Шар нуур көлінен токсин түзуші цианобактериялардың жаңа дақылдарын бөліп алу және идентификациялау
120 44
Кілттік сөздер:
цианобактерия, бактериологиялық таза дақыл, морфология, токсиндар, идентификация, штамм Desertifilum sp.1Аннотация
Мақалада Моңғолияның Баян Өлгей аймағында орналасқан Шар Нуур (Сары көл) көлінің альгофлора құрамы зерттелді. Зерттелген су үлгілерінен Oscillatoriaceae және Nostocaceae тұқымдасының 3 бактериологиялық таза дақыл бөлініп алынды. Н.С. Строганов жүйесі бойынша бөлініп алынған дақылдардың токсинділігі анықталды. Тест-объект дафнияға қатынасы бойынша дақыл SP-O1 өте улы болды. Токсин түзуші дақылдың ісік жасуша HeLa тест-объектісіне қатынасын бағалау бойынша цитотоксинді белсенділік көрсетті. SP-O1 штамының экстрактісін зерттеу нәтижесі бойынша қауіпті токсиндер анықталған жоқ. Идентификацияланған токсиндер негізінен микроцистиндерге қарайды. Шар Нуур көлінен бөлініп алынған цианобактерия SP-O1 дақылы ботаникалық белгілері бойынша Oscillatoria туысына жатқызылғанмен, генетикалық сараптама бойынша олар Oscillatoriaceae тұқымдасының Desertifilum туысына жоғары гомологияны көрсетті. Осы мәліметтерге негізделе отырып SP-O1 дақылы идентификацияланып Desertifilum sp.1. деген атау берілді.
Библиографиялық сілтемелер
1. Carmichael W.W. The toxins of Cyanobacteria // Sci. Amer. - 1994. - №1. - P. 78–86.
2. Ballot A, Fastner J, and Wiedner C. Paralytic shellfish poisoning toxin-producing cyanobacterium Aphanizomenon gracile in northeast Germany // Appl. Environ. Microbiol. - 2010. - V. 76. - P. 1173–1180.
3. Namikoshi M., Rinehart K.L. Bioactive compounds produced by cyanobacteria // J. Industr. Microbiol. Biotechn.- 1996. - V. 17. - P. 373–384.
4. Harada K.I. Production of secondary metabolites by freshwater cyanobacteria // Chem. Pharm. Bull. - 2004. - V. 5. - P. 889–899.
5. Определитель сине-зеленых водорослей СССР // Отв. ред. Голлербах М.М. Л.: Наука. - 1951. - С. 1–14.
6. Andersen R.A. Algal Culturing Techniques // New York, NY, U.S.A. Elsevier Academic Press. - 2005. – Р. 578.
7. Темралеева А.Д., Минчева Е.В., Букин Ю.С., Андреева А.М. Современные методы выделения, культивирования и идентификации зеленых водорослей (Chlorophyta) // Кострома: Костромской печатный дом. - 2014. - 215 с.
8. Jones A.K., Muriel E., Rhodes M.E., Evans S.C. The use of antibiotics to obtain axenic cultures of algae // Brit. Phycol. J. - 1973. - V. 8, №1. - P. 185–196.
9. Day Chronictoxicity test using Daphnia magna or Daphnia pulex // - 1994. SOP №2028:https://clu-in.org/download/ert/2028-R00.pdf.
10. Волошко Л.Н., Плющ А.В., Титова Н.Н. Токсины Цианобактерий (Cyanobacteia, Cyanophyta)// Альгология.-2008.-Т.18, №1.-С.3-21.
11. Brittain S., Mohamed Z.A., Wang J., Lehmann V.K.B. Isolation and characterization of microcystins from a river Nile strain of Oscillatoria tenuis Agardh ex Gomont // Toxicon. - 2000. - V. 38, № 12. - P. 1759–1771.
12. Freshney R. I. Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications, Sixth Edition ISBN: 978-0-470-52812-9. – 2001. - 796 p.
