 2012.- N8(2).– P.228-235. DIO: 10.7150/ijbs.8.228
11 Gabrilovich D.I., Nagaraj S. Myeloid-derived suppressor cells as regulators of the immune system // Nature Rev Immunol.- 2009.- N9.– P.162-174. DIO: 10.1038/nri2506
12 Greten T.F., Manns M.P., Korangy F. Myeloid derived suppressor cells in human diseases // Intern Immunopharm.- 2011.- N11.- P.802–807. DIO: 10.1016/j.intimp.2011.01.003
13 Ostrand-Rosenberg S., Sinha P. Myeloid-derived suppressor cells: Linking inflammation and cancer // J Immunol.- 2009.- N182.- P.4499–4506. DOI: 10.4049/jimmunol.0802740
14 Peranzoni E., Zilio S., Marigo I., Dolcetti L., Zanovello P., Mandruzzato S., Bronte V. Myeloid-derived suppressor cell heterogeneity and subset definition // Curr Opin Immunol.- 2010.- N22.- P.238–244. DIO: 10.1016/j.coi.2010.01.021
15 Obregón-Henao A., Henao-Tamayo M., Orme I.M., Ordway D.J. Gr1intCD11b+ Myeloid-derived suppressor cells in Mycobacterium tuberculosis Infection // PLOS ONE.- 2013.- N8.- Issue 11 e80669. DIO: 10.1371/journal.pone.0080669
16 Chen S., Akbar S.M., Abe M., Hiasa Y., Onji M. Immunosuppressive functions of hepatic myeloid-derived suppressor cells of normal mice and in a murine model of chronic hepatitis B virus // Clin Exp Immunol.- 2011.- N166.- P.134–142. DIO: 10.1111/j.1365-2249.2011.04445.x
17 Xia S.h., Sha H., Yang L., Ji Y., Ostrand-Rosenberg S., Qi L. Gr-1 CD11b Myeloid-derived suppressor cells suppress inflammation and promote insulin sensitivity in obesity // J Biol Chem.- 2011.- N286(26).- P.23591–23599. DIO: 10.1074/jbc.M111.237123
18 Goni O., Alcaide P., Fresno M. Immunosuppression during acute Trypanosoma cruzi infection: involvement of Ly6G (Gr1(+)) CD11b(+) immature myeloid suppressor cells // Int Immunol.- 2002.- N14(10).- P.1125-1131. DIO: 10.1093/intimm/dxf076
19 Mencacci A., Montagnoli C., Bacci A., Cenci E., Pitzurra L., Spreca A., Kopf M., Sharpe A.H., Romani L. CD80+Gr-1+ myeloid cells inhibit development of antifungal Th1 immunity in mice with candidiasis // J Immunol.- 2002.- N169.- P.3180-3189. DIO: 10.4049/jimmunol.169.6.3180
20 European Convention for the Protection of Vertebrate Animals used for Experimental and Other Scientific Purposes // CETS 123. Strasbourg.- 1986.- 11 p.
21 Subiza J.L.,Vinuela J.E., Rodriguez R., Gil J., Figueredo M.A., De la Concha E.G. Development of splenic natural suppressor (NS) cells in Ehrlich tumor-bearing mice // Int J Cancer.- 1989.- N44.- P307-314. DIO: 10.1002/ijc.2910440220
References
1 Prasad S, Sung B, Aggarwal BB (2012) Age-associated chronic diseases require age-old medicine: Role of chronic inflammation, Prev Med, 54: S29–S37. DOI:10.1016/j.ypmed.2011.11.011
2 Yu BP, Chung HY (2006) The inflammatory process in aging, Rev Clin Gerontol, 16:. 179–187. DOI: 10.1017/S0959259807002110
3 Lavrovsky Y, Chatterjee B, Clark RA, Roy AK (2000) Role of redox-regulated transcription factors in inflammation, aging and age-related diseases, Exp Gerontol, 35: 521–532. DOI:10.1016/S0531-5565(00)00118-2
4 Rahman I (2003) Oxidative stress, chromatin remodeling and gene transcription in inflammation and chronic lung diseases,J Biochem Mol Biol, 36: 95–109. DIO: 10.5483/BMBRep.2003.36.1.095
5 Aggarwal BB (2004) Nuclear factor-kB: the enemy within, Cancer Cell, 6: 203–208. DIO:10.1016/j.ccr.2004.09.003
6 Roy S, Bagchi D, Raychandhuri SP (2013) Chronic inflammation Molecular pathophysiology, nutritional and therapeutic interventions, CRC Press, Talor@Francis Group L.L.C., USA. ISBN:978-1-4398-7211-6
