The effects of low temperature atmospheric plasma on viability of metastatic prostate cancer cells
69 47
Keywords:
metastatic cancer, prostate cancer, low temperature plasma, cell viability.Abstract
The study presents the date of effects of atmospheric pressure low-temperature plasma generated by dielectric barrier discharge on viability and morphological features of DU145 human metastatic prostate cancer cells. The effect of low temperature plasma on cells depends on the dose and exposure time. If lower doses of plasma cause cell proliferation, the highest doses couse death of cancer cells. Thus, in relatively high dose of plasma, i.e. plasma dose №7 (frequency - 250 Hz, power - 2.2 W, and time of exposure - 30 seconds) after 24 and 48 hours post 1 minute incubation of cells with plasma treated saline solution reduced cell viability 47 and 38%, respectively, and in post 10 minutes incubation of cells reduces cell viability 19% and 11%. These results demonstrate the potential clinical use of the proposed method for the treatment of prostate cancer.References
Әдебиеттер
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2. Нұрғазиев Қ.Ш., Сейтқазина Ғ.Ж., Әжмағамбетова Ә.Е., Сейсенбаева Г.Т. Қазақстан Республикасы онкологиялық қызметінің 2013 жылдық көрсеткіштері (статистикалық мәліметтер). – Алматы, 2014. – 108 б.
3. Hail N, Jr., Chen P, Kepa J.J. Selective apoptosis induction by the cancer chemopreventive agent N-(4-hydroxyphenyl)retinamide is achieved by modulating mitochondrial bioenergetics in premalignant and malignant human prostate epithelial cells // Apoptosis : an international journal on programmed cell death. – 2009. – Vol. 14. – № 57. – P. 849-863.
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5. Kalghatgi S., Kelly C.M., Cerchar E., Torabi B., Alekseev O., et al. Effects of Non-Thermal Plasma on Mammalian Cells // PLoS ONE. – 2011. – Vol. 6. – № 1. e16270.
6. Tuhvatulin A.I., Sysolyatina E.V., Scheblyakov D.V., Logunov D.Yu., Vasiliev M.M., Yurova M.A., Danilova M.A., Petrov O.F., et al. Non-thermal Plasma Causes p53-Dependent Apoptosis in Human Colon Carcinoma Cells // Acta Naturae. – 2012. – Vol. 4. – № 3. – P. 82–87.
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8. Jogy I. Characteristics and classification of plasmas // PlasTEP trainings course and Sammer school. – 2011. – P. 1-33
9. Bunu M.S., Sasikala P., Dhanapal A., Kavitha V., Yazhini G., Rajamani L. Cold plasma as a novel food processing technology // International Jornal of Emerging trends in Engineering and Development. – 2012. – Vol. 4. – № 2. – P. 803-818.
10. Алейник А.Н., Байков А.Н., Дамбаев Г.Ц., Денеко О.И., Жданова О.С., Красноженов Е.П., Семичев Е.В. Особенности взаимодействия неравновесной плазмы с живыми тканями // Вестник науки Сибири. – 2012. – Vol. 3. – № 4. – С. 44-48.
11. Fridman G., Peddinghaus M., Ayan H., Fridman A., Balasubramanian M., et al. Blood Coagulation and Living Tissue Sterilization by Floating-Electrode Dielectric Barrier Discharge in Air // Plasma Chemistry and Plasma Processing. – 2006. – Vol.26. – P. 425–442.
12. Fridman G., Shereshevsky A., Jost M.M., Brooks A.D., Fridman A., Gutsol A., et al. Floating Electrode Dielectric Barrier Discharge Plasma in Air Promoting Apoptotic Behavior in Melanoma Skin Cancer Cell Lines // Plasma Chemistry and Plasma Processing. – 2007. – Vol. 27. – № 2. – P. 163–176.
13. Panngom K., Baik K.Y., Nam M.K., Han J.H., Rhim H., Choi E.H. Preferential killing of human lung cancer cell lines with mitochondrial dysfunction by nonthermal dielectric barrier discharge plasma // Cell Death Dis. – 2013. – Vol. 4. – № 5. – e642.
14. Panngom K., Lee S.H., Park D.H., Sim G.B., Kim Y.H., Uhm H.S. Non-Thermal Plasma Treatment Diminishes Fungal Viability and Up-Regulates Resistance Genes in a Plant Host // PLoS One. – 2014. – Vol. 9. – № 6. – e99300.
