Development of technology plant cultivation tau-sagyz (Scorzonera tau-saghyz Lipsch. et Bosse) using soil microflora
4. Mycorrhization in a laboratory experiment
DOI:
https://doi.org/10.26577/eb-2018-3-1346Abstract
Scorzonera tau-saghyz - endemic of Kazakhstan, capable of accumulate up to 40% of rubber in dry roots and quality is not inferior to rubber hevea. Currently, research work is underway to restore the number of this species, as well as to develop effective and cost-effective biotechnologies for obtaining commercial rubber from the roots of the tau-saghyz.
In long-term laboratory experiment carried out mycorrhization of seedlings. As inoculum used fungi Claroideoglomus etunicatum (Schüssler/Walker), Claroideoglomus claroideum (Schüssler/Walker) and Rhizophagus intraradices (Schüssler/Walker). In the roots of infected plants were revealed structures typical for fungi, forming arbuscular mycorrhizae type: non septate mycelium, vesicles and arbuscules.
Mycorrhizal infection in the studied samples of S. tau-saghyz roots was 100 %. Infected plants was noticeably better than non-mycorrhizal. The average height and number of leaves of mycorrhizal plants was 1.5 times higher than that of non-mycorrhizal plants, which is proof of the importance of arbuscular mycorrhizae in the life of stenotopic, rare and extinct S. tau-saghyz.
The use of endomycorrhizal fungi can significantly contribute to the development of modern, cost-effective biotechnologies aimed at restoring the abundance in nature and obtaining commercial rubber from the roots of tau-saghyz. These studies are particularly relevant at growing demand in the world for natural rubber and the search for alternative sources of natural rubber, in contrast to Hevea.
Key words: Scorzonera tau-saghyz Lipsch. et G.G. Bosse, Glomeromycota, Claroideoglomus etunicatum (Schüssler/Walker), Rhizophagus intraradices (Schüssler/Walker), Claroideoglomus claroideum (Schüssler/Walker).
References
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van Beilen J.B., Poirier Y. Establishment of new crops for the production of natural rubber. // Trends Biotechnol. – 2007. - №25. – Р. 522-529.
Mooibroek, H., Cornish, K., Alternative sources of natural rubber.// Appl. Microbiol. Biotechnol. - 2000. - №53. – Р. 355–365.
Павлов И.В. Растительные ресурсы Южного Казахстана. - М.: Московское общество испытателей природы, 1947. – 128 с.
Культиасов М.В. Тау-сагыз и введение его в культуру. - Ленинград, Издательство Академии наук СССР, 1938. - 315 с.
Богуспаев К.К., Адильбаев Ж.А., Фалеев Д.Г., Жанатаев Ж.А., Турашева С.К., Самбетов К.К. Перспективы разработки технологий восстановления популяции растений тау-сагыза (Scorzonera tau-saghyz Lipsch. et Bosse) в Каратауском государственном природном заповеднике // Вестник КазНУ, серия экологическая. - 2013. - №2/2, (38). - С. 64-70.
Селиванов И.А. Микосимбиотрофизм как форма консортивных связей в растительном покрове Советского Союза. - М., Наука, 1981. - 177 С.
Шнырева-Драга А.В. Взаимоотношения партнеров в симбиозе везикулярно-арбускулярного типа // Микология и фитопатология. - 1990. - Т. 24, Вып. 6. - С. 583-590.
Sharma A.K., Johri B.N. Arbusсular Mycorrhizae Interactions in Plants, Rizosphere and Soils. - Plymouth: Science Publichers UK, 2002. – 363 р.
Peterson L.R., Massicotte B., Lewis H.M. Mycorrhizas: Anatomy and Cell Biology – Ottava, National Research Council of Canada, 2004. – 173 p.
Smith S.E., Read D.J. Mycorrhizal symbiosis. - Third Edition. NY. Acad Press. - 2008. - 787 p.
Singh R.K., Gogoi P. Augmented growth of long pepper in response to arbuscular mycorrhizal inoculation. // J. Forest. Res. - 2012. - Vol. 23, № 2. - P. 339-344.
