Кратковременное действие сырой нефти, ванадия и никеля на антиоксидантную защитную систему печени крыс. Егеуқұйрықтар бауырының антиоксиданттық қорғау жүйесіне шикі мұнай, ванадий жəне никельдің қысқа мерзімді əсері
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Ключевые слова:
сырая нефть, ванадий, никель, антиоксидантные ферменты, перекисное окисление липидов, крыса, шикі мұнай, антиоксидантты ферменттер, липидтердің асқын тотығуы, егеуқұйрықтар,Аннотация
Перекисное окисление липидов (ПОЛ) и изменения в антиоксидантной системе были определены в печени белых крыс самцов, получавших раздельно и совместно в течение 4-х недель внутрибрюшинно сырую нефть в концентрации 0,5 мл / кг и ванадий и никель в питьевой воде в концентрациях 150 мг в V/л и 180 мг Ni/л . Можно заключить, что раздельное воздействие сырой нефти, никеля и ванадия вызывали сильное повреждение клеток, о чем свидетельствует увеличение уровня ПОЛ в печени и повышение активности внутриклеточных ферментов АЛТ и АСТ в крови. Совместное действие ванадия и сырой нефти проявляет синергизм и усугубляет этот эффект. Стимуляция синтеза GSH, вызванное воздействием никеля, уменьшает индуцированную воздействием сырой нефтью токсичность. Совместное воздействие ванадия, никеля и сырой нефти характеризуется меньшей токсичностью по сравнению с раздельным воздействием металлов и сырой нефти. 4 апта ішінде құрсақішілік концентрациясы 0,5 мл / кг мұнаймен жəне ауыз суда ерітілген концентрациясы 180 мг Ni/л никельмен, 150 мг в V/л ванадиймен бөлек жəне бірге уландырылған ақ егеуқұйрықтардың бауырында липидтердің асқын тотығының (ЛАТ) мөлшері жəне антиоксиданттық жүйедегі өзгерістер анықталды. Бауырдағы ЛАТ мөлшерінің жəне АЛТ жəне АСТ ферменттерінің қандағы белсенділігінің өсуі, мұнай, никель жəне ванадийдің бөлек уландыруы клеткалардың зор зақымдалуына əкелетінін көрсетеді. Ванадий мен мұнайдың бірлескен əсері синергетикалық қасиетке ие болып, токсикалық нəтижені күшейтеді. Никельдің əсерінен GSH синтезінің белсенденуі мұнайдың уыттылығын төмендетеді. Ванадий, никель жəне мұнайдың бірлескен əсерінің уыттылығы осы заттардың бөлек əсерінің уыттылығынан төмен болып шықты.Библиографические ссылки
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14 Landu S., Everitt B.S. A handbook of statistical analyses using SPSS. – London: Chapman and Hall / CRC press LLC, 2004. – P. 337.
15 Raza M., Al-ShabanahO.A., El-Hadiyah T.M., Al-Majed A.A. Effect of prolonged vigabatrin treatment on hematological and biochemical
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Chem.Pathol.Pharmacol. – 1993.- V. 80. - P. 187-200.
17 Kechrid Z., Dahdouh F., DjabarR.M., Bouzerna N. Combined effect of water contamination with cobalt and nickel on metabolism of albino
(wistar) rats // Iran J. Environ. Health Sci. Eng. –2006. - V. 3. – P. 65-69.
18 Costa M., Salnikow K., Cosentino S., Klein C.B., Huang X., Zhuang Z. Molecular mechanism of nickel carcinogenesis // Environ. Health. Perspect. –1994. – V. 102, 3. – P. 127-130.
19 Adedara I.A., Teberen R., Ebokaiwe A.P., Ehwerhemuepha T., Farombi E.O. Induction of oxidative stress in liver and kidney of rats exposed to Nigerian bonny light crude oil // Environ. Toxicol. –2011. – Doi: 10.1002/tox.20660.
20 Pari L., Prasath A. Effi cacy of caffeic acid in preventing nickel induced oxidative damage in liver of rats // Chem. Biol. Interact. - 2008.- V. 173. – P. 77-83.
21 Sidhu P., Garg M.L., Dhawan D.K. Protective role of zinc in nickel induced hepatotoxicity in rats // Chem. Biol. Interact. – 2004. – Vol. 150. – P. 199-209.
22 Elshaari F.A, Elshaari FA, Hadad G., Barassi I.F. Effect of Sodium Vanadate on Liver Function of Experimental Rats // Journal of Basic Medical and Allied Sciences (JBMAS). – 2011. – V. 1. – P. 5-10.
