КЛОНИРОВАНИЕ И ЭКСПРЕССИЯ кДНК ЭНДО-Β-1,4-ГЛЮКОНАЗЫ ГРИБА ASPERGILLUS NIGER В E.COLI
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Keywords:
vector pET11d, EG enzymes,Abstract
EG enzymes are key components in fungal cellulase systems, and their functional activity is critical for hydrolysis of cellulose. A gene encoding endogluconase was successfully cloned in E. coli. The method of clonig included amplification of cDNA using the gene specific primer by PCR. The PCR product was cloned into the vector pET11d plasmid under the control of T7 promoter. It was shown gene expression of eng1 in recombinant strain of E. coli.References
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Тастанова, А. С., Акбасова, А. Ж., Тайпакова, С. М., & Бисенбаев, А. К. (2015). КЛОНИРОВАНИЕ И ЭКСПРЕССИЯ кДНК ЭНДО-Β-1,4-ГЛЮКОНАЗЫ ГРИБА ASPERGILLUS NIGER В E.COLI. Experimental Biology, 49(3), 136–137. Retrieved from https://bb.kaznu.kz/index.php/biology/article/view/509
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BIOLOGY