References
1. Jemal A., Siegel R., Xu J., Ward E. Cancer statistics, 2010 // CA: a cancer journal for clinicians. – 2010. – Vol. 60. – № 5. – P. 277-300.
2. Nұrғaziev Қ.Sh., Sejtқazina Ғ.Zh., Әzhmaғambetova Ә.E., Sejsenbaeva G.T. Қazaқstan Respublikasy onkologijalyқ қyzmetіnің 2013 zhyldyқ kөrsetkіshterі (statistikalyқ mәlіmetter). – Almaty, 2014. – 108 b.
3. Hail N, Jr., Chen P, Kepa J.J. Selective apoptosis induction by the cancer chemopreventive agent N-(4-hydroxyphenyl)retinamide is achieved by modulating mitochondrial bioenergetics in premalignant and malignant human prostate epithelial cells // Apoptosis : an international journal on programmed cell death. – 2009. – Vol. 14. – № 57. – P. 849-863.
4. Ben Sahra I, et al. Targeting cancer cell metabolism: the combination of metformin and 2-deoxyglucose induces p53-dependent apoptosis in prostate cancer cells // Cancer research. –2010. – Vol. 70. – №6. – P.2465-2475.
5. Kalghatgi S., Kelly C.M., Cerchar E., Torabi B., Alekseev O., et al. Effects of Non-Thermal Plasma on Mammalian Cells // PLoS ONE. – 2011. – Vol. 6. – № 1. e16270.
6. Tuhvatulin A.I., Sysolyatina E.V., Scheblyakov D.V., Logunov D.Yu., Vasiliev M.M., Yurova M.A., Danilova M.A., Petrov O.F., et al. Non-thermal Plasma Causes p53-Dependent Apoptosis in Human Colon Carcinoma Cells // Acta Naturae. – 2012. – Vol. 4. – № 3. – P. 82–87.
7. Nehra V., Kumar A., Dwivedi H.K. Atmospheric non-thermal plasma sources // International Journal of Engineering. – 2008. – Vol. 2. – № 1. – P. 53-68.
8. Jogy I. Characteristics and classification of plasmas // PlasTEP trainings course and Sammer school. – 2011. – P. 1-33
9. Bunu M.S., Sasikala P., Dhanapal A., Kavitha V., Yazhini G., Rajamani L. Cold plasma as a novel food processing technology // International Jornal of Emerging trends in Engineering and Development. – 2012. – Vol. 4. – № 2. – P. 803-818.
10. Alejnik A.N., Bajkov A.N., Dambaev G.C., Deneko O.I., Zhdanova O.S., Krasnozhenov E.P., Semichev E.V. Osobennosti vzaimodejstvija neravnovesnoj plazmy s zhivymi tkanjami // Vestnik nauki Sibiri. – 2012. – Vol. 3. – № 4. – S. 44-48.
11. Fridman G., Peddinghaus M., Ayan H., Fridman A., Balasubramanian M., et al. Blood Coagulation and Living Tissue Sterilization by Floating-Electrode Dielectric Barrier Discharge in Air // Plasma Chemistry and Plasma Processing. – 2006. – Vol.26. – P. 425–442.
12. Fridman G., Shereshevsky A., Jost M.M., Brooks A.D., Fridman A., Gutsol A., et al. Floating Electrode Dielectric Barrier Discharge Plasma in Air Promoting Apoptotic Behavior in Melanoma Skin Cancer Cell Lines // Plasma Chemistry and Plasma Processing. – 2007. – Vol. 27. – № 2. – P. 163–176.
13. Panngom K., Baik K.Y., Nam M.K., Han J.H., Rhim H., Choi E.H. Preferential killing of human lung cancer cell lines with mitochondrial dysfunction by nonthermal dielectric barrier discharge plasma // Cell Death Dis. – 2013. – Vol. 4. – № 5. – e642.
14. Panngom K., Lee S.H., Park D.H., Sim G.B., Kim Y.H., Uhm H.S. Non-Thermal Plasma Treatment Diminishes Fungal Viability and Up-Regulates Resistance Genes in a Plant Host // PLoS One. – 2014. – Vol. 9. – № 6. – e99300.
