Study of genetic diversity potato samples microsatellite markers
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Keywords:
potato variet, DNA, SSR- marker locus, allele,Abstract
We used the following methods: isolation and purification of DNA from potato, quantification and purity of the isolated DNA, DNA amplification primers, SSR- polyacrylamide gel electrophoresis, the construction of a dendrogram using UPGMA algorithm and a computer program TREECON. The studies used 7 pairs of microsatellite primers to assess polymorphism 48 grades of Kazakhstani and foreign selection, which together generate 41 amplification fragments ranging in size from 111 to 244 bp. Polymorphism loci studied was quite high and amounted to 97.6% . On the basis of SSR- alleles method of cluster analysis were identified genetic distances between 48 genotype of potatoes and tree was constructed.References
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2 Ghislain M., Nunez J., Rosario Herrera M., Pignataro J., Guzman F., Bonierbale M., Spooner D.M. Robust and highly informative microsatellite-based genetic identity kit for potato //Molecular Breeding. - 2009. - Vol. 23. - P. 377-388.
3 Edwards S.K., Johnstone C., Thompson C. Simple and rapid method for the preparation of plant genomic DNA for PCR analysis // Nucleic Acids Res. – 1991. - Vol. 19. - № 6. - P.1349.
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Kakimzhanova, A. A., & Esimseitova, A. K. (2015). Study of genetic diversity potato samples microsatellite markers. Experimental Biology, 60(2), 217–221. Retrieved from https://bb.kaznu.kz/index.php/biology/article/view/164
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BIOTECHNOLOGY