23 Russanov E., Zaporowska H., Ivancheva E., Kirkova M., Konstantinova S. Lipid peroxidation and antioxidant enzymes in vanadate-treated rats // Comp.Biochem. Physiol. Pharmacol. Toxicol. Endocrinol. –1994. – V. 107. – P. 415-421.
24 Misra M., Rodriguez R.E., Kasprazak K.S. Nickel induced lipid peroxidation in the rat: correlation with nickel effect on antioxidant defense system // Toxicol. - 1990. – V. 64. – P. 1-17.
25 Freeman B.A., Crapo J.D. Biology of disease: free radicals and tissue injury // Lab. Invest. – 1982. - V. 47. – № 5. – P. 412-426.
26 Bucker E.R., Martin S.E. Superoxide dismutase activity in thermally stressed Staphylococcus aureus // Appl. Environ. Microbiol. – 1981. – V. 41. – P. 449-454.
27 Chelikani P., Fita I., Loewen P.C. Diversity of structures and properties among catalases // Cell Mol. Life Sci. – 2004. – V. 61. – № 2. – P. 192-208.
28 Bray T.M., Taylor C.G. Tissue glutathione, nutrition, and oxidative stress // Can. J. Physiol. Pharmacol. – 1993. – V. 71. – № 9. – P. 746-751.
29 Chen L.H., Lin S.M. Modulation of acetaminophen-induced alterations of antioxidant defense enzymes by antioxidants or glutathione
precursors in cultured hepatocytes // Biochem. Arch. – 1977. – V. 13. – № 2. – P. 113-125.
30 Strange R.C., Fryer A.A. The glutathione-Stransferases: infl uence of polymorphism on cancer susceptibility // IARC Sci. Publ. – 1999. – V. 148. – P. 231-249.
31 Xiao N.W., Liu X.H., Li W., Ge F. Effect of herbicide acetochlor on cytochrome P450 monooxygenases and GST of earthworms
Eiseniafetida // J. Environ. Sci. (China). – 2006. – V.18. – № 1. – P. 135-140.
1989. – V.45.
2 Agency for toxic substances and disease registry (ATSDR). Toxicological profi le for total petroleum hydrocarbons (TPH): Potential for human exposure. U.S. department of health and human services. – Atlanta, 1999. www.atsdr.cdc.gov/ToxProfi les/tp123.pdf
3 Diker U. Oil pollution toxicity assessment:M.Sc. thesis / Istanbul technical university & Institute of science and technology. – Istanbul, 2007.
4 Madany I.M., Raveendran E. Polycyclic aromatic hydrocarbons, nickel and vanadium in air particulate matter in Bahrain during the burning of
oil fields in Kuwait // Sci. Total Environ. – 1992. – V. 116. – P. 281-289.
5 Falahi-Ardakani A. Contamination of environment with heavy metals emitted from automotives // Ecotoxicol. Environ. Saf. – 1984. – V.8. – P. 152-161.
6 Sokolov S.M. Methodological aspects of assessing atmospheric contamination with metal aerosols in the vicinity of thermal power complexes //J. Hyg. Epidemiol. Microbiol. Immunol. – 1986. – V.30. – P. 249-254.
7 Ayres R.U. Toxic heavy metals: materials cycle optimization // Proc.Natl.Acad.Sci. USA. –1992. – V. 89. – P. 815-820.
8 Mahmoud K.E., Shalahmetova T.M., Deraz Sh., Umbayev B. Combined effect of vanadium and nickel on lipid peroxidation and selected parameters of antioxidant system in liver and kidney of male rat // Afr. J. Biotechnol. – 2011. – V. 10. – № 79. – P. 18319-18325.
9 Burlakova E.B., Аlesenko A.V., Molochkina E.M., Palmina N.P., Khrapova N.G. Bioantioxidants for radiation damage and malignant growth. –Moscow: Nauka. - 1975. – 214 p.
10 Marklund S. Distribution of CuZn superoxide dismutase and Mn superoxide dismutase in human tissues and extracellular fl uids // Acta Physiol. Scand. Suppl. – 1980. – V. 492. – P. 19 – 23.
11 Aebi H. Catalase in vitro // Methods Enzymol. – 1984. – V. 105. – P. 121-126.
12 Baker M.A., Cerniglia G.J., Zaman A.Microtiter plate assay for the measurement of glutathione and glutathione disulfi de in large numbers
of biological samples // Anal.Biochem. – 1990. – V.190. – P. 360 – 365.
13 Habig W.H., Pabst M.J., Jakoby W.B. Glutathione S-transferases: the first enzymatic step in mercapturic acid formation // J. Biol. Chem. – 1974. – V. 249. – P. 7130–7139.
14 Landu S., Everitt B.S. A handbook of statistical analyses using SPSS. – London: Chapman and Hall / CRC press LLC, 2004. – P. 337.