1. Jemal A., Siegel R., Xu J., Ward E. Cancer statistics, 2010 // CA: a cancer journal for clinicians. – 2010. – Vol. 60. – № 5. – P. 277-300.
2. Нұрғазиев Қ.Ш., Сейтқазина Ғ.Ж., Әжмағамбетова Ә.Е., Сейсенбаева Г.Т. Қазақстан Республикасы онкологиялық қызметінің 2013 жылдық көрсеткіштері (статистикалық мәліметтер). – Алматы, 2014. – 108 б.
3. Hail N, Jr., Chen P, Kepa J.J. Selective apoptosis induction by the cancer chemopreventive agent N-(4-hydroxyphenyl)retinamide is achieved by modulating mitochondrial bioenergetics in premalignant and malignant human prostate epithelial cells // Apoptosis : an international journal on programmed cell death. – 2009. – Vol. 14. – № 57. – P. 849-863.
4. Ben Sahra I, et al. Targeting cancer cell metabolism: the combination of metformin and 2-deoxyglucose induces p53-dependent apoptosis in prostate cancer cells // Cancer research. –2010. – Vol. 70. – №6. – P.2465-2475.
5. Kalghatgi S., Kelly C.M., Cerchar E., Torabi B., Alekseev O., et al. Effects of Non-Thermal Plasma on Mammalian Cells // PLoS ONE. – 2011. – Vol. 6. – № 1. e16270.
6. Tuhvatulin A.I., Sysolyatina E.V., Scheblyakov D.V., Logunov D.Yu., Vasiliev M.M., Yurova M.A., Danilova M.A., Petrov O.F., et al. Non-thermal Plasma Causes p53-Dependent Apoptosis in Human Colon Carcinoma Cells // Acta Naturae. – 2012. – Vol. 4. – № 3. – P. 82–87.
7. Nehra V., Kumar A., Dwivedi H.K. Atmospheric non-thermal plasma sources // International Journal of Engineering. – 2008. – Vol. 2. – № 1. – P. 53-68.
8. Jogy I. Characteristics and classification of plasmas // PlasTEP trainings course and Sammer school. – 2011. – P. 1-33
9. Bunu M.S., Sasikala P., Dhanapal A., Kavitha V., Yazhini G., Rajamani L. Cold plasma as a novel food processing technology // International Jornal of Emerging trends in Engineering and Development. – 2012. – Vol. 4. – № 2. – P. 803-818.
10. Алейник А.Н., Байков А.Н., Дамбаев Г.Ц., Денеко О.И., Жданова О.С., Красноженов Е.П., Семичев Е.В. Особенности взаимодействия неравновесной плазмы с живыми тканями // Вестник науки Сибири. – 2012. – Vol. 3. – № 4. – С. 44-48.
11. Fridman G., Peddinghaus M., Ayan H., Fridman A., Balasubramanian M., et al. Blood Coagulation and Living Tissue Sterilization by Floating-Electrode Dielectric Barrier Discharge in Air // Plasma Chemistry and Plasma Processing. – 2006. – Vol.26. – P. 425–442.
12. Fridman G., Shereshevsky A., Jost M.M., Brooks A.D., Fridman A., Gutsol A., et al. Floating Electrode Dielectric Barrier Discharge Plasma in Air Promoting Apoptotic Behavior in Melanoma Skin Cancer Cell Lines // Plasma Chemistry and Plasma Processing. – 2007. – Vol. 27. – № 2. – P. 163–176.
13. Panngom K., Baik K.Y., Nam M.K., Han J.H., Rhim H., Choi E.H. Preferential killing of human lung cancer cell lines with mitochondrial dysfunction by nonthermal dielectric barrier discharge plasma // Cell Death Dis. – 2013. – Vol. 4. – № 5. – e642.
14. Panngom K., Lee S.H., Park D.H., Sim G.B., Kim Y.H., Uhm H.S. Non-Thermal Plasma Treatment Diminishes Fungal Viability and Up-Regulates Resistance Genes in a Plant Host // PLoS One. – 2014. – Vol. 9. – № 6. – e99300.
References
1. Jemal A., Siegel R., Xu J., Ward E. Cancer statistics, 2010 // CA: a cancer journal for clinicians. – 2010. – Vol. 60. – № 5. – P. 277-300.
