Application of molecular methods to identify lactic acid bacteria
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Keywords:
RAPD-PCR analysis, primers, lactic acid bacteria, fingerprints.Abstract
DNA polymorphisms of isolates of lactic acid bacteria (LAB) were analyzed by RAPD - PCR. Primer derived from the intergenic spacer region (T3B) and microsatellite primer (GTG)5 were used as a single arbitrary primer. Laws of formation of characteristic bands in agarose gel after electrophoresis (fingerprints) give reasons for their use in the identification of the LAB. Comparison of species specific RAPD-PCR fingerprints belonging to the LAB isolates from food, with fingerprints of reference strains will allow for identification the microorganisms at species level, even if they are not revealed by standard biochemical methods. RAPD-PCR analysis is good for molecular typing of LAB in thelaboratories with relatively limited technical equipment.References
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2 Kairova M., Moldagulova A. Isolation and molecular identification of lactic acid bacteria from horsemeat and homemade sour cream // J. World academy of Science, Engineering and Technology.Dubai. - 2013. – Vol.73. - P. 1220-1225.
3 Thanos M., G. Schonian, W. Meyer C., Schweinoch, Y. Graser, T. G. Mitchell, W. Presber, H.-J. Tietz. Rapid identification of Candida species by DNA fingerprinting with PCR // J. Clin. Microbiol. – 1996. – Vol. 34. – №3. – P. 615–621.
4 Wongsawad C., Wongsawad P., Chai J.Y., Anuntalabhochai S. Haplorchis taichui, Witenberg, 1930: Development of a HAT-RAPD marker for the detection of minute intestinal fluke infection // Experimental Parasitology. – 2009. - Vol. 123(2). – P. 158-161.
5 Wongsawad P. and Wongsawad C. DNA Fingerprints Of Some Heterophyid Trematodes From Adult and Metacercarial Stages In Thailand // Southeast Asian J Trop Med Public Health. - 2007. – Vol. 38. – P. 110–114.
6 Guang-Hui Du, Zhi-Qiang Zhang, Qing-Jun Li. Morphological And Molecular Evidence for Natural Hybridization In Sympatric Population Of Roscoea Humeana and R. Cautleoides (Zingiberaceae) // J Plant Res. – 2012. – Vol.125. – P. 595- 603.
7 Wongsawad C. Detection of S. Falcatus Using Hat-RAPD PCR // Southeast Asian J Trop Med Public Health. – 2011. – Vol. 42. – P. 46-52.
8 Welsh J.,. Pretzman C., Postic D., Saint Girons I., Baranton G., McClelland M. Genomic fingerprinting by arbitrarily primed polymerase chain reaction resolves Borrelia burgdorferi into three distinct phyletic groups // Int. J. Syst. Bacteriol. -1992. – Vol. 42. – Р. 370–377.
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Shaikhin, S. M., Li, K. G., Moldagulova, A. K., Bekenova, E. E., Kazhybaev, A. K., Kairova, M. J., Urazova, M. S., & Almagambetov, K. H. (2015). Application of molecular methods to identify lactic acid bacteria. Experimental Biology, 60(2), 395–397. Retrieved from https://bb.kaznu.kz/index.php/biology/article/view/983
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