Influence of low and ultralow temperatures on seed laboratory germination of three natural populations of Lonicera iliensis Pojark.
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Keywords:
Lonicera iliensis Pojark., Ili honeysuckle, laboratory germination, seed collection, cryopreservation.Abstract
This article addresses the issue of biodiversity conservation of rare endemic species of Ile-Balkhash region Lonicera iliensis Pojark. by creating seed collections. Seeds were collected from three natural populations of Lonicera iliensis in the Almaty region. It was shown that the viability of the honeysuckle seeds significantly depends on the storage temperature. Lonicera iliensis seed storage for 3 years in uncontrolled conditions (at room temperature) resulted in loss of viability, whereas at + 4°C laboratory germination remained at 49.0-78.3% of initial germination. It was found that the seed stratification after storage at low and ultra-low temperatures (-18°C ...-20°C and -196°C) have a positive effect on their germination. Laboratory germination of honeysuckle Ili seeds stored for three years at 18°C ...-20°C and then held 1-week stratification was 63.3-86.1% of the initial. Laboratory germination of seeds after deep-freezing combined with 1 week of stratification remained at 87.4-100% of control seeds germination. The collections of Lonicera iliensis seeds from three natural populations were established at various storage conditions (+4°C, -18°C ...-20°C and -196°C ), which can be used to restore this species in nature, as well as for the exchange of genetic material.
References
Литература
1. Пояркова А.И. Род Lonicera L. Флора СССР. – М.: Л.: Изд-во АН СССР. – 1958. – Т. 23. – С. 467-573.
2. Флора Казахстана. – А.: Наука. – 1965. – Т. 8. – 448 с.
3. Қазақстанның Қызыл Кітабы. 2-басылым. Өсімдіктер. – Астана:. АртPrintXXI. – 2014. – T. 2. – 452 б.
4. Куклина А.Г., Скворцов А.К. К интродукции жимолости илийской // Бюллетень Главного Ботанического Сада. – 1990. – Вып. 157. – С. 3-9.
5. Регель Э.Л. Русская денрология. СПб. – 1873. Вып. 3.
6. Аметов А.А., Мухитдинов Н.М., Абидкулова К.Т., Карашолакова Л.Н. Характеристика растительных сообществ популяции Lonicera iliensis Pojark. в верхнем течении реки Чилик Алматинской области // Вестник КазНУ, серия экологическая. – 2012. – № 4(36). – С. 44-52.
7. Аметов А.А., Мухитдинов Н.М., Абидкулова К.Т. Современное состояние популяции Lonicera iliensis в среднем течении реки Иле // Материалы конференции «Сохранение степных и полупустынных экосистем Евразии». Алматы. – 2013. – С. 68.
8. Тихонова Н.Г., Филипенко Г.И., Вержук В.Г., Жестков А.С. Стратегия и методы длительного хранения генофонда растений // Проблемы криобиологии. – 2008. – № 18(2). – С. 227.
9. Новикова Т.И. Использование биотехнологических подходов для сохранения биоразнообразия растений // Растительный мир Азиатской России – 2013. – № 2(12). – С. 119-128.
10. Cruz-Cruz C.A., Gonzalez-Arnao M.T., Engelmann F. Biotechnology and conservation of plant biodiversity // Resources. – 2013. – № 2. – P. 73-95.
11. Тихонова В.Л,. Шугаева Е.В., Фирсанова В.М. Жизнеспособность семян некоторых видов дикорастущих лекарственных растений при глубоком и неглубоком замораживании // Растительные ресурсы. – 1996. – № 32(3). – С. 43-50.
12. Тихонова В.Л. Долговременное хранение семян // Физиология растений. – 1999. – № 46(3). –С. 467-476.
13. Reed B.M. Plant Cryopreservation. A Practical Guide. – LLC: Springer Science Business Media, – 2008. – 532 p.
14. Kushnarenko S., Salnikov E., Nurtazin M., Mukhitdinova Z., Rakhimbaev I., Reed B.M. Characterization and cryopreservation of Malus sieversii seeds // The Asian and Australian Journal Plant Science Biotechnology. – 2010. – № 4(1). – С. 5-9.
15. Семена деревьев и кустарников. Методы определения влажности = Seeds of trees and shrubs. Methods for determination of moisture: государственный стандарт Союза ССР ГОСТ 13056.3-86: – Москва. – 1986. – 59 с.
