Запасные белки зерновки как маркеры в селекции первого казахстанского сорта риса с окрашенным перикарпом
Ключевые слова:
рис с окрашенным перикарпом, гибриды, запасные белки.Аннотация
ӨББИ коллекциясының 11 сорттарына, 9 коллекциялық үлгілеріне және 12 гибридтеріне белоктық паспортизация жүргізілді. Қор белоктарының электрофорезі өзгертілген Laemmli әдісі бойынша жүргізілді. Күріш оризениндерін бөліп алу және фракциялау жағдайлары оңтайландырылды. Зерттеу жұмыстарының нәтижесінде қызыл дәнді Ко 296 R үлгісінде басқа қызыл дәнді формаларда айқын байқалмаған молекулалық массасы шамамен 55 kDа болатын қарқынды белок спектрі анықталды. Перикарпы боялған Қара күріш генотипінің қор белоктары спектрінде молекулалық салмағы 55 және 60 kDа тең белоктық компоненттер айқындалды, бұл белоктық компоненттер генотиптің басқа формалардан ерекшелігін көрсетеді. ӨББИ селекциясындағы F1 гибридтерінің «шынайылығы» ата-аналық формалардың компоненттерінің бар болуымен дәлелденді. Проведена белковая паспортизация 11 сортов, 9 коллекционных образцов и 12 гибридов риса из коллекции ИББР. Электрофорез запасных белков проводили модифицированным методом Laemmli. Подобраны оптимальные условия выделения и фракционирования оризенинов риса. В результате проведенных работ у краснозерного образца Ко 296 R выявлены интенсивные белковые спектры с молекулярной массой около 55 kDа, тогда как у других краснозерных форм данный компонент слабо выражено. В спектре запасных белков семян у генотипа Черный рис (Китай) с антоциановой окраской наблюдается проявление интенсивных белковых полос с молекулярной массой 55 и 60 kDа, что подтверждает сортспецифичность данного генотипа. Подтверждена «истинность» гибридов F1 поколений из 12 комбинаций селекции ИББР наличием компонентов обеих родительских форм. Seed storage protein identification of 11 rice varieties, 9 collection samples and 12 hybrids from the IPBB collection were conducted. Electrophoresis of storage proteins was carried out by method of Laemmli. Optimum conditions were selected for isolation and fractionation of rice glutelin. As a result of this research in red grain sample Ko 296 R revealed intense protein spectra with molecular weight of about 55 kDa, whereas the other red grain forms of this component weakly expressed. In the spectrum of seed storage proteins in genotype Black rice (China) with anthocyanin coloration observed manifestation of intense protein bands with molecular weights of 55 and 60 kDa, which confirms specificity of this genotype.The "verity" of F1 hybrids of 12 combinations from the IPBB breeding was confirmed by the presence of components of both parental forms.Библиографические ссылки
Список использованных источников:
1 Furukawa T., Maekawa M., Oki T., Suda I., Iida S., Shimada H., Takamure I., Kadowaki K. The Rc and Rd genes are involved in proanthocyanidin synthesis in rice pericarp // Plant. -2006. - J 49:91-102.
2 Yawadio R., Tanimori S. and Morita N. Identification of phenolic compounds isolated from pigmented rice’s and their aldose reductase inhibitory activities // Food Chemistry. - 2007. - 101 (4): 1616-1625.
3 Iqbal S., Bhanger M.I., Anwar F. Antioxidant properties and components of some commercially available varieties of rice bran in Pakistan // Food Chemistry. - 2005. - 93 (2): 265-272.
4 Zhang M.W., Guo B.J., Zhang R.F., Chi J.W., Wei Z.C., Xu Z.H., Zhang Y., Tang X.J. Separation, purification and identification of antioxidant compositions in black rice // Agricultural Science in China. - 2006. - 5 (6): 431-440.
5 Hu C., Zawistowski J., Ling W., Kitts, D.D. Black Rice (Oryza sativa L. indica) Pigmented Fraction Suppresses both Reactive Oxygen Species and Nitric Oxide in Chemical and Biological Model Systems // Journal of Agriculture and Food Chemistry. - 2003. - (51): 5271-5277.
