The influence of Z-ligustilid on the generation of superoxide anion and the phosphorylation of cPLA2 in astrocytes at β-amyloid
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Keywords:
β-amyloid, Z-ligustilid, rats astrocytes, reactive oxygen species, superoxide dismutase,Abstract
В настоящей работе было изучено влияние Z-лигустилида (бутилиден-4,5-дигидрофталид, на генерацию супероксид аниона и фосфорилирование cPLA2 в астроцитах крыс при воздействии β-Амилоида. Установлено, что Z-лигустилид ингибирует генерацию супероксид аниона, синтез которого индуцирует β-Амилоид. Показано, что Z-лигустилид снижает уровень фосфорилирования cPLA2, но при этом не оказывает токсического действия на астроциты.References
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Chem. Soc. - 2005. -127. – P. 2075-2084.
3 Abramov A.Y., Duchen M.R. The role of an astrocytic NADPH oxidase in the neurotoxicity of amyloid beta peptides // Biol Sci. – 2005. -V. 360. – P. 2309-2314.
4 Park L., Zhou P., Pitstick R., Capone C., Anrather J., Norris E.H., Younkin L., Younkin S., Carlson G., McEwen B.S., Iadecola C. Nox2-derived radicals contribute to neurovascular and behavioral dysfunction
in mice overexpressing the amyloid precursor protein // Proc. Nat. Ac. Sci. - 2008. - V. 105. - P. 1347-1352.
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6 Stephenson D., Rash K., Smalstig B., Roberts E., Johnstone E., Sharp J., Panetta J., Little S., Kramer R., Clemens J. Cytosolic phospholipase A2 is induced in reactive glia following different forms of neurodegeneration
// Glia. - 1999. - V. 27. - P. 110-128.
7 Sun G.Y., Horrocks L.A., Farooqui A.A. The roles of NADPH oxidase and phospholipases A2 in oxidative and infl ammatory responses in neurodegenerative diseases // J. Neurochem. - 2007. - V. 103. - P. 1-16.
8 Sun G.Y., Xu J., Jensen M.D., Simonyi A.Phospholipase A2 in the central nervous system: implications for neurodegenerative diseases // J. Lipid Res. - 2004. - V. 45. - P. 205-213.
9 Yan Yu, Jun-Rong Du, Chen-Yuan Wang and Zhong-Ming Qian. Protection against hydrogen peroxide-induced injury by Z-ligustilide in PC12 cells // Experimental brain research. - 2008. - V. 184. - P. 307-312.
10 Fa M., Orozco I.J., Francis Y.I., Saeed F., Gong Y., Arancio O. Preparation of oligomeric beta-amyloid 1-42 and induction of synaptic plasticity impairment on hippocampal slices // J. Vis. Exp. - 2010. - V. 41. - P.1884.
11 Zanetti M., d’Uscio L.V., Peterson T.E., Katusic Z.S., O’Brien T. Analysis of Superoxide Anion Production in Tissue // Hypertension. - 2005. - P. 65-72.
12 Bolt A. and Mahoney B. High-effi ciency blotting of proteins of diverse sizes following sodium dodecyl sulfate–polyacrylamide, gel electrophoresis // Analytical Biochemistry. – 1997. – V. 247. – P. 185-192.
13 Schägger H. Tricine–SDS-PAGE // Nature Protocols. -2006. - V. 1. - P. 16-22.
14 Li W., Murai Y., Okada E., Matsui K., Hayashi S., Horie M., Takano Y. Modifi ed and simplifi ed western blotting protocol: use of intermittent microwave irradiation (IMWI) and 5% skim milk to improve binding
specifi city // Pathol. Int. - 2002. - V. 52(3). - P. 234-238.
15 Dennis-Sykes C.A., Miller W.J., McAleer W.J. A quantitative Western Blot method for protein measurement // J. Biol. Stand. - 1985. - V. 13(4). – P. 309-314.
16 Van Kooten T.G., Klein C.L., Kirkpatrick C.J. Western blotting as a method for studying cell-biomaterial interactions: the role of protein collection // J.Biomed. Mater. Res. - 2001. - V. 54(3). - P. 385-389.
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Tsoy, A. K., Shalahmetova, T. M., Askarova, S. Y., & Umbayev, B. A. (2015). The influence of Z-ligustilid on the generation of superoxide anion and the phosphorylation of cPLA2 in astrocytes at β-amyloid. Experimental Biology, 54(2), 56–60. Retrieved from https://bb.kaznu.kz/index.php/biology/article/view/261
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HISTOLOGY, CYTOLOGY, CELL BIOLOGY
