Binding sites of miRNAs with transcription factors genes of CAMELUS FERUS
Keywords:
mRNA, miRNA, transcription factors, Camelus ferus.Abstract
We searched 239 miRNAs binding sites in mRNAs of 63 from 157 transcription factors genes of Camelus ferus Zinc finger family. 19 binding sites are located in the 5'UTRs, 64 sites are located in the CDSs and 156 sites are located in the 3'UTRs. The mRNA of GLI2 gene has binding sites for eight miRNAs. The ΔG/ΔGm value changed from 90 to 100 %. miR-1322 has one binding site in GLI1, HINFP, HIVEP1, MTF1, SALL4, SP1, ZNF335 and ZNF451 genes, two sites in ZNF142, three sites in EGR1 gene. The ΔG value varied from -114,6 kJ/mole to -138,0 kJ/mole. Some miRNAs with a length of 23 and 24 nucleotides also have a high value ΔG varied from -112,5 kJ/mole to -129,5 kJ/mole. The results show a strong interaction between the expression of genes of transcription factors and miRNAs.References
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2. Kaczensky P., Adiya Y., Von Wehrden H., Mijiddorj B., Walzer C., et al. Space and habitat use by wild camels in the Transaltai Gobi of Southern Mongolia // Biol. Conservation. - 2014. - Vol. 169. - P. 311-318.
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5. Huang L., Walter V., Hayes D.N., Onaitis M. Hedgehog-GLI signaling inhibition suppresses tumor growth in squamous lung cancer // Clin Cancer Res. - 2014. - Vol. 20(6). - P. 1566-1575.
6. Wang G., Jiang L., Song J., Zhou SF., Zhang H., et al. Mipu1 protects H9c2 myogenic cells from hydrogen peroxide-induced apoptosis through inhibition of the expression of the death receptor Fas // Int J Mol Sci. - 2014. - Vol. 15(10). - P. 18206-18220.
7. Gizard F., Robillard R., Barbier O., Quatannens B., Faucompré A., et al. TReP-132 controls cell proliferation by regulating the expression of the cyclin-dependent kinase inhibitors p21WAF1/Cip1 and p27Kip1 // Mol Cell Biol. - 2005. - Vol. 25(11). - P. 4335-4348.
8. Funke B., Puech A., Saint-Jore B., Pandita R., Skoultchi A., et al. Isolation and characterization of a human gene containing a nuclear localization signal from the critical region for velo-cardio-facial syndrome on 22q11 // Genomics. - 1998. - Vol. 53(2). - P.146-154.
9. Nomura N., Zhao M.J., Nagase T., Maekawa T., Ishizaki R., et al. HIV-EP2, a new member of the gene family encoding the human immunodeficiency virus type 1 enhancer-binding protein. Comparison with HIV-EP1/PRDII-BF1/MBP-1 // Biol Chem. - 1991. - Vol. 5. - P. 4.
10. Bard-Chapeau E.A., Gunaratne J., Kumar P., Chua B.Q., Muller J., et al. EVI1 oncoprotein interacts with a large and complex network of proteins and integrates signals through protein phosphorylation // Proc Natl Acad Sci USA. - 2013. - Vol. 30. - P. 2885-2894.
11. Yang H.Y., Kim S.H., Kim S.H., Kim D.J., Kim S.U., et al. The suppression of zfpm-1 accelerates the erythropoietic differentiation of human CD34+ cells // Biochem Biophys Res Commun. - 2007. - Vol. 353(4). - P. 978-984.
12. Zins K., Pomyje J., Hofer E., Abraham D., Lucas T., et al. Egr-1 upregulates Siva-1 expression and induces cardiac fibroblast apoptosis // Int J Mol Sci. - 2014. - Vol. 15(1). - P. 1538-1553.
13. Sekimata M., Homma Y. Regulation of Rb gene expression by an MBD2-interacting zinc finger protein MIZF during myogenic differentiation // Biochem Biophys Res Commun. - 2004. - Vol. 325(3). - P. 653-659.
14. Kumar R., Selth L.A., Schulz R.B., Tay B.S., Neilsen P.M., et al. Genome-wide mapping of ZNF652 promoter binding sites in breast cancer cells // Cell Biochem. - 2011. - Vol. 112(10) . - P. 2742-2747.
15. Spörl F., Korge S., Jürchott K., Wunderskirchner M., Schellenberg K., et al. Krüppel-like factor 9 is a circadian transcription factor in human epidermis that controls proliferation of keratinocytes // Proc Natl Acad Sci USA. - 2012. - Vol. 109(27). - P. 903-908.
16. Li J., Wang Y., Fan X., Mo X., Wang Z., et al. ZNF307, a novel zinc finger gene suppresses p53 and p21 pathway // Biochem Biophys Res Commun. - 2007. - Vol. 363(4). - P. 895-900.
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Alybaeva, A., Niyazova, R., Faye, B., & Ivashchenko, A. (2016). Binding sites of miRNAs with transcription factors genes of CAMELUS FERUS. Experimental Biology, 65(3), 94–98. Retrieved from https://bb.kaznu.kz/index.php/biology/article/view/1102
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МOLECULAR BIOLOGY AND GENETICS