GENOTYPING OF CAMELS OF ALMATY REGION BY CSN2 DAIRY PRODUCTIVITY GENE
DOI:
https://doi.org/10.26577/eb.2021.v87.i2.10Abstract
Camel milk is highly valued for its medicinal properties and high nutritional value. Camel milk and its products are easily digestible and therefore natural for the human body. Thus, the study of genetic diversity and selection of camels according to "preferred" genotypes is an important component of camel breeding in Kazakhstan.
The β-casein protein, encoded by the CSN2 gene, makes up the largest part of the casein fraction of camel milk and is considered the main gene for the presence of alleles associated with different levels of expression. The g.2126A> G transition is located the TATA box region of the CSN2 gene promoter, with a presumed effect on the transcription factor binding activity, which determines the intensity of this gene expression. In the studied camel populations bred in the Almaty region, the total number of which is 53 animals, the frequency of the G allele was 0.36, while the frequency of the A allele was 0.64. Also, the Hardy-Weinberg equilibrium was equal to 0,006. The level of polymorphism showed a high result in populations 1, 2 and 4, amounting to 100%, while in the third population this indicator was 66.67%. For the studied populations, such statistical indicators were identified as the genotypes occurrence frequency, the observed and effective number of alleles, Nei's gene diversity etc., which give the population genetic characteristics of populations.
References
2. https://www.kazportal.kz/verblyudovodstvo-v-kazahstane/
3. Berhe T., Seifu E., Ipsen R., Kurtu M.Y., Hansen, E.B. Processing challenges and opportunities of camel dairy products // Int. J. Food Sci. - 2017. - Vol. 2. - P. 1–8.
4. Pauciullo A., Giambra I., Iannuzzi L., Erhardt G. The β-casein in camels: molecular characterization of the CSN2 gene, promoter analysis and genetic variability // Gene. - 2014. - Vol. 547. - P. 159–168.
5. Pauciullo A., Shuiep E.S., Cosenza G., Ramunno L., Erhardt G. Molecular characterization and genetic variability at β-casein gene (CSN3) in camels // Gene. - 2013. - Vol. 513. - P. 22-30.
6. Ryskaliyeva A., Henry C., Miranda G., Faye B., Konuspayeva G., Martin P. Alternative splicing events expand molecular diversity of camel CSN1S2 increasing its ability to generate potentially bioactive peptides // Sci. Rep. - 2019. - Vol. 9. - P. 5243.
7. Avila F., Baily M.P., Perelman P., Das P.J., Pontius J., Chowdhary R. et al. A comprehensive whole-genome integrated cytogenetic map for the alpaca (Lama pacos) // Cytogenet. Genome Res. - 2014a. - Vol. 144. - P 196–207.
8. Pauciullo A, Shuiep E.T., Ogah M.D., Cosenza G., Di Stasio L., Erhardt G. Casein Gene Cluster in Camelids: Comparative Genome Analysis and New Findings on Haplotype Variability and Physical Mapping // Front. Genet. - 2019. - Vol. 10, - No 748.
9. Amigo L., Recio I., Ramos M. Genetic polymorphism of ovine milk proteins: its influence on technological properties ofmilk – a review // Int. Dairy J. - 2000. -Vol. 10. - P. 135–149.
10. Soyudal B., Ardicli S., Samli H., Dincel D., Balci F. Association of polymorphisms in the CSN2, CSN3, LGB and LALBA genes with milk production traits in Holstein cows raised in Turkey // J. Hellenic Vet. Med. Soc. - 2018. - Vol. 69. - P. 1271–1282.
11. Corral J., Padilla J., Izquierdo M. Associations between milk protein genetic polymorphisms and milk production traits in Merino sheep breed // Livest. Sci. - 2010. - V. 129. - P. 73–79.
12. Lee S.M., Kim H.M., Moon S.J., Kang M.J. Cloning and molecular characterization of porcine β-casein gene (CNS2) // Asian-Austral. J. Anim. - 2012. - V. 25. - P. 421–427.
13. Chessa S., Rignanese D., Berbenni M., Ceriotti G., Martini M., Pagnacco G., Caroli A. New genetic polymorphisms within ovine β- and αs2-caseins // Small Ruminant Res.- 2010. - Vol. 88. - P. 84-88.
14. Cosenza G., Pauciullo A., Colimoro L., Mancusi A., Rando A., Di Berardino D., Ramunno L. An SNP in the goat CSN2 promoter region is associated with the absence of β -casein in milk // Anim. Genet. - 2007. - Vol. 38. - P. 655-658.
15. Caroli A.M., Chessa S., Erhardt G.J. Milk protein polymorphisms in cattle: effect on animal breeding and human nutrition // J. Dairy Sci. - 2009. - Vol. 92. - P. 5335-5352.
16. Suteu M., Vlaic A., Daraban S.V. Characterization of a novel porcine CSN2 polymorphism and its distribution in five European breeds // Animals. - 2019. - Vol. 9. - No 7. - P. 419.
17. Novier A.M., Ramadan Sh. I. Association of β-casein gene polymorphism with milk composition traits of Egyptian Maghrebi camels (Camelus dromedarius) // Arch. Anim. Breed. - 2020. - Vol. 63. - P. 493–500.
18. Амандыкова М.Д., Досыбаев К.Ж., Байбагысов А.М., Литус И.А., Икласов М.К., Мусаева А.С., Бекманов Б.О., Сайто Н. Чacтoтa pacпpeдeлeния гeнa CSN3 y вepблюдoв Aлмaтинcкoй oблacти // Вестник КазНУ. Серия биологическая. - 2019. - Т. 81, № 4. - С. 34-42.
19. https://docplayer.ru/63720103-Instrukciya-dnk-sorb-s-m.html
20. Бекманов Б.О., Амиргалиева A.С., Мусаева А.С., Оразымбетова З.С., Досыбаев К.Ж., Хусаинова Э.М., Жапбасов Р.Ж., Жомартов A.M., Тулекей М.Д. Молекулярно-генетический анализ овец Едилбайской породы // Известия НАН РК. - 2015. № 3. - С. 28-33.
21. Nagy P., Fabri Zs.N., Varga L., Reiczigel J., Juhasz, J. Effect of genetic and nongenetic factors on chemical composition of individual milk samples from dromedary camels (Camelus dromedarius) under intensive management // J. Dairy Sci. - 2017. - V. 100. - P. 8680–8693.
22. Motoo Kimura, James F. Crow The number of alleles that can be maintained in a finite population // Genetics. - 1964. - Vol. 49. No 4. - P. 725-738.
23. Masatoshi Nei Analysis of gene diversity in subdivided populations // PNAS. - 1973. - V. 70 . - No 12., P. 3321-3323.
24. Lewontin R.C. The Apportionment of Human Diversity. In: Dobzhansky T., Hecht M.K., Steere W.C. (eds) // Evolutionary Biology. Springer. - 1972.
25. Смекенов И.Т., Акишев Ж.Д., Алтыбаева Н.А., Мухитдинов Н.М., Бисенбаев А.К. Оценка генетического полиморфизма популяции Berberis iliensis Или-Балхашского региона на основе ISSR-маркеров // Доклады Национальной Академии наук Республики Казахстан. - 2012. - № 4. - C. 49-57.
26.https://www.britannica.com/science/evolution-scientific-theory/Genetic-differentiation-during-speciation#ref311720