Biological activity of extracts from the roots of Vexibia alopecuroides and Salvia deserta
DOI:
https://doi.org/10.26577/eb-2018-1-1313Abstract
Biologically active compounds (BAC) of plants are represented by various classes of organic compounds and are a prospective raw material for developing therapeutic and preventive remedies. There are more than 6,000 plant species on the territory of the Republic of Kazakhstan that might become a source of raw materials for development and production of new domestic original phyto-pharmaceuticals. Therefore, testing of biologically active compounds extracted from Kazakhstan’s wild-growing plants is a timely and promising task.
It was determined that the roots of two wild plants of Kazakhstan flora - Salvia deserta and Vexibia alopecuroides contain biologically active compounds with high a) antibacterial and antifungicidal activities estimated by the IC50 index, b) anti-inflammatory activity estimated by the ability to inhibit production of nitric oxide, c) therapeutically significant antioxidant potential with respect to the ABTS.+ radical cations. Crude extracts of biologically active compounds from the roots of S. deserta and V. alopecuroides plants that have the above mentioned activities were obtained by maceration with methylene chloride, purified into individual compounds using flash and gas chromatography. It was determined that 9 individual compounds that revealed biological activity were isolated from Vexibia alopecuroides roots’ extract, which belongs to flavonoids, and 4 compounds were isolated from Salvia deserta roots’ extract, which belongs to diterpenoids.
Individual compounds isolated from Salvia deserta and Vexibia alopecuroides roots’ extract showed antimicrobial activity against S. aureus, Methicillin-resistant S. aureus, P. aeruginosa, C. glabrata, C. krusei, C. albicans with an IC50 index within the range of < 0, 8 to 13.38 μg / ml. It allows to consider them as potential candidates for development of new-galenic phyto-pharmaceuticals.
Key words: biologically active compounds, crude extracts, antimicrobial activity, Salvia deserta, Vexibia alopecuroides.
References
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4 Matkowski A., Piotrowska M. Antioxidant and free radical scavenging activities of some medicinal plants from the Lamiaceae // Fitoterapia. - 2006. - № 77. - Р. 346–353.
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7 Grover J.K, Yadav S.P. Pharmacological actions and potential uses of Momordica charantia: A review // Journal of Ethnopharmacology. - 2004. - Vol. 93. - Р.123–132.
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12 Grover J.K., Vats V., Rathi S.S. Medicinal plants of India with anti-diabetic potential // Journal of Ethnopharmacology. – 2000. - № 73. - Р. 461–470.
13 Степанова Э. Ф. Биотехнологические исследования по безотходному использованию солодки: автореф. дисс. д. фарм. н.: 15.00.01. - Харьков, 1989. - 47 с.
14 Павлов Н.В. Флора Казахстана - Алма-Ата: Академия наук Казахской ССР. - 1966. - Т.9. - 654 c.
15 Голосков В.Р. Иллюстрированный определитель растений Казахстана. - Алма-Ата: Наука. - 1969. - Т.1. - 243 с.
16 Музычкина Р.А., Корулькин Д.Ю., Абилов Ж.А. Основы химии природных соединений. - Алматы: Қазақ университеті. - 2010. - 564 с.
17 Sarker S. D., Latif Z., Gray A. I. Natural Products Isolation. - New York City: Human Press. -2005. - 515 p.
18 Clinical and Laboratory Standards Institute. Methods for dilution antimicrobial susceptibility test for bacteria that grow aerobically. – 2006, Seventh edition. - Document M7-A7. – 26 p.
19 Re R., Pellegrini N., Proteggente A. et al. Antioxidant activity applying an improved ABTS radical cation depolarization assay // Free Radical Biology and Medicine. - 1999. - Vol. 26. - № 9/10. - P. 1231-1237.
20 Patel A. et al. Estimation of Flavonoid, Polyphenolic Content and In-vitro Antioxidant Capacity of leaves of Tephrosia purpurea Linn. (Leguminosae) // International Journal of Pharma Sciences and Research. - 2010. - Vol.1. - N 1. - P. 66-77.