16. Лакин Г.Ф. Биометрия. – М.: Высшая школа. – 1990. – 352 с.
17. Bonner F.T., Karrafalt R.P. Storage of Seeds. The Woody Plant Seed Manual. – Missisipi. – 2008. – P. 682-688.
18. Николаева М.Г., Разумова М.В., Гладкова В.Н. Справочник по проращиванию покоящихся семян, – Л.: Изд-во Наука. – 1985. – 343 с.
19. Романюк В.В. Особенности прорастания семян жимолости. Экологические проблемы семеноводства интродуцентов // Тезисы докладов VII всесоюз. конф., Рига. – 1984. – С. 107-108.
20. Сорокин А.А. Совершенствование приемов семенного и вегетативного размножения жимолости синей. Автореферат, Санкт-Петербург, 2002. – 15 c.
21. Brinkman, K.A., Lonicera L. Honeysuckle. Seeds Of Woody Plants In The United States. Agriculture Handbook 450. – Washington: U. S. Department of Agriculture. Forest Service, – 1974. – P. 515-519.
22. Холина А.Б., Воронкова Н.М. Сохранение генофонда дальневосточных растений методом криоконсервации семян // Известия РАН, Серия биологическая. – 2008. – № 3. – С. 304–312.
References
1. Pojarkova AI (1958) The genus Lonicera L. Flora of the USSR, Publishing House of the USSR Academy of Sciences [Rod Lonicera L. Flora SSSR, Izd-vo AN SSSR] 23:467-573. (In Russian)
2. Flora of Kazakhstan (1965) Science [Flora Kazahstana. Nauka] 8:448. (In Russian)
3. Red Book of Kazakhstan (2014) second edition. Plants. АртPrintXXI. – 2014. – 2:452.
4. Kuklina AG, Skvorcov AK (1990) Introduction of Ili honeysuckle. Bulletin of the Main Botanical Garden [K introdukcii zhimolosti ilijskoj. Byulleten Glavnogo Botanicheskogo Sada] 157:3-9. (In Russian)
5. Regel EL (1873) Russian dendrology [Russkaya dendrologiya] SPb. 3.
6. Ametov AA, Mukhitdinov NM, Abidkulova КT, Karasholakova LN (2012) Characterization of plant community population Lonicera iliensis Pojark. in the upper reaches of the river Chilik Almaty region. Bulletin of KazNU, series ecological [Kharakteristika rastitel'nykh soobshchestv populyatsii Lonicera iliensis Pojark. v verkhnem techenii reki Chilik Almatinskoy oblasti. Vestnik KazNU, seriya ekologicheskaya] 4(36): 44-52. (In Russian)
7. Ametov AA, Mukhitdinov NM, Abidkulova KT (2013) The current state of Lonicera iliensis population in the middle reaches of the Ili River. Saving the steppe and semi-desert ecosystems of Eurasia, Almaty [Sovremennoye sostoyaniye populyatsii Lonicera iliensis v srednem techenii reki Ile. Sokhraneniye stepnykh i polupustynnykh ekosistem Yevrazii] P. 68. (In Russian)
8. Tikhonova NG, Filipenko GI, Verzhuk VG, Zhestkov AS (2008) Strategy and methods of long-term storage of gene fond of plants. Problems of Cryobiology. 18(2):227.
9. Novikova TI (2013) The use of biotechnological approaches for the conservation of plant diversity. The flora of the Asian part of Russia, [Ispol'zovaniye biotekhnologicheskikh podkhodov dlya sokhraneniya bioraznoobraziya rasteniy. Rastitel'nyy mir Aziatskoy Rossiii,] 2(12):119-128. (In Russian)
10. Cruz-Cruz CA, Gonzalez-Arnao MT, Engelmann F (2013) Biotechnology and conservation of plant biodiversity. Resources. 2:73-95.
11. Tikhonova VL, Shugayeva YEV, Firsanova VM (1996) The viability of the seeds of some species of wild medicinal plants with deep and shallow freezing. Plant resources [Zhiznesposobnost' semyan nekotorykh vidov dikorastushchikh lekarstvennykh rasteniy pri glubokom i neglubokom zamorazhivanii. Rastitel'nyye resursy] 32(3):43-50. (In Russian)
12. Tikhonova VL (1999) Long-term storage of seeds. Plant Physiology [Dolgovremennoye khraneniye semyan. Fiziologiya rasteniy] 46(3):467-476.