Shukla A., Kumar A., Jha A., Ajit.,Rao D.V.K. Nageswara. Phosphorus threshold for arbuscularmycorrhizal colonization of crops and tree seedlings // Biol. and Fert. Soils.- 2012. - Vol. 48, № 1. - P. 109-116.
Cozzolino V., Di M.V., Piccolo A. Impact of arbuscular mycorrhizal fungi applications on maize production and soil phosphorus availability. // J. Geochem. Explor. - 2013. - № 129. - P. 40-44.
Thompson J.P., Clewett T.G., Fiske M.L. Field inoculation with arbuscular-mycorrhizal fungi overcomes phosphorus and zinc deficiencies of linseed (Linum usitatissimum) in a vertisol subject to long-fallow disorder. // Plant and Soil. - 2013. - Vol. 371, № 1-2. - P. 117-137.
Soares A.C.F., da Silva S.C., da Silva G.M., de Sousa L.F. Fungos micorrizicos arbusculares no crescimento e nutricao de mudas de jenipapeiro. // Rev. cienc. agron. - 2012. - Vol. 43, № 1. - P. 47-54.
Umamaheswari N., Kannahi M., Selvaraj T. Effect of native AM fungi on growth, nutrition and biochemical constituents of Crotalaria juncea. // J. Ecobiol. - 2010. - Vol. 26, № 3-4. - P. 341-348.
Merrild M. P., Ambus P., Rosendahl S., Jakobsen I. Common arbuscular mycorrhizal networks amplify competition for phosphorus between seedlings and established plants. // New Phytol. - 2013. - Vol. 200, № 1. - P. 229-240.
Walder F., Niemann H., Natarajan M., Lehmann M. F., Boller T., Wiemken A. Mycorrhizal networks: Common goods of plants shared under unequal terms of trade. // Plant Physiol. - 2012. - Vol. 159, № 2. - P. 789-797.
Фалеев Д.Г. Арбускулярная микориза тау-сагыза (Scorzonera tau-saghyz Lipsch. et Bosse) Каратауского ГПЗ (Сырдарьинский Каратау). // Вестник КазНУ, серия экологическая. – 2014. - №1/1, (40). - С. 427-434.
Богуспаев К.К., Фалеев Д.Г., Касымбеков Б.К. Разработка технологии культивирования растений тау-сагыза (Scorzonera tau-saghyz Lipsch. et Bosse) с использованием почвенной микрофлоры: 1. Изучение микосимбиотрофизма в природе и в условиях культивирования // Вестник КазНУ. Серия биологическая. – 2014. - № 2 (61). – С. 41-50.
Blaszkowski J. Glomeromycota. – Krakow, W. Szafer Institute of Botany, Polish Academy of Sciences, 2012. – 303 p.
References
Blaszkowski J. (2012) Glomeromycota. Krakow: W. Szafer Institute of Botany, Polish Academy of Sciences, 303 p.
Boguspaev K.K., Adilbaev Zh.A., Faleyev D.G., Zhanataev Zh.A., Turasheva S.K., Sambetov K.K. (2013) Perspektivy razrabotki tehnologii vosstanovlenija populjacyi rastenii tau-sagyza (Scorzonera tau-saghyz Lipsch., Et Bosse) v Karatauskom gosudarstvennom prirodnom zapovednike [Prospects for the development of technologies for the restoration of the population of tau-sagyz plants (Scorzonera tau-saghyz Lipsch., Et Bosse) in the Karatau State Nature Reserve.]. KazNU Bulletin. Biology series. no 2 / 2, (38), pp. 64-70.
Boguspaev K.K., Faleyev D.G., Kasymbekov B.K. (2014) Razrabotka tehnologii kultivirovanija rastenii tau-sagyza (Scorzonera tau-saghyz Lipsch. et Bosse) s ispolzovaniem pochvennoi mikroflory: 1. Izuchenie mikosimbiotrofizma v prirode i v uslovijach kultivirovanija [Development of the technology of cultivation of tau-sagyz plants (Scorzonera tau-saghyz Lipsch, et Bosse) using soil microflora: 1. Study of mycosymbiotrophism in nature and under cultivation conditions]. KazNU Bulletin. Biology series. no 2 (61), pp. 41-50.
