Construction of recombinant Saccharomyces cerevisiae strains containing cellobiohydrolase gene of Lentinula edodes into the chromosomal HO locus.

Authors

  • S. M. Taipakova НИИ проблем биологии и биотехнологии, КазНУ им. аль-Фараби, г. Алматы
  • I. T. Smekenov НИИ проблем биологии и биотехнологии, КазНУ им. аль-Фараби, г. Алматы
  • A. K. Bissenbaev НИИ проблем биологии и биотехнологии, КазНУ им. аль-Фараби, г. Алматы

Keywords:

cellobiohydrolase, Lentinula edodes, Saccharomyces cerevisiae, genome integration, α-factor,

Abstract

Using genetic engineering techniques the pHO-GAPDH-α-cel7A-myc-6His-KanMX4-HO integrated construction, including cellobiohydrolase gene cel7A of the Lentinula edodes with the signal sequence of yeast α-factor, Mycepitope and 6xHis tail have been designed. The new stable yeast strain efficiently expressing recombinant cellobiohydrolase CEL7A of the fungus Lentinula edodes have been obtained. Chromosomal integration of the cel7A gene at the HO locus was confirmed by PCR verification. Keywords:

Issue

Vol. 60 No. 2 (2014): KazNU Bulletin. Biology series

Section

BIOTECHNOLOGY

How to Cite

Construction of recombinant Saccharomyces cerevisiae strains containing cellobiohydrolase gene of Lentinula edodes into the chromosomal HO locus. (2015). Experimental Biology, 60(2), 352-355. https://bb.kaznu.kz/index.php/biology/article/view/971

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