Optimization of culture conditions for strains of the genus Streptomyces formulating biopreparation for plant cultivation
101 43
Keywords:
streptomycete, antifungal activity, growth-stimulating activity, cereal crops,Abstract
Biomass growth and biosynthesis of biologically active substances in the strains Streptomyces candidus IMV 37 and Streptomyces canofumeus IMV 541 formulating a preparation for plant cultivation have been studied in 10 organic and synthetic culture media. Considerable effect of medium composition on the growth of Streptomyces strains and formation rate of biologically active substances was shown. The optimal biomass growth of studied Streptomyces strains provide organic medium with soy flour. Medium No. 8 with oat flour is the most optimal for the synthesis of both antifungal antibiotic, and plant growth-stimulating substances. Medium No. 8 supports high-level biomass accumulation (45.8 g/L) of the strain Streptomyces canofumeus IMV 541, and strong antifungal activity of the culture fluid (zone diameter of growth inhibition of the test organisms is within 31-32 mm) and biomass extracts (36 mm). Culture fluid of the strain Streptomyces candidus IMV 37 obtained in the medium No. 8 increases the germination energy of wheat seeds by 26.6%, laboratory germination capacity by 20.0%, length of sprouts 2.2 times, root length 2.0 times, and plant wet weight 1.9 times.
References
Литературa
1 Joo G.J. Production of an antifungal substance for biological control of Phytophthora capsici causing phytophthora blight in red-peppers by Streptomyces halstedii // Biotechnol. Lett. – 2005. – Vol. 27. – P. 201–205.
2 Aryantha P.G., Guest I.D. Mycoparasitic and antagonistic inhibition on Phytophtora cinnamomi Rands by microbial agents isolated from manure composts // Plant Pathology. – 2006. – Vol. 5. – P. 291-298.
3 Propagdee B., Kuekulvong C., Mongkolsuk S. Antifungal potential of extracellular metabolites producer by Streptomyces hygroscopicus againts phytopathogenic fungi // Int. J. Biol. Sci. – 2008. – Vol. 4. – P. 330-337.
4 Дегтярева Е.А., Виноградова К.А., Александрова А.В., Филоненко В.А., Кожевин П.А. Почвенные актиномицеты как потенциальные биофунгициды // Вестник Московского университета, сер. почвоведение. - 2009. - № 2. - С. 22-26.
5 Snehal J., Shinde S.K., Prashanthi P.U., Krishnaraj J. Identification and utilization of actinobacteria for biocontrol of rice sheath blight pathogen Rhizoctonia solani // Asian Journal of Bio Science. – 2014. – Vol. 9, N 2. – P. 227-233.
6 Bouras N., Meklat A., Toumatia O., Mokrane S., Holtz M.D., Strelkov S.E., Sabaou N. Bioactive potential of a new strain of Streptomyces sp. PP14 isolated from Canadian soil // Afr. J. Microbiol. Res. – 2013. - Vol. 7. – N 25. – P. 3199-3208.
7 Широких И.Г. и др. Эффекты интродукции Streptomices hygroscopicus А4 в фитосферу голозерного овса // Зерновое хозяйство России. - 2013. - № 3. - С. 52-56.
8 Brown M.E. Plant growth substances produced by microorganisms of soil and rhizosphere // Journal of Applied Bacteriology. – 1972. – Vol. 35. – P. 443-451.
9 Martens D.A., Frankenberger W.T. Assimilation of exogenous 2'-14C-indole acetic acid and 3'-14C-tryptophan exposed to the roots of three wheat varieties // Plant and Soil. – 1994. – Vol. 166. – P. 281-290.
10 Tokala R.K., Strap J.L., Jung C.M., Crawford D.L., Salove M.H., Deobald L.A., Bailey J.F., Morra M.J. Novel Plant-Microbe Rhizosphere Interaction Involving Streptomyces lydicus WYEC108 nd the Pea Plant (Pisum sativum) // Applied and Environmental Microbiology. – 2002. – Vol. 68. – P. 2161-2171.
11 Waksman S.A. Classification, Identification and Description of Genera and Species. The Actinomycetes. - Baltimore: Waverly Press, 1961. – Vol. 2. – 962 p.
