Obtaining transgenic tobacco plants expressed transcription factor AtDREB2A gene.

Abstract

The task of this work is obtaining of transgenic tobacco plants, expressed transcription factor AtDREB2A gene in native and mutant forms for investigation of molecular mechanisms of plant resistance to drought. DNA-fragment coding transcription factor AtDREB2A gene from Arabidopsis thaliana was amplified and deletion mutagenesis of coding sequence was carried out with the view of obtaining constitutively active protein. Both of sequences were cloned under control of 35S-promoter CaMV or inducible rd29A promoter and also different variants of translation enhancers. The transgenic plants consisting coding sequences of transcription factor AtDREB2A in native and mutant forms in its genome were gained.