Получение трансгенных растений табака, экспрессирующих транскрипционный фактор AtDREB2A. AtDREB2A транскрипциялық факторын экспрессиялайтын трансгендік темекі өсімдіктерін алу.
Ключевые слова:
фактор транскрипции, AtDREB2A, засуха, засоление, табак, транскрипция факторы, құрғақшылық, тұздану, темекі,Аннотация
Целью работы является получение трансгенных растений табака, экспрессирующих транскрипционный фактор AtDREB2A в нативной и мутированной формах, для изучения молекулярных механизмов устойчивости растений к засухе. Амплифицирован фрагмент ДНК, кодирующий ген транскрипционного фактора AtDREB2A из Arabidopsis thaliana, и проведен делеционный мутагенез кодирующей последовательности с целью получения конститутивно активного белка. Обе последовательности клонированы под контроль конститутивного 35S-промотора вируса CaMV и индуцибельного промотора rd29A, а также различных вариантов усилителей трансляции.Получены трансгенные растения табака, несущие в своем геноме кодирующую последовательность транскрипционного фактора AtDREB2A в нативной и мутированной формах. Жұмыс мақсаты – өсімдіктердің құрғақшылыққа төзуінің молекулалық тетіктерін зерттеу үшін AtDREB2A транскрипциялық факторын табиғи және мутант түрде экспрессиялайтын трансгендік темекі өсімдіктерін алу. Arabidopsis thaliana-ның AtDREB2A транскрипциялық факторын кодтайтын геннің ДНК бөлшегі көбейтілді, және кодтайтын тізбекке конститутивті әрекетті белок алу үшін делециялық мутагенез жасалды. Екі тізбек CaMV вирусының конститутивті 35S-промоторының және индуцибельді rd29A промотордың,сондай-ақ әртүрлі трансляция күшейткіштерінің бақылауында клондалды. AtDREB2A транскрипциялық факторын табиғи және мутант түрде өз геномында сақтайтын трансгендік темекі өсімдіктері алынды.Библиографические ссылки
1 Bray E.A. Responses to abiotic stresses. //Biochemistry and molecular biology of plants. – American Society of
Plant Physiologists, 2000. - P.1158-249.
2 Rodríguez M. et al. Molecular aspects of abiotic stress in plants. //Biotecnología Aplicada. – 2005. – Vol. 22. - № 1.
– P. 1-10.
3 Seki M. Monitoring the expression profiles of 7000 Arabidopsis genes under drought, cold and high salinity stresses using a full-length cDNA microarray // The Plant Journal. – 2002. - №31. – P. 279–292.
4 Bohnert H. J. et al. A genomics approach towards salt stress tolerance // Plant Physiol. Biochem. – 2001. - №39. – P.295-311.
5 Chen W. Expression profile matrix of Arabidopsis transcription factor genes suggests their putative functions in response to environmental stresses // Plant Cell. – 2002. - №14. - № – P. 559-74.
6 Fowler S. Arabidopsis transcriptome pro-filing indicates that multiple regulatory pathways are activated during cold
acclimation in addition to the CBF cold response pathway // Plant Cell. – 2002. - №14. – P. 1675-90.
7 Abdelaty S. Plant AP2/ERF transcription factors // Genetika. – 2003. - Vol. 35. - №1. – P. 37-50.
8 Qiang L. Two Transcription Factors, DREB1 and DREB2, with an EREBP/AP2 DNA Binding Domain Separate
Two Cellular Signal Transduction Pathways in Drought- and Low-Temperature-Responsive Gene Expression, Respectively, in Arabidopsis // The Plant Cell. – 1998. – Vol. 10. – P. 1391–1406.
9 Hsieh T.H. Heterology expression of the Arabidopsis C-repeat/ dehydration response element binding factor 1 gene
confers elevated tolerance to chilling and oxidative stresses in transgenic tomato // Plant Physiol. – 2002. - №129. – P.
1086-94.
10 Agarwal P.K.et al. Role of DREB transcription factors in abiotic and biotic stress tolerance in plants // Plant Cell. –
2006. - №25. – P. 1263–1274.
11 Shi-Qing Gao et al. A cotton (Gossypium hirsutum) DRE-binding transcription factor gene, GhDREB, confers enhanced tolerance to drought, high salt, and freezing stresses in transgenic wheat // Plant Cell Rep. – 2009. - №28. - P.301–311.