12 Ilić S., Konstantinović S., Veljković B., Savić S., Gojgić-Cvijović G. The impact of different carbon and nitrogen sources on antibiotic production by Streptomyces hygroscopicus CH-7 // Current Research, Technology and Education Topics in Applied Microbiology and Microbial Biotechnology. - 2010. – Vol. 2. – P. 1337-1342.
13 Reddy N.G., Ramakrishna D., Rajagopal S. Optimization of culture conditions of Streptomyces rochei (MTCC 10109) for the production of antimicrobial metabolites // Egyptian Journal of Biology. – 2011. – Vol. 13. – P. 21-29.
14 Егоров Н.С. Основы учения об антибиотиках. - М.: МГУ, Наука, 2004. - 528 с.
15 Locci R. Streptomyces and Related Genera. Bergey’s Manual of Systematic Bacteriology.
- Baltimore: Williams Co, 1989. - Vol. 4. – P. 2451–2508.
16 Salha H.M., Al-Zahrani. Studies on the Antimicrobial Activity of Streptomyces // J. KAU Sci. – 2007. - Vol. 19. – P. 127-138.
17 Gupte M.D., Kulkarni P.R. A study of antifungal antibiotic production by Streptomyces chattanougensis MTCC 3423 using full factorial design // Lett. Appld. Microbiol. – 2002. – Vol. 35, № 1. – P. 22-26.
18 Vieira M.F., Sousa Q., Lopes C.E., Pereira N. J. Production of Actinomycin D by Streptomyces parvulus // Braz. Arch. Biol. Technol. - 2001. – Vol. 44, № 3. – P. 14-22.
19 ГОСТ 12038-84. Семена сельскохозяйственных культур. Методы определения всхожести. Изменение №2 к ГОСТ 12038-84 от 01.06.1995.
20 Урбах В.Ю. Статистический анализ в биологических и медицинских исследованиях. - М.: Медицина, 1975. - 295 с.
References
1 Joo GJ (2005) Production of an antifungal substance for biological control of Phytophthora capsici causing phytophthora blight in red-peppers by Streptomyces halstedii. Biotechnol Lett, 27:201–205.
2 Aryantha PG, Guest ID (2006) Mycoparasitic and antagonistic inhibition on Phytophtora cinnamomi Rands by microbial agents isolated from manure composts. Plant Pathology, 5:291-298.
3 Propagdee B, Kuekulvong C, Mongkolsuk S (2008) Antifungal potential of extracellular metabolites producer by Streptomyces hygroscopicus againts phytopathogenic fungi. Int J Biol Sci, 4:330-337.
4 Degtjareva EA, Vinogradova KA, Aleksandrova AV, Filonenko VA, Kozhevin PA (2009) Soil actinomycetes as potential fungicides. News of Moscow University, ser. soil science [Pochvennye aktinomicety kak potencial'nye biofungicidy. Vestnik Moskovskogo universiteta, ser. pochvovedenie] 2:22-26. (In Russian)
5 Snehal J, Shinde SK, Prashanthi PU, Krishnaraj J (2014) Identification and utilization of actinobacteria for biocontrol of rice sheath blight pathogen Rhizoctonia solani. Asian Journal of Bio Science, 9:227-233.
6 Bouras N, Meklat A, Toumatia O, Mokrane S, Holtz MD, Strelkov SE, Sabaou N (2013) Bioactive potential of a new strain of Streptomyces sp. PP14 isolated from Canadian soil. Afr J Microbiol Res, 7:3199-3208.
7 Shirokih IG e a (2013) The effects of the introduction of Streptomyces hygroscopicus A4 in phytosphere naked oats. Grain farm in Russia [Jeffekty introdukcii Streptomices hygroscopicus A4 v fitosferu golozernogo ovsa. Zernovoe hozjajstvo Rossii] 3:52-56. (In Russian)
8 Brown ME (1972) Plant growth substances produced by microorganisms of soil and rhizosphere. Journal of Applied Bacteriology, 35:443-451.
9 Martens DA, Frankenberger WT (1994) Assimilation of exogenous 2'-14C-indole acetic acid and 3'-14C-tryptophan exposed to the roots of three wheat varieties. Plant and Soil, 166:281-290.