2. Nұrғaziev Қ.Sh., Sejtқazina Ғ.Zh., Әzhmaғambetova Ә.E., Sejsenbaeva G.T. Қazaқstan Respublikasy onkologijalyқ қyzmetіnің 2013 zhyldyқ kөrsetkіshterі (statistikalyқ mәlіmetter). – Almaty, 2014. – 108 b.
3. Hail N, Jr., Chen P, Kepa J.J. Selective apoptosis induction by the cancer chemopreventive agent N-(4-hydroxyphenyl)retinamide is achieved by modulating mitochondrial bioenergetics in premalignant and malignant human prostate epithelial cells // Apoptosis : an international journal on programmed cell death. – 2009. – Vol. 14. – № 57. – P. 849-863.
4. Ben Sahra I, et al. Targeting cancer cell metabolism: the combination of metformin and 2-deoxyglucose induces p53-dependent apoptosis in prostate cancer cells // Cancer research. –2010. – Vol. 70. – №6. – P.2465-2475.
5. Kalghatgi S., Kelly C.M., Cerchar E., Torabi B., Alekseev O., et al. Effects of Non-Thermal Plasma on Mammalian Cells // PLoS ONE. – 2011. – Vol. 6. – № 1. e16270.
6. Tuhvatulin A.I., Sysolyatina E.V., Scheblyakov D.V., Logunov D.Yu., Vasiliev M.M., Yurova M.A., Danilova M.A., Petrov O.F., et al. Non-thermal Plasma Causes p53-Dependent Apoptosis in Human Colon Carcinoma Cells // Acta Naturae. – 2012. – Vol. 4. – № 3. – P. 82–87.
7. Nehra V., Kumar A., Dwivedi H.K. Atmospheric non-thermal plasma sources // International Journal of Engineering. – 2008. – Vol. 2. – № 1. – P. 53-68.
8. Jogy I. Characteristics and classification of plasmas // PlasTEP trainings course and Sammer school. – 2011. – P. 1-33
9. Bunu M.S., Sasikala P., Dhanapal A., Kavitha V., Yazhini G., Rajamani L. Cold plasma as a novel food processing technology // International Jornal of Emerging trends in Engineering and Development. – 2012. – Vol. 4. – № 2. – P. 803-818.
10. Alejnik A.N., Bajkov A.N., Dambaev G.C., Deneko O.I., Zhdanova O.S., Krasnozhenov E.P., Semichev E.V. Osobennosti vzaimodejstvija neravnovesnoj plazmy s zhivymi tkanjami // Vestnik nauki Sibiri. – 2012. – Vol. 3. – № 4. – S. 44-48.
11. Fridman G., Peddinghaus M., Ayan H., Fridman A., Balasubramanian M., et al. Blood Coagulation and Living Tissue Sterilization by Floating-Electrode Dielectric Barrier Discharge in Air // Plasma Chemistry and Plasma Processing. – 2006. – Vol.26. – P. 425–442.
12. Fridman G., Shereshevsky A., Jost M.M., Brooks A.D., Fridman A., Gutsol A., et al. Floating Electrode Dielectric Barrier Discharge Plasma in Air Promoting Apoptotic Behavior in Melanoma Skin Cancer Cell Lines // Plasma Chemistry and Plasma Processing. – 2007. – Vol. 27. – № 2. – P. 163–176.
13. Panngom K., Baik K.Y., Nam M.K., Han J.H., Rhim H., Choi E.H. Preferential killing of human lung cancer cell lines with mitochondrial dysfunction by nonthermal dielectric barrier discharge plasma // Cell Death Dis. – 2013. – Vol. 4. – № 5. – e642.
14. Panngom K., Lee S.H., Park D.H., Sim G.B., Kim Y.H., Uhm H.S. Non-Thermal Plasma Treatment Diminishes Fungal Viability and Up-Regulates Resistance Genes in a Plant Host // PLoS One. – 2014. – Vol. 9. – № 6. – e99300.
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Zhunussova, A. S., Orynbayeva, Z. S., & Tuleukhanov, S. T. (2016). The effects of low temperature atmospheric plasma on viability of metastatic prostate cancer cells. Experimental Biology, 64(2), 86–93. Retrieved from https://bb.kaznu.kz/index.php/biology/article/view/1067
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BIOPHYSICS