13. Reed BM (2008) Plant Cryopreservation. A Practical Guide. LLC: Springer Science Business Media, – 532 p.
14. Kushnarenko S, Salnikov E, Nurtazin M, Mukhitdinova Z, Rakhimbaev I, Reed BM (2010) Characterization and cryopreservation of Malus sieversii seeds. The Asian and Australian Journal Plant Science Biotechnology. 4(1):5-9.
15. Seeds of trees and shrubs. Methods for determination of moisture content [Semena derev'yev i kustarnikov. Metody opredeleniya vlazhnosti]: Union State Standard USSR GOST 13056.3-86: Moscow. 1986. (In Russian)
16. Lakin GF (1990) Biometrics. Higher School [Biometrija. Vyisshaya shkola] – 352 р. (In Russian)
17. Bonner FT, Karrafalt RP (2008) Storage of Seeds. The Woody Plant Seed Manual. Missisipi. – P. 682-688.
18. Nikolaeva MG, Razumov MV, Gladkova (1985) The Handbook on germination of dormant seeds. Publishing House of Sciences [Spravochnik po prorashchivaniyu pokoyashchikhsya semyan. Izd-vo Nauka,] – 343 р. (In Russian)
19. Romanyuk VV (1984) Features honeysuckle seed germination. Environmental concerns seed of exotic species: Abstracts of the VII All-Union. Conf., Riga [Osobennosti prorastaniya semyan zhimolosti. Ekologicheskiye problemy semenovodstva introdutsentov: Tezisy dokladov VII vsesoyuz. konf.] – Р. 107-108. (In Russian)
20. Sorokin A.A. (2002) Improving methods of seed and vegetative reproduction of the blue honeysuckle: abstract, St. Petersburg [Sovershenstvovaniye priyemov semennogo i vegetativnogo razmnozheniya zhimolosti siney: avtoreferat, Sankt-Peterburg] – 15 p.
21. Brinkman, KA (1974) Lonicera L. Honeysuckle. Seeds Of Woody Plants In The United States. Agriculture Handbook 450. U. S. Department of Agriculture, – P. 515-519.
22. Cholina AB, Voronkova NM (2008) Preservation of the gene pool of the Far Eastern plants by cryopreservation of seeds. Proceedings of RAS, Biological series [Sokhraneniye genofonda dal'nevostochnykh rasteniy metodom kriokonservatsii semyan. Izvestiya RAN, Seriya biologicheskaya] 3:304-312. (In Russian)
1. Пояркова А.И. Род Lonicera L. Флора СССР. – М.: Л.: Изд-во АН СССР. – 1958. – Т. 23. – С. 467-573.
2. Флора Казахстана. – А.: Наука. – 1965. – Т. 8. – 448 с.
3. Қазақстанның Қызыл Кітабы. 2-басылым. Өсімдіктер. – Астана:. АртPrintXXI. – 2014. – T. 2. – 452 б.
4. Куклина А.Г., Скворцов А.К. К интродукции жимолости илийской // Бюллетень Главного Ботанического Сада. – 1990. – Вып. 157. – С. 3-9.
5. Регель Э.Л. Русская денрология. СПб. – 1873. Вып. 3.
6. Аметов А.А., Мухитдинов Н.М., Абидкулова К.Т., Карашолакова Л.Н. Характеристика растительных сообществ популяции Lonicera iliensis Pojark. в верхнем течении реки Чилик Алматинской области // Вестник КазНУ, серия экологическая. – 2012. – № 4(36). – С. 44-52.
7. Аметов А.А., Мухитдинов Н.М., Абидкулова К.Т. Современное состояние популяции Lonicera iliensis в среднем течении реки Иле // Материалы конференции «Сохранение степных и полупустынных экосистем Евразии». Алматы. – 2013. – С. 68.
8. Тихонова Н.Г., Филипенко Г.И., Вержук В.Г., Жестков А.С. Стратегия и методы длительного хранения генофонда растений // Проблемы криобиологии. – 2008. – № 18(2). – С. 227.
