Toxic and mutagenic effect of biologically active substances from Inula britannica L. (family Compositae)
76 52
Keywords:
biologically active substances, mutagen, seed germination, chromosome aberrations, Inula britannica.Abstract
Biologically active substances (BAS) from the shoot and root parts of Inula britannica L. (Compositae family) have been obtained. Purity indicators (moisture, total ash); indicators of extractive substances, amino acid and fatty acid compositions have been determined. The main groups of biologically active substances were identified using one- and two dimensional paper chromatography, thin layer chromatography on fixed layer of sorbent. Comparative analysis of the quantitative content of BAS from the shoot and root parts of the Inula britannica showed that the root part is dominated by saponins, tanning substances, polysaccharides, flavonoids, and B2 and C vitamins, and the shoot part is dominated by amino- and organic acids. As a result of the extraction of the shoot and root parts of Inula britannica followed by freeze-drying contingent phytoproducts consisting of BAS were obtained and tested for toxic and mutagenic activity. Toxic and mutagenic effects of BAS from the shoot and root parts of I. britannica have been studied using barley seed germination test and chromosome aberration assay of the germ cells from the root tip of barley. It is found that the BAS in the studied concentrations (25.0, 50.0 and 100.0 mg/l) had no phytotoxic and mutagenic action. The barley seeds treated with aqueous solutions of elecampane extracts at all concentrations had shown no reduction in germination as compared with control plants. Germination of seeds treated with aqueous solutions of methyl methanesulfonate (MMS, classical mutagen) at concentrations of 5.0 mg / L was 74.33%. Comparative analysis showed that the germination of seeds treated with the concentrations of MMS fell 1,26 (p<0.01) times as compared with the control. The frequency of structural chromosome aberrations and the number of chromosomal aberrations per 100 metaphases in root tip cells of barley seeds treated with aqueous solutions of BAS had not significantly differed from the same parameters in untreated seeds. At the same time, the classical mutagen methyl methanesulfonate, used as a positive control, caused significant increase of the studied parameters not only compared with the control, but also the seeds treated with aquatic solutions of the BAS. Thus, extracts from the shoot and root parts of Inula britannica had no toxic and mutagenic activity. Given the high content of biologically active substances in these extracts investigation of antimutagenic potential of I. britannica is a promising study.
References
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22 Zahin M., Aqil F., Ahmad I. Broad spectrum antimutagenic activity of antioxidant active fraction of Punica granatum L. peel extract // Mutat. Res. – 2010. - Vol. 703, No. 2. – Р. 99–107. DOI: 10.1016/j.mrgentox.2010.08.001
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2 Гончарова Р.И., Кужир Т.Д. Молекулярные основы применения антимутагенов в качестве антиканцерогенов // Экологическая генетика. – 2005. – Т. 3, № 3. – С. 19-32.
3 Uzun F., Kalender S., Durak D., Demir F., Kalender Y. Malathion-induced testicular toxicity in male rats and the protective effect of vitamins C and E // Food and Chemical Toxicology. – 2007. – Vol. 47, No. 8. – Р. 1903-1908. DOI: 10.1016/j.fct.2009.05.001.
4 Seca A.M.L., Grigore A., Pinto D.C.G.A., Silva A.M.S. The genus Inula and their metabolites: From ethnopharmacological to medicinal uses // Journal of Ethnopharmacology. – 2014. - Vol. 154, № 2. – P. 286-310. DOI: 10.1016/j.jep.2014.04.010
5 de Souza P., Gasparotto A.Jr., Crestani S., Stefanello M.Е.A., Marques M.C.A., da Silva-Santos J.E., Kassuya C.A.L. Hypotensive mechanism of the extracts and artemetin isolated from Achillea millefolium L. (Asteraceae) in rats // Phytomedicine. - 2011. - Vol. 18. - Р. 819– 825. DOI: 10.1016/j.phymed.2011.02.005
6 Shaikh T., Rub R.A., Sasikumar S. Antimicrobial screening of Cichorium intybus seed extracts // Arabian Journal of Chemistry. - 2012. - DOI: 10.1016/j.arabjc.2012.04.012
7 Fischedick J.T., Pesic M., Podolski-Renic A., Bankovic J., de Vos R.C.H., Perić M., Todorović S., Tanic N. Cytotoxic activity of sesquiterpene lactones from Inula britannica on human cancer cell lines // Phytochemistry Letters. - 2013. - Vol. 6, № 2. - P. 246–252. DOI:10.1016/j.phytol.2013.02.006
8 Radulović N.S., Dekić M.S., Ranđelović P.J., Stojanović N.M., Zarubica A.R., Stojanović-Radić Z.Z. Toxic essential oils: Anxiolytic, antinociceptive and antimicrobial properties of the yarrow Achillea umbellata Sibth. et Sm. (Asteraceae) volatiles // Food and Chemical Toxicology. - 2012. - Vol. 50, № 6. - P. 2016-2026. DOI: 10.1016/j.fct.2012.03.047
9 Liu W., Di Giorgio C., Lamidi M., Elias R., Ollivier E., De Meo M.P. Genotoxic and clastogenic activity of saponins extracted from Nauclea bark as assessed by the micronucleus and the comet assays in Chinese Hamster Ovary cells // Journal of Ethnopharmacology. – 2011. - Vol. 137. – Р. 176 – 183. DOI: 10.1016/j.jep.2011.05.005.