21 Пат. 2490628 РФ. Способ определения содержания аскорбиновой кислоты / Е.А. Бородин; опубл. 20.08.13, Бюл. № 23. – 4 с.
22 Chang W.T., Huang W.C., Liou C.J. Evaluation of the anti-inflammatory effects of phloretin and phlorizin in lipopolysaccharide-stimulated mouse macrophages // Food Chemistry. - 2012. - Vol.134. - P. 972-979.
23 Беленовская Л. М. Растительные ресурсы СССР: Цветковые растения, их химический состав, использование. cем. Hippuridaceae - Lobeliaceae. - М.: Наука. - 1991. - С.72-83.
24 Krishna P.M., KNV R., Sandhya S., Banji D. A review on phytochemical, ethnomedical and pharmacological studies on genus Sophora, Fabaceae // Revista Brasileira de Farmacognosia. – 2012. – Vol.22. – P.1145-1154.
25 Ulubelen A., Öksüz S., Topcu G., Gören A.C., Voelter W. Antibacterial Diterpenes from the Roots of Salvia b lepharochlaena // Journal of natural products. – 2001. – Vol. 64. – P. 549-551.
References
1 Bettaieb I., Hamrouni-Sellami I., Bourgou S. et al. (2011) Drought effects on the polyphenol composition and antioxidant activities in the aerial parts of Salvia officinalis L. ActaPhysiol Plant., no. 33, p. 1103-1111.
2 Biswas N.N., Saha S., Ali M. Kh. (2014) Antioxidant, antimicrobial, cytotoxic and analgesic activities of ethanolic extract of Mentha arvensis L. Asian Pacific. Journal of Tropical Biomedicine, no. 4 (10), p. 792-797.
3 Chang W.T., Huang W. C., Liou C.J. (2012) Evaluation of the anti-inflammatory effects of phloretin and phlorizin in lipopolysaccharide-stimulated mouse macrophages. Food Chemistry, vol. 134, p. 972-979.
4 Clinical and Laboratory Standards Institute. (2006) Methods for dilution antimicrobial susceptibility. Seventh edition, Document M7-A7, 26 p.
5 Genta S.B., Cabrera W.M., Mercado M. I., Grau A., Catalan C. A., Sanchez S.S. (2010) Hypoglycemic activity of leaf organic extracts from Smallanthus sonchifolius: Constituents of the most active fractions. Chemico-Biological Interactions, vol.185, p. 143-152.
6 Grover J.K, Yadav S.P. (2004) Pharmacological actions and potential uses of Momordica charantia: A review. Journal of Ethnopharmacology, vol. 93, p. 123-132.
7 Grover J.K., Vats V., Rathi S.S. (2000) Medicinal plants of India with anti-diabetic potential. Journal of Ethnopharmacology, no. 73, p. 461-470.
8 Krishna P.M., KNV R., Sandhya S., Banji D. (2012) A review on phytochemical, ethnomedical and pharmacological studies on genus Sophora, Fabaceae. Revista Brasileira de Farmacognosia, vol.22, p.1145-1154.
9 Matkowski A., Piotrowska M. (2006) Antioxidant and free radical scavenging activities of some medicinal plants from the Lamiaceae. Fitoterapia, no. 77, p. 346-353.
10 Patel A. et al. (2010) Estimation of Flavonoid, Polyphenolic Content and In-vitro Antioxidant Capacity of leaves of Tephrosia purpurea Linn. (Leguminosae). International Journal of Pharma Sciences and Research, vol.1, no. 1, p. 66-77.
11 Re R., Pellegrini N., Proteggente A. et al. (1999) Antioxidant activity applying an improved ABTS radical cation depolarization assay. Free Radical Biology and Medicine, vol. 26, no. 9/10, p. 1231-1237.