Cozzolino V., Di M.V., Piccolo A. (2013) Impact of arbuscular mycorrhizal fungi applications on maize production and soil phosphorus availability. J. Geochem. Explor. no 129, pp. 40-44.
Faleyev D.G. (2014) Arbuscularnaja mycoriza tau-sagyza (Scorzonera tau-saghyz Lipsch. Et Bosse) Karatauskogo GPZ (Syrdarinskii Karatau) [Arbuscular mycorrhiza of tau-sagyz (Scorzonera tau-saghyz Lipsch. Et Bosse) of the Karatau Gas Processing Plant (Syrdarya Karatau]. KazNU Bulletin. Ecology series. no 1/1, (40), pp. 427-434.
Gelling K. (2013) On the Rebound. Scientists revive search for new rubber sources. Science News. no 9, pp. 67-71.
Kultiazov M.V. (1938) Tau-sagyz and its introduction into culture [Tau-sagyz and its introduction into culture]. - Leningrad, Publishing house of the USSR Academy of Sciences. 315 p.
Merrild M.P., Ambus P., Rosendahl S., Jakobsen I. (2013) Common arbuscular mycorrhizal networks amplify competition for phosphorus between seedlings and established plants. New Phytol. vol. 200, no 1, pp. 229-240.
Mooibroek, H., Cornish, K., (2000) Alternative sources of natural rubber. Appl. Microbiol. Biotechnol. no 53, pp. 355–365.
Pavlov I.V. (1947) Rastitelnye resursy Juzhnogo Kazakhstana [Plant resources of Southern Kazakhstan]. – M.: Moskovskoe Obshestvo ispytatelei prirody. – 128 p.
Peterson L.R., Massicotte B., Lewis H.M. (2004) Mycorrhizas: Anatomy and Cell Biology – Ottava, National Research Council of Canada, 173 p.
Selivanov I.A. (1981) Mycosymbiotrophism kak forma konsortivnyh svjazei v rastitelnom pokrove Sovetskogo Sojuza [Mycosymbiotrophism as a form of consortium connections in the plant cover of the Soviet Union]. M., Nauka, 177 p.
Sharma A.K., Johri B.N. (2002) Arbusсular Mycorrhizae Interactions in Plants, Rizosphere and Soils. Plymouth: Science Publichers UK, 363 р.
Shnyreva-Draga A.V. (1990) Vzaimootnoshenija partnerov v simbioze vezikuljarno-arbuskuljarnogo tipa [Mutual relations of partners in symbiosis of the vesicular-arbuscular type. Mycology and phytopathology]. Mikologija i fitopatologija. vol. 24, no 6. pp. 583-590.
Shukla A., Kumar A., Jha A., Ajit. Rao D.V.K. Nageswara. (2012) Phosphorus threshold for arbuscularmycorrhizal colonization of crops and tree seedlings. Biol. and Fert. Soils. vol. 48, no 1, pp. 109-116.
Singh R.K., Gogoi P. (2012) Augmented growth of long pepper in response to arbuscular mycorrhizal inoculation. J. Forest. Res. Vol. 23, no 2, pp. 339-344.
Smith S.E., Read D.J. (2008) Mycorrhizal symbiosis. Third Edition. NY. Acad Press. 787 p.
Soares A.C.F., da Silva S.C., da Silva G.M., de Sousa L.F. (2012) Fungos micorrizicos arbusculares no crescimento e nutricao de mudas de jenipapeiro. Rev. cienc. agron. vol. 43, no 1, pp. 47-54.
Thompson J.P., Clewett T.G., Fiske M.L. (2013) Field inoculation with arbuscular-mycorrhizal fungi overcomes phosphorus and zinc deficiencies of linseed (Linum usitatissimum) in a vertisol subject to long-fallow disorder. Plant and Soil. vol. 371, no 1-2, pp. 117-137.