9. Новикова Т.И. Использование биотехнологических подходов для сохранения биоразнообразия растений // Растительный мир Азиатской России – 2013. – № 2(12). – С. 119-128.
10. Cruz-Cruz C.A., Gonzalez-Arnao M.T., Engelmann F. Biotechnology and conservation of plant biodiversity // Resources. – 2013. – № 2. – P. 73-95.
11. Тихонова В.Л,. Шугаева Е.В., Фирсанова В.М. Жизнеспособность семян некоторых видов дикорастущих лекарственных растений при глубоком и неглубоком замораживании // Растительные ресурсы. – 1996. – № 32(3). – С. 43-50.
12. Тихонова В.Л. Долговременное хранение семян // Физиология растений. – 1999. – № 46(3). –С. 467-476.
13. Reed B.M. Plant Cryopreservation. A Practical Guide. – LLC: Springer Science Business Media, – 2008. – 532 p.
14. Kushnarenko S., Salnikov E., Nurtazin M., Mukhitdinova Z., Rakhimbaev I., Reed B.M. Characterization and cryopreservation of Malus sieversii seeds // The Asian and Australian Journal Plant Science Biotechnology. – 2010. – № 4(1). – С. 5-9.
15. Семена деревьев и кустарников. Методы определения влажности = Seeds of trees and shrubs. Methods for determination of moisture: государственный стандарт Союза ССР ГОСТ 13056.3-86: – Москва. – 1986. – 59 с.
16. Лакин Г.Ф. Биометрия. – М.: Высшая школа. – 1990. – 352 с.
17. Bonner F.T., Karrafalt R.P. Storage of Seeds. The Woody Plant Seed Manual. – Missisipi. – 2008. – P. 682-688.
18. Николаева М.Г., Разумова М.В., Гладкова В.Н. Справочник по проращиванию покоящихся семян, – Л.: Изд-во Наука. – 1985. – 343 с.
19. Романюк В.В. Особенности прорастания семян жимолости. Экологические проблемы семеноводства интродуцентов // Тезисы докладов VII всесоюз. конф., Рига. – 1984. – С. 107-108.
20. Сорокин А.А. Совершенствование приемов семенного и вегетативного размножения жимолости синей. Автореферат, Санкт-Петербург, 2002. – 15 c.
21. Brinkman, K.A., Lonicera L. Honeysuckle. Seeds Of Woody Plants In The United States. Agriculture Handbook 450. – Washington: U. S. Department of Agriculture. Forest Service, – 1974. – P. 515-519.
22. Холина А.Б., Воронкова Н.М. Сохранение генофонда дальневосточных растений методом криоконсервации семян // Известия РАН, Серия биологическая. – 2008. – № 3. – С. 304–312.
References
1. Pojarkova AI (1958) The genus Lonicera L. Flora of the USSR, Publishing House of the USSR Academy of Sciences [Rod Lonicera L. Flora SSSR, Izd-vo AN SSSR] 23:467-573. (In Russian)
2. Flora of Kazakhstan (1965) Science [Flora Kazahstana. Nauka] 8:448. (In Russian)
3. Red Book of Kazakhstan (2014) second edition. Plants. АртPrintXXI. – 2014. – 2:452.
4. Kuklina AG, Skvorcov AK (1990) Introduction of Ili honeysuckle. Bulletin of the Main Botanical Garden [K introdukcii zhimolosti ilijskoj. Byulleten Glavnogo Botanicheskogo Sada] 157:3-9. (In Russian)
5. Regel EL (1873) Russian dendrology [Russkaya dendrologiya] SPb. 3.
6. Ametov AA, Mukhitdinov NM, Abidkulova КT, Karasholakova LN (2012) Characterization of plant community population Lonicera iliensis Pojark. in the upper reaches of the river Chilik Almaty region. Bulletin of KazNU, series ecological [Kharakteristika rastitel'nykh soobshchestv populyatsii Lonicera iliensis Pojark. v verkhnem techenii reki Chilik Almatinskoy oblasti. Vestnik KazNU, seriya ekologicheskaya] 4(36): 44-52. (In Russian)
7. Ametov AA, Mukhitdinov NM, Abidkulova KT (2013) The current state of Lonicera iliensis population in the middle reaches of the Ili River. Saving the steppe and semi-desert ecosystems of Eurasia, Almaty [Sovremennoye sostoyaniye populyatsii Lonicera iliensis v srednem techenii reki Ile. Sokhraneniye stepnykh i polupustynnykh ekosistem Yevrazii] P. 68. (In Russian)
8. Tikhonova NG, Filipenko GI, Verzhuk VG, Zhestkov AS (2008) Strategy and methods of long-term storage of gene fond of plants. Problems of Cryobiology. 18(2):227.