6 Bechtel D.B., Juliano B.O. Formation of protein bodies in the starchy endosperm of rice (Oryza sativa L.): A re-investigation // Ann. Bot. 1980.- 45. – Р.503-509.
7 Krishnan H.B., Franceschi V.R., Okita T.W. Immunochemical studies on the role of the golgi complex in protein-body formation in rice seeds // Planta - 1986. - 169. - Р. 471-480.
8 Yamagata H., Tanaka K. The site of synthesis and accumulation of rice storage proteins // Plant Cell Physiol. - 1986. - 27. - Р. 135-145.
9 Tanaka K., Sugimoto T., Ogawa, M., Kasai Z. Isolation and characterization of two types of protein bodies in rice endosperm // Agric. Biol. Chem. - 1980 - 44. - Р.1633-1639.
10 Cagampang G.B. Studies on the extraction and composition of rice proteins // Cereal Chem. - 1966. - V. 43. - N. 2. - P.145-155.
11 Kawakatsu T., Yamamoto M.P., Hirose S., Yano M., Takaiwa F. Characterization of a new rice glutelin gene GluD-1 expressed in the starchy endosperm // J. Exp. Bot.- 2008. - 59. - Р. 4233-4245.
12 Yoon, U.H.; Lee, J.H.; Hahn, J.H.; Kim, Y.K.; Lee, G.S.; Ji, H.S.; Kim, C.K.; Mun, J.H.; Kim, Y.M.; Kim, T.H. Structural and expression analysis of prolamin genes in Oryza sativa L. // Plant Biotechnol. Rep. - 2012. - 6. - Р. 251-262.
13 Muench D.G., Ogawa M., Okita T.W. The prolamins of rice. In Seed Proteins, 2nd ed.; Springer Netherlands: Dordrecht, The Netherlands, - 1999. - Р. 93-108.
14 Ogawa M., Kumamaru T., Satoh H., Iwata N., Omura T., Kasai Z., Tanaka K. Purification of protein body-I of rice seed and its polypeptide composition // Plant Cell Physiol. - 1987. - 28. - Р.1517-1527.
15 Li X., Okita T.W. Accumulation of prolaminesand glutelins during rice seed development: A quantitative-evaluation // Plant Cell Physiol. - 1993. - 34. - Р. 385-390.
16 Shorrosh B.S., Wen L., Zen K.C., Huang J.K., Pan J.S., Hermodson M.A., Tanaka K., Muthukrishnan S., Reeck G.R. A novel cereal storage protein: Molecular genetics of the 19 kDa globulin of rice // Plant Mol. Biol. - 1992. - 18. - Р. 151-154.
17 Krishnan H.B., White J.A., Pueppke S.G. Characterization and localization of rice (Oryza sativa L.) seed globulins // Plant Sci. - 1992. - 81. - Р.1-11.
18 Булатова К.М. Изучение компонентного состава глютенина пшеницы // Вестник с.-х. науки Казахстана. - 1985. - №4. - С.37-39.
19 Lu Z.M., Wang H, Shen Y.J. Polymorphism of endosperm storage protein and application in hybrid rice (Oryza sativa L.) // J. Nanjing Agric.Univ., - 2001. - V. 24, - №2.- P.6-11.
20 Sarker R., Bose S. Electrophoresis characterization of rice varieties using single seed (salt soluble) proteins // Theor. Appl. Genet. - 1984. - V.68. - P.415-419.
21 Prathepha P., Daipolmak V., Samappito S., Baimai V. An assessment of alkali degradation, Waxy protein and their relation to amylase content in Thai rise cultivars // Science Asia.- 2005.-V.31. - P.69-75.
22 Hong D.L., Satob H., Kumammaru T., Qu L.Q., Sadar U.S. Protein markers of BT type hybrid and its three lines in japonica rice (Oryza sativa L.) // Chinese J. Rice Sci. - 2001. - V.16. - 3.-P.165-168.
23 Конарев В.Г. Идентификация сортов и регистрация генофонда культурных растений по белкам семян. - СПб., 2000. - 186 с.