10 Tokala RK, Strap JL, Jung CM, Crawford DL, Salove MH, Deobald LA, Bailey JF, Morra MJ (2002) Novel Plant-Microbe Rhizosphere Interaction Involving Streptomyces lydicus WYEC108 nd the Pea Plant (Pisum sativum). Applied and Environmental Microbiology, 68:2161-2171.
11 Waksman SA (1961) Classification, Identification and Description of Genera and Species. The Actinomycetes. Baltimore, Waverly Press.
12 Ilić S, Konstantinović S, Veljković B, Savić S, Gojgić-Cvijović G (2010) The impact of different carbon and nitrogen sources on antibiotic production by Streptomyces hygroscopicus CH-7. Current Research, Technology and Education Topics in Applied Microbiology and Microbial Biotechnology, 2:1337-1342.
13 Reddy NG, Ramakrishna D, Rajagopal S (2011) Optimization of culture conditions of Streptomyces rochei (MTCC 10109) for the production of antimicrobial metabolites. Egyptian Journal of Biology, 13:21-29.
14 Egorov NS (2004) Fundamentals of theory of antibiotics [Osnovy uchenija ob antibiotikah]. Moscow State University, Moscow, Russia (In Russian)
15 Locci R (1989) Streptomyces and Related Genera. Bergey’s Manual of Systematic Bacteriology. Baltimore: Williams Co, 4:2451–2508.
16 Salha HM, Al-Zahrani (2007) Studies on the Antimicrobial Activity of Streptomyces. J KAU Sci, 19:127-138.
17 Gupte MD, Kulkarni PR (2002) A study of antifungal antibiotic production by Streptomyces chattanougensis MTCC 3423 using full factorial design. Lett Appld Microbiol, 35:22-26.
18 Vieira MF, Sousa Q, Lopes CE, Pereira NJ (2001) Production of Actinomycin D by Streptomyces parvulus. Braz Arch Biol Technol, 44:14-22.
19 GOST 12038-84. Agricultural seeds. Methods for determining the germination. Change №2 to GOST 12038-84 from 01.06.1995 [GOST 12038-84. Semena sel'skohozjajstvennyh kul'tur. Metody opredelenija vshozhesti. Izmenenie #2 k GOST 12038-84 ot 01.06.1995] (In Russian)
20 Urbach VY (1975) Statistical analysis in biological and medical research [Statisticheskij analiz v biologicheskih i medicinskih issledovanijah]. Medicine, Moscow, Russia (In Russian)
1 Joo G.J. Production of an antifungal substance for biological control of Phytophthora capsici causing phytophthora blight in red-peppers by Streptomyces halstedii // Biotechnol. Lett. – 2005. – Vol. 27. – P. 201–205.
2 Aryantha P.G., Guest I.D. Mycoparasitic and antagonistic inhibition on Phytophtora cinnamomi Rands by microbial agents isolated from manure composts // Plant Pathology. – 2006. – Vol. 5. – P. 291-298.
3 Propagdee B., Kuekulvong C., Mongkolsuk S. Antifungal potential of extracellular metabolites producer by Streptomyces hygroscopicus againts phytopathogenic fungi // Int. J. Biol. Sci. – 2008. – Vol. 4. – P. 330-337.
4 Дегтярева Е.А., Виноградова К.А., Александрова А.В., Филоненко В.А., Кожевин П.А. Почвенные актиномицеты как потенциальные биофунгициды // Вестник Московского университета, сер. почвоведение. - 2009. - № 2. - С. 22-26.
5 Snehal J., Shinde S.K., Prashanthi P.U., Krishnaraj J. Identification and utilization of actinobacteria for biocontrol of rice sheath blight pathogen Rhizoctonia solani // Asian Journal of Bio Science. – 2014. – Vol. 9, N 2. – P. 227-233.
6 Bouras N., Meklat A., Toumatia O., Mokrane S., Holtz M.D., Strelkov S.E., Sabaou N. Bioactive potential of a new strain of Streptomyces sp. PP14 isolated from Canadian soil // Afr. J. Microbiol. Res. – 2013. - Vol. 7. – N 25. – P. 3199-3208.