12 Sarker S. D., Latif Z., Gray A. I. (2005) Natural Products Isolation. New York City: Human Press, 515 p.
13 Ulubelen A., Öksüz S., Topcu G., Gören A.C., Voelter W. (2001) Antibacterial Diterpenes from the Roots of Salvia b lepharochlaena. Journal of natural products, vol. 64, p. 549-551.
14 Belenovskaya L.M. (1991) Rastitelnye resursy SSSR: Tsvetkovye pasteniya, ikh khimicheskii sostav, ispolzovanie. sem. Hippuridaceae-Lobeliaceae [Plant resources of the USSR: Flowering plants, their chemical composition, use of fam. Hippuridaceae – Lobeliaceae]. Moskva: Nauka, p.72-83.
15 Vinogradova T.A., Gazhev B.N., Vinogradov V.M., Martynov V.K. (1998) Prakticheskaya fitoterapiya [Practical herbal medicine]. Moskva: Olma-Press, SPb.: Neva, Valeri, 640 p.
16 Goloskov V.R. (1969) Illustrirovannyj opredelitel’ rastenij Kazakhstana [Illustrated determinant of plants of Kazakhstan]. Alma-Ata: Nauka, vol. 1, 243 p.
17 Muzychkina RA, Korulkin D.Yu. (2006) Biologicheski aktivnye veshestva rastenii: Vydelenie, razdelenie, analiz [Biologically active substances of plants. Isolation, separation, analysis]. Almaty: Atamura, 438 p.
18 Muzychkina R.A., Korulkin D.Yu., Abilov Zh.A. (2010) Osnovy khimij prirodnykh soedinenij [Fundamentals of the chemistry of natural compounds]. Almaty: Kazakh universitety, 564 p.
19 Pavlov N.V. (1966) Flora Kazakhstana [Flora of The Kazakhstan]. Alma-Ata: Akademiya nauk Kazakhskoi SSR, vol. 9, 654 p.
20 Pavlova L.I., Kaliekova K.S., Ozhmukhametova E.K. (2013) Primenenie lekarstvennykh rastenij, soderzhashikh biologicheskie aktivnye veshestva razlichnoi khimicheskoi struktury [Application of medicinal plants containing biological active substances of different chemical structure]. Nauka i zdravookhranenie, no. 5, p. 67-68.
21 Pat. 2490628 RF. (2013) Sposob opredeleniya soderzhaniya askorbinovoj kisloty [Method for determination of ascorbic acid content]. Е.А. Borodin; publ. 20.08.13, Bul. № 23, 4 p.
22 Stepanova E.F. (1989) Biotekhnologicheskie issledovaniya po bezotkhodnomu ispolzovaniyu solodki [Biotechnological research on waste-free use of licorice]. Abstract of the diss. … d. pharm. s.: 15.00.01. - Kharkov, 1989. - 47 p.
23 Tarlykov P.V., Berdin A.G., Kusainova D.D., Khabarov I.A., Tuleuov B.I. etc. (2006) Rasteniya Kazakhstana – perspektivnye istochniki novykh adaptogennykh preparatov [Plants of Kazakhstan - promising sources of new adaptogenic preparations]. Abstracts of the X International Congress "Fitofarm 2006", SPb: NIIKH SPbGU, p. 321.
24 Cherevatyi V.T. (1980) Sravnitelnoe izuchenie antibakterialnogo deistviya razlichnykh ekstraktov iz shalfeya lekarstvennogo [Comparative study of the antibacterial action of various extracts from the medicinal sage]. Plant resources, is. 16, no. 1, p. 137-139.
25 Chikov P.S. (2002) Lekarstvennye rasteniya [Medicinal plants]. Moskva: Znanie, 496 p.
2 Павлова Л.И., Калиекова К.С., Ожмухаметова Э.К. Применение лекарственных растений, содержащих биологические активные вещества различной химической структуры // Наука и здравоохранение. - 2013. - № 5. - С. 67-68.