10 Kalantari H., Galehdari H., Zaree Z., Gesztelyi R., Varga B., Haines D., Bombicz M., Tosaki A., Juhasz B. Toxicological and mutagenic analysis of Artemisia dracunculus (tarragon) extract // Food and Chemical Toxicology. – 2013. - Vol. 51. – Р. 26–32. DOI: 10.1016/j.fct.2012.07.052.
11 Saraç N., Şen B. Antioxidant, mutagenic, antimutagenic activities, and phenoliccompounds of Liquidambar orientalis Mill. var. Orientalis // Industrial Crops and Products. – 2014. - Vol. 53. – Р. 60–64. DOI: 10.1016/j.indcrop.2013.12.015
12 Агабейли Р.А. Антимутагенная активность масла плодов Fagus Orientalis (Fagaceae) // Растительные ресурсы. – 2012. – Т. 48, № 2. – С. 267-273.
13 Гераськин С.А., Сарапульцева Е.А. Биологический контроль окружающей среды. Генетический мониторинг. – М.: Академия, 2010. – 208 с. ISBN: 978-5-7695-6536-6.
14 ГОСТ 24027.2-80 Сырье лекарственное растительное. Методы определения влажности, содержания золы, экстрактивных и дубильных веществ, эфирного масла, Лекарственное растительное сырье. Часть 2. Корни, плоды, сырье. – М.: Изд-во стандартов, 1999. - C. 119-126.
15 Государственная фармакопея Республики Казахстан. Т 1. – Алматы: Жибек жолы, 2008. – 592 с.
16 Худолей В.В. Канцерогены: характеристики, закономерности, механизмы действия. – СПб.: НИИ Химии СПбГУ, 1999. – С. 374–375. ISBN: 5-7997-0170-4.
17 Natarajan A.T. Chemical mutagenesis: From plants to human // Current science. – 2005. – Vol. 89, No. 2. – Р. 312-317.
18 Чеснокова С.М. Биологические методы оценки качества объектов окружающей среды. В 2 ч. Ч.2. Методы биотестирования. - Владимир: Изд-во Владим. гос. ун-та, 2008. – 92 с. ISBN: 978-5-89368-829-0.
19 Немцева Л.С. Метафазный метод учета перестроек хромосом. – М.: Наука, 1970. - 126 с.
20 Лакин Г.Ф. Биометрия. - М.: Высшая школа, 1990. - 352 с. - ISBN 5-06-000471-6.
21 Чиркин А.А., Данченко Е.О. Биохимия. – М.: Медицинская литература, 2010. – 624 с. ISBN: 789-5-876965-002-86789.
22 Zahin M., Aqil F., Ahmad I. Broad spectrum antimutagenic activity of antioxidant active fraction of Punica granatum L. peel extract // Mutat. Res. – 2010. - Vol. 703, No. 2. – Р. 99–107. DOI: 10.1016/j.mrgentox.2010.08.001
23 Devasagayam T.P., Tilak J.C., Boloor K.K., Sane K.S., Ghaskadbi S.S., Lele R.D. Free Radicals and Antioxidants in Human Health: Current Status and Future Prospects // J. Assoc. Physicians India. - 2004. - Vol. 52. – Р. 794-804.
24 Абилев С.К., Сартаев А. Избранные лекции по генетике (мутагенез и генотоксикология). – Алматы, 2012. – 208 с. ISBN: 978-601-224-371-0.
25 Santos-Buelga C., Scalbert A. Proantocyanidins and tanninlike compounds: nature, occurrence dietary intake and effects on nutrition and health. // J. Sci. Food Agric. - 2000. - Vol. 80. - P. 1094–1117.
References
1 Druzhinin VG (2003) Quantitative characteristics of chromosome aberration frequency in the human population of a large Western Siberian industrial region, Russian Journal of Genetics, 10 (39): 1161-1167.
2 Goncharova RI, Kuzhir TD (2005) Molecular basis of applying antimutagens as anticarcinogens. Ecological genetics [Molekuliarnye osnovy primeneniia antimutagenov v kachestve antikantserogenov. Ekologicheskaia genetika] 3 (3): 19-32. (In Russian)
3 Uzun F, Kalender S, Durak D, Demir F, Kalender Y (2007) Malathion-induced testicular toxicity in male rats and the protective effect of vitamins C and E, Food and Chemical Toxicology, 47 (8): 1903-1908. DOI: 10.1016/j.fct.2009.05.001.