References
1 Furukawa T., Maekawa M., Oki T., Suda I., Iida S., Shimada H., Takamure I., Kadowaki K. The Rc and Rd genes are involved
in proanthocyanidin synthesis in rice pericarp // Plant. -2006. – J 49:91-102.
2 Yawadio R., Tanimori S. and Morita N. Identification of phenolic compounds isolated from pigmented rice’s and their aldose
reductase inhibitory activities // Food Chemistry. – 2007. – 101 (4): 1616-1625.
3 Iqbal S., Bhanger M.I., Anwar F. Antioxidant properties and components of some commercially available varieties of rice
bran in Pakistan // Food Chemistry. – 2005. – 93 (2): 265-272.
4 Zhang M.W., Guo B.J., Zhang R.F., Chi J.W., Wei Z.C., Xu Z.H., Zhang Y., Tang X.J. Separation, purification and identification
of antioxidant compositions in black rice // Agricultural Science in China. – 2006. – 5 (6): 431-440.
5 Hu C., Zawistowski J., Ling W., Kitts, D.D. Black Rice (Oryza sativa L. indica) Pigmented Fraction Suppresses both Reactive
Oxygen Species and Nitric Oxide in Chemical and Biological Model Systems // Journal of Agriculture and Food Chemistry.
– 2003. – (51): 5271-5277.
6 Bechtel D.B., Juliano B.O. Formation of protein bodies in the starchy endosperm of rice (Oryza sativa L.): A re-investigation
// Ann. Bot. 1980.- 45. – Р.503-509.
7 Krishnan H.B., Franceschi V.R., Okita T.W. Immunochemical studies on the role of the golgi complex in protein-body formation in rice seeds // Planta – 1986. – 169. – Р. 471-480.
8 Yamagata H., Tanaka K. The site of synthesis and accumulation of rice storage proteins // Plant Cell Physiol. – 1986. – 27. – Р.
135-145.
9 Tanaka K., Sugimoto T., Ogawa, M., Kasai Z. Isolation and characterization of two types of protein bodies in rice endosperm
// Agric. Biol. Chem. – 1980 – 44. – Р.1633-1639.
10 Cagampang G.B. Studies on the extraction and composition of rice proteins // Cereal Chem. – 1966. – V. 43. – N. 2. – P.145-
155.
11 Kawakatsu T., Yamamoto M.P., Hirose S., Yano M., Takaiwa F. Characterization of a new rice glutelin gene GluD-1 expressed
in the starchy endosperm // J. Exp. Bot.- 2008. – 59. – Р. 4233-4245.
12 Yoon, U.H.; Lee, J.H.; Hahn, J.H.; Kim, Y.K.; Lee, G.S.; Ji, H.S.; Kim, C.K.; Mun, J.H.; Kim, Y.M.; Kim, T.H. Structural and
expression analysis of prolamin genes in Oryza sativa L. // Plant Biotechnol. Rep. – 2012. – 6. – Р. 251-262.
13 Muench D.G., Ogawa M., Okita T.W. The prolamins of rice. In Seed Proteins, 2nd ed.; Springer Netherlands: Dordrecht, The
Netherlands, – 1999. – Р. 93-108.
14 Ogawa M., Kumamaru T., Satoh H., Iwata N., Omura T., Kasai Z., Tanaka K. Purification of protein body-I of rice seed and
its polypeptide composition // Plant Cell Physiol. – 1987. – 28. – Р.1517-1527.
15 Li X., Okita T.W. Accumulation of prolaminesand glutelins during rice seed development: A quantitative-evaluation // Plant
Cell Physiol. – 1993. – 34. – Р. 385-390.
16 Shorrosh B.S., Wen L., Zen K.C., Huang J.K., Pan J.S., Hermodson M.A., Tanaka K., Muthukrishnan S., Reeck G.R. A novel cereal storage protein: Molecular genetics of the 19 kDa globulin of rice // Plant Mol. Biol. – 1992. – 18. – Р. 151-154.
17 Krishnan H.B., White J.A., Pueppke S.G. Characterization and localization of rice (Oryza sativa L.) seed globulins // Plant
Sci. – 1992. – 81. – Р.1-11.
