ASSESSMENT OF THE CYTOGENETIC CONSEQUENCES OF THE IMPACT OF UNUTILIZED AND BANNED TO USE PESTICIDES ON THE SHEEP ORGANISM
DOI:
https://doi.org/10.26577/eb.2021.v86.i1.09Abstract
In the territories of those farms in the Almaty region, where previously engaged in vegetable growing, fruit growing and tobacco growing are old, abandoned warehouse where accumulated considerable amount unutilized and banned pesticides. These warehouses are located directly on the territories of settlements. There is no sanitary zone around them. The chemical constituents of non-utilized pesticides constantly pollute the environment and with the products of animal and vegetable origin produced in the same settlements, enter the body.
The article is devoted to the cytogenetic assessment of the genotoxicity of unused and forbidden to use pesticides on the body of sheep. Animals are bred on pasture plots and kept in the settlements of Beskaynar, Kyzylkairat and Taukaraturyk, where old warehouses with pesticide.
A cytogenetic analysis of chromosome preparations prepared from an in vitro culture of peripheral blood cells from 30 sheep was performed. The level of cells with genomic mutations in animals from three experimental sites was 2.6 2.96 and 2.75 times higher, respectively, in comparison with the corresponding indices of control animals. An analysis of the constituent genomic cell mutations in the blood system of these animals showed that cells with hyperdiploid sets of chromosomes were identified in 17 animals with a frequency from 0.67% to 2.88% of the analyzed metaphase cells. Polyploid cells were found in all animals, with an average frequency of 7.93 ± 1.21% (3484 cells). The level of cells with chromosomal aberrations in experimental animals is 6.25, 7.40 and 5.66 times higher than in control animals. Analysis of the level of cytogenetic instability in animal cells showed that in the blood system of experimental animals, the genotoxicity of unused and forbidden pesticides is detected due to an increase in the proportion of cells with hyperdiploid, polyploid sets of chromosomes and cells with chromosome aberrations.
Key words: pesticides that are not utilized and banned to use, sheep, in vitro lymphocyte culture, genomic mutations, chromosomal aberrations.
References
2 A. A. Nurzhanova, Z. A. Inelova, L. B. Djansugurova, S. G. Nesterova, N. V. Mit, A. A. Zhubanova, R. Zh. Zhapbasov, M. Kh. Baizhanov, U. N. Kapysheva , Sh. K. Bakhtiyarova, E. M. Khussainova, O. G. Cherednichenko, A. S. Mussayeva, E. A. Shadenova, B. O. Bekmanov. The problem of unutilized and banned pesticides in Kazakhstan (review). News of the National Academy of Sciences of the Republic of Kazakhstan. Seriesofbiologicalandmedical. Volume 4, Number 328 (2018), 86 – 96.
3 Kozak M. F. Marchenko N. V. Tsitogeneticheskiye effekty vozdeystviya antropogennogo zagryazneniya vod nizhney Volgi. Monografiya. Astrakhan. Izdatelskiy dom «Astrakhanskiy universitet». 2008.116 str.
4 S.Zh. Kolumbayeva. D.A. Begimbetova. A.V. Lovinskaya. A.M. Kalimagambetov. Issledovaniye Sitellus fulvus v zagryaznennykh fenilpirazolami biotopakh.Ekologiya. 2013. № 3. s. 216–220.
5 Gershenzon S.M. Mutatsii. Kiyev. 1991. – S. 111-125.
6 Tarasov V. A. Printsipy kachestvennoy i kolichestvennoy otsenki geneticheskoy opasnosti khimicheskikh veshchestv. Mutageny i kantserogeny okruzhayushchey sredy i nasledstvennost cheloveka: doklady mezhdunarodnogo simpoziuma. M.. 1994. S. 3-66.
7 Begimbetova D.A.. Kolumbayeva S.Zh.. Blansho E.. Lovinskaya A.V. Izucheniye soderzhaniya fipronil-sulfona v tkanyakh krys pri ostrom i podostrom vozdeystvii. Vestnik Kaznu. Seriya ekologicheskaya. № 1 (24) 2009 g. str. 59-64.
8 A. V. Lovinskaya. S. Zh. Kolumbayeva. O. L. Kolomiyets. S. K. Abilev. Genotoksicheskoye deystviye pestitsida fipronila na somaticheskiye i generativnyye kletki myshey. Genetika. 2016. tom 52. № 5. s. 561–568
9 E.L. Levitskiy. A.N. Marchenko. Yu.I.Gubskiy. Mekhanizmy genotoksichnosti fosfororganicheskikh soyedineniy// Sovremennyye problemy toksikologii.-1998.-№1.-S.47-50.
10 Preeti Bagri & S. K. Jain. Assessment of acetamiprid-induced genotoxic effects in bone marrow cells of Swiss albino male mice // Drug and Chemical Toxicology – 2018. Vol. 42, No 4, – P. 357-363.
11 D. Medina-Buelvas, E. Estrada-Muñiz, M. Flores-Valadez, L. Vega. Genotoxic and immunotoxic effects of the organophosphate metabolite T diethyldithiophosphate (DEDTP) in Vivo // Toxicology and Applied Pharmacology – 2019. Vol. 366, – P. 96-103.
