MESENCHYMAL STEM CELL IN HYALURONIC ACID SCAFFOLD AS A THERAPEUTIC TOOL FOR OSTEOARTHRITIS – PRELIMINARY REPORT.
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Keywords:
Osteoarthritis, stem cells (MSC),Abstract
Mesenchymal stem cells (MSC) are currently in the focus of attention worldwide as a potential therapeutic tool for osteoarthritis and other types of cartilage damage. Today, a broad range of MSC sources and scaffolds are being tested worldwide to develop a novel cellular therapeutic product. To explore the efficiency of MSC derived from synovial membrane in hyaluronic acid (HA), a rabbit model of osteoarthritis was created by introducing a full-thickness defect onto a cartilage of the medial condyle of rabbit hind limb. Our preliminary results indicate a dramatic improvement in cartilage regeneration under MSC therapy. Also, aiming to optimize culture conditions for MSC, we found optimal concentration of HA to be used for in vitro cell propagation and transplantation.References
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2. The burden of musculoskeletal conditions at the start of the new millennium. World Health Organ Tech Rep Ser 2003, 919:i-x, 1-218, back cover.
3. March LM, Cross M, Tribe KL, Lapsley HM, Courtenay BG, Cross MJ, Brooks PM, Cass C, Coolican M, Neil M, Pinczewski L,Quain S, Robertson F, Ruff S, Walter W, Zicat B: Two knees or not two knees? Patient costs and outcomes following bilateral and unilateral total knee joint replacement surgery for OA. Osteoarthritis Cartilage 2004, 12:400-408.
4. Grigolo B, Lisignoli G, Desando G, Cavallo C, Marconi E, Tschon M, Giavaresi G, Fini M, Giardino R, Facchini A: Osteoarthritis treated with mesenchymal stem cells on hyaluronan-based scaffold in rabbit. Tissue Eng Part C Methods 2009, 15:647-658.
5. Prockop DJ: Marrow stromal cells as stem cells for nonhematopoietic tissues. Science 1997, 276:71-74.
6. Csaki C, Schneider PR, Shakibaei M: Mesenchymal stem cells as a potential pool for cartilage tissue engineering. Ann Anat 2008, 190:395-412.
7. Noth U, Steinert A, Tuan R: Technology insight: adult mesenchymal stem cells for osteoarthritis therapy. Nat Clin Pract Rheumatol 2008, 4:371-380.
8. Murphy J, Fink D, Hunziker E, Barry F: Stem cell therapy in a caprine model of osteoarthritis. Arthritis Rheum 2003, 48:3464-3474.
9. Wakitani S, Imoto K, Yamamoto T, Saito M, Murata N, Yoneda M: Human autologous culture expanded bone marrow mesenchymal cell transplantation for repair of cartilage defects in osteoarthritic knees. Osteoarthritis Cartilage 2002, 10:199-206.
10. Kayakabe M, Tsutsumi S, Watanabe H, Kato Y, Takagishi K: Transplantation of autologous rabbit BM-derived mesenchymal stromal cells embedded in hyaluronic acid gel sponge into osteochondral defects of the knee. Cytotherapy 2006, 8:343-353.
11. Liu Y, Shu X, Prestwich G: Osteochondral defect repair with autologous bone marrow-derived mesenchymal stem cells in an injectable, in situ, cross-linked synthetic extracellular matrix. Tissue Eng 2006, 12:3405-3416.
12. Campoccia D, Doherty P, Radice M, Brun P, Abatangelo G, Williams DF: Semisynthetic resorbable materials from hyaluronan esterification. Biomaterials 1998, 19:2101-2127.
13. Solchaga L, Temenoff J, Gao J, Mikos A, Caplan A, Goldberg V: Repair of osteochondral defects with hyaluronan- and polyesterbased
scaffolds. Osteoarthritis Cartilage 2005, 13:297-309.
14. Loken S, Jakobsen RB, Aroen A, Heir S, Shahdadfar A, Brinchmann JE, Engebretsen L, Reinholt FP: Bone marrow mesenchymal stem cells in a hyaluronan scaffold for treatment of an osteochondral defect in a rabbit model. Knee Surg Sports Traumatol Arthrosc 2008,16:896-903.
15. Baier L, Bivens K, Patrick C, Schmidt C: Photocrosslinked hyaluronic acid hydrogels: natural, biodegradable tissue engineering scaffolds. Biotechnol Bioeng 2003, 82:578-589.
16. De B, Dell'Accio F, Tylzanowski P, Luyten FP: Multipotent mesenchymal stem cells from adult human synovial membrane. Arthritis Rheum 2001, 44:1928-1942.
17. Lai W, Krishnappa V, Phinney D: Fibroblast growth factor 2 (Fgf2) inhibits differentiation of mesenchymal stem cells by inducing Twist2 and Spry4, blocking extracellular regulated kinase activation, and altering Fgf receptor expression levels. Stem Cells 2011, 29:1102-1111.
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Bekbolsynov, D. A., Mukhambetova, A. E., Ogay, V. B., & Raimagambetov, E. K. (2017). MESENCHYMAL STEM CELL IN HYALURONIC ACID SCAFFOLD AS A THERAPEUTIC TOOL FOR OSTEOARTHRITIS – PRELIMINARY REPORT. Experimental Biology, 56(4), 355–358. Retrieved from https://bb.kaznu.kz/index.php/biology/article/view/937
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INNOVATIONAL BIOLOGY and BIOTECHNOLOGY