18 Bulatov K.M. The study of the component composition of wheat glutenin // Bulletin of Agricultural Science of Kazakhstan.
– 1985. – №4. – Р.37-39.
19 Lu Z.M., Wang H, Shen Y.J. Polymorphism of endosperm storage protein and application in hybrid rice (Oryza sativa L.) // J.
Nanjing Agric.Univ., – 2001. – V. 24, – №2.- P.6-11.
20 Sarker R., Bose S. Electrophoresis characterization of rice varieties using single seed (salt soluble) proteins // Theor. Appl.
Genet. – 1984. – V.68. – P.415-419.
21 Prathepha P., Daipolmak V., Samappito S., Baimai V. An assessment of alkali degradation, Waxy protein and their relation to amylase content in Thai rise cultivars // Science Asia.- 2005.-V.31. – P.69-75.
22 Hong D.L., Satob H., Kumammaru T., Qu L.Q., Sadar U.S. Protein markers of BT type hybrid and its three lines in japonica
rice (Oryza sativa L.) // Chinese J. Rice Sci. – 2001. – V.16. – 3.-P.165-168.
23 Konarev V.G. Identification and registration of varieties of the gene pool of cultivated plants by seed protein. – SPb., 2000. – 186 p.
1 Furukawa T., Maekawa M., Oki T., Suda I., Iida S., Shimada H., Takamure I., Kadowaki K. The Rc and Rd genes are involved in proanthocyanidin synthesis in rice pericarp // Plant. -2006. - J 49:91-102.
2 Yawadio R., Tanimori S. and Morita N. Identification of phenolic compounds isolated from pigmented rice’s and their aldose reductase inhibitory activities // Food Chemistry. - 2007. - 101 (4): 1616-1625.
3 Iqbal S., Bhanger M.I., Anwar F. Antioxidant properties and components of some commercially available varieties of rice bran in Pakistan // Food Chemistry. - 2005. - 93 (2): 265-272.
4 Zhang M.W., Guo B.J., Zhang R.F., Chi J.W., Wei Z.C., Xu Z.H., Zhang Y., Tang X.J. Separation, purification and identification of antioxidant compositions in black rice // Agricultural Science in China. - 2006. - 5 (6): 431-440.
5 Hu C., Zawistowski J., Ling W., Kitts, D.D. Black Rice (Oryza sativa L. indica) Pigmented Fraction Suppresses both Reactive Oxygen Species and Nitric Oxide in Chemical and Biological Model Systems // Journal of Agriculture and Food Chemistry. - 2003. - (51): 5271-5277.
6 Bechtel D.B., Juliano B.O. Formation of protein bodies in the starchy endosperm of rice (Oryza sativa L.): A re-investigation // Ann. Bot. 1980.- 45. – Р.503-509.
7 Krishnan H.B., Franceschi V.R., Okita T.W. Immunochemical studies on the role of the golgi complex in protein-body formation in rice seeds // Planta - 1986. - 169. - Р. 471-480.
8 Yamagata H., Tanaka K. The site of synthesis and accumulation of rice storage proteins // Plant Cell Physiol. - 1986. - 27. - Р. 135-145.
9 Tanaka K., Sugimoto T., Ogawa, M., Kasai Z. Isolation and characterization of two types of protein bodies in rice endosperm // Agric. Biol. Chem. - 1980 - 44. - Р.1633-1639.
10 Cagampang G.B. Studies on the extraction and composition of rice proteins // Cereal Chem. - 1966. - V. 43. - N. 2. - P.145-155.
11 Kawakatsu T., Yamamoto M.P., Hirose S., Yano M., Takaiwa F. Characterization of a new rice glutelin gene GluD-1 expressed in the starchy endosperm // J. Exp. Bot.- 2008. - 59. - Р. 4233-4245.
12 Yoon, U.H.; Lee, J.H.; Hahn, J.H.; Kim, Y.K.; Lee, G.S.; Ji, H.S.; Kim, C.K.; Mun, J.H.; Kim, Y.M.; Kim, T.H. Structural and expression analysis of prolamin genes in Oryza sativa L. // Plant Biotechnol. Rep. - 2012. - 6. - Р. 251-262.