7 Zeyda M, Farmer D, Todoric J, Aszmann O, Speiser M, Györi G, Zlabinger GJ, Stulnig TM (2007) Human adipose tissue macrophages are of an anti-inflammatory phenotype but capable of excessive pro-inflammatory mediator production,Int J Obes, 31: 1420–1428. DIO: 10.1038/sj.ijo.0803632
8 Wolf AM, Wolf D, Rumpold H, Enrich B, Tilg H (2004) Adiponectin induces the anti-inflammatory cytokines IL-10 and IL-1RA in human leukocytes, Biochem Biophys Res Com, 323: 630–635. DIO: 10.1016/j.bbrc.2004.08.145
9 Lan HY (2011) Diverse roles of TGF-β/Smads in renal fibrosis and Inflammation, Int J Biol Sci, 7: 1056-1067.DIO: 10.7150/ijbs.7.1056
10 Han G, Li F, Singh TP, Wolf P, Wang XJ (2012) The Pro-inflammatory Role of TGFβ1: A Paradox?, Int J Biol S, 8(2):228-235. DIO: 10.7150/ijbs.8.228
11 Gabrilovich DI, Nagaraj S (2009) Myeloid-derived suppressor cells as regulators of the immune system, Nature Rev Immunol, 9:162-174. DIO: 10.1038/nri2506
12 Greten TF, Manns MP, Korangy F (2011) Myeloid derived suppressor cells in human diseases, Intern Immunopharm, 11: 802–807. DIO: 10.1016/j.intimp.2011.01.003
13 Ostrand-Rosenberg S, Sinha P (2009) Myeloid-derived suppressor cells: Linking inflammation and cancer, J Immunol, 182: 4499–4506. DOI: 10.4049/jimmunol.0802740
14 Peranzoni E, Zilio S, Marigo I, Dolcetti L, Zanovello P, Mandruzzato S, Bronte V (2010) Myeloid-derived suppressor cell heterogeneity and subset definition, Curr Opin Immunol, 22: 238–244. DIO: 10.1016/j.coi.2010.01.021
15 Obregón-Henao A, Henao-Tamayo M, Orme IM, Ordway DJ (2013) Gr1intCD11b+ Myeloid-derived suppressor cells in Mycobacterium tuberculosis Infection, PLOS ONE, 8: Issue 11 e80669. DIO: 10.1371/journal.pone.0080669
16 Chen S, Akbar SM, Abe M, Hiasa Y, Onji M (2011) Immunosuppressive functions of hepatic myeloid-derived suppressor cells of normal mice and in a murine model of chronic hepatitis B virus, Clin Exp Immunol, 166:134–142. DIO: 10.1111/j.1365-2249.2011.04445.x
17 Xia Sh, Sha H, Yang L, Ji Y, Ostrand-Rosenberg S, Qi L (2011) Gr-1 CD11b Myeloid-derived suppressor cells suppress inflammation and promote insulin sensitivity in obesity, J Biol Chem, 286 (26): 23591–23599. DIO: 10.1074/jbc.M111.237123
18 Goni O, Alcaide P, Fresno M (2002) Immunosuppression during acute Trypanosoma cruzi infection: involvement of Ly6G (Gr1(+)) CD11b(+) immature myeloid suppressor cells, Int Immunol, 14(10):1125-1131. DIO: 10.1093/intimm/dxf076
19 Mencacci A, Montagnoli C, Bacci A, Cenci E, Pitzurra L, Spreca A, Kopf M, Sharpe AH, Romani L (2002) CD80+Gr-1+ myeloid cells inhibit development of antifungal Th1 immunity in mice with candidiasis, J Immunol, 169: 3180-3189. DIO: 10.4049/jimmunol.169.6.3180
20 European Convention for the Protection of Vertebrate Animals used for Experimental and Other Scientific Purposes (1986) European Treaty Series – No: 123, Strasbourg.
21 Subiza JL,Vinuela JE, Rodriguez R, Gil J, Figueredo MA, De la Concha EG (1989) Development of splenic natural suppressor (NS) cells in Ehrlich tumor-bearing mice, Int J Cancer, 44:307-314. DIO: 10.1002/ijc.2910440220
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Abdolla, N., Perfilyeva, Y. V., Ostapchuk, Y. O., Oskolchenko, I. A., Krasnoshtanov, V. K., Severova, E. A., Pominova, N. M., Denisov, Y. D., & Belyaev, N. N. (2016). Миелоидные супрессорные клетки при экспериментальном хроническом воспалении. Вестник КазНУ. Серия биологическая, 67(2), 178–186. извлечено от https://bb.kaznu.kz/index.php/biology/article/view/1193
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