Identification of oil-oxidizing microorganisms isolated from the water oil-bearing regions of the peninsula Buzachi
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Keywords:
autochthonous microflora, oil-oxidizing microorganisms, oil, oil products.Abstract
The objects of study were the cultures of bacteria and yeast isolated in the coastal zone of the Caspian Sea, adjacent to the oil fields on the peninsula Buzachi. 8 dominant cultures of microorganisms, demonstrating strong growth in medium containing crude oil, from local field, diesel and gasoline were isolated from water samples taken from peninsula Buzachi region of the Caspian Sea. Identification of these cultures according standard morphological, physiological and biochemical characteristics showed that they belong to the bacteria genera Pseudomonas, Bacillus, Rhodococcus, Ochrobactrum, Klebsiella, Lysinibacillus, Erwinia and the yeast genus Yarrowia.References
1 Гаджиев А.А., Шихшабеков М.М., Абдурахманов Г.М., Мунгиев А.А. Анализ экологического состояния Каспия и проблем воспроизводства рыб. - М.: Наука, 2003. - 424 с.
2 Куликова И.Ю. Микробиологическая оценка вод Северного Каспия в условиях освоения месторождений углеводородного сырья // Исследовано в России. – 2005. - №118. - С. 1190-1198.
3 Zhang D.C., Mörtelmaier C., Margesin R. Characterization of the bacterial archaeal diversity in hydrocarbon-contaminated soil // Sci Total Environ. - 2012. – № 1. – P. 184-196.
4 Margesin R., Schinner F. Biodegradation and bioremediation of hydrocarbons in extreme environments // Appl Microbiol Biotechnol. - 2001. - № 56(5-6). - P.650-663.
5 Nikolopoulou M., Pasadakis N., Norf H., Kalogerakis N. Enhanced ex situ bioremediation of crude oil contaminated beach sand by supplementation with nutrients and rhamnolipids // Mar Pollut Bull. – 2013. – № 77(1-2). - P. 37-44.
6 Antić M.P., Jovancićević B.S., Ilić M., Vrvić M.M., Schwarzbauer J. Petroleum pollutant degradation by surface water microorganisms // Environ Sci Pollut Res Int. – 2006. – № 13(5). – P. 320-327.
7 Das R., Kazy S.K. Microbial diversity, community composition and metabolic potential in hydrocarbon contaminated oily sludge: prospects for in situ bioremediation // Environ Sci Pollut Res Int. – 2014. - № 21(12). – Р. 7369-7389.
8 Нетрусов А.И., Егорова М.А., Захарчук Л.М., Колотилова Н.Н. и др. Практикум по микробиологии: учебное пособие для студентов высших учебных заведений. - М.: Академия, 2005. - 608 с.
9 Knieg N.R., Holf Y.G. Bergry’s Manual of Systematic Bacteriology. - London: Williams and Wilkins, 1984. - 154 р.
10 Wilson K. Preparation of genomic DNA from bacteria // Current Protocols in Molecular Biology. - 1987. - Р. 241-245.
11 Werle E., Schneider C., Renner M., Völker M., Fiehn W. Convenient single-step, one tube purification of PCR products for direct sequencing // Nucleic Acids Res. – 1994. – № 22. - P. 4354-4355.
12 Clayton R.A., Sutton G., Hinkle P.S., Bult Jr. C., Fields C. Intraspecific variation in small-subunit rRNA sequences in GenBank: why single sequences may not adequately represent prokaryotic taxa // International Journal of Systematic Bacteriology. – 1995. – № 45. – P. 595–599.
13 Pot B., Tsakalidou E. Taxonomy and Metabolism of Lactobacillus. Lactobacillus molecular biology from Genomics to Probiotics. - Norfolk UK: Caister Academik Press, 2009. - 206 p.
2 Куликова И.Ю. Микробиологическая оценка вод Северного Каспия в условиях освоения месторождений углеводородного сырья // Исследовано в России. – 2005. - №118. - С. 1190-1198.
3 Zhang D.C., Mörtelmaier C., Margesin R. Characterization of the bacterial archaeal diversity in hydrocarbon-contaminated soil // Sci Total Environ. - 2012. – № 1. – P. 184-196.
4 Margesin R., Schinner F. Biodegradation and bioremediation of hydrocarbons in extreme environments // Appl Microbiol Biotechnol. - 2001. - № 56(5-6). - P.650-663.
5 Nikolopoulou M., Pasadakis N., Norf H., Kalogerakis N. Enhanced ex situ bioremediation of crude oil contaminated beach sand by supplementation with nutrients and rhamnolipids // Mar Pollut Bull. – 2013. – № 77(1-2). - P. 37-44.
6 Antić M.P., Jovancićević B.S., Ilić M., Vrvić M.M., Schwarzbauer J. Petroleum pollutant degradation by surface water microorganisms // Environ Sci Pollut Res Int. – 2006. – № 13(5). – P. 320-327.
7 Das R., Kazy S.K. Microbial diversity, community composition and metabolic potential in hydrocarbon contaminated oily sludge: prospects for in situ bioremediation // Environ Sci Pollut Res Int. – 2014. - № 21(12). – Р. 7369-7389.
8 Нетрусов А.И., Егорова М.А., Захарчук Л.М., Колотилова Н.Н. и др. Практикум по микробиологии: учебное пособие для студентов высших учебных заведений. - М.: Академия, 2005. - 608 с.
9 Knieg N.R., Holf Y.G. Bergry’s Manual of Systematic Bacteriology. - London: Williams and Wilkins, 1984. - 154 р.
10 Wilson K. Preparation of genomic DNA from bacteria // Current Protocols in Molecular Biology. - 1987. - Р. 241-245.
11 Werle E., Schneider C., Renner M., Völker M., Fiehn W. Convenient single-step, one tube purification of PCR products for direct sequencing // Nucleic Acids Res. – 1994. – № 22. - P. 4354-4355.
12 Clayton R.A., Sutton G., Hinkle P.S., Bult Jr. C., Fields C. Intraspecific variation in small-subunit rRNA sequences in GenBank: why single sequences may not adequately represent prokaryotic taxa // International Journal of Systematic Bacteriology. – 1995. – № 45. – P. 595–599.
13 Pot B., Tsakalidou E. Taxonomy and Metabolism of Lactobacillus. Lactobacillus molecular biology from Genomics to Probiotics. - Norfolk UK: Caister Academik Press, 2009. - 206 p.
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Kalbayeva А. M., Tsurkan, Y. S., Karpenyuk, T. A., & Goncharova, A. V. (2016). Identification of oil-oxidizing microorganisms isolated from the water oil-bearing regions of the peninsula Buzachi. Experimental Biology, 65(3), 218–223. Retrieved from https://bb.kaznu.kz/index.php/biology/article/view/1120
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MICROBIOLOGY