Umamaheswari N., Kannahi M., Selvaraj T. (2010) Effect of native AM fungi on growth, nutrition and biochemical constituents of Crotalaria juncea. J. Ecobiol. vol. 26, no 3-4, pp. 341-348.
van Beilen J.B., Poirier Y. (2007) Establishment of new crops for the production of natural rubber. Trends Biotechnol. no 25, pp. 522-529.
Walder F., Niemann H., Natarajan M., Lehmann M.F., Boller T., Wiemken A. (2012) Mycorrhizal networks: Common goods of plants shared under unequal terms of trade. Plant Physiol. –vol. 159, no 2, pp. 789-797.
van Beilen J.B., Poirier Y. Establishment of new crops for the production of natural rubber. // Trends Biotechnol. – 2007. - №25. – Р. 522-529.
Mooibroek, H., Cornish, K., Alternative sources of natural rubber.// Appl. Microbiol. Biotechnol. - 2000. - №53. – Р. 355–365.
Павлов И.В. Растительные ресурсы Южного Казахстана. - М.: Московское общество испытателей природы, 1947. – 128 с.
Культиасов М.В. Тау-сагыз и введение его в культуру. - Ленинград, Издательство Академии наук СССР, 1938. - 315 с.
Богуспаев К.К., Адильбаев Ж.А., Фалеев Д.Г., Жанатаев Ж.А., Турашева С.К., Самбетов К.К. Перспективы разработки технологий восстановления популяции растений тау-сагыза (Scorzonera tau-saghyz Lipsch. et Bosse) в Каратауском государственном природном заповеднике // Вестник КазНУ, серия экологическая. - 2013. - №2/2, (38). - С. 64-70.
Селиванов И.А. Микосимбиотрофизм как форма консортивных связей в растительном покрове Советского Союза. - М., Наука, 1981. - 177 С.
Шнырева-Драга А.В. Взаимоотношения партнеров в симбиозе везикулярно-арбускулярного типа // Микология и фитопатология. - 1990. - Т. 24, Вып. 6. - С. 583-590.
Sharma A.K., Johri B.N. Arbusсular Mycorrhizae Interactions in Plants, Rizosphere and Soils. - Plymouth: Science Publichers UK, 2002. – 363 р.
Peterson L.R., Massicotte B., Lewis H.M. Mycorrhizas: Anatomy and Cell Biology – Ottava, National Research Council of Canada, 2004. – 173 p.
Smith S.E., Read D.J. Mycorrhizal symbiosis. - Third Edition. NY. Acad Press. - 2008. - 787 p.
Singh R.K., Gogoi P. Augmented growth of long pepper in response to arbuscular mycorrhizal inoculation. // J. Forest. Res. - 2012. - Vol. 23, № 2. - P. 339-344.
Shukla A., Kumar A., Jha A., Ajit.,Rao D.V.K. Nageswara. Phosphorus threshold for arbuscularmycorrhizal colonization of crops and tree seedlings // Biol. and Fert. Soils.- 2012. - Vol. 48, № 1. - P. 109-116.
Cozzolino V., Di M.V., Piccolo A. Impact of arbuscular mycorrhizal fungi applications on maize production and soil phosphorus availability. // J. Geochem. Explor. - 2013. - № 129. - P. 40-44.
Thompson J.P., Clewett T.G., Fiske M.L. Field inoculation with arbuscular-mycorrhizal fungi overcomes phosphorus and zinc deficiencies of linseed (Linum usitatissimum) in a vertisol subject to long-fallow disorder. // Plant and Soil. - 2013. - Vol. 371, № 1-2. - P. 117-137.
Soares A.C.F., da Silva S.C., da Silva G.M., de Sousa L.F. Fungos micorrizicos arbusculares no crescimento e nutricao de mudas de jenipapeiro. // Rev. cienc. agron. - 2012. - Vol. 43, № 1. - P. 47-54.