15 Raza M., Al-ShabanahO.A., El-Hadiyah T.M., Al-Majed A.A. Effect of prolonged vigabatrin treatment on hematological and biochemical
parameters in plasma, liver and kidney of Swiss albino mice // Sci.Pharmaceut. – 2002. – V. 70. - P.135-145.
16 Thompson K.H., McNeill J.H. Effect of vanadyl sulfate feeding on susceptibility to peroxidative change in diabetic rats // Res. Commun.
Chem.Pathol.Pharmacol. – 1993.- V. 80. - P. 187-200.
17 Kechrid Z., Dahdouh F., DjabarR.M., Bouzerna N. Combined effect of water contamination with cobalt and nickel on metabolism of albino
(wistar) rats // Iran J. Environ. Health Sci. Eng. –2006. - V. 3. – P. 65-69.
18 Costa M., Salnikow K., Cosentino S., Klein C.B., Huang X., Zhuang Z. Molecular mechanism of nickel carcinogenesis // Environ. Health. Perspect. –1994. – V. 102, 3. – P. 127-130.
19 Adedara I.A., Teberen R., Ebokaiwe A.P., Ehwerhemuepha T., Farombi E.O. Induction of oxidative stress in liver and kidney of rats exposed to Nigerian bonny light crude oil // Environ. Toxicol. –2011. – Doi: 10.1002/tox.20660.
20 Pari L., Prasath A. Effi cacy of caffeic acid in preventing nickel induced oxidative damage in liver of rats // Chem. Biol. Interact. - 2008.- V. 173. – P. 77-83.
21 Sidhu P., Garg M.L., Dhawan D.K. Protective role of zinc in nickel induced hepatotoxicity in rats // Chem. Biol. Interact. – 2004. – Vol. 150. – P. 199-209.
22 Elshaari F.A, Elshaari FA, Hadad G., Barassi I.F. Effect of Sodium Vanadate on Liver Function of Experimental Rats // Journal of Basic Medical and Allied Sciences (JBMAS). – 2011. – V. 1. – P. 5-10.
23 Russanov E., Zaporowska H., Ivancheva E., Kirkova M., Konstantinova S. Lipid peroxidation and antioxidant enzymes in vanadate-treated rats // Comp.Biochem. Physiol. Pharmacol. Toxicol. Endocrinol. –1994. – V. 107. – P. 415-421.
24 Misra M., Rodriguez R.E., Kasprazak K.S. Nickel induced lipid peroxidation in the rat: correlation with nickel effect on antioxidant defense system // Toxicol. - 1990. – V. 64. – P. 1-17.
25 Freeman B.A., Crapo J.D. Biology of disease: free radicals and tissue injury // Lab. Invest. – 1982. - V. 47. – № 5. – P. 412-426.
26 Bucker E.R., Martin S.E. Superoxide dismutase activity in thermally stressed Staphylococcus aureus // Appl. Environ. Microbiol. – 1981. – V. 41. – P. 449-454.
27 Chelikani P., Fita I., Loewen P.C. Diversity of structures and properties among catalases // Cell Mol. Life Sci. – 2004. – V. 61. – № 2. – P. 192-208.
28 Bray T.M., Taylor C.G. Tissue glutathione, nutrition, and oxidative stress // Can. J. Physiol. Pharmacol. – 1993. – V. 71. – № 9. – P. 746-751.
29 Chen L.H., Lin S.M. Modulation of acetaminophen-induced alterations of antioxidant defense enzymes by antioxidants or glutathione
precursors in cultured hepatocytes // Biochem. Arch. – 1977. – V. 13. – № 2. – P. 113-125.
30 Strange R.C., Fryer A.A. The glutathione-Stransferases: infl uence of polymorphism on cancer susceptibility // IARC Sci. Publ. – 1999. – V. 148. – P. 231-249.
31 Xiao N.W., Liu X.H., Li W., Ge F. Effect of herbicide acetochlor on cytochrome P450 monooxygenases and GST of earthworms
Eiseniafetida // J. Environ. Sci. (China). – 2006. – V.18. – № 1. – P. 135-140.
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Как цитировать
Mahmoud, K. E., Shalahmetova, T. M., & Umbayev, B. A. (2015). Кратковременное действие сырой нефти, ванадия и никеля на антиоксидантную защитную систему печени крыс. Егеуқұйрықтар бауырының антиоксиданттық қорғау жүйесіне шикі мұнай, ванадий жəне никельдің қысқа мерзімді əсері. Вестник КазНУ. Серия биологическая, 54(2), 39–44. извлечено от https://bb.kaznu.kz/index.php/biology/article/view/258
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ГИСТОЛОГИЯ, ЦИТОЛОГИЯ, КЛЕТОЧНАЯ БИОЛОГИЯ
