Translation in vitro of synthetic HvNHX3, AtDREB1A and AtDREB2A mRNA.
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Keywords:
Transcription, translation, трансляция, транскрипция, HvNHX3, AtDREB1A, AtDREB2A,Abstract
The task of this work is optimization of in vitro translation process of HvNHX3, AtDREB1A, AtDREB2A mRNAs in cell free wheat system and further its diagnostic using [32S]-methionine. Protein products were obtained as the result all kind mRNAs synthesis after SDS-PAAG electrophoresis. Also we determined optimal conditions for product diagnostic of mRNA HvNHX3 translation using different detergents.References
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ARC-1, a sequence element complementary to an internal 18S rRNA segment, enhances translation efficiency in plants
when present in the leader or intercistronic region of mRNAs // Nucleic Acids Research. - 2004. - V. 32. - No.1.–P.239-247.
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2 Bassil T. Antiporters NHX1 and NHX2 Control Vacuolar pH and K+ Homeostasis to Regulate Growth, Flower Development, and Reproduction The Arabidopsis Na+/H+ // The Plant Cell. – 2011. - Vol. 23. – Р. 3482-3497.
3 Liu Q., Kasuga M., Sakuma Y., Abe H., Miura S., Yamaguchi-Shinozaki K., Shinozaki K. Two transcription factors, DREB1 and DREB2, with an EREBP/AP2 DNA binding domain separate two cellular signal transduction pathways in drought-and low temperature-responsive gene expression, respectively, in Arabidopsis // Plant Cell. – 1998. -Vol.10. – Р. 1391–1406
4 Abdelaty S. Plant AP2/ERF transcription factors //Genetika. – 2003. - Vol. 35, №1. – P. 37-50.
5 Akbergenov R.Zh., Zhanybekova S.Sh., Kryldakov R.V., Zhigailov A.V., Polimbetova N.S., Hohn T., Iskakov B.K.
ARC-1, a sequence element complementary to an internal 18S rRNA segment, enhances translation efficiency in plants
when present in the leader or intercistronic region of mRNAs // Nucleic Acids Research. - 2004. - V. 32. - No.1.–P.239-247.
6 Sakuma Y. Functional analysis of an Arabidopsis transcription factor, DREB2A, involved in drought-responsive gene expression // Plant Cell. – 2006. - №18. – P. 1292–1309.
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Nargilova, R. M., Karpova, O. V., Pissarenko, A. M., & Iskakov, B. K. (2015). Translation in vitro of synthetic HvNHX3, AtDREB1A and AtDREB2A mRNA. Experimental Biology, 60(2), 294–297. Retrieved from https://bb.kaznu.kz/index.php/biology/article/view/956
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