Umamaheswari N., Kannahi M., Selvaraj T. Effect of native AM fungi on growth, nutrition and biochemical constituents of Crotalaria juncea. // J. Ecobiol. - 2010. - Vol. 26, № 3-4. - P. 341-348.
Merrild M. P., Ambus P., Rosendahl S., Jakobsen I. Common arbuscular mycorrhizal networks amplify competition for phosphorus between seedlings and established plants. // New Phytol. - 2013. - Vol. 200, № 1. - P. 229-240.
Walder F., Niemann H., Natarajan M., Lehmann M. F., Boller T., Wiemken A. Mycorrhizal networks: Common goods of plants shared under unequal terms of trade. // Plant Physiol. - 2012. - Vol. 159, № 2. - P. 789-797.
Фалеев Д.Г. Арбускулярная микориза тау-сагыза (Scorzonera tau-saghyz Lipsch. et Bosse) Каратауского ГПЗ (Сырдарьинский Каратау). // Вестник КазНУ, серия экологическая. – 2014. - №1/1, (40). - С. 427-434.
Богуспаев К.К., Фалеев Д.Г., Касымбеков Б.К. Разработка технологии культивирования растений тау-сагыза (Scorzonera tau-saghyz Lipsch. et Bosse) с использованием почвенной микрофлоры: 1. Изучение микосимбиотрофизма в природе и в условиях культивирования // Вестник КазНУ. Серия биологическая. – 2014. - № 2 (61). – С. 41-50.
Blaszkowski J. Glomeromycota. – Krakow, W. Szafer Institute of Botany, Polish Academy of Sciences, 2012. – 303 p.
References
Blaszkowski J. (2012) Glomeromycota. Krakow: W. Szafer Institute of Botany, Polish Academy of Sciences, 303 p.
Boguspaev K.K., Adilbaev Zh.A., Faleyev D.G., Zhanataev Zh.A., Turasheva S.K., Sambetov K.K. (2013) Perspektivy razrabotki tehnologii vosstanovlenija populjacyi rastenii tau-sagyza (Scorzonera tau-saghyz Lipsch., Et Bosse) v Karatauskom gosudarstvennom prirodnom zapovednike [Prospects for the development of technologies for the restoration of the population of tau-sagyz plants (Scorzonera tau-saghyz Lipsch., Et Bosse) in the Karatau State Nature Reserve.]. KazNU Bulletin. Biology series. no 2 / 2, (38), pp. 64-70.
Boguspaev K.K., Faleyev D.G., Kasymbekov B.K. (2014) Razrabotka tehnologii kultivirovanija rastenii tau-sagyza (Scorzonera tau-saghyz Lipsch. et Bosse) s ispolzovaniem pochvennoi mikroflory: 1. Izuchenie mikosimbiotrofizma v prirode i v uslovijach kultivirovanija [Development of the technology of cultivation of tau-sagyz plants (Scorzonera tau-saghyz Lipsch, et Bosse) using soil microflora: 1. Study of mycosymbiotrophism in nature and under cultivation conditions]. KazNU Bulletin. Biology series. no 2 (61), pp. 41-50.
Cozzolino V., Di M.V., Piccolo A. (2013) Impact of arbuscular mycorrhizal fungi applications on maize production and soil phosphorus availability. J. Geochem. Explor. no 129, pp. 40-44.
Faleyev D.G. (2014) Arbuscularnaja mycoriza tau-sagyza (Scorzonera tau-saghyz Lipsch. Et Bosse) Karatauskogo GPZ (Syrdarinskii Karatau) [Arbuscular mycorrhiza of tau-sagyz (Scorzonera tau-saghyz Lipsch. Et Bosse) of the Karatau Gas Processing Plant (Syrdarya Karatau]. KazNU Bulletin. Ecology series. no 1/1, (40), pp. 427-434.
Gelling K. (2013) On the Rebound. Scientists revive search for new rubber sources. Science News. no 9, pp. 67-71.