7 Широких И.Г. и др. Эффекты интродукции Streptomices hygroscopicus А4 в фитосферу голозерного овса // Зерновое хозяйство России. - 2013. - № 3. - С. 52-56.
8 Brown M.E. Plant growth substances produced by microorganisms of soil and rhizosphere // Journal of Applied Bacteriology. – 1972. – Vol. 35. – P. 443-451.
9 Martens D.A., Frankenberger W.T. Assimilation of exogenous 2'-14C-indole acetic acid and 3'-14C-tryptophan exposed to the roots of three wheat varieties // Plant and Soil. – 1994. – Vol. 166. – P. 281-290.
10 Tokala R.K., Strap J.L., Jung C.M., Crawford D.L., Salove M.H., Deobald L.A., Bailey J.F., Morra M.J. Novel Plant-Microbe Rhizosphere Interaction Involving Streptomyces lydicus WYEC108 nd the Pea Plant (Pisum sativum) // Applied and Environmental Microbiology. – 2002. – Vol. 68. – P. 2161-2171.
11 Waksman S.A. Classification, Identification and Description of Genera and Species. The Actinomycetes. - Baltimore: Waverly Press, 1961. – Vol. 2. – 962 p.
12 Ilić S., Konstantinović S., Veljković B., Savić S., Gojgić-Cvijović G. The impact of different carbon and nitrogen sources on antibiotic production by Streptomyces hygroscopicus CH-7 // Current Research, Technology and Education Topics in Applied Microbiology and Microbial Biotechnology. - 2010. – Vol. 2. – P. 1337-1342.
13 Reddy N.G., Ramakrishna D., Rajagopal S. Optimization of culture conditions of Streptomyces rochei (MTCC 10109) for the production of antimicrobial metabolites // Egyptian Journal of Biology. – 2011. – Vol. 13. – P. 21-29.
14 Егоров Н.С. Основы учения об антибиотиках. - М.: МГУ, Наука, 2004. - 528 с.
15 Locci R. Streptomyces and Related Genera. Bergey’s Manual of Systematic Bacteriology.
- Baltimore: Williams Co, 1989. - Vol. 4. – P. 2451–2508.
16 Salha H.M., Al-Zahrani. Studies on the Antimicrobial Activity of Streptomyces // J. KAU Sci. – 2007. - Vol. 19. – P. 127-138.
17 Gupte M.D., Kulkarni P.R. A study of antifungal antibiotic production by Streptomyces chattanougensis MTCC 3423 using full factorial design // Lett. Appld. Microbiol. – 2002. – Vol. 35, № 1. – P. 22-26.
18 Vieira M.F., Sousa Q., Lopes C.E., Pereira N. J. Production of Actinomycin D by Streptomyces parvulus // Braz. Arch. Biol. Technol. - 2001. – Vol. 44, № 3. – P. 14-22.
19 ГОСТ 12038-84. Семена сельскохозяйственных культур. Методы определения всхожести. Изменение №2 к ГОСТ 12038-84 от 01.06.1995.
20 Урбах В.Ю. Статистический анализ в биологических и медицинских исследованиях. - М.: Медицина, 1975. - 295 с.
References
1 Joo GJ (2005) Production of an antifungal substance for biological control of Phytophthora capsici causing phytophthora blight in red-peppers by Streptomyces halstedii. Biotechnol Lett, 27:201–205.
2 Aryantha PG, Guest ID (2006) Mycoparasitic and antagonistic inhibition on Phytophtora cinnamomi Rands by microbial agents isolated from manure composts. Plant Pathology, 5:291-298.
3 Propagdee B, Kuekulvong C, Mongkolsuk S (2008) Antifungal potential of extracellular metabolites producer by Streptomyces hygroscopicus againts phytopathogenic fungi. Int J Biol Sci, 4:330-337.
4 Degtjareva EA, Vinogradova KA, Aleksandrova AV, Filonenko VA, Kozhevin PA (2009) Soil actinomycetes as potential fungicides. News of Moscow University, ser. soil science [Pochvennye aktinomicety kak potencial'nye biofungicidy. Vestnik Moskovskogo universiteta, ser. pochvovedenie] 2:22-26. (In Russian)
5 Snehal J, Shinde SK, Prashanthi PU, Krishnaraj J (2014) Identification and utilization of actinobacteria for biocontrol of rice sheath blight pathogen Rhizoctonia solani. Asian Journal of Bio Science, 9:227-233.
