RAPD-fingerprinting of soil micromycetes
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Keywords:
RAPD, primers, micromycetes, fingerprinting,Abstract
In this study stable RAPD-profiles for 7 soil micromycetes isolates were obtained by amplification of genomic DNA with single nonspecific primers. The arbitrary primer (decamer AP3), primer derived from the intergenic spacer region (T3B) and microsatellite primer (GTG)5 were applied in PCR. Precise and reproducible profiles of the amplification products were obtained for all seven micromycetes isolates. Species specificity of the amplicons number and size was demonstrated. In some cases, intraspecific variability was observed. The results show justification for the use of DNA fingerprinting for rapid identification and labeling of soil micromycetes, as well as evaluation of their stability during long-term cultivation.References
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2 Практикум по микробиологии. / Под ред. А.И. Нетрусова – М.: Академия, 2005. – С.119.
3 Fegan M., Manners J.M., Maclean D.J. Irwin J.A., Samuels K.D., Holdom D.G., Li D.P. Random amplified polymorphic DNA markers reveal a high degree of genetic diversity in the entomopathogenic fungus Metarhizium artisopliae var. anisopliae // J. Gen. Microbiol. – 1993. – Vol. 139. – P. 2075-2081.
4 Cambereri E.B., Kinsey J.A. An ultra-fast method of DNA extraction from Neurospora // Fungal Genet. Newsl. – 1993. – Vol. 40. – P. 22–23.
5 Thanos M., G. Schonian, W. Meyer C., Schweinoch, Y. Graser, T. G. Mitchell, W. Presber, H.-J. Tietz. Rapid identification of Candida species by DNA fingerprinting with PCR // J. Clin. Microbiol. – 1996. – Vol. 34. – №3. – P. 615-621.
6 Muniz M. de M., Morais E. Silva Tavares P., Meyer W, Nosanchuk J.D., Zancope-Oliveira R.M. Comparison of different DNAbased methods for molecular typing of Histoplasma capsulatum // Appl. Environ. Microbiol. – 2010. – Vol.76. – №13. – P. 4438–4447.
7 Ramachandran P., Lacher D.W., Pfeiler E.A., Elkins C.A. Development of a tiered multilocus sequence typing scheme for members of the Lactobacillus acidophilus complex // Appl. Environ. Microbiol. – 2013. – Vol. 79. – № 23. – P. 7220-7228.
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Li, K. G., Moldagulova, A. K., Bekenova, E. E., Kazybaev, A. K., Kairova, M. Z., Urazova, M. S., Shajhin, S. M., & Almagambetov, K. H. (2015). RAPD-fingerprinting of soil micromycetes. Experimental Biology, 60(1), 34–37. Retrieved from https://bb.kaznu.kz/index.php/biology/article/view/51
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Bioinformatics, Genomics and Proteomics. Physical-chemical Biology