13 Muench D.G., Ogawa M., Okita T.W. The prolamins of rice. In Seed Proteins, 2nd ed.; Springer Netherlands: Dordrecht, The Netherlands, - 1999. - Р. 93-108.
14 Ogawa M., Kumamaru T., Satoh H., Iwata N., Omura T., Kasai Z., Tanaka K. Purification of protein body-I of rice seed and its polypeptide composition // Plant Cell Physiol. - 1987. - 28. - Р.1517-1527.
15 Li X., Okita T.W. Accumulation of prolaminesand glutelins during rice seed development: A quantitative-evaluation // Plant Cell Physiol. - 1993. - 34. - Р. 385-390.
16 Shorrosh B.S., Wen L., Zen K.C., Huang J.K., Pan J.S., Hermodson M.A., Tanaka K., Muthukrishnan S., Reeck G.R. A novel cereal storage protein: Molecular genetics of the 19 kDa globulin of rice // Plant Mol. Biol. - 1992. - 18. - Р. 151-154.
17 Krishnan H.B., White J.A., Pueppke S.G. Characterization and localization of rice (Oryza sativa L.) seed globulins // Plant Sci. - 1992. - 81. - Р.1-11.
18 Булатова К.М. Изучение компонентного состава глютенина пшеницы // Вестник с.-х. науки Казахстана. - 1985. - №4. - С.37-39.
19 Lu Z.M., Wang H, Shen Y.J. Polymorphism of endosperm storage protein and application in hybrid rice (Oryza sativa L.) // J. Nanjing Agric.Univ., - 2001. - V. 24, - №2.- P.6-11.
20 Sarker R., Bose S. Electrophoresis characterization of rice varieties using single seed (salt soluble) proteins // Theor. Appl. Genet. - 1984. - V.68. - P.415-419.
21 Prathepha P., Daipolmak V., Samappito S., Baimai V. An assessment of alkali degradation, Waxy protein and their relation to amylase content in Thai rise cultivars // Science Asia.- 2005.-V.31. - P.69-75.
22 Hong D.L., Satob H., Kumammaru T., Qu L.Q., Sadar U.S. Protein markers of BT type hybrid and its three lines in japonica rice (Oryza sativa L.) // Chinese J. Rice Sci. - 2001. - V.16. - 3.-P.165-168.
23 Конарев В.Г. Идентификация сортов и регистрация генофонда культурных растений по белкам семян. - СПб., 2000. - 186 с.
References
1 Furukawa T., Maekawa M., Oki T., Suda I., Iida S., Shimada H., Takamure I., Kadowaki K. The Rc and Rd genes are involved
in proanthocyanidin synthesis in rice pericarp // Plant. -2006. – J 49:91-102.
2 Yawadio R., Tanimori S. and Morita N. Identification of phenolic compounds isolated from pigmented rice’s and their aldose
reductase inhibitory activities // Food Chemistry. – 2007. – 101 (4): 1616-1625.
3 Iqbal S., Bhanger M.I., Anwar F. Antioxidant properties and components of some commercially available varieties of rice
bran in Pakistan // Food Chemistry. – 2005. – 93 (2): 265-272.
4 Zhang M.W., Guo B.J., Zhang R.F., Chi J.W., Wei Z.C., Xu Z.H., Zhang Y., Tang X.J. Separation, purification and identification
of antioxidant compositions in black rice // Agricultural Science in China. – 2006. – 5 (6): 431-440.
5 Hu C., Zawistowski J., Ling W., Kitts, D.D. Black Rice (Oryza sativa L. indica) Pigmented Fraction Suppresses both Reactive
Oxygen Species and Nitric Oxide in Chemical and Biological Model Systems // Journal of Agriculture and Food Chemistry.
– 2003. – (51): 5271-5277.
6 Bechtel D.B., Juliano B.O. Formation of protein bodies in the starchy endosperm of rice (Oryza sativa L.): A re-investigation
// Ann. Bot. 1980.- 45. – Р.503-509.