4 Seca AML, Grigore A, Pinto DCGA, Silva AMS (2014) The genus Inula and their metabolites: From ethnopharmacological to medicinal uses, Journal of Ethnopharmacology, 154(2): 286-310. DOI: 10.1016/j.jep.2014.04.010
5 de Souza P, Gasparotto AJr, Crestani S, Stefanello MЕA, Marques MCA, da Silva-Santos JE, Kassuya CAL (2011) Hypotensive mechanism of the extracts and artemetin isolated from Achillea millefolium L. (Asteraceae) in rats, Phytomedicine, 18: 819– 825. DOI: 10.1016/j.phymed.2011.02.005
6 Shaikh T, Rub RA, Sasikumar S (2012) Antimicrobial screening of Cichorium intybus seed extracts, Arabian Journal of Chemistry. DOI: 10.1016/j.arabjc.2012.04.012
7 Fischedick JT, Pesic M, Podolski-Renic A, Bankovic J, de Vos RCH, Perić M, Todorović S, Tanic N (2013) Cytotoxic activity of sesquiterpene lactones from Inula britannica on human cancer cell lines, Phytochemistry Letters, 6 (2): 246–252. DOI:10.1016/j.phytol.2013.02.006
8 Radulović NS, Dekić MS, Ranđelović PJ, Stojanović NM, Zarubica AR, Stojanović-Radić ZZ (2012) Toxic essential oils: Anxiolytic, antinociceptive and antimicrobial properties of the yarrow Achillea umbellata Sibth. et Sm. (Asteraceae) volatiles, Food and Chemical Toxicology, 50 (6): 2016-2026. DOI: 10.1016/j.fct.2012.03.047
9 Liu W, Di Giorgio C, Lamidi M, Elias R, Ollivier E, De Meo MP (2011) Genotoxic and clastogenic activity of saponins extracted from Nauclea bark as assessed by the micronucleus and the comet assays in Chinese Hamster Ovary cells, Journal of Ethnopharmacology, 137: 176 – 183. DOI: 10.1016/j.jep.2011.05.005.
10 Kalantari H, Galehdari H, Zaree Z, Gesztelyi R, Varga B, Haines D, Bombicz M, Tosaki A, Juhasz B (2013) Toxicological and mutagenic analysis of Artemisia dracunculus (tarragon) extract, Food and Chemical Toxicology, 51: 26–32. DOI: 10.1016/j.fct.2012.07.052.
11 Saraç N, Şen B (2014) Antioxidant, mutagenic, antimutagenic activities, and phenoliccompounds of Liquidambar orientalis Mill. var. Orientalis, Industrial Crops and Products, 53: 60– 64. DOI: 10.1016/j.indcrop.2013.12.015
12 Agabeili RA (2012) Antimutagenic activity of oil fruits Fagus Orientalis (Fagaceae). Plant Resourses [Antimutagennaia aktivnost' masla plodov Fagus Orientalis (Fagaceae). Rastitel'nye resursy]. 2 (48): 267-273. (In Russian)
13 Geras'kin SA, Sarapul'tseva EA (2010) Biological control of environment. Genetic monitoring [Biologicheskii kontrol' okruzhaiushchei sredy. Geneticheskii monitoring]. Akademiia, Моscow, Russia, pp. 208. ISBN: 978-5-7695-6536-6. (In Russian).
14 SS 24027.2-80 Medicinal plant raw. Methods for determination of moisture, ash, extractives and tannins, essential oil, medicinal plant raw materials. Part 2: The roots, fruits, raw. [Syr'e lekarstvennoe rastitel'noe. Metody opredeleniia vlazhnosti, soderzhaniia zoly, ekstraktivnykh i dubil'nykh veshchestv, efirnogo masla, Lekarstvennoe rastitel'noe syr'e. Chast' 2. Korni, plody, syr'e]. Мoscow, Russia, 1999. (In Russian).
15 State Pharmacopoeia of the Republic of Kazakhstan. V. 1 [Gosudarstvennaia farmakopeia Respubliki Kazakhstan. T 1]. Zhibek zholy, Almaty, Kazakhstan, 2008. – 592 р. (In Russian).
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Kolumbayeva, S. Z., Lovinskaya, A. V., Akhtaeva, N. Z., Litvinenko, I. A., Voronova, N., Iliiasova, A. I., & Alikul, A. (2018). Toxic and mutagenic effect of biologically active substances from Inula britannica L. (family Compositae). Experimental Biology, 70(1), 86–97. Retrieved from https://bb.kaznu.kz/index.php/biology/article/view/1255
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МOLECULAR BIOLOGY AND GENETICS
