Formation of a collection of wheat to search for new alleles genome

Authors

  • O. N. Khapilina РГП «Национальный центр биотехнологии» КН МОН РК
  • M. Zh. Kairova РГП «Национальный центр биотехнологии» КН МОН РК
  • R. N. Kalendar Institute of Biotechnology MTT/BI Plant Genomics Lab
        37 46

Keywords:

wheat, polymorphism, collection, sequencing, DNA, gene, molecular genetic markers, isozymes,

Abstract

The problem of the conservation of biological diversity at the level of genes is very important. One of the primary problems is the choice of approaches techniques and criteria for assessing genetic diversity. Priority areas include the development of science technology analysis of plant genomes. One of the important aspects of genetics and plant breeding practices in detail the material used. To solve this problem, introducing new technologies, which are based on the analysis of DNA polymorphism. The most important thing in this kind of research is the formation of representative collections containing all allelic variants and gene combinations. Formation of a collection of wheat, which is the aim of the research, was conducted to identify the genetic forms of the test species.

References

1 Митрофанова О.П. Генетические ресурсы пшеницы в России: состояние и предселекционное изучение // Вавиловский журнал генетики и селекции. – 2012. – Т. 16, № 1. – С. 10 – 20.

2 Paux E., Sourdille P., Salse J., Saintenac C., Choulet F., Leroy P., Korol A., Michalak M., Kianian S., Spielmeyer W., Lagudah E.,Somers D., Kilian A., Alaux M., Vautrin S., Berges H., Eversole K., Appels R., Safar J. A physical map of the 1-gigabase bread wheat chromosome 3B // Science. – 2008. – Vol. 322. – P. 101 – 104.

3 Rabinowicz P.D., Citek R., Budiman M.A., Nunberg A., Bedell J.A., Lakey N., O’Shaughnessy A.L., Nascimento L.U., Mc-Combie W.R., Martienssen R.A. Differential methylation of genes and repeats in land plants // Genome Res. – 2005. – Vol. 15. – P.1431– 1440.

4 Berkman P.J., Skarshewski A., Lorenc M.T., Lai K., Duran C., Ling E.Y..S, Stiller J., Smits L., Imelfort M., Manoli S., McKenzie M., Kubalakova M., Simkova H., Batley J., Fleury D., Dolezel J., Edwards D. Sequencing and assembly of low copy and genic regions of isolated Triticum aestivum chromosome arm 7DS// Plant Biotech J. – 2011. – Vol. 9. – P. 768 – 775.

5 Wanjugi H., Coleman-Derr D., Huo N.X., Kianian S.F., Luo M.C., Wu J.J., Anderson O, Gu Y.Q. Rapid development of PCRbased genome-specific repetitive DNA junction markers in wheat // Genome. – 2009. – Vol. 52. – P.576 – 587.

6 http://urgi.versailles.inra.fr/gb2/gbrowse/wheat_phys_pub/

7 Гончаров П.Л. Генофонд сельскохозяйственных культур для селекции устойчивых сортов. – Новосибирск, 1999.– С. 3–6.

8 Scanolalios J. G., Chao S.E. Melville J. C. Biochemical characterization of the major amylase form coded by the Amy -1 gene in maisze // Nature biotechnology. – 1978. – Vol. 69. – P.149 – 154.

Downloads

How to Cite

Khapilina, O. N., Kairova, M. Z., & Kalendar, R. N. (2015). Formation of a collection of wheat to search for new alleles genome. Experimental Biology, 60(1), 56–59. Retrieved from https://bb.kaznu.kz/index.php/biology/article/view/56

Issue

Section

Bioinformatics, Genomics and Proteomics. Physical-chemical Biology