3 Biswas N.N., Saha S., Ali M. Kh. Antioxidant, antimicrobial, cytotoxic and analgesic activities of ethanolic extract of Mentha arvensis L. Asian Pacific // Journal of Tropical Biomedicine. - 2014. - № 4(10). - Р. 792-797.
4 Matkowski A., Piotrowska M. Antioxidant and free radical scavenging activities of some medicinal plants from the Lamiaceae // Fitoterapia. - 2006. - № 77. - Р. 346–353.
5 Bettaieb I., Hamrouni-Sellami I., Bourgou S. et al. Drought effects on polyphenol composition and antioxidant activities in aerial parts of Salvia officinalis L. // ActaPhysiol Plant. - 2011. - № 33. - Р. 1103–1111.
6 Genta S.B., Cabrera W.M., Mercado M.I., Grau A., Catalan C.A., Sanchez S.S. Hypoglycemic activity of leaf organic extracts from Smallanthus sonchifolius: Constituents of the most active fractions // Chemico-Biological Interactions. – 2010. - Vol.185. - Р.143–152.
7 Grover J.K, Yadav S.P. Pharmacological actions and potential uses of Momordica charantia: A review // Journal of Ethnopharmacology. - 2004. - Vol. 93. - Р.123–132.
8 Виноградова Т.А., Гажев Б.Н., Виноградов В.М., Мартынов В.К. Практическая фитотерапия. – М.: Олма-Пресс, СПб.: Нева, Валери. - 1998. – 640 с.
9 Тарлыков П.В., Бердин А.Г., Кусаинова Д.Д., Хабаров И.А., Тулеуов Б.И. и др. Растения Казахстана - перспективные источники новых адаптогенных препаратов // Материалы Х Международного съезда «Фитофарм 2006». - СПб: НИИХ СПбГУ, 2006. - С. 321.
10 Музычкина Р.А., Корулькин Д.Ю. Биологически активные вещества растений. Выделение, разделение, анализ. - Алматы: Атамұра, 2006. - 438 с.
11 Череватый В.Т. Сравнительное изучение антибактериального действия различных экстрактов из шалфея лекарственного // Растительные ресурсы. - 1980. - Т. 16. - № 1. - С. 137-139.
12 Grover J.K., Vats V., Rathi S.S. Medicinal plants of India with anti-diabetic potential // Journal of Ethnopharmacology. – 2000. - № 73. - Р. 461–470.
13 Степанова Э. Ф. Биотехнологические исследования по безотходному использованию солодки: автореф. дисс. д. фарм. н.: 15.00.01. - Харьков, 1989. - 47 с.
14 Павлов Н.В. Флора Казахстана - Алма-Ата: Академия наук Казахской ССР. - 1966. - Т.9. - 654 c.
15 Голосков В.Р. Иллюстрированный определитель растений Казахстана. - Алма-Ата: Наука. - 1969. - Т.1. - 243 с.
16 Музычкина Р.А., Корулькин Д.Ю., Абилов Ж.А. Основы химии природных соединений. - Алматы: Қазақ университеті. - 2010. - 564 с.
17 Sarker S. D., Latif Z., Gray A. I. Natural Products Isolation. - New York City: Human Press. -2005. - 515 p.
18 Clinical and Laboratory Standards Institute. Methods for dilution antimicrobial susceptibility test for bacteria that grow aerobically. – 2006, Seventh edition. - Document M7-A7. – 26 p.
19 Re R., Pellegrini N., Proteggente A. et al. Antioxidant activity applying an improved ABTS radical cation depolarization assay // Free Radical Biology and Medicine. - 1999. - Vol. 26. - № 9/10. - P. 1231-1237.
20 Patel A. et al. Estimation of Flavonoid, Polyphenolic Content and In-vitro Antioxidant Capacity of leaves of Tephrosia purpurea Linn. (Leguminosae) // International Journal of Pharma Sciences and Research. - 2010. - Vol.1. - N 1. - P. 66-77.