13. Dittman E., Fewer D.P., Neilan B.A. Cyanobacterial toxins: biosynthetic routes and evolutionary routes // FEMS Microbiol. Rev. - 2013. - V.37. - P. 23–43.
14. Bell S.G., Codd G.A. Cyanobacterial toxins and human health // Rev. Med. Microbiol. - 1994.- № 4. - P. 256-264.
15. Kardinal W.E.A., Visser P.M. Dynamics of cyanobacteria toxins. Sources of variability in
microcystin concentratio ns // Harmful cyanobacteria. Netherlands: Spinger, - 2005. - P. 41 -63.
16. Al-Sultan E.Y.A. The Isolation, the purification and the identification of hepatotoxin Microcystin-LR from two cyanobacterial species and studying biological activity on some aquatic organisms // J. Basrah Res. (Sci.). - 2011. - V. 37. - P. 39–57.
17. Codd G.A. Cyanobacterial toxins: occurrence, properties and biological significance // Wat. Sci. Tech. - 1995. - V. 32. - P.149–156.
18. Chaganty S., Golakoti T., Heltzel C., Moore R.E., Yoshida W.Y. Isolation and structure determination of cryptophycins 38, 326, and 327 from the terrestrial cyanobacterium Nostoc sp. GSV 224 // J. Nat. Prod. - 2004. - V. 67. - P.1403–1406.
19. Trimurtulu G., Ogino J., Helsel C.E., Husebo, Jensen C.M., Larsen L.K., Patterson G.M.L., Moore R.E., Mooberry S.I., Corbett T.H., Valeriote F.A. Structure determination, conformational analysis, chemical stability studies, and antitumor evaluation of the cryptophycins. Isolation of 18 new analogs from Nostoc sp. strain GSV 224 // J. Amer. Chem. Soc. - 1995. - V. 117. - P. 12030–12049.
20. Okino T., Murakami M., Haraguchi R., Munekata H., Matsuda H., Yamaguchi K. Micropeptins A and B, plasmin and trypsin inhibitors from the blue-green alga Microcystis aeruginosa // Tetrahedron Lett. - 1993. - V. 34, № 50. - P. 8131–8134.
References
1. Carmichael W.W. (1994) The toxins of Cyanobacteria, Sci. Amer. 1: 78–86.
2. Ballot A, Fastner J, and Wiedner C. (2010) Paralytic shellfish poisoning toxin-producing cyanobacterium Aphanizomenon gracile in northeast Germany, Appl. Environ. Microbiol. 76:1173–1180.
3. Namikoshi M., Rinehart K.L. (1996) Bioactive compounds produced by cyanobacteria, J. Industr. Microbiol. Biotechn. 17: 373–384.
4. Harada K.I. (2004) Production of secondary metabolites by freshwater cyanobacteria, Chem. Pharm. Bull. 5:889–899.
5. Gollerbah M.M.. (1951) The determinant of blue-green algae of the USSR, L: Nauka, pp. 1-14.
6. Andersen R.A. (2005) Algal Culturing Techniques, New York, NY, U.S.A. Elsevier Academic Press. pp. 578.
7. Tamraleeva A.D., Mincheva E.V., Bukin U.S., Andreeva A.M. (2014) Modern methods of isolation, cultivation and identification of green algae (Chlorophyta). Kostroma. [Sovremennye metody vydeleniya, kultivirovaniya I identifikacia zelenyh vodoroslei (Chlorophyta). – Kostroma] :215. (In Russian).
8. Jones A.K., Muriel E., Rhodes M.E., Evans S.C. (1973) The use of antibiotics to obtain axenic cultures of algae, Brit. Phycol. J. 8(1-2):185–196.
9. Day (1994) Chronictoxicity test using Daphnia magna or Daphnia pulex, SOP, 2028:https://clu-in.org/download/ert/2028-R00.pdf.
