Detection of New Cytoplasmic RNA Corresponding to 5'-Terminal Fragment of 5.3 S RNA in Plant Cells
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Keywords:
SMALL CYTOPLASMIC RNAs, DESCRETE FRAGMENTATION, 18S rRNA, HEAT SHOCK.Abstract
It was revealed that in total RNA preparations isolated from wheat germ continuously detects small cytoplasmic RNA (scRNA) of about 55 nucleotides corresponding to the 5'-terminal fragment of 5.3S RNA, which in turn, is a fragment of 18S rRNA. This cytoplasmic RNA was detected in plant cells belonging to different taxa, as well as in rat liver cells, so that the investigated process of discrete 18S rRNA fragmentation should be sufficiently universal phenomenon. It was established that the content of investigated 18S rRNA fragment is much lower in young plant shoots rather than in dry and germinating seeds, and that its content increases if germinated seeds were exposed to heat shock (37° C). The process of discrete fragmentation of 18S rRNA probably could be the regulatory mechanism started in response to environmental stress.References
Список использованных источников
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(№ гос. Регистрации: 0113РК00354).
1. Baserga S.J. The Diverse World of Small Ribonucleoproteins // Cold Spring Harbor Lab. Press. - 2009. – P. 563. DOI: 10.1101/087969380.24.359.
2. Wilusz J.E., Freier S.M., Spector D.L. 3’ end processing of a long nuclear-retained non-coding RNA yields a tRNA-like cytoplasmic RNA // Cell. - 2008. - Vol. 135, No.5. – P. 919–932.
3. Zhanybekova S.Sh., Polimbetova N.S., Nakisbekov N.O., Iskakov, B.K. Detection in wheat germ ribosomes of a novel small RNA inducible by heat shock // Biochemistry (Moscow). – 1996. – Vol. 61, No. 5. – P. 621-627.
4. Johnston F.B., Stern H. Mass isolation of viable wheat embryo // Nature. -1957. - Vol. 179. - P. 160-161.
5. Martínez-Salas E., et al. Functional interactions in internal translation initiation directed by viral and cellular IRES elements // J.G. Virology. - 2001. - Vol. 82. - P. 973-84.
6. Manuell A.L., Yamaguchi K., Mayfield S.P. Composition and structure of the 80S ribosome from the green alga Chlamydomonas reinhardtii: 80S ribosomes are conserved in plants and animals // J. Mol. Biol. –2005. – Vol. 351. –P. 266-279.
(№ гос. Регистрации: 0113РК00354).
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Zhigailov, A. V., Kislitsin, V. Y., Polimbetova, N. S., & Iskakov, B. K. (2016). Detection of New Cytoplasmic RNA Corresponding to 5’-Terminal Fragment of 5.3 S RNA in Plant Cells. Experimental Biology, 64(2), 108–113. Retrieved from https://bb.kaznu.kz/index.php/biology/article/view/1070
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