Construction of recombinant Saccharomyces cerevisiae strains containing endo-1,4-β-glucanase gene of Aspergillus niger into the chromosomal HO locus

Abstract

The vector carrying a selective marker gene resistant to geneticin (G418), a constitutive promoter and terminator of yeast gene glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was created for integration of genetic material into the chromosomal HO locus. Through the use of a genomic integration vector, new yeast strains with efficient expression of endo-1,4-â-glucanase of the fungus Aspergillus niger were obtained. Approaches used in this study has been shown to be perspective in the development of industrial strains for the fermentation of cellulose-containing materials.