10. Voloshko L.N, Plush A.V., Titova N.N. (2008) Toxins Cyanobacteria. Algology (Cyanobacteia, Cyanophyta) [Toksiny Cianobakterii (Cyanobacteia, Cyanophyta). Algologia] 18 (1):3-21. (In Russian)
11. Brittain S., Mohamed Z.A., Wang J., Lehmann V.K.B (2000) Isolation and characterization of microcystins from a river Nile strain of Oscillatoria tenuis Agardh ex Gomont, Toxicon. 38 (12): 1759–1771.
12. Freshney R.I. (2005) Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications, Sixth Edition ISBN: 978-0-470-52812-9. P. 796.
13. Dittman E., Fewer D.P. (2013) Neilan B.A. Cyanobacterial toxins: biosynthetic routes and evolutionary routes, FEMS Microbiol. Rev.37: 23–43.
14. Bell S.G., Codd G.A. (1994) Cyanobacterial toxins and human health, Rev. Med. Microbiol. 4: 256-264.
15. Kardinal W.E.A., Visser P.M. (2005) Dynamics of cyanobacteria toxins. Sources of variability in microcystin concentratio ns, Harmful cyanobacteria. Netherlands: Spinger, 41 -63.
16. Al-Sultan E.Y.A. (2011) The Isolation, the purification and the identification of hepatotoxin Microcystin-LR from two cyanobacterial species and studying biological activity on some aquatic organisms, J. Basrah Res. (Sci.). 37:39–57.
17. Codd G.A. (1995) Cyanobacterial toxins: occurrence, properties and biological significance, Wat. Sci. Tech. 32:149–156.
18. Chaganty S., Golakoti T., Heltzel C., Moore R.E., Yoshida W.Y. (2004) Isolation and structure determination of cryptophycins 38, 326, and 327 from the terrestrial cyanobacterium Nostoc sp. GSV 224, J. Nat. Prod. 67:1403–1406.
19. Trimurtulu G., Ogino J., Helsel C.E., Husebo, Jensen C.M., Larsen L.K., Patterson G.M.L., Moore R.E., Mooberry S.I., Corbett T.H., Valeriote F.A. (1995) Structure determination, conformational analysis, chemical stability studies, and antitumor evaluation of the cryptophycins. Isolation of 18 new analogs from Nostoc sp. strain GSV 224, J. Amer. Chem. Soc. 117:12030–12049.
20. Okino T., Murakami M., Haraguchi R., Munekata H., Matsuda H., Yamaguchi K. (1993) Micropeptins A and B, plasmin and trypsin inhibitors from the blue-green alga Microcystis aeruginosa. Tetrahedron Lett. 34(50):8131–8134.
2. Ballot A, Fastner J, and Wiedner C. Paralytic shellfish poisoning toxin-producing cyanobacterium Aphanizomenon gracile in northeast Germany // Appl. Environ. Microbiol. - 2010. - V. 76. - P. 1173–1180.
3. Namikoshi M., Rinehart K.L. Bioactive compounds produced by cyanobacteria // J. Industr. Microbiol. Biotechn.- 1996. - V. 17. - P. 373–384.
4. Harada K.I. Production of secondary metabolites by freshwater cyanobacteria // Chem. Pharm. Bull. - 2004. - V. 5. - P. 889–899.
5. Определитель сине-зеленых водорослей СССР // Отв. ред. Голлербах М.М. Л.: Наука. - 1951. - С. 1–14.
6. Andersen R.A. Algal Culturing Techniques // New York, NY, U.S.A. Elsevier Academic Press. - 2005. – Р. 578.
7. Темралеева А.Д., Минчева Е.В., Букин Ю.С., Андреева А.М. Современные методы выделения, культивирования и идентификации зеленых водорослей (Chlorophyta) // Кострома: Костромской печатный дом. - 2014. - 215 с.
8. Jones A.K., Muriel E., Rhodes M.E., Evans S.C. The use of antibiotics to obtain axenic cultures of algae // Brit. Phycol. J. - 1973. - V. 8, №1. - P. 185–196.