12 Sakuma Y. et al. Functional analysis of an Arabidopsis transcription factor, DREB2A, involved in droughtresponsive
gene expression // Plant Cell. – 2006. - №18. – P. 1292–1309.
13 Qin F. Arabidopsis DREB2A-interacting proteins function as RING E3 ligases and negatively regulate plant drought
stress-responsive gene expression // Plant Cell. – 2008. – Vol. 20. – P. 1693-1707.
14 Agarwal P. et al. Stress-inducible DREB2A transcription factor from Pennisetum glaucum is a phosphoprotein and
its phosphorylation negatively regulates its DNA-binding activity // Mol. Genet. Genomics. – 2007. – Vol. 277 – P.
189–198.
15 Писаренко, Наргилова и др. Получение растений картофеля, экспрессирующих транскрипционный фактор
AtDREB2A //Вестник КазНУ. Серия биологическая. – 2013. - №3/1 (59). – С. 164-168
Plant Physiologists, 2000. - P.1158-249.
2 Rodríguez M. et al. Molecular aspects of abiotic stress in plants. //Biotecnología Aplicada. – 2005. – Vol. 22. - № 1.
– P. 1-10.
3 Seki M. Monitoring the expression profiles of 7000 Arabidopsis genes under drought, cold and high salinity stresses using a full-length cDNA microarray // The Plant Journal. – 2002. - №31. – P. 279–292.
4 Bohnert H. J. et al. A genomics approach towards salt stress tolerance // Plant Physiol. Biochem. – 2001. - №39. – P.295-311.
5 Chen W. Expression profile matrix of Arabidopsis transcription factor genes suggests their putative functions in response to environmental stresses // Plant Cell. – 2002. - №14. - № – P. 559-74.
6 Fowler S. Arabidopsis transcriptome pro-filing indicates that multiple regulatory pathways are activated during cold
acclimation in addition to the CBF cold response pathway // Plant Cell. – 2002. - №14. – P. 1675-90.
7 Abdelaty S. Plant AP2/ERF transcription factors // Genetika. – 2003. - Vol. 35. - №1. – P. 37-50.
8 Qiang L. Two Transcription Factors, DREB1 and DREB2, with an EREBP/AP2 DNA Binding Domain Separate
Two Cellular Signal Transduction Pathways in Drought- and Low-Temperature-Responsive Gene Expression, Respectively, in Arabidopsis // The Plant Cell. – 1998. – Vol. 10. – P. 1391–1406.
9 Hsieh T.H. Heterology expression of the Arabidopsis C-repeat/ dehydration response element binding factor 1 gene
confers elevated tolerance to chilling and oxidative stresses in transgenic tomato // Plant Physiol. – 2002. - №129. – P.
1086-94.
10 Agarwal P.K.et al. Role of DREB transcription factors in abiotic and biotic stress tolerance in plants // Plant Cell. –
2006. - №25. – P. 1263–1274.
11 Shi-Qing Gao et al. A cotton (Gossypium hirsutum) DRE-binding transcription factor gene, GhDREB, confers enhanced tolerance to drought, high salt, and freezing stresses in transgenic wheat // Plant Cell Rep. – 2009. - №28. - P.301–311.
12 Sakuma Y. et al. Functional analysis of an Arabidopsis transcription factor, DREB2A, involved in droughtresponsive
gene expression // Plant Cell. – 2006. - №18. – P. 1292–1309.
13 Qin F. Arabidopsis DREB2A-interacting proteins function as RING E3 ligases and negatively regulate plant drought
stress-responsive gene expression // Plant Cell. – 2008. – Vol. 20. – P. 1693-1707.
14 Agarwal P. et al. Stress-inducible DREB2A transcription factor from Pennisetum glaucum is a phosphoprotein and
its phosphorylation negatively regulates its DNA-binding activity // Mol. Genet. Genomics. – 2007. – Vol. 277 – P.
189–198.
15 Писаренко, Наргилова и др. Получение растений картофеля, экспрессирующих транскрипционный фактор
AtDREB2A //Вестник КазНУ. Серия биологическая. – 2013. - №3/1 (59). – С. 164-168
Загрузки
Выпуск
Раздел
БИОТЕХНОЛОГИЯ
