THE EFFECT OF THE FREEZING RATE ON THE QUALITY OF SPERM OF STURGEON FISH
DOI:
https://doi.org/10.26577/eb.2023.v96.i3.015Keywords:
cryopreservation, cryoprotector, stеrlet, stellate sturgeon, spermAbstract
This article discusses the issues of low-temperature preservation of sperm of sturgeon fish. The aim of the work is to study the effect of the freezing rate on the sperm quality indicators of sturgeon fish and the dependence of these indicators among themselves. The material for the research was the sperm of harvested males of sterlet and stellate sturgeon at the Ural-Atyrau sturgeon fish hatchery. When studying the effect of the freezing rate on the quality of sperm of sturgeon fish, optimal speeds were determined at the stages of stepwise freezing. When freezing sterlet sperm, the highest result was obtained at an optimal freezing rate of 170C/min with sperm motility of 76.8±1.25% and sperm lifetime of 216.2±1.44sec. The sperm of the male stellate sturgeon frozen in straws had a high rate at the beginning of freezing in the range of 15-480С/min, which affected the quality of the sperm of the stellate sturgeon, whose sperm motility ranged from 12.0-19.5%, the life time of the sperm was 228.5-373.75seconds. Relatively good results were obtained from the sperm of male stellate sturgeon frozen in Epindorf tubes, which had the highest freezing rate up to 200C/min. The motility of the sperms ranged from 27.75-44.75%, the lifetime of the sperms 393.25-674.5seconds. When studying, there is a direct relationship between the mobility and the lifetime of sperm of sturgeon fish, and also from the obtained research results it can be seen that the most optimal freezing rate of sperm of these species of sturgeon fish is a speed of up to 200C /min, the capacity in which it is advisable to freeze samples are Epindorf tubes, 0.5ml in volume, and the multi-stage freezing mode using special boxing.
References
Anan`ev, V.I. K voprosu o sozdanii nacional`noj sistemy` genofondny`x kollekcij ry`b i drugix gidrobiontov Rossii dlya akvakul`tury` i soxraneniya redkix i ischezayushhix vidov: pravovy`e i normativnometodologicheskie aspekty` / V.I. Anan`ev, M.S. Manoxina // Veterinarnaya patologiya. – 2007. – № 1. – S. 19-24.
Asturiano J.F., Cabrita E., Horvath A. Progress, challenges and perspectives on fish gamete cryopreservation: a mini-review. //Gen. Comp. Endocrinol. – 2017. – V. 245. – P. 69–76. https://doi.org/10.1016/j.ygcen.2016.06.019.
Bapsanova, A.M. Kriokonservaciya geneticheskogo materiala dlya soxraneniya redkix i ischezayushhix vidov zhivotny`x / A.M. Bapsanova // Mezhdunarodny`j zhurnal prikladny`x i fundamental`ny`x issledovanij. – 2012. – № 1. – S. 79.
Belaya M.M., Kraisl`nikova A.A. Vliyanie skorosti zamorazhivaniya na ry`bovodny`e kachestva spermy` osetrovy`x ry`b //Tovarnaya akvakul`tura i iskusstvennoe vosproizvodstvo gidrobiontov, //Vestnik AGTU. Ser.: Ry`bnoe xozyajstvo. 2019. №1. S.83-90 DOI: 10.24143/2073-5529-2019-1-83-90
Belaya M.M., Krasil`nikova A.A., Ponomareva E.N. Razrabotki yuzhnogo nauchnogo centra RAN v oblasti kriokonservacii reproduktivny`x kletok ry`b / Izvestiya Samarskogo nauchnogo centra Rossijskoj akademii nauk, t. 20, № 5(2), 2018 – S 280-286.
Belaya M.M., Krasil`nikova A.A., Ponomareva E.N. Razrabotki yuzhnogo nauchnogo centra RAN v oblasti kriokonservacii reproduktivny`x kletok ry`b / Izvestiya Samarskogo nauchnogo centra Rossijskoj akademii nauk, t. 20, № 5(2), 2018 – S 280-286.
Billard R., Cosson J., Crim L.W., Suquet M. //Sperm physiology and quality. In: Bromage, N.R., Roberts, R.J. (Eds.), Brood StockManagement and Egg and Larval Quality. Blackwell Science, London, England, 1995. - P. 25–52.
Chipinov, V.G. E`konomicheskaya e`ffektivnost` ispol`zovaniya kriokonservirovannoj spermy` pri vy`rashhivanii osetrovy`x vidov ry`b / V.G. Chipinov, M.M. Bogaty`reva // Aktual`ny`e problemy` obespecheniya prodovol`stvennoj bezopasnosti yuga Rossii: innovacionny`e texnologii dlya soxraneniya bioresursov, plodorodiya pochv, melioracii i vodoobespecheniya: materialy` Mezhdunarodnoj nauchnoj konferencii (27-30 sentyabrya 2011 g., Rostov-na-Donu). – Rostov-na-Donu: Izd-vo YuNCz RAN, 2011. – S. 133-135.
