Влияние z-лигустилида на генерацию супероксид аниона и фосфорилирование cPLA2 в астроцитах при воздействии β-амилоида. Астроциттерінде супероксид анионның генерациясына Z-лигустилидтың əсері жəне β-Амилоидтың əсерінен cPLA2- нің фосфорлануы
Ключевые слова:
β-амилоид, Z-лигустилид, астроциты крыс, активные формы кислорода, супероксиддисмутаза, егеуқұйрықтың астроциттері, оттектің белсенді түрлері, суперок- сиддисмутаза,Аннотация
В настоящей работе было изучено влияние Z-лигустилида (бутилиден-4,5-дигидрофталид) на генерацию супероксид аниона и фосфорилирование cPLA2 в астроцитах крыс при воздействии β-Амилоида. Установлено, что Z-лигустилид ингибирует генерацию супероксид аниона, синтез которого индуцирует β-Амилоид. Показано, что Z-лигустилид снижает уровень фосфорилирования cPLA2, но при этом не оказывает токсического действия на астроциты Осы жұмыста Z-лигустилиданың (бутилиден-4,5-дигидрофталид) əсері суперкапсид анаионның генерациясына жəне β-Амилоидтың əсерімен cPLA2- нің егеуқұйрығының астроциттеріне фосфорлануы зерттелген. Z-лигустилид суперкапсид анионының генерациясын тежейтіндігі анықталды, бұл синтез индуцирует етеді β-Амилоид. Z-лигустилид cPLA2-тің фосфорилденуін төмендетеді , бірақ уландырғыш қасиетін астроциттерге тигізбейтіндігі көрсетілді.Библиографические ссылки
1 Vassar R. BACE1: the beta-secretase enzyme in Alzheimer’s disease // J. Mol. Neurosci. – 2004. - №23. - P.105-114.
2 Bernstein S.L., Wyttenbach T., Baumketner A., Shea J.-E., Bitan G., Teplow D.B., Bowers M.T. Amyloid β-protein: monomer structure and early aggregation states of Aβ42 and its pro19 alloform // J. Amer.
Chem. Soc. - 2005. -127. – P. 2075-2084.
3 Abramov A.Y., Duchen M.R. The role of an astrocytic NADPH oxidase in the neurotoxicity of amyloid beta peptides // Biol Sci. – 2005. -V. 360. – P. 2309-2314.
4 Park L., Zhou P., Pitstick R., Capone C., Anrather J., Norris E.H., Younkin L., Younkin S., Carlson G., McEwen B.S., Iadecola C. Nox2-derived radicals contribute to neurovascular and behavioral dysfunction
in mice overexpressing the amyloid precursor protein // Proc. Nat. Ac. Sci. - 2008. - V. 105. - P. 1347-1352.
5 Dineley K.T., Westerman M., Bui D., Bel K., Ashe K.H., Sweatt J.D. beta-Amyloid Activates the Mitogen-Activated Protein Kinase Cascade via Hippocampal alpha 7 Nicotinic Acetylcholine Receptors: In Vitro and In Vivo Mechanisms Related to Alzheimer’s Disease // J. Neurosci. - 2001. - V. 21. - P. 4125-4133.
6 Stephenson D., Rash K., Smalstig B., Roberts E., Johnstone E., Sharp J., Panetta J., Little S., Kramer R., Clemens J. Cytosolic phospholipase A2 is induced in reactive glia following different forms of neurodegeneration
// Glia. - 1999. - V. 27. - P. 110-128.
7 Sun G.Y., Horrocks L.A., Farooqui A.A. The roles of NADPH oxidase and phospholipases A2 in oxidative and infl ammatory responses in neurodegenerative diseases // J. Neurochem. - 2007. - V. 103. - P. 1-16.
8 Sun G.Y., Xu J., Jensen M.D., Simonyi A.Phospholipase A2 in the central nervous system: implications for neurodegenerative diseases // J. Lipid Res. - 2004. - V. 45. - P. 205-213.
9 Yan Yu, Jun-Rong Du, Chen-Yuan Wang and Zhong-Ming Qian. Protection against hydrogen peroxide-induced injury by Z-ligustilide in PC12 cells // Experimental brain research. - 2008. - V. 184. - P. 307-312.
10 Fa M., Orozco I.J., Francis Y.I., Saeed F., Gong Y., Arancio O. Preparation of oligomeric beta-amyloid 1-42 and induction of synaptic plasticity impairment on hippocampal slices // J. Vis. Exp. - 2010. - V. 41. - P.1884.
11 Zanetti M., d’Uscio L.V., Peterson T.E., Katusic Z.S., O’Brien T. Analysis of Superoxide Anion Production in Tissue // Hypertension. - 2005. - P. 65-72.
12 Bolt A. and Mahoney B. High-effi ciency blotting of proteins of diverse sizes following sodium dodecyl sulfate–polyacrylamide, gel electrophoresis // Analytical Biochemistry. – 1997. – V. 247. – P. 185-192.