7 Krishnan H.B., Franceschi V.R., Okita T.W. Immunochemical studies on the role of the golgi complex in protein-body formation in rice seeds // Planta – 1986. – 169. – Р. 471-480.
8 Yamagata H., Tanaka K. The site of synthesis and accumulation of rice storage proteins // Plant Cell Physiol. – 1986. – 27. – Р.
135-145.
9 Tanaka K., Sugimoto T., Ogawa, M., Kasai Z. Isolation and characterization of two types of protein bodies in rice endosperm
// Agric. Biol. Chem. – 1980 – 44. – Р.1633-1639.
10 Cagampang G.B. Studies on the extraction and composition of rice proteins // Cereal Chem. – 1966. – V. 43. – N. 2. – P.145-
155.
11 Kawakatsu T., Yamamoto M.P., Hirose S., Yano M., Takaiwa F. Characterization of a new rice glutelin gene GluD-1 expressed
in the starchy endosperm // J. Exp. Bot.- 2008. – 59. – Р. 4233-4245.
12 Yoon, U.H.; Lee, J.H.; Hahn, J.H.; Kim, Y.K.; Lee, G.S.; Ji, H.S.; Kim, C.K.; Mun, J.H.; Kim, Y.M.; Kim, T.H. Structural and
expression analysis of prolamin genes in Oryza sativa L. // Plant Biotechnol. Rep. – 2012. – 6. – Р. 251-262.
13 Muench D.G., Ogawa M., Okita T.W. The prolamins of rice. In Seed Proteins, 2nd ed.; Springer Netherlands: Dordrecht, The
Netherlands, – 1999. – Р. 93-108.
14 Ogawa M., Kumamaru T., Satoh H., Iwata N., Omura T., Kasai Z., Tanaka K. Purification of protein body-I of rice seed and
its polypeptide composition // Plant Cell Physiol. – 1987. – 28. – Р.1517-1527.
15 Li X., Okita T.W. Accumulation of prolaminesand glutelins during rice seed development: A quantitative-evaluation // Plant
Cell Physiol. – 1993. – 34. – Р. 385-390.
16 Shorrosh B.S., Wen L., Zen K.C., Huang J.K., Pan J.S., Hermodson M.A., Tanaka K., Muthukrishnan S., Reeck G.R. A novel cereal storage protein: Molecular genetics of the 19 kDa globulin of rice // Plant Mol. Biol. – 1992. – 18. – Р. 151-154.
17 Krishnan H.B., White J.A., Pueppke S.G. Characterization and localization of rice (Oryza sativa L.) seed globulins // Plant
Sci. – 1992. – 81. – Р.1-11.
18 Bulatov K.M. The study of the component composition of wheat glutenin // Bulletin of Agricultural Science of Kazakhstan.
– 1985. – №4. – Р.37-39.
19 Lu Z.M., Wang H, Shen Y.J. Polymorphism of endosperm storage protein and application in hybrid rice (Oryza sativa L.) // J.
Nanjing Agric.Univ., – 2001. – V. 24, – №2.- P.6-11.
20 Sarker R., Bose S. Electrophoresis characterization of rice varieties using single seed (salt soluble) proteins // Theor. Appl.
Genet. – 1984. – V.68. – P.415-419.
21 Prathepha P., Daipolmak V., Samappito S., Baimai V. An assessment of alkali degradation, Waxy protein and their relation to amylase content in Thai rise cultivars // Science Asia.- 2005.-V.31. – P.69-75.
22 Hong D.L., Satob H., Kumammaru T., Qu L.Q., Sadar U.S. Protein markers of BT type hybrid and its three lines in japonica
rice (Oryza sativa L.) // Chinese J. Rice Sci. – 2001. – V.16. – 3.-P.165-168.
23 Konarev V.G. Identification and registration of varieties of the gene pool of cultivated plants by seed protein. – SPb., 2000. – 186 p.
Выпуск
Раздел
Экспериментальная биология. Биолог-кие осн. генофонда и развитие науч. исслед. в обл. селек-генетич. исслед. с/х культур