21 Пат. 2490628 РФ. Способ определения содержания аскорбиновой кислоты / Е.А. Бородин; опубл. 20.08.13, Бюл. № 23. – 4 с.
22 Chang W.T., Huang W.C., Liou C.J. Evaluation of the anti-inflammatory effects of phloretin and phlorizin in lipopolysaccharide-stimulated mouse macrophages // Food Chemistry. - 2012. - Vol.134. - P. 972-979.
23 Беленовская Л. М. Растительные ресурсы СССР: Цветковые растения, их химический состав, использование. cем. Hippuridaceae - Lobeliaceae. - М.: Наука. - 1991. - С.72-83.
24 Krishna P.M., KNV R., Sandhya S., Banji D. A review on phytochemical, ethnomedical and pharmacological studies on genus Sophora, Fabaceae // Revista Brasileira de Farmacognosia. – 2012. – Vol.22. – P.1145-1154.
25 Ulubelen A., Öksüz S., Topcu G., Gören A.C., Voelter W. Antibacterial Diterpenes from the Roots of Salvia b lepharochlaena // Journal of natural products. – 2001. – Vol. 64. – P. 549-551.
References
1 Bettaieb I., Hamrouni-Sellami I., Bourgou S. et al. (2011) Drought effects on the polyphenol composition and antioxidant activities in the aerial parts of Salvia officinalis L. ActaPhysiol Plant., no. 33, p. 1103-1111.
2 Biswas N.N., Saha S., Ali M. Kh. (2014) Antioxidant, antimicrobial, cytotoxic and analgesic activities of ethanolic extract of Mentha arvensis L. Asian Pacific. Journal of Tropical Biomedicine, no. 4 (10), p. 792-797.
3 Chang W.T., Huang W. C., Liou C.J. (2012) Evaluation of the anti-inflammatory effects of phloretin and phlorizin in lipopolysaccharide-stimulated mouse macrophages. Food Chemistry, vol. 134, p. 972-979.
4 Clinical and Laboratory Standards Institute. (2006) Methods for dilution antimicrobial susceptibility. Seventh edition, Document M7-A7, 26 p.
5 Genta S.B., Cabrera W.M., Mercado M. I., Grau A., Catalan C. A., Sanchez S.S. (2010) Hypoglycemic activity of leaf organic extracts from Smallanthus sonchifolius: Constituents of the most active fractions. Chemico-Biological Interactions, vol.185, p. 143-152.
6 Grover J.K, Yadav S.P. (2004) Pharmacological actions and potential uses of Momordica charantia: A review. Journal of Ethnopharmacology, vol. 93, p. 123-132.
7 Grover J.K., Vats V., Rathi S.S. (2000) Medicinal plants of India with anti-diabetic potential. Journal of Ethnopharmacology, no. 73, p. 461-470.
8 Krishna P.M., KNV R., Sandhya S., Banji D. (2012) A review on phytochemical, ethnomedical and pharmacological studies on genus Sophora, Fabaceae. Revista Brasileira de Farmacognosia, vol.22, p.1145-1154.
9 Matkowski A., Piotrowska M. (2006) Antioxidant and free radical scavenging activities of some medicinal plants from the Lamiaceae. Fitoterapia, no. 77, p. 346-353.
10 Patel A. et al. (2010) Estimation of Flavonoid, Polyphenolic Content and In-vitro Antioxidant Capacity of leaves of Tephrosia purpurea Linn. (Leguminosae). International Journal of Pharma Sciences and Research, vol.1, no. 1, p. 66-77.
11 Re R., Pellegrini N., Proteggente A. et al. (1999) Antioxidant activity applying an improved ABTS radical cation depolarization assay. Free Radical Biology and Medicine, vol. 26, no. 9/10, p. 1231-1237.
