Ірі мүйізді малдың микросателлиті локустар бірдейлестіру үшін модификацияланған олигонуклеотидтердің синтезі. Synthesis of the modified primers for identification of microsattelite loci of a horned cattle
Ключевые слова:
олигонуклеотидтер, флуоресцентік белгі, синтез, микросателлиттер. oligonucleotides, fluorescent labels, synthesis, microsatellitesАннотация
Қазiргi таңда, түрлердi жəне популяцияларды геномды картiлеу жəне туыстығын анықтау барысында популяциялық жəне эволюциялық биологияда флуоресценттi-модификацияланған олигонуклеотидтер негiзiнде микросателлиттi ДНК технологиялары өте информативтi жəне тиімді. Жұмыс жүргізу барысында біз BМ1818, BM 1824, BM2113 жəне CSRM60 праймерлерін 5' соңынан модифицирлендi. Азидтi флуоресценттi родаминдермен қосылган праймерлер бос олигонуклеотидтерден жəне қосылмаган флуоресценттi реагентпен C18 колонкасында ЖТСХ жүйесiнде тазаланды. Хроматография фракциясы арқылы алынған праймерлер фракциясы флуоресценттi родамин эмиссиясының толкын ұзындығы арқылы анализдендi Today microsatellite DNA technologies, which based of fluorescent-modified oligonucleotides, are be most informing and effective applying in population and evolutionary biology, genomic mapping and relationship between types and populations. We modified 4 primers for BM1818, BM1824, BM2113 и CSRM60 loci. Primers, which connected with azids of fluorescent rhodamines, were cleared from free primers and not bound fluorescent reagent on C18 column in HPLC system. Fractions of primers, which received by means of a HPLC, were analyzed on length of a wave of emission of fluorescent rhodamine.Библиографические ссылки
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the study of an associated quantitative trait locus // PNAS. - 1993. -V. 90. - P.1058 - 1062.
2. Band M, Ron M. Isolation of polymorphic AGC repeats located 3' to bovine SINEs // Animal Genetics. - 1994. -V. 25. -
P.281-283
3. Charlier С., Coppieters W., Farnir F. et al. The mh gene causing double-muscling in cattle maps to bovine Chromosome 2
// Mamm. Genome. - 1995.- V.6. - P. 788-792
4. Georges M.D., Nielsen M., Mackinnnon M. et al. Mapping quantitative trait loci controlling milk production in dairy
cattle by exploiting progeny testing // Genetics. - 1995. - V.139. - P. 907-920.
5. Ohba Y., Kitagawa H., Kunieda Т., et al. Mapping of a locus responsible for a new hereditary disease for renal tubular
dysplasia in Japanese Black cattle // Anim. Genet. - 1998. - V. 29. - P. 65
6. Velmala R.J., Vilkki J.H., Elo K.T. A search for quantitative trait loci for milk production traits on chromosome 6 in
Finnish Ayrshire cattle. // Animal Genetics. - 1999. - V.30. -P.136-143.
7. Ashwell M.S., Van Tassell C.D. Detection on putative loci atfecting milk, health and type traits is a US Holstein
population using 70 microsatellite markers in agenome scan. // J. Dairy Sc. - 1999. - V.82. - №.11. -P.2497-2502
8. Ma R, I Russ Z., Park C., Heyen D.W., Beever J.E., Green C.A., Lewin H.A. Isolation and characterization of 45
polymorphic microsatellites from the bovine genome. // Animal Genetics. - 1996. - V.27. - P.43-47.
9. Kato Y.,Tani Т., Sotomaru Y., Kurokawa K., Kato J. Eight calves cloned from somatic cells of a singfe adult. // Science. -
1998. - V.282. -P.2095-2098.
10. Peelman L., Mortiaux F., Van Zeveren A., Dansercoer A. Evaluation to the genetic variability of 23 bovine microsatellite
markers in tour Belgian cattle breeds. // Animal Genetics. - 1998. - V.29. - V.3. - P. 161167.
11. Vemala R., Vilkki J., Elo K. Gasein haplotypes and their association with milk production traits in the Finnish Ayrshire
cattle. // Animal Genetics. - 1995. - V.26. - P.419-426.
12. Astakhova, K.; Wengel, J. Interfacing Click Chemistry with Automated Oligonucleotide Synthesis for the Preparation of
Fluorescent DNA Probes Containing Internal Xanthene and Cyanine Dyes // Chem. Eur. J. – 2013.- V.19(3). –P.1112–1122.
13. Ellington A, Pollard JD Jr. Purification of oligonucleotides using denaturing polyacrylamide gel electrophoresis // Curr
Protoc Mol Biol. – 2001. – V.1
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БИОТЕХНОЛОГИЯ: ОТ ИССЛЕДОВАНИЙ К ИННОВАЦИЯМ