Kultiazov M.V. (1938) Tau-sagyz and its introduction into culture [Tau-sagyz and its introduction into culture]. - Leningrad, Publishing house of the USSR Academy of Sciences. 315 p.
Merrild M.P., Ambus P., Rosendahl S., Jakobsen I. (2013) Common arbuscular mycorrhizal networks amplify competition for phosphorus between seedlings and established plants. New Phytol. vol. 200, no 1, pp. 229-240.
Mooibroek, H., Cornish, K., (2000) Alternative sources of natural rubber. Appl. Microbiol. Biotechnol. no 53, pp. 355–365.
Pavlov I.V. (1947) Rastitelnye resursy Juzhnogo Kazakhstana [Plant resources of Southern Kazakhstan]. – M.: Moskovskoe Obshestvo ispytatelei prirody. – 128 p.
Peterson L.R., Massicotte B., Lewis H.M. (2004) Mycorrhizas: Anatomy and Cell Biology – Ottava, National Research Council of Canada, 173 p.
Selivanov I.A. (1981) Mycosymbiotrophism kak forma konsortivnyh svjazei v rastitelnom pokrove Sovetskogo Sojuza [Mycosymbiotrophism as a form of consortium connections in the plant cover of the Soviet Union]. M., Nauka, 177 p.
Sharma A.K., Johri B.N. (2002) Arbusсular Mycorrhizae Interactions in Plants, Rizosphere and Soils. Plymouth: Science Publichers UK, 363 р.
Shnyreva-Draga A.V. (1990) Vzaimootnoshenija partnerov v simbioze vezikuljarno-arbuskuljarnogo tipa [Mutual relations of partners in symbiosis of the vesicular-arbuscular type. Mycology and phytopathology]. Mikologija i fitopatologija. vol. 24, no 6. pp. 583-590.
Shukla A., Kumar A., Jha A., Ajit. Rao D.V.K. Nageswara. (2012) Phosphorus threshold for arbuscularmycorrhizal colonization of crops and tree seedlings. Biol. and Fert. Soils. vol. 48, no 1, pp. 109-116.
Singh R.K., Gogoi P. (2012) Augmented growth of long pepper in response to arbuscular mycorrhizal inoculation. J. Forest. Res. Vol. 23, no 2, pp. 339-344.
Smith S.E., Read D.J. (2008) Mycorrhizal symbiosis. Third Edition. NY. Acad Press. 787 p.
Soares A.C.F., da Silva S.C., da Silva G.M., de Sousa L.F. (2012) Fungos micorrizicos arbusculares no crescimento e nutricao de mudas de jenipapeiro. Rev. cienc. agron. vol. 43, no 1, pp. 47-54.
Thompson J.P., Clewett T.G., Fiske M.L. (2013) Field inoculation with arbuscular-mycorrhizal fungi overcomes phosphorus and zinc deficiencies of linseed (Linum usitatissimum) in a vertisol subject to long-fallow disorder. Plant and Soil. vol. 371, no 1-2, pp. 117-137.
Umamaheswari N., Kannahi M., Selvaraj T. (2010) Effect of native AM fungi on growth, nutrition and biochemical constituents of Crotalaria juncea. J. Ecobiol. vol. 26, no 3-4, pp. 341-348.
van Beilen J.B., Poirier Y. (2007) Establishment of new crops for the production of natural rubber. Trends Biotechnol. no 25, pp. 522-529.
Walder F., Niemann H., Natarajan M., Lehmann M.F., Boller T., Wiemken A. (2012) Mycorrhizal networks: Common goods of plants shared under unequal terms of trade. Plant Physiol. –vol. 159, no 2, pp. 789-797.
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Faleyev, D. G., Kasymbekov, B. K., Faleyev, E. G., Myrzagaliev, Z. Z., & Boguspaev, K. K. (2018). Development of technology plant cultivation tau-sagyz (Scorzonera tau-saghyz Lipsch. et Bosse) using soil microflora: 4. Mycorrhization in a laboratory experiment. Experimental Biology, 76(3), 142–151. https://doi.org/10.26577/eb-2018-3-1346
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