Ciereszko A, Judycka S, Nynca J, Słowinska M, Dietrich M. Factors influencing milt quality in fishes and its usefulness to cryopreservation. //Cryopreservation of fish gametes. – 2020. - P. 25-67. https://doi.org/10.1007/978-981-15-4025-7_3.
Cosson J., The ionic and osmotic factors controlling motility of fish spermatozoa. //Aquac. Int. – 2004. – V. 12. – P. 69–85. https://doi.org/10.1023/B:AQUI.0000017189.44263.bc.
Dokina O.B., Czvetkova L.I., Pronina L.D., Milenko V.A. Metod kriokonservacii spermy` osetrovy`x ry`b – ob``ektov akvakul`tury`. Akvakul`tura osetrovy`x ry`b: dostizheniya i perspektivy` razvitiya: Materialy` dokladov IV Mezhdunarodnoj nauchno-prakticheskoj konferencii, 13-15 marta 2006 g. Astraxan`. - M.: Izd-vo VNIRO, 2006, S.76-79
Dzyuba B. et al. Motility and fertilization ability of starlet Acipenserruthenus testicular sperm after cryopreservation // Cryobiology. – 2014. – V. 69. - P. 339-341.
Dzyuba B. et al. Spermatozoa motility, cryoresistance, and fertilizing ability in starlet Acipenserruthenus during sequential stripping // Aquaculture, 2012. – V. 356-357. – P. 272-278.
Horvath A., Chevre P., Urbanyi B. Sperm cryopreservation in sturgeon with a special focus on A. sturio: chap. 35 // In: Biology and conservation of the European sturgeon Acipensersturio L. 1758: the reunion of the European and Atlantic sturgeons. Ed. by Williot P., Rochard E., DesseBerset N., Kirschbaum F., Gessner J. /Heidelberg, NLD: Springer, 2011. – P. 465-476.
Huang B. X.-R. et al. Effect of cryopreservation on the enzyme activity of Russian sturgeon (Acipenser gueldenstaedtii Brandt &Ratzeburg, 1833) semen //J. Appl. Ichthyol. – 2014. – V. 30. - P. 1585-1589.
Korosov A. V., Gorbach V. V. Komp`yuternaya obrabotka biologicheskix danny`x. –Petrozavodsk: Izd-vo PetrGU,– 2007, –76 s.
Lakin G.F. Biometriya - M.: Vy`ssh.shkola, 1990.-352 s.
Magyary I., Urbányi B., Horváth Á., Dinnyes A. Cryopreservation of gametes and embryos of Cyprinid fishes. Cryopreservation in Aquatic Species, 2 ndEd. Ed. Tiersch T.R., Green Ch.C. World Aquaculture Society. Baton Rouge, Louisiana, USA. 2011. P. 525-538.
Martinez-Paramo S., Horvath A., Labbe C., Zhang T., Robles V., Herraez P., Suquet M., Adams S., Viveiros A., Tiersch T.R., Cabrita E. Cryobanking of aquatic species. //Aquaculture, 2016. – V. 472. – P.156–177. https://doi.org/10.1016/j.aquaculture.2016.05.042.
Matishov G.G., Ponomareva E.N., Belaya M.M. Soxranenie geneticheskogo raznoobraziya ry`b metodami nizkotemperaturnogo konservirovaniya // Ry`bnoe xozyajstvo. 2012. № 3. S. 59-62
Matrosova I. V. Biologicheskie osnovy` ry`bovodstva: e`kologo-gistofiziologicheskij podxod : uchebnoe posobie / I. V. Matrosova. — Naxodka: Dal`ry`bvtuz, 2020. — 79 s. — URL: https://e.lanbook.com/book/156844
Ponomareva E.N., Bogaty`reva M.M., Antonova N.A., Osipova V.P. Optimizaciya processa kriokonservacii spermy` osetrovy`x ry`b pri ispol`zovanii razlichny`x sred //Izvestiya Samarskogo nauchnogo centra Rossijskoj akademii nauk, t.11, №1(2), 2009. – S.132-134
Ponomareva E.N., Krasil`nikova A.A., Tixomirov A.M., Firsova A.V. Novy`e biotexnologicheskie metody` kriokonservacii reproduktivny`x kletok osetrovy`x vidov ry`b //E`kologiya zhivotny`x, t.11, №1, 2016– S.59-68
Sadeghi A. et al. Cryopreservation of stellate (Acipenserstellatus) sperm: Effect of diff erent concentrations of DMSO and dilution dates on sperm mobility and motility duration after longterm storage // Global Veterinaria. — 2013. – V. 10. – P. 26-30.
Vasil`eva L.M., Smirnova N.V., Yusupova A.Z. K voprosu soxraneniya i vosstanovleniya zapasov osetrovy`x ry`b v Volgo-Kaspijskom bassejne // Yug Rossii: e`kologiya, razvitie. 2012. T. 7, N1. S. 73-76. DOI:10.18470/1992-1098-2012-1-73-76
Xodorevskaya R.P., Kalmy`kov V.A., Zhilkin A.A. Sovremennoe sostoyanie zapasov osetrovy`x Kaspijskogo bassejna i mery` po ix soxraneniyu. Vestnik AGTU. Seriya: Ry`bnoe xozyajstvo. 2012. № 1. S.99-106