13 Schägger H. Tricine–SDS-PAGE // Nature Protocols. -2006. - V. 1. - P. 16-22.
14 Li W., Murai Y., Okada E., Matsui K., Hayashi S., Horie M., Takano Y. Modifi ed and simplifi ed western blotting protocol: use of intermittent microwave irradiation (IMWI) and 5% skim milk to improve binding
specifi city // Pathol. Int. - 2002. - V. 52(3). - P. 234-238.
15 Dennis-Sykes C.A., Miller W.J., McAleer W.J. A quantitative Western Blot method for protein measurement // J. Biol. Stand. - 1985. - V. 13(4). – P. 309-314.
16 Van Kooten T.G., Klein C.L., Kirkpatrick C.J. Western blotting as a method for studying cell-biomaterial interactions: the role of protein collection // J.Biomed. Mater. Res. - 2001. - V. 54(3). - P. 385-389.
2 Bernstein S.L., Wyttenbach T., Baumketner A., Shea J.-E., Bitan G., Teplow D.B., Bowers M.T. Amyloid β-protein: monomer structure and early aggregation states of Aβ42 and its pro19 alloform // J. Amer.
Chem. Soc. - 2005. -127. – P. 2075-2084.
3 Abramov A.Y., Duchen M.R. The role of an astrocytic NADPH oxidase in the neurotoxicity of amyloid beta peptides // Biol Sci. – 2005. -V. 360. – P. 2309-2314.
4 Park L., Zhou P., Pitstick R., Capone C., Anrather J., Norris E.H., Younkin L., Younkin S., Carlson G., McEwen B.S., Iadecola C. Nox2-derived radicals contribute to neurovascular and behavioral dysfunction
in mice overexpressing the amyloid precursor protein // Proc. Nat. Ac. Sci. - 2008. - V. 105. - P. 1347-1352.
5 Dineley K.T., Westerman M., Bui D., Bel K., Ashe K.H., Sweatt J.D. beta-Amyloid Activates the Mitogen-Activated Protein Kinase Cascade via Hippocampal alpha 7 Nicotinic Acetylcholine Receptors: In Vitro and In Vivo Mechanisms Related to Alzheimer’s Disease // J. Neurosci. - 2001. - V. 21. - P. 4125-4133.
6 Stephenson D., Rash K., Smalstig B., Roberts E., Johnstone E., Sharp J., Panetta J., Little S., Kramer R., Clemens J. Cytosolic phospholipase A2 is induced in reactive glia following different forms of neurodegeneration
// Glia. - 1999. - V. 27. - P. 110-128.
7 Sun G.Y., Horrocks L.A., Farooqui A.A. The roles of NADPH oxidase and phospholipases A2 in oxidative and infl ammatory responses in neurodegenerative diseases // J. Neurochem. - 2007. - V. 103. - P. 1-16.
8 Sun G.Y., Xu J., Jensen M.D., Simonyi A.Phospholipase A2 in the central nervous system: implications for neurodegenerative diseases // J. Lipid Res. - 2004. - V. 45. - P. 205-213.
9 Yan Yu, Jun-Rong Du, Chen-Yuan Wang and Zhong-Ming Qian. Protection against hydrogen peroxide-induced injury by Z-ligustilide in PC12 cells // Experimental brain research. - 2008. - V. 184. - P. 307-312.
10 Fa M., Orozco I.J., Francis Y.I., Saeed F., Gong Y., Arancio O. Preparation of oligomeric beta-amyloid 1-42 and induction of synaptic plasticity impairment on hippocampal slices // J. Vis. Exp. - 2010. - V. 41. - P.1884.
11 Zanetti M., d’Uscio L.V., Peterson T.E., Katusic Z.S., O’Brien T. Analysis of Superoxide Anion Production in Tissue // Hypertension. - 2005. - P. 65-72.
12 Bolt A. and Mahoney B. High-effi ciency blotting of proteins of diverse sizes following sodium dodecyl sulfate–polyacrylamide, gel electrophoresis // Analytical Biochemistry. – 1997. – V. 247. – P. 185-192.
13 Schägger H. Tricine–SDS-PAGE // Nature Protocols. -2006. - V. 1. - P. 16-22.
14 Li W., Murai Y., Okada E., Matsui K., Hayashi S., Horie M., Takano Y. Modifi ed and simplifi ed western blotting protocol: use of intermittent microwave irradiation (IMWI) and 5% skim milk to improve binding
specifi city // Pathol. Int. - 2002. - V. 52(3). - P. 234-238.
15 Dennis-Sykes C.A., Miller W.J., McAleer W.J. A quantitative Western Blot method for protein measurement // J. Biol. Stand. - 1985. - V. 13(4). – P. 309-314.
16 Van Kooten T.G., Klein C.L., Kirkpatrick C.J. Western blotting as a method for studying cell-biomaterial interactions: the role of protein collection // J.Biomed. Mater. Res. - 2001. - V. 54(3). - P. 385-389.
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ГИСТОЛОГИЯ, ЦИТОЛОГИЯ, КЛЕТОЧНАЯ БИОЛОГИЯ