12 Doha Yahia, Marwa F. Ali. Cytogenetic and genotoxic effects of penconazole and chlorpyrifos pesticides in bone marrow of rats // Journal of Advanced Veterinary Research – 2019. – Vol. 9, No 2, – P. 29-38
13 Doha Yahia, Marwa F. Ali, Doaa S. Abd El-Maguid. Estimation of Bone Marrow DNA Damage Induced by Lambda cyhalothrin and Dimethoate Insecticides using Alkaline Comet Assay // Journal of Advanced Veterinary Research – 2019. – Vol. 9, No 1, – P. 23-28
14 Ayla Çelik, Gizem Güler, Cuma Aktaş & Serap Yalin. Genotoxic action of Luna Experience-SC 400 fungicide on rat bone marrow // Biomarkers – 2019. Vol. 24, No 7, – P. 720-725.
15 Tsatsakis A, Docea AO, Constantin C, Calina D, Zlatian O, Nikolouzakis TK, Stivaktakis PD, Kalogeraki A, Liesivuori J, Tzanakakis G, Neagu M. Genotoxic, cytotoxic, and cytopathological effects in rats exposed for 18 months to a mixture of 13 chemicals in doses below NOAEL levels // Toxicology Letters – 2019. Vol. 316, – P. 154-170.
16 P.K. Malhi and I.S. Grover. Genotoxic effects of some organophosphorus pesticides. II. In vivo chromosomal aberration bioassay in bone marrow cells in rat // Mutation Research – 1987. Vol. 188, – P. 45-51.
17 Ilyushina N. A. Egorova O.V.. Averianova N.S.. Masaltsev G.V.. Rakitskiy V.N. Genotoksichnost modelnykh kombinatsiy deystvuyushchikh veshchestv pestitsidov v testakh na bakteriyakh Salmonella typhimurium i eritrotsitakh kostnogo mozga myshey in vivo // Zdravookhraneniye Rossiyskoy Federatsii – 2019. – Tom 63. № 4. – C. 193-198.
18 Katarína Šiviková, Beáta Holečková, Viera Schwarzbacherová, Martina Galdíková, Ján Dianovský, Potential chromosome damage, cell-cycle kinetics/and apoptosis induced by epoxiconazole in bovine peripheral lymphocytes in vitro // Chemosphere Volume – 2018. Vol. 193, – P. 82-88.
19 Martina Galdíková, Beáta Holečková, Katarína Šiviková, Viera Schwarzbacherová, Simona Koleničová. Evaluating the genotoxic damage in bovine whole blood cells in vitro after T exposure to thiacloprid // Toxicology in Vitro – 2019. Vol. 61.
20 Ficová, I., Galdíková, M. Testing the potential clastogenic/ cytotoxic effects of pesticide calypso 480 scfolia veterinaria, 61, 3: 47–51, 2017.
21 BeátaHolečková. Evaluation of the in vitro effect of glyphosate-based herbicide on bovine lymphocytes using chromosome painting// Bull Vet InstPulawy 50, 533-536, 2006.
22 M.B. Lioi, M.R. Scarfı, A. Santoro, R. Barbieri, O. Zeni, D. Di Berardino, M.V. Ursini.Genotoxicity and oxidative stress induced by pesticide exposure in bovine lymphocyte cultures in vitro//Mutation Research 403 1998 13–20.
23 O vnesenii izmeneniy v prikaz Ministra selskogo khozyaystva Respubliki Kazakhstan ot 24 iyunya 2015 goda № 15-1/565 «Ob utverzhdenii standarta gosudarstvennoy uslugi «Gosudarstvennaya registratsiya pestitsidov (yadokhimikatov)».
24 Rukovodstvo p o kratkosrochnym testam dlya vyyavleniya mutagennykh i kantserogennykh khimicheskikh veshchestv. Podgotovleno dlya MPKhB Mezhdunarodnoy komissiyey po zashchite ot mutagenov i kantserogenov okruzhayushchey sredy. Sovmestnoye izdaniye Programmy OON po okruzhayushchey srede. Mezhdunarodnoy organizatsii truda i Vsemirnoy organizatsii zdravookhraneniya. Zheneva. 1989.-211 s.
25 Zhapbasov R.. Zhansugіrova L.B.. Zhomartov A.M.. Dosybayev ?.Zh. «Sutkorektі zhanuarlardyn somatikalyk kletkalaryndagy tsitogenetikalyk turaksyzdyk dengeyіn korshagan ortanyn ekologiyalyk zhagdayyna genotoksikalyk turgydan sipattama beruge paydalanu» // Adіstemelіk nuskau. – Almaty Kazak universitetі. 2017. – 74 bet.
26 Vasilyev S.A.. Timoshevskiy V.A.. Lebedev I.N. Aneugennyy effekt ioniziruyushego izlucheniya v somaticheskikh kletkakh mlekopitayushchikh i cheloveka. / Genetika. 2009. T.45. N 12. S.1589-1599.
27 Ryabokon N.I. Biologicheskiye effekty v prirodnykh populyatsiyakh melkikh gryzunov na territoriyakh. zagryaznennykh radionuklidami. Chastota poliploidnykh kletok kostnogo mozga u ryzhey polevki v raznyye gody posle Chernobylskoy katastrofy / Radiatsionnaya biologiya. Radioekologiya. – 1999. T. 39. №6. S. 613-618.
