СВЯЗЫВАНИЕ МЕЖГЕННЫХ microRNA ЧЕЛОВЕКА С САЙТАМИ mRNA ГЕНОВ, УЧАСТВУЮЩИХ В РАЗВИТИИ РАКА ТОЛСТОЙ КИШКИ

Abstract

Binding sites of 784 intergenic miRNAs with mRNAs of 54 genes involving in colorectal cancer development were established. Significant difference of miRNA binding sites between 5’UTR, CDS and 3'UTR of mRNA was revealed. Average density of sites for 5'UTR of 40 genes is 33,1 s/l and it is 6,5, 4,7 and 3,8 times more than in 3'UTR, CDS and whole mRNA respectively. Many of intergenic miRNA bind to several mRNA in some sites. For 40 target genes of ig-miRNAs length ratio was, on the average, 4,4%, 59,9% and 29,4% for 5'UTR, CDS and 3'UTR respectively, the proportion of sites interacting with ig-miRNA was 22,4%, 49,2% and 28,4% for with 5'UTR, CDS and 3'UTR respectively. It follows from our research results that miRNAs might regulate gene expression by interaction to 3'UTR and affect on 5'UTR and CDS too.