12 Sarker S. D., Latif Z., Gray A. I. (2005) Natural Products Isolation. New York City: Human Press, 515 p.
13 Ulubelen A., Öksüz S., Topcu G., Gören A.C., Voelter W. (2001) Antibacterial Diterpenes from the Roots of Salvia b lepharochlaena. Journal of natural products, vol. 64, p. 549-551.
14 Belenovskaya L.M. (1991) Rastitelnye resursy SSSR: Tsvetkovye pasteniya, ikh khimicheskii sostav, ispolzovanie. sem. Hippuridaceae-Lobeliaceae [Plant resources of the USSR: Flowering plants, their chemical composition, use of fam. Hippuridaceae – Lobeliaceae]. Moskva: Nauka, p.72-83.
15 Vinogradova T.A., Gazhev B.N., Vinogradov V.M., Martynov V.K. (1998) Prakticheskaya fitoterapiya [Practical herbal medicine]. Moskva: Olma-Press, SPb.: Neva, Valeri, 640 p.
16 Goloskov V.R. (1969) Illustrirovannyj opredelitel’ rastenij Kazakhstana [Illustrated determinant of plants of Kazakhstan]. Alma-Ata: Nauka, vol. 1, 243 p.
17 Muzychkina RA, Korulkin D.Yu. (2006) Biologicheski aktivnye veshestva rastenii: Vydelenie, razdelenie, analiz [Biologically active substances of plants. Isolation, separation, analysis]. Almaty: Atamura, 438 p.
18 Muzychkina R.A., Korulkin D.Yu., Abilov Zh.A. (2010) Osnovy khimij prirodnykh soedinenij [Fundamentals of the chemistry of natural compounds]. Almaty: Kazakh universitety, 564 p.
19 Pavlov N.V. (1966) Flora Kazakhstana [Flora of The Kazakhstan]. Alma-Ata: Akademiya nauk Kazakhskoi SSR, vol. 9, 654 p.
20 Pavlova L.I., Kaliekova K.S., Ozhmukhametova E.K. (2013) Primenenie lekarstvennykh rastenij, soderzhashikh biologicheskie aktivnye veshestva razlichnoi khimicheskoi struktury [Application of medicinal plants containing biological active substances of different chemical structure]. Nauka i zdravookhranenie, no. 5, p. 67-68.
21 Pat. 2490628 RF. (2013) Sposob opredeleniya soderzhaniya askorbinovoj kisloty [Method for determination of ascorbic acid content]. Е.А. Borodin; publ. 20.08.13, Bul. № 23, 4 p.
22 Stepanova E.F. (1989) Biotekhnologicheskie issledovaniya po bezotkhodnomu ispolzovaniyu solodki [Biotechnological research on waste-free use of licorice]. Abstract of the diss. … d. pharm. s.: 15.00.01. - Kharkov, 1989. - 47 p.
23 Tarlykov P.V., Berdin A.G., Kusainova D.D., Khabarov I.A., Tuleuov B.I. etc. (2006) Rasteniya Kazakhstana – perspektivnye istochniki novykh adaptogennykh preparatov [Plants of Kazakhstan - promising sources of new adaptogenic preparations]. Abstracts of the X International Congress "Fitofarm 2006", SPb: NIIKH SPbGU, p. 321.
24 Cherevatyi V.T. (1980) Sravnitelnoe izuchenie antibakterialnogo deistviya razlichnykh ekstraktov iz shalfeya lekarstvennogo [Comparative study of the antibacterial action of various extracts from the medicinal sage]. Plant resources, is. 16, no. 1, p. 137-139.
25 Chikov P.S. (2002) Lekarstvennye rasteniya [Medicinal plants]. Moskva: Znanie, 496 p.
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Kustova, T. S., Samir, A. R., Karpenyuk, T. A., Goncharova, A. V., Favarisova, N. R., Tufuminova, Y. S., & Kenesheva, S. T. (2018). Biological activity of extracts from the roots of Vexibia alopecuroides and Salvia deserta. Experimental Biology, 74(1), 32–41. https://doi.org/10.26577/eb-2018-1-1313
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