Producing root cultures of the Hedysarum enaffae
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Keywords:
endemic, Hedysarum, medicinal plants, isolated roots, “hairy roots”, root culture in vitro, Agrobacterium rhizogenes, biologically active substances.Abstract
Root cultures produced using wild non-modified Agrobacterium strains are of high practical interest in terms of their biological safety for development fitodrugs on the base of medicinal plants. Root cultures of Hedysarum enaffae were produced by inoculation with Agrobacterium rhizogenes 15834Sw strain and their morphological characteristics were studied. Isolated roots were characterized with dark coloring and both root types had capacity to stem organogenesis. Hairy roots had light coloring and were a mixture of clear appeared roots with small quantity of proliferating suspension cells which are separated from friable surface of growing roots and grow in suspension form. Both root types had high growing rate and root indexes after cultivation during 8 weeks were 30 at isolated and 50 at hairy roots accordingly.References
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147-194.
2 Giri A. Transgenic hairy roots: Recent trends and applications [Text] / A. Giri, M.L. Narasu // Biotechnology Advances. 2000.
– V. 18. – P. 1-22.
3 Кузовкина, И.Н. Генетически трансформированные корни растений как потенциальный источник экологически чистого
лекарственного сырья [Текст] / И.Н. Кузовкина, И.Е. Альтерман, М.Ю. Вдовитченко // 2-й Всероссийский симпозиум “Физио-
логия трансгенного растения и проблемы биобезопасности”: Программа и тезисы докладов, Институт физиологии растений
им. К.А. Тимирязева РАН. – М., 2007. – С. 54.
4 Aird, E.H. Cytogenetic Analysis of Hairy Root Cultures from a Number of Plant Species Transformed with Agrobacterium rhizogenes
[Text] / E.H. Aird, J.D. Hamill, M.J.C. Rhodes // Plant Cell, Tissue Organ Cult. – 1988. – V. 15. – P. 47-57.
5 Myers, N. Biodiversity hotspots for conservation priorities. [Text]/ N. Myers, R. A. Mittermeier, C. G. Mittermeier, G. A B. da
Fonseca, J. Kent. // Nature. – 2000. – V. 403. – P. 853–858.
6 Chen, S.G. Prenylisoflavone derivatives from the roots of Hedysarum scoparium [Text] / S.G. Chen, J.J. Chen, K. Gao // Chem.
Pharm. Bull. (Tokyo). – 2007. – V.55. – N 8. – P. 1181-1184.
7 Пеккер, Е.Т. К биохимической характеристике растений рода Hedysarum L. Юго-Восточного Алтая [Текст] / Е.Т. Пеккер
// Актуальные вопросы ботанического ресурсоведения в Сибири. – Новосибирск, 1976. – С.146-149.
8 Mugnier, J. Establishment of new axenic hairy root lines by inoculation with Agrobacterium rhizogenes. [Text] / J. Mugnier //
Plant Cell Reports. – 1988. – V. 7. – P. 9-12.
9 Tepfer, D. Genetic transformation using Agrobacterium rhizigenes. [Text] / D. Tepfer //Physiologia Plantarum. – 1990. – V. 79.
– P. 140-146. Technical Information Sheet #4, 2008.
10 Gamborg, O.L.
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Umralina, A. R. (2015). Producing root cultures of the Hedysarum enaffae. Experimental Biology, 58(2), 61–65. Retrieved from https://bb.kaznu.kz/index.php/biology/article/view/466
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BIOTECHNOLOGY