6 Bouras N, Meklat A, Toumatia O, Mokrane S, Holtz MD, Strelkov SE, Sabaou N (2013) Bioactive potential of a new strain of Streptomyces sp. PP14 isolated from Canadian soil. Afr J Microbiol Res, 7:3199-3208.
7 Shirokih IG e a (2013) The effects of the introduction of Streptomyces hygroscopicus A4 in phytosphere naked oats. Grain farm in Russia [Jeffekty introdukcii Streptomices hygroscopicus A4 v fitosferu golozernogo ovsa. Zernovoe hozjajstvo Rossii] 3:52-56. (In Russian)
8 Brown ME (1972) Plant growth substances produced by microorganisms of soil and rhizosphere. Journal of Applied Bacteriology, 35:443-451.
9 Martens DA, Frankenberger WT (1994) Assimilation of exogenous 2'-14C-indole acetic acid and 3'-14C-tryptophan exposed to the roots of three wheat varieties. Plant and Soil, 166:281-290.
10 Tokala RK, Strap JL, Jung CM, Crawford DL, Salove MH, Deobald LA, Bailey JF, Morra MJ (2002) Novel Plant-Microbe Rhizosphere Interaction Involving Streptomyces lydicus WYEC108 nd the Pea Plant (Pisum sativum). Applied and Environmental Microbiology, 68:2161-2171.
11 Waksman SA (1961) Classification, Identification and Description of Genera and Species. The Actinomycetes. Baltimore, Waverly Press.
12 Ilić S, Konstantinović S, Veljković B, Savić S, Gojgić-Cvijović G (2010) The impact of different carbon and nitrogen sources on antibiotic production by Streptomyces hygroscopicus CH-7. Current Research, Technology and Education Topics in Applied Microbiology and Microbial Biotechnology, 2:1337-1342.
13 Reddy NG, Ramakrishna D, Rajagopal S (2011) Optimization of culture conditions of Streptomyces rochei (MTCC 10109) for the production of antimicrobial metabolites. Egyptian Journal of Biology, 13:21-29.
14 Egorov NS (2004) Fundamentals of theory of antibiotics [Osnovy uchenija ob antibiotikah]. Moscow State University, Moscow, Russia (In Russian)
15 Locci R (1989) Streptomyces and Related Genera. Bergey’s Manual of Systematic Bacteriology. Baltimore: Williams Co, 4:2451–2508.
16 Salha HM, Al-Zahrani (2007) Studies on the Antimicrobial Activity of Streptomyces. J KAU Sci, 19:127-138.
17 Gupte MD, Kulkarni PR (2002) A study of antifungal antibiotic production by Streptomyces chattanougensis MTCC 3423 using full factorial design. Lett Appld Microbiol, 35:22-26.
18 Vieira MF, Sousa Q, Lopes CE, Pereira NJ (2001) Production of Actinomycin D by Streptomyces parvulus. Braz Arch Biol Technol, 44:14-22.
19 GOST 12038-84. Agricultural seeds. Methods for determining the germination. Change №2 to GOST 12038-84 from 01.06.1995 [GOST 12038-84. Semena sel'skohozjajstvennyh kul'tur. Metody opredelenija vshozhesti. Izmenenie #2 k GOST 12038-84 ot 01.06.1995] (In Russian)
20 Urbach VY (1975) Statistical analysis in biological and medical research [Statisticheskij analiz v biologicheskih i medicinskih issledovanijah]. Medicine, Moscow, Russia (In Russian)
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Trenozhnikova, L. P., Ultanbekova, G. D., Balgimbaeva, A. S., Galimbaeva, R. S., & Masirbaeva, A. (2016). Optimization of culture conditions for strains of the genus Streptomyces formulating biopreparation for plant cultivation. Experimental Biology, 67(2), 168–175. Retrieved from https://bb.kaznu.kz/index.php/biology/article/view/1192
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