9. Novikova TI (2013) The use of biotechnological approaches for the conservation of plant diversity. The flora of the Asian part of Russia, [Ispol'zovaniye biotekhnologicheskikh podkhodov dlya sokhraneniya bioraznoobraziya rasteniy. Rastitel'nyy mir Aziatskoy Rossiii,] 2(12):119-128. (In Russian)
10. Cruz-Cruz CA, Gonzalez-Arnao MT, Engelmann F (2013) Biotechnology and conservation of plant biodiversity. Resources. 2:73-95.
11. Tikhonova VL, Shugayeva YEV, Firsanova VM (1996) The viability of the seeds of some species of wild medicinal plants with deep and shallow freezing. Plant resources [Zhiznesposobnost' semyan nekotorykh vidov dikorastushchikh lekarstvennykh rasteniy pri glubokom i neglubokom zamorazhivanii. Rastitel'nyye resursy] 32(3):43-50. (In Russian)
12. Tikhonova VL (1999) Long-term storage of seeds. Plant Physiology [Dolgovremennoye khraneniye semyan. Fiziologiya rasteniy] 46(3):467-476.
13. Reed BM (2008) Plant Cryopreservation. A Practical Guide. LLC: Springer Science Business Media, – 532 p.
14. Kushnarenko S, Salnikov E, Nurtazin M, Mukhitdinova Z, Rakhimbaev I, Reed BM (2010) Characterization and cryopreservation of Malus sieversii seeds. The Asian and Australian Journal Plant Science Biotechnology. 4(1):5-9.
15. Seeds of trees and shrubs. Methods for determination of moisture content [Semena derev'yev i kustarnikov. Metody opredeleniya vlazhnosti]: Union State Standard USSR GOST 13056.3-86: Moscow. 1986. (In Russian)
16. Lakin GF (1990) Biometrics. Higher School [Biometrija. Vyisshaya shkola] – 352 р. (In Russian)
17. Bonner FT, Karrafalt RP (2008) Storage of Seeds. The Woody Plant Seed Manual. Missisipi. – P. 682-688.
18. Nikolaeva MG, Razumov MV, Gladkova (1985) The Handbook on germination of dormant seeds. Publishing House of Sciences [Spravochnik po prorashchivaniyu pokoyashchikhsya semyan. Izd-vo Nauka,] – 343 р. (In Russian)
19. Romanyuk VV (1984) Features honeysuckle seed germination. Environmental concerns seed of exotic species: Abstracts of the VII All-Union. Conf., Riga [Osobennosti prorastaniya semyan zhimolosti. Ekologicheskiye problemy semenovodstva introdutsentov: Tezisy dokladov VII vsesoyuz. konf.] – Р. 107-108. (In Russian)
20. Sorokin A.A. (2002) Improving methods of seed and vegetative reproduction of the blue honeysuckle: abstract, St. Petersburg [Sovershenstvovaniye priyemov semennogo i vegetativnogo razmnozheniya zhimolosti siney: avtoreferat, Sankt-Peterburg] – 15 p.
21. Brinkman, KA (1974) Lonicera L. Honeysuckle. Seeds Of Woody Plants In The United States. Agriculture Handbook 450. U. S. Department of Agriculture, – P. 515-519.
22. Cholina AB, Voronkova NM (2008) Preservation of the gene pool of the Far Eastern plants by cryopreservation of seeds. Proceedings of RAS, Biological series [Sokhraneniye genofonda dal'nevostochnykh rasteniy metodom kriokonservatsii semyan. Izvestiya RAN, Seriya biologicheskaya] 3:304-312. (In Russian)
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Karasholakova, L. N., & Kushnarenko, S. V. (2016). Influence of low and ultralow temperatures on seed laboratory germination of three natural populations of Lonicera iliensis Pojark. Experimental Biology, 68(3), 54–61. Retrieved from https://bb.kaznu.kz/index.php/biology/article/view/1202
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