9. Day Chronictoxicity test using Daphnia magna or Daphnia pulex // - 1994. SOP №2028:https://clu-in.org/download/ert/2028-R00.pdf.
10. Волошко Л.Н., Плющ А.В., Титова Н.Н. Токсины Цианобактерий (Cyanobacteia, Cyanophyta)// Альгология.-2008.-Т.18, №1.-С.3-21.
11. Brittain S., Mohamed Z.A., Wang J., Lehmann V.K.B. Isolation and characterization of microcystins from a river Nile strain of Oscillatoria tenuis Agardh ex Gomont // Toxicon. - 2000. - V. 38, № 12. - P. 1759–1771.
12. Freshney R. I. Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications, Sixth Edition ISBN: 978-0-470-52812-9. – 2001. - 796 p.
13. Dittman E., Fewer D.P., Neilan B.A. Cyanobacterial toxins: biosynthetic routes and evolutionary routes // FEMS Microbiol. Rev. - 2013. - V.37. - P. 23–43.
14. Bell S.G., Codd G.A. Cyanobacterial toxins and human health // Rev. Med. Microbiol. - 1994.- № 4. - P. 256-264.
15. Kardinal W.E.A., Visser P.M. Dynamics of cyanobacteria toxins. Sources of variability in
microcystin concentratio ns // Harmful cyanobacteria. Netherlands: Spinger, - 2005. - P. 41 -63.
16. Al-Sultan E.Y.A. The Isolation, the purification and the identification of hepatotoxin Microcystin-LR from two cyanobacterial species and studying biological activity on some aquatic organisms // J. Basrah Res. (Sci.). - 2011. - V. 37. - P. 39–57.
17. Codd G.A. Cyanobacterial toxins: occurrence, properties and biological significance // Wat. Sci. Tech. - 1995. - V. 32. - P.149–156.
18. Chaganty S., Golakoti T., Heltzel C., Moore R.E., Yoshida W.Y. Isolation and structure determination of cryptophycins 38, 326, and 327 from the terrestrial cyanobacterium Nostoc sp. GSV 224 // J. Nat. Prod. - 2004. - V. 67. - P.1403–1406.
19. Trimurtulu G., Ogino J., Helsel C.E., Husebo, Jensen C.M., Larsen L.K., Patterson G.M.L., Moore R.E., Mooberry S.I., Corbett T.H., Valeriote F.A. Structure determination, conformational analysis, chemical stability studies, and antitumor evaluation of the cryptophycins. Isolation of 18 new analogs from Nostoc sp. strain GSV 224 // J. Amer. Chem. Soc. - 1995. - V. 117. - P. 12030–12049.
20. Okino T., Murakami M., Haraguchi R., Munekata H., Matsuda H., Yamaguchi K. Micropeptins A and B, plasmin and trypsin inhibitors from the blue-green alga Microcystis aeruginosa // Tetrahedron Lett. - 1993. - V. 34, № 50. - P. 8131–8134.
References
1. Carmichael W.W. (1994) The toxins of Cyanobacteria, Sci. Amer. 1: 78–86.
2. Ballot A, Fastner J, and Wiedner C. (2010) Paralytic shellfish poisoning toxin-producing cyanobacterium Aphanizomenon gracile in northeast Germany, Appl. Environ. Microbiol. 76:1173–1180.
3. Namikoshi M., Rinehart K.L. (1996) Bioactive compounds produced by cyanobacteria, J. Industr. Microbiol. Biotechn. 17: 373–384.
4. Harada K.I. (2004) Production of secondary metabolites by freshwater cyanobacteria, Chem. Pharm. Bull. 5:889–899.
5. Gollerbah M.M.. (1951) The determinant of blue-green algae of the USSR, L: Nauka, pp. 1-14.
6. Andersen R.A. (2005) Algal Culturing Techniques, New York, NY, U.S.A. Elsevier Academic Press. pp. 578.
7. Tamraleeva A.D., Mincheva E.V., Bukin U.S., Andreeva A.M. (2014) Modern methods of isolation, cultivation and identification of green algae (Chlorophyta). Kostroma. [Sovremennye metody vydeleniya, kultivirovaniya I identifikacia zelenyh vodoroslei (Chlorophyta). – Kostroma] :215. (In Russian).
