Obtaining genetically modified tobacco plant Nicotiana tabacum with increased tolerance to low temperatures
57 23
Keywords:
stressful condition, low temperature, tobacc, gene of transcription factor AtDREB1A, transformation.Abstract
In this investigation transformation of tobacco plants Nicotiana tabacum was carried out using аgrobacterium strains contained protein-encoding DNA segment of transcription factor AtDREB1A gene from Arabidopsis thaliana. The presence of the target gene was proved by the obtaining of fragment with expected length after the isolation of DNA from regenerated plants leaves and by positive answer after reverse transcription reaction (RT) using RNA isolated from leaf disks. Several transgenic lines demonstrated increased tolerance to low temperatures.References
1 Bartels D., Sunkar R. Drought and salt tolerance in plants // Critical Reviews in Plant Science. - 2005. – V. 21. P, 1–36.
2 Bray E.A., Plant responses to water deficit // Trends Plant Sci. - 1997. – V. 2. – P, 48–54.
3 Chaves M.M., Maroco J.P., Pereira J.S., Understanding plant responses to drought —from genes to the whole plant // Funct. Plant Biol. - 2003. – V. 30. – P, 239–264.
4 Munns R., Tester M., Mechanisms of salinity tolerance // Annu. Rev. Plant Biol. - 2008. V. 59. – P, 651-681
5 Witcombe J.R., Hollington P.A., Howarth C.J., Reader S., Steele K.A., Breeding for abiotic stresses for sustainable agriculture // Philos. Trans. R. Soc. B. - 2008. V. 363. – P, 703–716.
6 Charu Lata and Manoj Prasad. Role of DREBs in regulation of abiotic stress responses in plants // Journal of Experimental Botany. - 2011. V.11. – P, 111-118.
7 Kratsch H.A., Wise R.R. The ultrastructure of chilling stress // Plant Cell Environ. - 2000. – V. 23. – P, 337– 350.
8 Hasegawa P.M., Bressan R.A., Zhu J.K., Bohnert H.J. Plant cellular and molecular responses to high salinity //Annu. Rev. Plant Physiol. Plant Mol. Biol. - 2000. V. 51. – P, 463–499.
9 Strizhov N., Abraham E., Okresz L. et al. Differential expression of two 1-pyrroline5carboxylate synthetase genes controlling proline accumulation during salt stress requires ABA and is regulated by ABA1, ABI1 and AXR2 in Arabidopsis // Plant J. - 1997. V. 12. – Р, 557–569.
10 Ahuja I., R.C.H. de Vos, Bones A.M., Hall R.D., Plant molecular stress responses face climate change // Trends Plant Sci. - 2010. V. 15. – P, 664–674.
11 Chinnusamy V., Schumaker K., Zhu J. Molecular genetic perspectives on cross-talk and specificity in abiotic stress signalling in plants // Journal of Experimental Botany. - 2004. V. 55. – P, 225–236.
12 Maria Reguera, Zvi Peleg, Eduardo Blumwald. Targeting metabolic pathways for genetic engineering abiotic stress-tolerance in crops // Biochimica et Biophysica Acta. - 2011. V. 11. – P, 101-109.
13 Junya Mizoi, Kazuo Shinozaki, Kazuko Yamaguchi-Shinozaki. AP2/ERF family transcription factors in plant abiotic stress responses // Biochimica et Biophysica Acta. - 2012. V.12. – P.86-96.
14 Saleh A. and M. Pagés. Plant AP2/ERF transcription factors // Genetika. - 2003. V. 35, No. 01. – P, 37-50. 2003
15 Карпова О.В., Ерискина Е.А., Станбекова Г.Э., Жигайлов А.В., Кожанов Е.В., Полимбетова Н.С., Искаков Б.К. Клонирование и экспрессия гена транскрипционного фактора AtDREB1А из Arabidopsis thaliana в условиях in vitro // Устойчивость организмов к неблагоприятным факторам внешней среды. Материалы Всероссий-
ской научной конференции. Иркутск. - 2009. - №1. – С. 207-210.
2 Bray E.A., Plant responses to water deficit // Trends Plant Sci. - 1997. – V. 2. – P, 48–54.
3 Chaves M.M., Maroco J.P., Pereira J.S., Understanding plant responses to drought —from genes to the whole plant // Funct. Plant Biol. - 2003. – V. 30. – P, 239–264.
4 Munns R., Tester M., Mechanisms of salinity tolerance // Annu. Rev. Plant Biol. - 2008. V. 59. – P, 651-681
5 Witcombe J.R., Hollington P.A., Howarth C.J., Reader S., Steele K.A., Breeding for abiotic stresses for sustainable agriculture // Philos. Trans. R. Soc. B. - 2008. V. 363. – P, 703–716.
6 Charu Lata and Manoj Prasad. Role of DREBs in regulation of abiotic stress responses in plants // Journal of Experimental Botany. - 2011. V.11. – P, 111-118.
7 Kratsch H.A., Wise R.R. The ultrastructure of chilling stress // Plant Cell Environ. - 2000. – V. 23. – P, 337– 350.
8 Hasegawa P.M., Bressan R.A., Zhu J.K., Bohnert H.J. Plant cellular and molecular responses to high salinity //Annu. Rev. Plant Physiol. Plant Mol. Biol. - 2000. V. 51. – P, 463–499.
9 Strizhov N., Abraham E., Okresz L. et al. Differential expression of two 1-pyrroline5carboxylate synthetase genes controlling proline accumulation during salt stress requires ABA and is regulated by ABA1, ABI1 and AXR2 in Arabidopsis // Plant J. - 1997. V. 12. – Р, 557–569.
10 Ahuja I., R.C.H. de Vos, Bones A.M., Hall R.D., Plant molecular stress responses face climate change // Trends Plant Sci. - 2010. V. 15. – P, 664–674.
11 Chinnusamy V., Schumaker K., Zhu J. Molecular genetic perspectives on cross-talk and specificity in abiotic stress signalling in plants // Journal of Experimental Botany. - 2004. V. 55. – P, 225–236.
12 Maria Reguera, Zvi Peleg, Eduardo Blumwald. Targeting metabolic pathways for genetic engineering abiotic stress-tolerance in crops // Biochimica et Biophysica Acta. - 2011. V. 11. – P, 101-109.
13 Junya Mizoi, Kazuo Shinozaki, Kazuko Yamaguchi-Shinozaki. AP2/ERF family transcription factors in plant abiotic stress responses // Biochimica et Biophysica Acta. - 2012. V.12. – P.86-96.
14 Saleh A. and M. Pagés. Plant AP2/ERF transcription factors // Genetika. - 2003. V. 35, No. 01. – P, 37-50. 2003
15 Карпова О.В., Ерискина Е.А., Станбекова Г.Э., Жигайлов А.В., Кожанов Е.В., Полимбетова Н.С., Искаков Б.К. Клонирование и экспрессия гена транскрипционного фактора AtDREB1А из Arabidopsis thaliana в условиях in vitro // Устойчивость организмов к неблагоприятным факторам внешней среды. Материалы Всероссий-
ской научной конференции. Иркутск. - 2009. - №1. – С. 207-210.
Downloads
How to Cite
Shadymova, Y. A., Pisarenko, A. M., Nargilova, R. M., Karpova, O. V., & Iskakov, B. K. (2015). Obtaining genetically modified tobacco plant Nicotiana tabacum with increased tolerance to low temperatures. Experimental Biology, 60(2), 391–394. Retrieved from https://bb.kaznu.kz/index.php/biology/article/view/982
Issue
Section
BOTANY