8. Jones A.K., Muriel E., Rhodes M.E., Evans S.C. (1973) The use of antibiotics to obtain axenic cultures of algae, Brit. Phycol. J. 8(1-2):185–196.
9. Day (1994) Chronictoxicity test using Daphnia magna or Daphnia pulex, SOP, 2028:https://clu-in.org/download/ert/2028-R00.pdf.
10. Voloshko L.N, Plush A.V., Titova N.N. (2008) Toxins Cyanobacteria. Algology (Cyanobacteia, Cyanophyta) [Toksiny Cianobakterii (Cyanobacteia, Cyanophyta). Algologia] 18 (1):3-21. (In Russian)
11. Brittain S., Mohamed Z.A., Wang J., Lehmann V.K.B (2000) Isolation and characterization of microcystins from a river Nile strain of Oscillatoria tenuis Agardh ex Gomont, Toxicon. 38 (12): 1759–1771.
12. Freshney R.I. (2005) Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications, Sixth Edition ISBN: 978-0-470-52812-9. P. 796.
13. Dittman E., Fewer D.P. (2013) Neilan B.A. Cyanobacterial toxins: biosynthetic routes and evolutionary routes, FEMS Microbiol. Rev.37: 23–43.
14. Bell S.G., Codd G.A. (1994) Cyanobacterial toxins and human health, Rev. Med. Microbiol. 4: 256-264.
15. Kardinal W.E.A., Visser P.M. (2005) Dynamics of cyanobacteria toxins. Sources of variability in microcystin concentratio ns, Harmful cyanobacteria. Netherlands: Spinger, 41 -63.
16. Al-Sultan E.Y.A. (2011) The Isolation, the purification and the identification of hepatotoxin Microcystin-LR from two cyanobacterial species and studying biological activity on some aquatic organisms, J. Basrah Res. (Sci.). 37:39–57.
17. Codd G.A. (1995) Cyanobacterial toxins: occurrence, properties and biological significance, Wat. Sci. Tech. 32:149–156.
18. Chaganty S., Golakoti T., Heltzel C., Moore R.E., Yoshida W.Y. (2004) Isolation and structure determination of cryptophycins 38, 326, and 327 from the terrestrial cyanobacterium Nostoc sp. GSV 224, J. Nat. Prod. 67:1403–1406.
19. Trimurtulu G., Ogino J., Helsel C.E., Husebo, Jensen C.M., Larsen L.K., Patterson G.M.L., Moore R.E., Mooberry S.I., Corbett T.H., Valeriote F.A. (1995) Structure determination, conformational analysis, chemical stability studies, and antitumor evaluation of the cryptophycins. Isolation of 18 new analogs from Nostoc sp. strain GSV 224, J. Amer. Chem. Soc. 117:12030–12049.
20. Okino T., Murakami M., Haraguchi R., Munekata H., Matsuda H., Yamaguchi K. (1993) Micropeptins A and B, plasmin and trypsin inhibitors from the blue-green alga Microcystis aeruginosa. Tetrahedron Lett. 34(50):8131–8134.
Жүктелулер
Как цитировать
Bolatkhan, K., Kopecky, J., Zhambakin, K. Z., Los, D. A., Sinetova, M. A., Akmukhanova, N. R., Sadvakasova, A. K., & Zayadan, B. K. (2018). Шар нуур көлінен токсин түзуші цианобактериялардың жаңа дақылдарын бөліп алу және идентификациялау. ҚазҰУ Хабаршысы. Биология сериясы, 70(1), 110–119. вилучено із https://bb.kaznu.kz/index.php/biology/article/view/1257
Шығарылым
Бөлім
МИКРОБИОЛОГИЯ
